Japanese journal of bacteriology最新文献

{"title":"Recent Aspects of the Studies of Anaerobes","authors":"Shoichiro Suzuki","doi":"10.3412/JSB.23.1","DOIUrl":"https://doi.org/10.3412/JSB.23.1","url":null,"abstract":"","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"312 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"1968-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74559959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on the antitumor activity of polysaccharide produced by a strain of Crepidotus sp.","authors":"H. Nakayoshi","doi":"10.3412/JSB.22.641","DOIUrl":"https://doi.org/10.3412/JSB.22.641","url":null,"abstract":"An extracellular polysaccharide isolated from the supernatant of broth culture of a Crepidotus B-145 was soluble in water, giving a highly viscous solution. Estimation of molecular weight with light scattering gave the value of 1.52×166, while the calculation from determination of reducing endogroup gave the value of 1.6-3.6×104, suggesting the formation of molecular aggregates in solution.Analyses of its component sugar with thin layer chromatography and gas-liquid chromatograhy revealed that the polysaccharide was composed largely from glucose. By gas chromatographic analysis of its permethylation-methanolysis products, it was found that the polysaccharide has 1-3 glucosidic linkage in its structure. Infra red spectroscopy indicated that the linkage is of β-configuration.The presence of β 1-3 linkage was further confirmed by the cleavage with β 1-3 glucanase.Interestingly, the treatment with the polysaccharide was found to suppress the growth of Sarcoma 37 in mice as expected from the antitumor activity of yeast glucan, the active structure of which was said to be β 1-3 linkage.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"13 1","pages":"115-119"},"PeriodicalIF":0.0,"publicationDate":"1968-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84548366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthomonas tmnslucens f. sp. hordei Hagborgによるオーチャードグラスの黄枯細菌病","authors":"時任 富永","doi":"10.3412/JSB.22.628","DOIUrl":"https://doi.org/10.3412/JSB.22.628","url":null,"abstract":"In June of 1966, a new bacterial disease was found causing brownish streaks on the foliage of orchardgrass (Dactylis glomerutaL.) in Japan. The causal organism was identified as Xanthomonas translucens f. sp. hordei Hagborg, considering its bacteriological characters, symptom on the leaf-blade showing the characteristic translucency and pathogenic specialization. It is the first time that X. translucens has been found to attack orchardgrass naturally and some of wheatgrasses (Agropyronspp.) artificially.The bacteriological characters of the pathogen are as follows: Cylindrical rods rounded at ends, Solitary or in pairs; 0.6×1.2-2.4μ in size, motile with a single flagellum; capsulate, Gram negative, no spores, aerobic. Superficial colonies, yellow, round, convex, smooth, shining, opaque, with entire margin. Liquefies gelatin slowly; digests milk slowly without coagulation; nitrates not reduced; hydrogen sulphide and ammonia produced, but not indol or acetoin; starch hydrolysed variable. Acid but no gass produced from arabinose, xylose, glucose, fructose, galactose, mannose, lactose, sucrose and glycerol: neither acid nor gas from rhamnose, maltose, raffinose, starch, inulin, dextrin, mannitol, sorbitol or salicin. Aesculin hydrolysed; oxidase-positive; lipolytic. According to Hugh-Leifson's method, acid produced aerobically from glucose. Optimum temperature 30°C.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"78 1","pages":"628-633"},"PeriodicalIF":0.0,"publicationDate":"1967-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90219191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"易溶性感染防御抗原(SPA)の免疫学的研究 (1)","authors":"田中 哲之助, 嗣郎 小林, 久義 杉原, 利部 光四郎","doi":"10.3412/JSB.22.583","DOIUrl":"https://doi.org/10.3412/JSB.22.583","url":null,"abstract":"","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"25 1","pages":"583-589"},"PeriodicalIF":0.0,"publicationDate":"1967-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73983221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treponema pallidum Reiter株の抗原構造分析と梅毒血清反応における意義","authors":"Yorihiro Saijo","doi":"10.3412/JSB.22.510","DOIUrl":"https://doi.org/10.3412/JSB.22.510","url":null,"abstract":"","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"24 1","pages":"510-518"},"PeriodicalIF":0.0,"publicationDate":"1967-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83286109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"大腸菌O-111, B4による食中毒について","authors":"鈴木 ミツヱ, 賢治 原田, 達夫 松山","doi":"10.3412/jsb.22.326","DOIUrl":"https://doi.org/10.3412/jsb.22.326","url":null,"abstract":"The authors performen the bacteriological and epidemiological observations on the food poisoning incidence in October, 1966, which was caused by a strain of E. coli.Epidemiological observations revealed that the number of patients, with principal symptoms of stomachache, nausea, vomitting, diarrhea and fever, 15 with 18.5% of morbidity. the patients were all adult and the mean time of incubation period was 10.5h.E. coli strains were isolated almost in pure culture on the media used diagnostically from 5 patients examined. These cultures originated as single lactose-fermenting colonies on the media, which were there-after subcultured and identified by fermentative and biochemical reactions as Escherichia coli. These cultures were also identified by biological and serological reactions as E. coil O-111, B4 and agreed with the biological properties of type 1 described by F. Kauffmann. Widal reaction did not show any rise in O- antibody against the strain E. coil O-111, B4 which was isolated from the patients.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"26 1","pages":"326-328"},"PeriodicalIF":0.0,"publicationDate":"1967-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87334953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ジヒドロチオクト酸によるマムシ(Agkistorodon halys)毒のトキソイド化に関する基礎的研究","authors":"敏夫 清水","doi":"10.3412/JSB.22.312","DOIUrl":"https://doi.org/10.3412/JSB.22.312","url":null,"abstract":"Sawai et al. (1966) previously reported inactivation of venom of the habu (Trimeresurus flavoviridis) by dihydrothioctic acid (DHTA). In this study, effectiveness of DHTA in inactivating mamushi venom was investigated.The results showed that the hemorrhagic and necrotic action of mamushi venom were also prevented by DHTA which was added to the venom in equal or half amount and incubated at 37 degree C for one hour, and injected intramuscularly into the legs of white mice, subcutaneously into the plantar side of legs of mice or intramuscularly into rabbits.Mice and rabbits were then, immunized with mamushi venom treated with equal amount of DHTA. After four weeks, 3 boosters of the same amounts of toxoided venom were given at the interval of one week. And the neutralizing effects of the sera of immunized animals were tested. They were also challenged intramuscularly into the legs with the venoms. The results indicated that 0.1ml of sera of immunized animals neutralized from four to sixteen mhds (minimum hemorrhagic dose) by the method of intramuscular or intracutaneous injection.After the direct challenge of venom on immunized animals, it was also indicated that the local lesion of treated animals were decreased in considerable degree compared with control animals. Serum treatment of envenomated rabbits which were previously given toxoided venom were more successful than the animals without toxoid treatment.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"48 1","pages":"312-320"},"PeriodicalIF":0.0,"publicationDate":"1967-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83457342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"抗日本脳炎ウイルス血清の2-Mercaptoethanol感受性に関する研究 (II)","authors":"Satoru Otsuka, K. Manako, Ryoichi Mori, Hidefumi Kunihiro, Ichiro Motomura","doi":"10.3412/JSB.22.250","DOIUrl":"https://doi.org/10.3412/JSB.22.250","url":null,"abstract":"Further studies were carried out to investigate the 2-mercaptoethanol (2ME)-sensitivity of JE virus antibody of pigs. The results obtained are as follows:1. In the natural infection cases, 2ME-sensitive antibodies were detected during the first 2 to 4 weeks of antibody response; thereafter the antibodies were found to be resistant to 2ME treatment.2. Five pigs have been subjected to the observation of 2ME-sensitivity of the sera for 2 or 3 consecutive years. Sera obtained from one pig at the third oversummering were found to be sensitive to 2ME treatment, i.e., the hemagglutination inhibition (HI) titers of the sera were reduced to a quarters of the original titers by 2ME treatment. However, the 2ME-sensitivity of antibodies of other cases which experienced the second or third oversummering, were found to be 2ME-resistant as before.3. In the experimental vaccination cases, the first antibodies produced after vaccination were found to be 2ME-sensitive and thereafter they became 2ME-resistant.4. Three pigs possessing 2ME-sensitive HI antibodies were detected in 173 cases possessing antibodies during the interepidemic period. Those were found in March, April and October in 1965, respectively.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"70 1","pages":"250-255"},"PeriodicalIF":0.0,"publicationDate":"1967-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77285730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on the Surface Structure of Fungi","authors":"I. Tani, M. Kuriyama, T. Otsuka","doi":"10.3412/JSB.22.634","DOIUrl":"https://doi.org/10.3412/JSB.22.634","url":null,"abstract":"The cell wall of many fungi contains chitin a polymer of N-acetylglucosamine. During the course of a study concerned with the surface structure of fungi, a number of fungal whole cells and their purified cell walls were analysed to measure their chitin contents. This report presents the results of quantitative analyses of chitin in several fungi and their susceptibility to phenol in relation to the changes of chitin content followed by the length of incubation (4-20 days).The results are as follows.1) Both the glucosamine hydrochloride obtained by hydrolysis of the chitin and standard D-glucosamine hydrochloride revealed identical absorption curves showing a distinct maximum value at 530mμ. The results are shown in Fig. 2.2) For this experiment, 4 fungal strains (Asperillus niger IAM 2020, Geotrichum candidum IFO 6454, Penicillium chrysogenum IFO 4626, Trichophyton rubrum IFO 5467) were used. Chitin contents of dry whole cell and purified cell wall were estimated quantitatively on each of 4, 8, 12, 16 and 20 daycultures which were grown in either synthetic or Sabouraud's medium by shaking culture. It was found that the chitin content increased gradually with the day of incubation. In addition to this, the chitin contents of 8 and 16 days old culture of all fungal strains were determined in purified preparations of cell walls which were obtained by means of sonic treatment. The purity of the cell wall was examined with several microscopic methods, such as fluorescence microscopy, phase-contrast microscopy and electron microscopy. The results are shown in Figs. 3 and 4, Photos 1 and 2 and Table 1.3) During 4-20 days incubation time of fungi, the changes of pH in the medium and the dry weight of ethanol precipitate of culture filtrate were estimated. The results are shown in Fig. 5 and Table 2.4) Without regard to the age of cultures or their chitin content, the susceptibility to phenol of two fungi (i.e. A. niger IAM 2020, G. candidum IFO 6454) was not changed. The results are shown in Table 3.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"24 1","pages":"191-199"},"PeriodicalIF":0.0,"publicationDate":"1967-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90863492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on Micrococcus Fasteus, a New Micrococcal Species, Isolated from Human Skin Lesions","authors":"M. Soeda, M. Otomo, M. Ome","doi":"10.3412/JSB.22.305","DOIUrl":"https://doi.org/10.3412/JSB.22.305","url":null,"abstract":"Many cases of staphylococcal infection resistant to antibiotic therapy have been successfully treated by the use of toxoid prepared from resistant Staphylococcus aureus strains in our laboratory. In recent one year, we have encountered occasional cases with skin lesions which do not respond to such toxoid therapy in spite of the fact that morphologically staphylococci-like microorganisms can be isolated from the corresponding lesions almost in pure state.We have collected 9 strains from patients with chronic, skin lesions which did not respond to both antibiotics and staphylococcal toxoid, and examined their detailed biological and serological properties in order to know the reason why they being resistant to such therapies.These cocci are spherical cells similar to staphylococci, but they are usually arranged in dipro or tetra forms and never be grouped in irregular clusters as often seen in Staphylococcus aureus or epidermidis. In addition, they are not uniform in size and tend to be somewhat larger than staphylococci. For these reasons they are not so difficult to identify from staphylococci by simple microscopic examination of directstained smears from the lesions or their cultures.Their biological properties are quite similar to those of staphylococci except for being incapable of producing nitrite from nitrate. They are variable in the coagulase test and almost all strains are resistant to the antibiotics, particularly to penicillin. Their colonies on agar media are also similar in appearance to those of staphylococci and cannot be identified by simple inspection.A soluble, thermolabile exotoxin is produced by them which causes tissue necrosis or death in experimental animals and heamolyzes rabbit erythrocytes. This exotoxin is antigenic like staphylococcal exotoxin and gives rise to a specific antitoxin which neutralyzes it, and can be altered to toxoid form by treatment with formalin according to the method used for preparation of staphylococcal toxoid.We have prepared M. fasteus toxoid adopting the representative strains and applied it to clinical cases for therapeutic purpose of chronic skin lesions uncontrollable by antibiotics or staphylococcal toxoid which proved capable of inducing complete disappearance of M. fasteus in a short period, which was followed by the rapid healing of the skin lesions.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"16 1","pages":"305-311"},"PeriodicalIF":0.0,"publicationDate":"1967-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77737299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}