Journal - Association of Official Analytical Chemists最新文献_第4页

Analysis of organochlorine pesticide residues using simultaneous injection of two capillary columns with electron capture and electrolytic conductivity detectors. 电子捕获电导检测器双毛细管柱同时进样分析有机氯农药残留。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01

M L Hopper

引用次数: 0

Assay of oxolinic acid residues in salmon muscle by liquid chromatography with fluorescence detection: interlaboratory study. 荧光液相色谱法测定鲑鱼肌肉中氧喹啉酸残留量:实验室间研究。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01

G Carignan, L Larocque, S Sved

{"title":"Assay of oxolinic acid residues in salmon muscle by liquid chromatography with fluorescence detection: interlaboratory study.","authors":"G Carignan, L Larocque, S Sved","doi":"","DOIUrl":"","url":null,"abstract":"A previously developed method that uses a simplified sample preparation and fluorometric detection of liquid chromatographic eluates for the determination of oxolinic acid in salmon muscle has been collaboratively studied. Five laboratories participated in the study to analyze, in quintuplicate, blank salmon muscle fortified at 10, 20, 50, and 100 micrograms/kg (ppb), and 2 incurred samples from salmon given feed with medicated oxolinic acid. The tissue, 2 g mixed with 2 g Na2SO4, is extracted with ethyl acetate and centrifuged, and the solvent is evaporated. The residue is partitioned in a mixture of hexane and 0.01 M oxalic acid, and the aqueous phase is chromatographed using fluorescence detection at 327 nm excitation and 369 nm emission. Mean recoveries ranged from 77.2 to 84.5% in spiked samples with reproducibility relative standard deviation (RSDR) ranging from 11.5 to 18.3%. Treated salmon were found to contain 8.71 and 53.8 micrograms/kg with RSDR of 18.6 and 16.7%, respectively. The corresponding repeatability relative standard deviations (RSDR) were 5.8-12.2%, and 7.7 and 6.2%. The method is recommended for regulatory purposes in Canada.","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 6","pages":"906-9"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12920992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic determination of free glutamic acid in dried soups and in minced sausages: NMKL collaborative study. 酶法测定干汤和碎香肠中游离谷氨酸:NMKL合作研究。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01 DOI: 10.1093/JAOAC/74.6.921

M. Hattula, H. Wallin

引用次数: 3

Liquid chromatographic determination of paralytic shellfish poisons in shellfish after prechromatographic oxidation. 色谱前氧化贝类中麻痹性贝类毒素的液相色谱测定。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01 DOI: 10.1093/JAOAC/74.6.1006

J. F. Lawrence, C. Ménard

{"title":"Liquid chromatographic determination of paralytic shellfish poisons in shellfish after prechromatographic oxidation.","authors":"J. F. Lawrence, C. Ménard","doi":"10.1093/JAOAC/74.6.1006","DOIUrl":"https://doi.org/10.1093/JAOAC/74.6.1006","url":null,"abstract":"A liquid chromatographic method for quantitating paralytic shellfish poison toxins in shellfish has been developed in which the toxins are converted to fluorescent purines by prechromatographic oxidation under mildly basic conditions with hydrogen peroxide or periodate. The addition of ammonium formate to the periodate oxidation reaction greatly improved the yield of fluorescent derivatives for neosaxitoxin, gonyautoxin-1, B-2, and C-3 compared to the same reaction without ammonium formate. As little as 3-6 ng of each of the nonhydroxylated toxins and 7-12 ng of the hydroxylated compounds per gram of shellfish could be detected. Reversed-phase chromatography using ammonium formate in the mobile phase improved the chromatography of neosaxitoxin and B-2 compared to results obtained earlier. Because the oxidation products of neosaxitoxin and B-2 could not be separated, parent compounds were separated before oxidation by using an SPE-COOH ion exchange cartridge. The repeatability coefficient of variation for the oxidation reactions ranged from 3 to 8% for the peroxide reaction, and from 4 to 11% for the periodate reaction, depending upon the individual toxin determined and its concentration in the extract (0.04-0.55 micrograms/g). The method was compared to the mouse bioassay and the postcolumn oxidation method. In most cases, results were comparable.","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"19 1","pages":"1006-12"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76941892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 72

Use of the Mycosep multifunctional cleanup column for liquid chromatographic determination of aflatoxins in agricultural products. 应用Mycosep多功能净化柱液相色谱法测定农产品中黄曲霉毒素。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01

T J Wilson, T R Romer

{"title":"Use of the Mycosep multifunctional cleanup column for liquid chromatographic determination of aflatoxins in agricultural products.","authors":"T J Wilson, T R Romer","doi":"","DOIUrl":"","url":null,"abstract":"A liquid chromatographic (LC) technique has been developed that uses the Mycosep multifunctional cleanup (MFC) column. MFC columns provide a rapid 1-step extract purification. They are designed to retain particular groups of compounds that may create interferences in analytical methods. At the same time, MFC columns allow compounds of interest to pass through. In the method presented, test samples are extracted in a blender with acetonitrile-water (9 + 1). A portion of the extract is forced through an MFC column designed especially for analysis of numerous mycotoxins. Analytical interferences are retained, while aflatoxins pass through the column. Aflatoxins B1 and G1 are converted to their hemiacetals by heating a mixture of purified extract and water-trifluoroacetic acid-acetic acid (7 + 2 + 1) at 65 degrees C for 8.5 min. An aliquot of this mixture is analyzed by isocratic LC with acetonitrile-water mobile phase and fluorescence detection. A detection limit of less than 0.5 ng/g for aflatoxin B1 was obtained. Average recoveries greater than 95% total aflatoxins (B1, B2, G1, and G2) and coefficients of variation of less than 3% were obtained. The method was successfully applied to the following commodities: corn, almonds, pista-chios, walnuts, peanuts, Brazil nuts, milo, rice, cottonseed, corn meal, corn gluten meal, fig paste, and mixed feeds.","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 6","pages":"951-6"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12920236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supercritical fluid extraction/enzyme assay: a novel technique to screen for pesticide residues in meat products. 超临界流体萃取/酶分析:一种筛选肉制品中农药残留的新技术。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01

J E France, J W King

引用次数: 0

Identification of whale species by thin-layer isoelectric focusing of sarcoplasmic proteins. 用肌浆蛋白薄层等电聚焦法鉴定鲸鱼种类。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01 DOI: 10.1093/JAOAC/74.6.943

Y. Ukishima, M. Kino, H. Kubota, S. Wada, S. Okada

{"title":"Identification of whale species by thin-layer isoelectric focusing of sarcoplasmic proteins.","authors":"Y. Ukishima, M. Kino, H. Kubota, S. Wada, S. Okada","doi":"10.1093/JAOAC/74.6.943","DOIUrl":"https://doi.org/10.1093/JAOAC/74.6.943","url":null,"abstract":"Thin-layer isoelectric focusing was applied to the identification of whale (Cetacea) species by using water-soluble sarcoplasmic proteins of skeletal muscles. Twenty-eight samples consisting of 4 species (10 samples) of baleen whales (Mysticeti) and 8 species (18 samples) of toothed whales (Odontoceti) were analyzed. Each sample (approximately 1 g) was electrophoresed with Ampholine PAGplate, pH 3.5-9.5. The electrophoretic profiles were species-specific on the 4 toothed whale species that did not have a marked intra-species difference, and all 4 baleen whale species. However, the profiles were not specific on the 4 other dolphin species, even though they were discriminable from the other 4 toothed whale species. Numerical values of pIs and relative peak heights were obtained by densitometric analysis of the isoelectro-focused protein bands. The bands were also species-specific for the 8 toothed whale species mentioned. The values may be applicable to species identification without the need for a standard sample, which may not be readily obtainable. Experiments on test samples of minke and sel whales showed that bloodletting with ice water made the densities of isoelectro-focused bands thinner, although species identification was still possible by using the inside part of muscles. Heat treatment at below 60 degrees C for 10 min caused little denaturation; at higher temperatures the protein bands were diminished in a temperature-dependent fashion. Therefore, the present isoelectric focusing analysis should be applicable to small samples of whale meat, excluding several species of dolphins.","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"48 1","pages":"943-50"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89975916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Analysis of trypsin inhibitors and lectins in white kidney beans (Phaseolus vulgaris, var. Processor) in a combined method. 联合方法分析白芸豆(Phaseolus vulgaris, var. Processor)中胰蛋白酶抑制剂和凝集素。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01

J P Roozen, J de Groot

引用次数: 0

Polyvinyl chloride matrix membrane electrodes for manual and flow injection analysis of chloroquine in pharmaceutical preparations. 用于药物制剂中氯喹手工和流动注射分析的聚氯乙烯基质膜电极。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01

S S Hassan, M A Ahmed

引用次数: 0

Identification of whale species by thin-layer isoelectric focusing of sarcoplasmic proteins. 用肌浆蛋白薄层等电聚焦法鉴定鲸鱼种类。

Journal - Association of Official Analytical Chemists Pub Date : 1991-11-01

Y Ukishima, M Kino, H Kubota, S Wada, S Okada

{"title":"Identification of whale species by thin-layer isoelectric focusing of sarcoplasmic proteins.","authors":"Y Ukishima, M Kino, H Kubota, S Wada, S Okada","doi":"","DOIUrl":"","url":null,"abstract":"Thin-layer isoelectric focusing was applied to the identification of whale (Cetacea) species by using water-soluble sarcoplasmic proteins of skeletal muscles. Twenty-eight samples consisting of 4 species (10 samples) of baleen whales (Mysticeti) and 8 species (18 samples) of toothed whales (Odontoceti) were analyzed. Each sample (approximately 1 g) was electrophoresed with Ampholine PAGplate, pH 3.5-9.5. The electrophoretic profiles were species-specific on the 4 toothed whale species that did not have a marked intra-species difference, and all 4 baleen whale species. However, the profiles were not specific on the 4 other dolphin species, even though they were discriminable from the other 4 toothed whale species. Numerical values of pIs and relative peak heights were obtained by densitometric analysis of the isoelectro-focused protein bands. The bands were also species-specific for the 8 toothed whale species mentioned. The values may be applicable to species identification without the need for a standard sample, which may not be readily obtainable. Experiments on test samples of minke and sel whales showed that bloodletting with ice water made the densities of isoelectro-focused bands thinner, although species identification was still possible by using the inside part of muscles. Heat treatment at below 60 degrees C for 10 min caused little denaturation; at higher temperatures the protein bands were diminished in a temperature-dependent fashion. Therefore, the present isoelectric focusing analysis should be applicable to small samples of whale meat, excluding several species of dolphins.","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 6","pages":"943-50"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12920995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0