Journal Biomed最新文献_第5页

Experimental Evaluation of the Pharmacological Safety of a New Propagandic Acid Derivative with a Cardiotropic Action 对具有强心作用的新型丙酸衍生物药理安全性的实验评估

Journal Biomed Pub Date : 2023-11-20 DOI: 10.33647/2713-0428-19-3e-95-98

D. Ivkin, A. A. Karpov, V. V. Kovanskov, I. A. Titovich

引用次数: 0

Genetic Screening of a New Transgenic Mouse Line Humanized for HLA-A*02:01:01:01 and hβ2m 人源化 HLA-A*02:01:01:01 和 hβ2m 新转基因小鼠品系的遗传筛选

Journal Biomed Pub Date : 2023-11-19 DOI: 10.33647/2713-0428-19-3e-10-24

N. Karkischenko, E. S. Glotova, N. V. Petrova, V. Slobodenyuk, N. A. Laryushina, D. V. Petrov, I. A. Vasil’eva, K. E. Deryabin

引用次数: 0

A Vitamin B9-based Dietary Supplement Normalizes the Expression of Lipid Metabolism Regulatory Genes in a Mouse Model of a High-cholesterol Diet 维生素 B9 膳食补充剂可使高胆固醇饮食小鼠模型中的脂质代谢调节基因表达正常化

Journal Biomed Pub Date : 2023-11-19 DOI: 10.33647/2713-0428-19-3e-25-30

A. E. Burova, A. P. Reykh, A. V. Gorlova, E. P. Svirin, K. N. Zabegalov, K. D. Chaprov, A. E. Umrukhin, T. V. Strekalova

{"title":"A Vitamin B9-based Dietary Supplement Normalizes the Expression of Lipid Metabolism Regulatory Genes in a Mouse Model of a High-cholesterol Diet","authors":"A. E. Burova, A. P. Reykh, A. V. Gorlova, E. P. Svirin, K. N. Zabegalov, K. D. Chaprov, A. E. Umrukhin, T. V. Strekalova","doi":"10.33647/2713-0428-19-3e-25-30","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-25-30","url":null,"abstract":"The Western Diet (WD) is a nutritional style characterized by excessive intake of cholesterol, saturated fatty acids and sugars; this nutritional pattern can cause type 2 diabetes, metabolic syndrome and other disorders, particularly during ageing. The search for effective approaches to managing the metabolic syndrome caused by WD seems to be a relevant research task. Unfortunately, this issue has attracted insufficient attention in the current literature. In this work, we use a mouse model of WD to study potential effects of a food supplement based on dicholine succinate (DS) and folic acid (vitamin B9), which are activators of mitochondrial functions. We study glucose tolerance, parameters of hippocampus-dependent learning and relative gene expression in RT-PCR of molecular markers of negative WD effects in the brain and liver of aging mice housed on WD. Mice C57BL/6 were 12 months old and housed on WD for 3 weeks; some groups received В9 (5 mg/kg/day) or DS (150 mg/kg/day), or their combination, via water. We carried out food displacement and fear conditioning learning tests followed by RT-PCR of several genes in the liver and brain. We found a decreased glucose tolerance, an elevated speed of pellet displacement and a reduction of freezing time in the fear conditioning test. This may suggest cognitive deficits and impulsivity of mice housed on WD. The administration of DS and B diminished most of these changes. In addition, the increased expression of FASN in the liver points to new mechanisms of negative WD effects during aging. The food supplement based on B9 and DS normalizes FASN expression and behavior, as well as glucose tolerance in WD-housed mice. Our results open new perspectives for further studies of therapeutic and preventive effects of food supplements on the regulation of metabolic parameters during ageing.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"8 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139260654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Issues of the Use and Efficacy of Direct Oral Anticoagulants According their Pharmacogenetic Features 直接口服抗凝血剂的药物遗传学特点及其使用和疗效现状

Journal Biomed Pub Date : 2023-06-10 DOI: 10.33647/2074-5982-19-2-37-44

E. Gantsgorn, A. Safronenko, E. V. Gubin, V. Vlasenko, E. V. Rashkova, I. M. Malleev, A. V. Ivanov, S. Gerasyuta, G. Bulguryan, YaO Osipenko, O. V. Denisenko, D. A. Saakyan, M. H. Ivanova

引用次数: 0

Results of a Phase I Open-Label Clinical Trial of Direkord in Healthy Volunteers 在健康志愿者中进行的一项I期开放标签临床试验结果

Journal Biomed Pub Date : 2023-06-10 DOI: 10.33647/2074-5982-19-2-69-77

I. Pomytkin, V. Pisarev, M. E. Merkulov, S. M. Noskov, N. Karkischenko

{"title":"Results of a Phase I Open-Label Clinical Trial of Direkord in Healthy Volunteers","authors":"I. Pomytkin, V. Pisarev, M. E. Merkulov, S. M. Noskov, N. Karkischenko","doi":"10.33647/2074-5982-19-2-69-77","DOIUrl":"https://doi.org/10.33647/2074-5982-19-2-69-77","url":null,"abstract":"Direkord is an original drug containing the active substance of dicholine succinate, which enhances neuronal insulin sensitivity. In this work, we study the tolerability, safety, and pharmacokinetic parameters of dicholine succinate when administered intramuscularly in a phase I clinical trial in healthy volunteers. In total, 18 healthy volunteers –11 men and 7 women – with a mean age of 30.4±7.8 years, were recruited into a randomized study. At stage I, 6 volunteers (group 1) received dicholine succinate intramuscularly every other day with a dose escalation from 0.16 mg/kg/day to 600 mg/day. At stage II, 12 volunteers (group 2) received dicholine succinate intramuscularly at a single dose of 200 mg, and then, at stage III, the same 12 volunteers received dicholinesuccinate at a dose of 600 mg/day (3 x 200 mg at an interval of 8 hours) for seven days. The safety population in this study included all randomized volunteers. Data from 12 volunteers (group 2) were included in the calculation of the pharmacokinetic parameters. All volunteers completed all procedures of the three research stages in accordance with the protocol. According to clinical and laboratory monitoring data, no adverse events were registered during the study. The drug was well tolerated, with no signs of hyperemia, edema, and bruising being observed at the injection site. The volunteers did not complain of pain, itching, and burning. After a single injection of dicholine succinate, the concentration of choline in the bloodstream reached its maximum value after an average of 0.375±0.365 hours with the half-life of 1.271±1.071 hours. After repeated administration at a dose of 600 mg per day, no cumulation of the active substance was observed. The data obtained have confirmed a good safety profile of Direkord; therefore, the drug can be recommended for further investigation in a study involving patients.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84560399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein, Lipid and Carbohydrate-Energy Metabolism Indices in Rats under Acute and Chronic Hypokinetic Stress and their Combinations 急性和慢性低运动应激大鼠蛋白质、脂肪和碳水化合物能量代谢指标及其组合

Journal Biomed Pub Date : 2023-06-10 DOI: 10.33647/2074-5982-19-2-16-26

M. Ravaeva, I. Cheretaev, E. Chuyan, P. A. Galenko-Yaróshevskii

引用次数: 0

Cardiorespiratory Endurance is Associated with Metabolic Syndrome Severity in Men 男性心肺耐力与代谢综合征严重程度相关

Journal Biomed Pub Date : 2023-06-10 DOI: 10.33647/2074-5982-19-2-61-68

V. Sverchkov, E. Bykov

{"title":"Cardiorespiratory Endurance is Associated with Metabolic Syndrome Severity in Men","authors":"V. Sverchkov, E. Bykov","doi":"10.33647/2074-5982-19-2-61-68","DOIUrl":"https://doi.org/10.33647/2074-5982-19-2-61-68","url":null,"abstract":"Maintaining a good level of fitness through regular exercise is essential for the treatment and prevention of metabolic syndrome (MS). However, the question of which components of physical fitness have the greatest impact remains controversial. We studied the relationship between cardiorespiratory endurance and MS severity z-score in men. The study involved 44 untrained men (38.7±5.6 years). Anthropometric parameters (height, weight, BMI), glucose level, triglyceride level, high-density lipoprotein level, blood pressure, and MS severity z-score were assessed. We also assessed the level of cardiorespiratory endurance in a 12-minute Cooper test. The results of our study showed an inverse relationship between the z-score of MS severity and cardiorespiratory endurance in men, which was (r=–0.84; p˂0.05). People in the highest quartile of cardiorespiratory endurance (quartile 1) had a significantly lower MS severity z-score compared to people in the lowest quartile of cardiorespiratory endurance (quartile 4) (p˂0.01). In addition, people who demonstrated the highest cardiorespiratory endurance (quartile 1) had statistically significantly lower fasting plasma glucose levels and abdominal girth (p˂0.05), as well as statistically significantly lower levels of triglycerides, systolic blood pressure and higher levels of high-density lipoprotein (p˂0.01) compared to people in the lowest quartile of cardiorespiratory endurance (quartile 4). The obtained data confirm the protective role of cardiorespiratory endurance against MS in men.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73868962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmaco-EEG Analysis of Regulatory Neuropeptides with Nootropic Properties in Cats 猫促智性调节性神经肽的药物-脑电图分析

Journal Biomed Pub Date : 2023-06-10 DOI: 10.33647/2074-5982-19-2-8-15

Y. Fokin, S. Kharitonov, N. Karkischenko

引用次数: 0

Sex Differences in Addictive Behavior of Adult Rats: Effects of Prenatal Alcohol Exposure 成年大鼠成瘾行为的性别差异:产前酒精暴露的影响

Journal Biomed Pub Date : 2023-06-10 DOI: 10.33647/2074-5982-19-2-27-36

P. Anokhin, T. Proskuryakova, V. Shokhonova, V. Kokhan, I. Tarabarko, I. Shamakina

{"title":"Sex Differences in Addictive Behavior of Adult Rats: Effects of Prenatal Alcohol Exposure","authors":"P. Anokhin, T. Proskuryakova, V. Shokhonova, V. Kokhan, I. Tarabarko, I. Shamakina","doi":"10.33647/2074-5982-19-2-27-36","DOIUrl":"https://doi.org/10.33647/2074-5982-19-2-27-36","url":null,"abstract":"Alcohol experienced during gestation is associated with the development of neurodevelopmental and neuropsychiatric dysfunctions, as well as addictive behavior in the offspring. However, the biological basis of these effects remains poorly understood. Taking into account that the extrahypothalamic corticotropin-releasing factor (CRF) system plays an important role in regulation of the negative emotional state produced by alcohol abuse and withdrawal, the present study was aimed at investigating: 1) the effect of prenatal alcohol exposure (PA) on voluntary alcohol drinking (free choice 24 hours/day) or intermittent (“drinking in the dark”) regimen in adult Wistar rats; 2) differences in the basal gene expression levels of CRF and CRF-R1 in amygdala of adult PA and control rats; and 3) the effect of voluntary alcohol drinking on the above mRNA levels. PA males displayed a significantly greater voluntary alcohol intake than control males as observed by both drinking paradigms. 24 hours after the first withdrawal episode, PA males demonstrated a higher level of anxiety in the light-dark box test. No differences were found between PA and control females. Basal amygdalar CRF and CRFR1 mRNA levels did not differ between PA and control rats of both sexes. No difference was observed in the amygdalar CRF and CRFR1 mRNA levels after alcohol drinking in PA and control males. Conversely, the CRF mRNA levels in amygdala of PA female rats decreased under the action of alcohol consumption, compared to control female rats. The results show that the PA effect on future alcohol-related behavior is sex-specific, but do not support the hypothesis that changes in CRF and CRFR1 mRNA levels in amygdala may be responsible for high alcohol intake in males.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77134457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study into the Efficacy of the Epophen Substance in Normobaric Hypoxia Epophen物质在常压缺氧中的作用研究

Journal Biomed Pub Date : 2023-06-10 DOI: 10.33647/2074-5982-19-2-45-53

K. Ostrenko, A. Ovcharova, O. P. Yegorova

引用次数: 0