ジヒドロチオクト酸によるマムシ(Agkistorodon halys)毒のトキソイド化に関する基礎的研究

Sawai et al. (1966) previously reported inactivation of venom of the habu (Trimeresurus flavoviridis) by dihydrothioctic acid (DHTA). In this study, effectiveness of DHTA in inactivating mamushi venom was investigated.The results showed that the hemorrhagic and necrotic action of mamushi venom were also prevented by DHTA which was added to the venom in equal or half amount and incubated at 37 degree C for one hour, and injected intramuscularly into the legs of white mice, subcutaneously into the plantar side of legs of mice or intramuscularly into rabbits.Mice and rabbits were then, immunized with mamushi venom treated with equal amount of DHTA. After four weeks, 3 boosters of the same amounts of toxoided venom were given at the interval of one week. And the neutralizing effects of the sera of immunized animals were tested. They were also challenged intramuscularly into the legs with the venoms. The results indicated that 0.1ml of sera of immunized animals neutralized from four to sixteen mhds (minimum hemorrhagic dose) by the method of intramuscular or intracutaneous injection.After the direct challenge of venom on immunized animals, it was also indicated that the local lesion of treated animals were decreased in considerable degree compared with control animals. Serum treatment of envenomated rabbits which were previously given toxoided venom were more successful than the animals without toxoid treatment.