Iranian journal of allergy, asthma, and immunology最新文献_第7页

Correlation of Expression of MMP-2, ACE2, and TMPRSS2 Genes with Lymphopenia for Mild and Severity of COVID-19. MMP-2、ACE2和TMPRSS2基因表达与COVID-19轻、重度淋巴细胞减少的相关性

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-02-20 DOI: 10.18502/ijaai.v22i1.12011

Behrooz Ghezelbash, Mehdi Rostami, Mohammad Heidarvand, Alireza Mafi, Hamid Chegni, Nahid Eskandari

{"title":"Correlation of Expression of MMP-2, ACE2, and TMPRSS2 Genes with Lymphopenia for Mild and Severity of COVID-19.","authors":"Behrooz Ghezelbash, Mehdi Rostami, Mohammad Heidarvand, Alireza Mafi, Hamid Chegni, Nahid Eskandari","doi":"10.18502/ijaai.v22i1.12011","DOIUrl":"https://doi.org/10.18502/ijaai.v22i1.12011","url":null,"abstract":"Some risk causes may be associated with the severity of COVID-19. The central host-pathogen factors might affect infection are human receptor angiotensin-converting enzyme 2 (ACE2), trans-membrane protease serine 2 (TMPRSS2), and SARS-CoV-2 surface spike (S)-protein. The main purpose of this study was to determine the differences in the expression the metalloproteinases-2 (MMP-2), MMP-9, ACE2, and TMPRSS2 genes and their correlation with lymphopenia in the mild and severe types of the COVID-19 patients. Eighty-eight patients, aged 36 to 60 years old with the mild (n=44) and severe (n=44) types of COVID-19 were enrolled. Total RNA was isolated from the peripheral blood mononuclear cells (PBMCs). The changes of MMP-2, MMP-9, ACE2 and TMPRSS2 gene expression in PBMCs from mild and severe COVID-19 patients were examined by the real time-quantitative polymerase chain reaction (RT-qPCR) assay and, compared between the groups. Data were collected from May 2021 to March 2022. The mean age of the patients in both groups was 48 (interquartile range, 36-60), and there were no appreciable differences in age or gender distribution between the two groups. The present study showed that a significant increase in the expression of ACE2, TMPRSS2, MMP-2, and MMP-9 genes in the severe type of the COVID-19 patients compared, to the mild type of the COVID-19 patients. Overall, it suggests the expression levels of these genes on the PBMC surface in the immune system are susceptible to infection by SARS-COV-2 and therefore could potentially predict the patients' outcome.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9239396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Risk of the Next Child Getting Affected by Chronic Granulomatous Disease in Families with at Least One Autosomal Recessive CGD Child. 至少有一个常染色体隐性CGD儿童的家庭下一个孩子患慢性肉芽肿病的风险

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-02-20 DOI: 10.18502/ijaai.v22i1.12014

Seyedeh Zalfa Modarresi, Shagayegh Tajik, Mohsen Badalzadeh, Mohammad Reza Fazlollahi, Massoud Houshmand, Marzieh Maddah, Zahra Alizadeh, Mohammad Nabavi, Nasrin Bazargan, Masoud Movahedi, Zahra Pourpak

{"title":"The Risk of the Next Child Getting Affected by Chronic Granulomatous Disease in Families with at Least One Autosomal Recessive CGD Child.","authors":"Seyedeh Zalfa Modarresi, Shagayegh Tajik, Mohsen Badalzadeh, Mohammad Reza Fazlollahi, Massoud Houshmand, Marzieh Maddah, Zahra Alizadeh, Mohammad Nabavi, Nasrin Bazargan, Masoud Movahedi, Zahra Pourpak","doi":"10.18502/ijaai.v22i1.12014","DOIUrl":"https://doi.org/10.18502/ijaai.v22i1.12014","url":null,"abstract":"Chronic granulomatous disease (CGD) is a rare primary immunodeficiency disorder more common in autosomal recessive (AR) than X-linked in Iran. This study aimed to assess whether having a child with AR-CGD would increase the likelihood of the next child being affected by CGD. Ninety-one families with at least one child affected by AR-CGD entered this study. Out of the 270 children, 128 were affected by AR-CGD. We used a cross tab for the odds ratio (OR) calculation, in which exposure to a previously affected child and the next child's status were evaluated. This study illustrated that the chances of having another child afflicted with AR-CGD are significantly increased if the previous child had AR-CGD (OR=2.77, 95% CI=1.35-5.69).Althoug h AR disorders affect 25% of each pregnancy, we showed that the chance that the next child would be affected by CGD, given that the previous child was affected, is 2.77 times greater than in families with a normal child. It is recommended to warn families with one or more affected children to evaluate the risk of CGD in their subsequent pregnancies with prenatal diagnosis.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9239907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Killer Cell Immunoglobulin-like Receptor (KIR) Genes and their HLA Ligands with Susceptibility to Takayasu Arteritis in the Iranian Population. 伊朗人群中杀伤细胞免疫球蛋白样受体(KIR)基因及其HLA配体与高松动脉炎易感性的关系

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-02-20 DOI: 10.18502/ijaai.v22i1.12003

Fereshteh Beigmohammadi, Saeed Aslani, Hoda Kavosi, Ali Javinani, Shayan Mostafaei, Mehran Pournazari, Baharak Tasorian, Elham Farhadi, Asghar Hajiabbasi, Habib Zayeni, Alireza Khabbazi, Ahmadreza Jamshidi, Irandokht Shenavar Masooleh, Zahra Tamartash, Mahdi Vojdanian, Mahdi Mahmoudi

{"title":"Association of Killer Cell Immunoglobulin-like Receptor (KIR) Genes and their HLA Ligands with Susceptibility to Takayasu Arteritis in the Iranian Population.","authors":"Fereshteh Beigmohammadi, Saeed Aslani, Hoda Kavosi, Ali Javinani, Shayan Mostafaei, Mehran Pournazari, Baharak Tasorian, Elham Farhadi, Asghar Hajiabbasi, Habib Zayeni, Alireza Khabbazi, Ahmadreza Jamshidi, Irandokht Shenavar Masooleh, Zahra Tamartash, Mahdi Vojdanian, Mahdi Mahmoudi","doi":"10.18502/ijaai.v22i1.12003","DOIUrl":"https://doi.org/10.18502/ijaai.v22i1.12003","url":null,"abstract":"Takayasu arteritis (TA) is a chronic inflammatory disorder characterized by vascular damage and fibrosis in the intima that commonly occurs in the aorta. In many damaged sites in TA patients, natural killer (NK) cells have been shown to be hyperactivated and produce inflammatory cytokines and toxic components. Killer cell immunoglobulin-like receptors (KIRs) are found on NK cells and interact with human leukocyte antigen (HLA) class I ligands to activate or suppress NK cells. The present study assessed the possible role of KIR and their HLA ligand genes in susceptibility to TA in Iranian patients. This case-control study included 50 TA patients and 50 healthy subjects. DNA was extracted from whole peripheral blood samples, and polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed to recognize the presence or absence of polymorphism in 17 KIR genes and 5 HLA class I ligands in each participant. Among the KIR and HLA genes, a significant decrease was detected in the frequency of 2DS4 (full allele) in TA patients (38%) compared with healthy controls (82%) (OR=0.13, 95% CI=0.05-0.34). However, none of the KIR and HLA genotypes or the interactions between these genes were associated with susceptibility to TA. The KIR2DS4 gene might be involved in the regulation of activation as well as the production of cytotoxic mediators of NK cells in patients with TA.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9582760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific Clinical and Immunological Changes Following Mesenchymal Stem Cell Transplantation in COVID-19-induced Acute Respiratory Distress Syndrome Patients: A Phase-I Clinical Trial. 新冠肺炎急性呼吸窘迫综合征患者间充质干细胞移植后特异性临床和免疫学变化:一项i期临床试验

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2022-12-24 DOI: 10.18502/ijaai.v21i6.11530

Najmeh Kaffash Farkhad, Alireza Sedaghat, Hamidreza Reihani, Amir Adhami Moghadam, Ahmad Bagheri Moghadam, Nayereh Khadem Ghaebi, Mohammad Ali Khodadoust, Amin Reza Nikpoor, Jalil Tavakol Afshari

{"title":"Specific Clinical and Immunological Changes Following Mesenchymal Stem Cell Transplantation in COVID-19-induced Acute Respiratory Distress Syndrome Patients: A Phase-I Clinical Trial.","authors":"Najmeh Kaffash Farkhad, Alireza Sedaghat, Hamidreza Reihani, Amir Adhami Moghadam, Ahmad Bagheri Moghadam, Nayereh Khadem Ghaebi, Mohammad Ali Khodadoust, Amin Reza Nikpoor, Jalil Tavakol Afshari","doi":"10.18502/ijaai.v21i6.11530","DOIUrl":"https://doi.org/10.18502/ijaai.v21i6.11530","url":null,"abstract":"Acute respiratory distress syndrome (ARDS) is a systemic inflammation resulting from immune system overactivity. ARDS is also a fatal complication of COVID-19. Mesenchymal stem cells (MSCs) have immune modulatory properties. This study evaluated the safety and efficacy of three times transplantation of umbilical cord-derived MSCs (UC-MSCs) in terms of specific immunological and clinical changes in mild-to-moderate COVID-19-induced ARDS patients. In this single-center, open-label, phase 1 clinical trial, 20 patients diagnosed with COVID-19 and mild-to-moderate ARDS were included and were divided into two groups: a control group receiving standard care and an intervention group receiving UC-MSC in addition to standard care. Three consecutive intravenous transplants of UC-MSC (1× cells/kg body weight per each transplant) were performed in the intervention group on days 1, 3, and 5. The biological assay was investigated four times (days 0, 5, 10, and 17). UC-MSCs improved the patients' clinical and paraclinical parameters, including leukocytosis, lymphopenia, thrombocytopenia, and liver enzyme abnormalities compared to the control group. They also decreased pro-inflammatory lymphocytes (TH1 and TH17) and increased anti-inflammatory T lymphocytes. Cell therapy also reduced the mean fluorescence intensity (MFI) in overactivated CD8+ T cells. These findings show that three UC-MSC injections could regulate a hyperactivated immune system in COVID-19-induced ARDS patients by decreasing the inflammatory T lymphocyte subset and can improve the patient's hematological condition and liver function. However, more studies are needed in this area.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10533029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Soluble and Immobilized Anti-CD3/28 Distinctively Expand and Differentiate Primary Human T Cells: An Implication for Adoptive T Cell Therapy. 可溶性和固定化抗cd3 /28特异性扩增和分化原代人T细胞:对过继T细胞治疗的启示

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2022-12-24 DOI: 10.18502/ijaai.v21i6.11521

Tahereh Soltantoye, Behnia Akbari, Hamid Reza Mirzaei, Jamshid Hadjati

{"title":"Soluble and Immobilized Anti-CD3/28 Distinctively Expand and Differentiate Primary Human T Cells: An Implication for Adoptive T Cell Therapy.","authors":"Tahereh Soltantoye, Behnia Akbari, Hamid Reza Mirzaei, Jamshid Hadjati","doi":"10.18502/ijaai.v21i6.11521","DOIUrl":"https://doi.org/10.18502/ijaai.v21i6.11521","url":null,"abstract":"Cell-based cancer therapies have led to a paradigm shift in the treatment of patients with various cancers. To date, a vast majority of cancer immunotherapies have used genetically engineered T cells to target tumors. Stimulation and ex vivo expansion of T cells, as one of the crucial starting materials for T cell manufacturing, have always been a critical part of adoptive T-cell therapy (ACT). Typically, anti-CD3 and anti-CD28 monoclonal antibodies (mAbs) along with interleukin-2 (IL-2), through transducing signals one, two, and three, respectively, are essential for in vitro T cell activation. Terminal differentiation and replicative senescence are the main barriers of the ACTs during the manufacturing of engineered T cells ex vivo.In this study, we aimed to compare the T cell activation protocol that we developed in our lab (soluble anti-CD3/28 mAbs) with a common T cell activation protocol (immobilized anti-CD3/soluble anti-CD28) in terms of T cell expansion, activation, immunophenotype, and cellular fate. We observed that T cells were equally expanded in both protocols. Notably, our modified protocol promoted the outgrowth of CD8+ T cells postactivation. Concerning the low concentrations of both soluble anti-CD3 and anti-CD28, the modified protocol could significantly enrich memory T cell subsets. In conclusion, our data demonstrated that the soluble CD3/28 mAbs protocol is cost-effective and more efficient for generating more potent T cells, thereby expecting a better therapeutic outcome.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10544510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Placental Extract and Exosomes Derived from Pregnant Mice Attenuate the Development of Experimental Autoimmune Encephalomyelitis. 妊娠小鼠胎盘提取物和外泌体可减弱实验性自身免疫性脑脊髓炎的发展。

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2022-12-24 DOI: 10.18502/ijaai.v21i6.11525

Morteza Motallebnezhad, Shirin Taghizadeh, Tayebe Aghaie, Maryam Azimi, Ali-Akbar Salari, Mahmoud Bozorgmehr, Elahe Safari, Reza Falak, Mir-Hadi Jazayeri

{"title":"Placental Extract and Exosomes Derived from Pregnant Mice Attenuate the Development of Experimental Autoimmune Encephalomyelitis.","authors":"Morteza Motallebnezhad, Shirin Taghizadeh, Tayebe Aghaie, Maryam Azimi, Ali-Akbar Salari, Mahmoud Bozorgmehr, Elahe Safari, Reza Falak, Mir-Hadi Jazayeri","doi":"10.18502/ijaai.v21i6.11525","DOIUrl":"https://doi.org/10.18502/ijaai.v21i6.11525","url":null,"abstract":"Placental extract (PE) and exosomes from pregnant mice appear to have immunomodulatory and neuroprotective effects. In this study, we assessed the potential therapeutic effects of PE and exosomes obtained from pregnant mice in experimental autoimmune encephalomyelitis (EAE) mouse models. C57BL/6 mice, 8 to 12 weeks of age, were prepared and administered PE, exosomes, and glatiramer acetate (GA), as an FDA-approved treatment for multiple sclerosis (MS), after EAE induction. Thereafter, the therapeutic effects of treatment were evaluated by measuring the clinical courses of the mice as well as determining the number of regulatory T (Treg) cells using flow cytometry, cytokine levels, and microRNA-326 expression via real-time PCR. GA, PE, and exosomes reduced clinical severity, the extent of spinal cord demyelination, and the infiltration of inflammatory cells into the spinal cord. The frequency of CD4+CD25+FoxP3+ Treg cells increased after treatment of EAE mice with GA, PE, and exosomes. The mRNA expression of the inflammatory cytokines (interleukin-17 and interferon-gamma), as well as miR-326 expression, decreased significantly in the EAE mice after treatment with GA and exosomes. PE and exosomes from pregnant mice are involved in the modulation of Treg/Th17 balance and provide a therapeutic approach for MS. Further clinical studies will hopefully confirm the safety and efficacy of such treatments in MS patients.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10534597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Detection of Novel Autoantibodies to Nucleolin's RNA-binding Domains as a Serum Tumor Biomarker Through ELISA. 利用ELISA检测核仁蛋白rna结合域作为血清肿瘤生物标志物的新型自身抗体。

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2022-12-24 DOI: 10.18502/ijaai.v21i6.11520

Fatemeh Ezzatifar, Alireza Rafiei, Reza Valadan, Hossein Asgarian-Omran, Mahmood Jeddi-Tehrani

{"title":"Detection of Novel Autoantibodies to Nucleolin's RNA-binding Domains as a Serum Tumor Biomarker Through ELISA.","authors":"Fatemeh Ezzatifar, Alireza Rafiei, Reza Valadan, Hossein Asgarian-Omran, Mahmood Jeddi-Tehrani","doi":"10.18502/ijaai.v21i6.11520","DOIUrl":"https://doi.org/10.18502/ijaai.v21i6.11520","url":null,"abstract":"Expression and location of nucleolin are often abnormal in malignancies, which may result in the production of autoantibodies. Despite this, the identification of such autoantibodies may be essential for the early diagnosis and prognosis of cancers. In this investigation, the recombinant nucleolin protein was generated using an Escherichia coli expression system and was used an indirect enzyme-linked immunosorbent assay to detect anti-nucleolin autoantibodies in cancer patients' sera. Lung cancer patients' autoantibodies displayed the highest seroreactivity with the recombinant protein, with area under the curve of 0.948 and sensitivity and specificity of 85% and 96.67%, respectively (accuracy=92%). Anti-nucleolin autoantibodies were linked with lung tumor size (r=0.793), tumor, node, metastasis staging (r=0.643), and proliferation (r=0.744). These autoantibodies distinguished patients with early-stage lung cancer from healthy controls. Since anti-nucleolin autoantibodies are strongly linked to tumor size, clinical staging, and growth, they can be used to measure how well a treatment is working.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10534596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs Targeting Programmed Cell Death Protein 1 (PD-1) Promote Natural Killer Cell Exhaustion in Rheumatoid Arthritis. 靶向程序性细胞死亡蛋白1 (PD-1)的MicroRNAs促进类风湿关节炎的自然杀伤细胞衰竭。

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2022-12-24 DOI: 10.18502/ijaai.v21i6.11524

Maryam Hemmatzadeh, Elham Ahangar Parvin, Alireza Ghanavatinejad, Narges Rostami, Mehrzad Hajaliloo, Navid Shomali, Hamed Mohammadi, Farhad Jadidi-Niaragh

{"title":"MicroRNAs Targeting Programmed Cell Death Protein 1 (PD-1) Promote Natural Killer Cell Exhaustion in Rheumatoid Arthritis.","authors":"Maryam Hemmatzadeh, Elham Ahangar Parvin, Alireza Ghanavatinejad, Narges Rostami, Mehrzad Hajaliloo, Navid Shomali, Hamed Mohammadi, Farhad Jadidi-Niaragh","doi":"10.18502/ijaai.v21i6.11524","DOIUrl":"https://doi.org/10.18502/ijaai.v21i6.11524","url":null,"abstract":" Natural killer (NK) cells play a role in the pathogenesis of rheumatoid arthritis (RA). Upregulated levels of programmed cell death protein 1 (PD-1) is a sign of exhausted NK cells that could be regulated by microRNAs (miRNAs). In this investigation, we determined PD‑1 expression on NK cells (as a representation of NK cell exhaustion) in RA patients and evaluated if miRNAs are involved in the modulation of PD-1 expression in NK cells. Peripheral blood specimens were obtained from 40 RA patients and 20 healthy subjects. NK cells were isolated by negative selection from a pool of peripheral blood mononuclear cells. The frequency of PD-1-expressing NK cells and the expression of PD-1 on NK cells were analyzed by flow cytometry. Real-time PCR was used to measure the expression levels of PD-1 mRNA and miRNAs in the NK cells. The percentage of the PD-1-expressing NK cells and Mean fluorescence intensity (MFI) of PD-1 expression on the NK cells were significantly higher in the RA cases compared to the controls. The mRNA expression of PD-1 was significantly upregulated in NK cells from RA patients compared to healthy subjects. The expression levels of miR-28, miR-138, and miR-4717 were significantly downregulated in the NK cells from RA patients compared to the healthy group. In RA, miRNAs probably regulate the NK cell exhaustion process through driving PD-1 expression.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10539437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Importance of STAT3 Polymorphisms on the Risk and Clinical Characteristics of Rheumatoid Arthritis. STAT3基因多态性在类风湿关节炎发病风险及临床特征中的重要性

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2022-12-24 DOI: 10.18502/ijaai.v21i6.11523

Amirhossein Salehi, Ebrahim Hazrati, Hamta Ranjbar, Javad Behroozi, Bahram Pakzad, Maryam Mousavi, Mojtaba Mousavi, Marzieh Hossein Balam, Mansour Salesi

{"title":"Importance of STAT3 Polymorphisms on the Risk and Clinical Characteristics of Rheumatoid Arthritis.","authors":"Amirhossein Salehi, Ebrahim Hazrati, Hamta Ranjbar, Javad Behroozi, Bahram Pakzad, Maryam Mousavi, Mojtaba Mousavi, Marzieh Hossein Balam, Mansour Salesi","doi":"10.18502/ijaai.v21i6.11523","DOIUrl":"https://doi.org/10.18502/ijaai.v21i6.11523","url":null,"abstract":"Signal transducer and activator of transcription 3 (STAT3) has been introduced as one of the critical genetic factors in the pathogenesis of rheumatoid arthritis (RA). Single nucleotide polymorphisms (SNPs) in microRNA binding sites, known as miRSNPs, are a class of common variants in the 3' untranslated regions of genes targeted by miRNAs. miRSNPs unbalance gene expression by disrupting the binding regions of microRNAs. In this study, we intended to evaluate the association of two miRSNPs with the risk of RA development and its clinical features. We studied 120 Iranian patients with RA and 125 non-RA subjects as controls. The genotypes and alleles of rs1053005 and rs1053023 in each individual were assessed by the high-resolution melting method. The distribution of STAT3 variants did not differ markedly in RA patients compared to healthy controls. Stratification analysis revealed that rs1053005 was linked with a higher concentration of C-reactive protein and an increased erythrocyte sedimentation rate, two indicators of inflammation and disease activity in RA patients. The rs1053023 variant was correlated with higher levels of creatinine as an indicator of renal involvement. Our data demonstrate an association between STAT3 variants and clinical characteristics of RA, such as disease activity and probably kidney impairment. However, we did not observe a significant relationship between the two targeted variants and a predisposition to RA.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10539436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

β-D-mannuronic Acid (M2000) and Inflammatory Cytokines in COVID-19; An In vitro Study. β- d -甘露醛酸(M2000)与COVID-19炎症因子的关系体外研究。

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2022-12-24 DOI: 10.18502/ijaai.v21i6.11528

Behrouz Robat-Jazi, Khodayar Ghorban, Mohammad Gholami, Esmaeil Samizadeh, Zahra Aghazadeh, Mohammad Amin Shahrbaf, Maryam Dadmanesh, Negin Hosseini Rouzbahani, Abbas Mirshafiey

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引用次数: 2