Iranian journal of allergy, asthma, and immunology最新文献_第2页

Measurement of the Neutrophils Count and Oxidative Burst in Neutrophils of Patients with Sanjad Sakati Syndrome. 桑贾德-萨卡提综合征患者中性粒细胞计数和氧化爆发的测定

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2024-02-11 DOI: 10.18502/ijaai.v23i1.14959

Farhad Abolnezhadian, Majid Aminzadeh, Sara Iranparast, Sajad Dehnavi, Fatemeh Dousti, Moosa Sharifat, Hamid Moradzadegan

{"title":"Measurement of the Neutrophils Count and Oxidative Burst in Neutrophils of Patients with Sanjad Sakati Syndrome.","authors":"Farhad Abolnezhadian, Majid Aminzadeh, Sara Iranparast, Sajad Dehnavi, Fatemeh Dousti, Moosa Sharifat, Hamid Moradzadegan","doi":"10.18502/ijaai.v23i1.14959","DOIUrl":"10.18502/ijaai.v23i1.14959","url":null,"abstract":"Sanjad Sakati Syndrome (SSS) is categorized as a neuroendocrine-related disease due to disorders of the nervous and hormonal systems. Since hormonal changes in these patients may affect the nature and function of the immune system. Thus, in this study, cell count and phagocytotic function of neutrophils were evaluated which may be influenced by changes in the hormonal rate and growth factors. In this study, the neutrophil count value and the oxidative burst were evaluated in six patients diagnosed with SSS and six healthy individuals. There was a significant reduction in the neutrophil count observed in SSS patients compared to healthy controls (37.41±7.93 percent vs. 66.5±6.8 percent). However, there was no significant difference in neutrophil oxidative index between patients with SSS and control subjects (172.33±55.08 vs. 217.00±77.38). We concluded that in patients with SSS, the phagocytic activity of neutrophils was not affected by hormonal changes, while the number of neutrophils and neutrophil-to-lymphocyte ratio (NLR) index were decreased.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Innate and Adaptive Immune System in the Pathogenesis of Schizophrenia. 先天性和适应性免疫系统在精神分裂症发病机制中的作用。

IF 1.5 4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2024-02-11 DOI: 10.18502/ijaai.v23i1.14951

Marziyeh Soltani, Yousef Mirzaei, Ali Hussein Mer, Mina Mohammad-Rezaei, Zahra Shafaghat, Soheila Fattahi, Fatemeh Azadegan-Dehkordi, Meghdad Abdollahpour-Alitappeh, Nader Bagheri

{"title":"The Role of Innate and Adaptive Immune System in the Pathogenesis of Schizophrenia.","authors":"Marziyeh Soltani, Yousef Mirzaei, Ali Hussein Mer, Mina Mohammad-Rezaei, Zahra Shafaghat, Soheila Fattahi, Fatemeh Azadegan-Dehkordi, Meghdad Abdollahpour-Alitappeh, Nader Bagheri","doi":"10.18502/ijaai.v23i1.14951","DOIUrl":"10.18502/ijaai.v23i1.14951","url":null,"abstract":"Schizophrenia is one of the most severely debilitating mental disorders that affects 1.1% of the world's population. The exact cause of the disease is not known, but genetics, environmental factors (such as infectious agents, season and region of birth, exposure to viruses, low birth weight, advanced paternal age, and tobacco), and immune system dysfunction can all contribute to the development of schizophrenia. Recently, the role of the immune system in schizophrenia has received much attention. Both acquired and innate immune systems are involved in the pathogenesis of schizophrenia and facilitate the disease's progression. Almost all cells of the immune system including microglia, B cells, and T cells play an important role in the blood-brain barrier damage, inflammation, and in the progression of this disease. In schizophrenia, the integrity of the blood-brain barrier is reduced and then the immune cells are recruited into the endothelium following an increase in the expression of cell adhesion molecules. The entry of immune cells and cytokines leads to inflammation and antibody production in the brain. Accordingly, the results of this study strengthen the hypothesis that the innate and acquired immune systems are involved in the pathogenesis of schizophrenia.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory Effect of Calcitriol on Experimental Autoimmune Encephalomyelitis Mice, A Multiple Sclerosis Animal Model 骨化三醇对实验性自身免疫性脑脊髓炎小鼠(多发性硬化症动物模型)的免疫调节作用

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13995

Behrouz Robat-Jazi, Mona Oraei, Sama Bitarafan, Seyed Alireza Mesbah-Namin, Ali Noori-Zadeh, Fatemeh Mansouri, Karim Parastouei, Ali Anissian, Mir Saeed Yekaninejad, Ali Akbar Saboor-Yaraghi

{"title":"Immunomodulatory Effect of Calcitriol on Experimental Autoimmune Encephalomyelitis Mice, A Multiple Sclerosis Animal Model","authors":"Behrouz Robat-Jazi, Mona Oraei, Sama Bitarafan, Seyed Alireza Mesbah-Namin, Ali Noori-Zadeh, Fatemeh Mansouri, Karim Parastouei, Ali Anissian, Mir Saeed Yekaninejad, Ali Akbar Saboor-Yaraghi","doi":"10.18502/ijaai.v22i5.13995","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13995","url":null,"abstract":"Previous studies noted an imbalance in T helper (Th) 17 and regulatory T cells (Tregs) in experimental autoimmune encephalomyelitis (EAE), a multiple sclerosis animal model. calcitriol, vitamin D's active form, was found to ameliorate EAE symptoms by favoring Tregss over Th17 cells, suggesting immunomodulatory effects. This study aimed to assess calcitriol's impact on EAE manifestations and cytokine profile in mice. In this study, we recruited twenty-eight C57BL/6 mice and divided them into 4 groups: healthy controls, EAE, EAE with calcitriol treatment, and healthy mice with calcitriol treatment. CD4+ T cells were isolated from splenocytes using magnetic-activated cell sorting. Real-time polymerase chain reaction was employed to quantify the genes associated with Th9 cells (i.e., SPI1 encoding PU.1 and IL9 encoding interleukin [IL]-9). Moreover, the levels of IL-17 and transforming growth factor beta (TGF-β) were evaluated through enzyme-linked immunosorbent assay in the supernatant of CD4+ T cell culture stimulated by anti-CD3 and anti-CD28 antibodies for 72 hours. In the supernatant of CD4+ T cell cultures, the levels of interleukin-17 (IL-17) were significantly increased, while the levels of transforming growth factor beta (TGF-β) were decreased in the EAE Group compared to the healthy control group. Calcitriol treatment reversed these changes and attenuated EAE symptoms, as confirmed in hematoxylin and eosin, and luxol fast blue stains. Notably, calcitriol increased IL9 gene expression in both EAE and healthy mice. This study provides further evidence of the anti-inflammatory effects of calcitriol and its role in attenuating EAE.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135475684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amygdalin Improves Allergic Asthma via the Thymic Stromal Lymphopoietin–dendritic Cell–OX40 Ligand Axis in a Mouse Model 小鼠模型中苦杏仁苷通过胸腺基质淋巴生成素-树突状细胞ox40配体轴改善过敏性哮喘

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13993

Wen Cui, Huan Zhou, Ya-zun Liu, Yan Yang, Yi-zhong Hu, Zhao-peng Han, Jian-er Yu, Zheng Xue

{"title":"Amygdalin Improves Allergic Asthma via the Thymic Stromal Lymphopoietin–dendritic Cell–OX40 Ligand Axis in a Mouse Model","authors":"Wen Cui, Huan Zhou, Ya-zun Liu, Yan Yang, Yi-zhong Hu, Zhao-peng Han, Jian-er Yu, Zheng Xue","doi":"10.18502/ijaai.v22i5.13993","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13993","url":null,"abstract":"Asthma, characterized by persistent inflammation and increased sensitivity of the airway, is the most common chronic condition among children. Novel, safe, and reliable treatment strategies are the focus of current research on pediatric asthma. Amygdalin, mainly present in bitter almonds, has anti-inflammatory and immunoregulatory potential, but its effect on asthma remains uninvestigated. Here, the impact of amygdalin on the thymic stromal lymphopoietin (TSLP)–dendritic cell (DC)–OX40L axis was investigated. A BALB/c mouse model for allergic asthma was established using the ovalbumin-sensitization method. Amygdalin treatment was administered between days 21 and 27 of the protocol. Cell numbers and hematoxylin and eosin (H&E) staining in bronchoalveolar lavage fluid (BALF) were used to observe the impact of amygdalin on airway inflammation. TSLP, IL-4, IL-5, IL-13, and IFN-γ concentrations were determined via Enzyme-linked immunosorbent assay (ELISA). TSLP, GATA-3, and T-bet proteins were measured using western blotting. Cell-surface receptor expression on DCs (MHC II, CD80, and CD86) was assessed via flow cytometry. OX40L mRNA and protein levels were detected using western blotting and qRT-PCR, respectively. Amygdalin treatment attenuated airway inflammation decreased BALF TSLP levels, inhibited DC maturation, restrained TSLP-induced DC surface marker expression (MHCII, CD80, and CD86), and further decreased OX40L levels in activated DCs. This occurred together with decreased Th2 cytokine levels (IL-4, IL-5, and IL-13) and GATA3 expression, whereas Th1 cytokine (IFN-γ) levels and T-bet expression increased. Amygdalin thus regulates the Th1/Th2 balance through the TSLP–DC–OX40L axis to participate in inflammation development in the airways, providing a basis for potential allergic asthma treatments.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135475685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined Treatment of Progressive Encephalitis in an X-linked Agammaglobulinemia Patient x连锁无球蛋白血症患者进行性脑炎的综合治疗

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13999

Marco Antonio Yamazaki-Nakashimada, Patricia Herrera-Mora, Alfonso Mahrx-Bracho, Gabriela López-Herrera, Juan Carlos Bustamante-Ogando, Selma Cecilia Scheffler-Mendoza

引用次数: 0

MicroRNA-29a-3p Accelerates Inflammatory Damage in Neonatal Pneumonia Via Targeting Krüppel-like Factor 4 MicroRNA-29a-3p通过靶向kr<s:1> ppel样因子4加速新生儿肺炎的炎症损伤

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13994

Xiao Juan Xu, Wei Liu, ShiNa Liland

{"title":"MicroRNA-29a-3p Accelerates Inflammatory Damage in Neonatal Pneumonia Via Targeting Krüppel-like Factor 4","authors":"Xiao Juan Xu, Wei Liu, ShiNa Liland","doi":"10.18502/ijaai.v22i5.13994","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13994","url":null,"abstract":"Neonatal pneumonia (NP) is a frequently occurring illness during the neonatal phase. The study investigated the molecular process and the role of microRNA (miR)-29a-3p in NP. Peripheral blood was collected from NP patients and healthy newborns. Human lung fibroblasts cell line (WI-38) were treated with lipopolysaccharide (LPS)) to establish a cellular model for NP. Then, miR-29a-3p and Krüppel-like Factor 4 (KLF4) levels were detected by RT-qPCR or Western blot. The relationship between miR-29a-3p and KLF4 was confirmed by dual luciferase reporter gene assay. Cell survival was assessed using the CCK-8 assay, whereas the levels of interleukin-6, tumor necrosis factor-α, and IL-1β were quantified using ELISA. Additionally, apoptosis was evaluated through flow cytometry. Meanwhile, Bax and Bcl-2 were detected by RT-qPCR. Neonatal rats were administered LPS intraperitoneally (3 mg/kg) to induce NP, and pathological injury and inflammatory reaction were analyzed. MiR-29a-3p was elevated but KLF4 was silenced in NP patient’s serum, LPS-treated WI-38 cell line, and LPS-treated newborn rats. Silence of miR-29a-3p or elevation of KLF4 constrained cell proliferation with inflammation of LPS-treated WI-38 cell line. MiR-29a-3p immediately targeted KLF4. Additionally, silence of miR-29a-3p alleviated LPS-stimulated lung injury and inflammation in neonatal rats. The protective action of silenced miR-29a-3p in LPS-treated WI-38 cell line and newborn rats was turned around by silencing KLF4. This study demonstrates originally that miR-29a-3p boosts inflammatory damage in NP via targeting KLF4, offering a basis for clinically diagnosing and treating NP.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135476706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cycle Threshold Values Predict COVID-19 Severity and Mortality but are not Correlated with Laboratory Markers 周期阈值预测COVID-19严重程度和死亡率，但与实验室标志物无关

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13996

Behnaz Esmaeili, Hoda Khoshnevis, Atefe Alirezaee, Abbas Shakoori, Zahra Pourpak, Hamid Chegini, Zahra Ahmadinejad

{"title":"Cycle Threshold Values Predict COVID-19 Severity and Mortality but are not Correlated with Laboratory Markers","authors":"Behnaz Esmaeili, Hoda Khoshnevis, Atefe Alirezaee, Abbas Shakoori, Zahra Pourpak, Hamid Chegini, Zahra Ahmadinejad","doi":"10.18502/ijaai.v22i5.13996","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13996","url":null,"abstract":"Many studies have evaluated the possible utility of cycle threshold (Ct) values as a predictor of Coronavirus disease 2019 (COVID-19) severity and patient outcome. Given the inconsistent results, we aimed to evaluate the association between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Ct values and disease severity, inflammatory markers, and outcomes in Iranian patients with COVID-19. A retrospective study of 528 patients with COVID-19 hospitalized from September 2020 to October 2021 was conducted. Demographic, clinical, and laboratory data of patients were retrieved from electronic medical records. Ct values were analyzed as a continuous variable after subcategorizing into 3 groups: low (Ct values<20), medium (Ct values 20 to 30), and high (Ct values>30). Of the 528 patients (45.1% female) aged 13 to 97 years, 109 patients had low Ct values, 312 patients had medium, and 107 patients had high Ct values. Patients with low Ct values were more likely to present with critical COVID-19, require invasive mechanical ventilation and develop complications such as acute respiratory distress syndrome and pneumonia. Furthermore, patients with low or medium Ct values were more likely to die compared to patients with high Ct values.Multivariate analysis showed that patients with low or medium Ct values were more likely to have severe COVID-19 compared with patients with high Ct values. The multivariate analysis also showed a higher risk of mortality in patients with low Ct values compared to patients with high Ct values, although this was not statistically significant. Our findings revealed that Ct values were an independent predictor of COVID-19 severity. The risk of mortality was higher in patients with low Ct values. However, further investigation is needed to address the correlation between Ct values and inflammatory factors.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135476702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Analysis of Long Noncoding RNA-MALAT1 and Interleukin-6 in Inflammatory Bowel Disease Patients 长链非编码RNA-MALAT1和白细胞介素-6在炎症性肠病患者中的表达分析

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13997

Mohsen Nemati Bajestan, Moein Piroozkhah, Vahid Chaleshi, Naser Elmi Ghiasi, Negar Jamshidi, Reza Mirfakhraie, Hedieh Balaii, Shabnam Shahrokh, Hamid Asadzadeh Aghdaei, Zahra Salehi, Ehsan Nazemalhosseini Mojarad

{"title":"Expression Analysis of Long Noncoding RNA-MALAT1 and Interleukin-6 in Inflammatory Bowel Disease Patients","authors":"Mohsen Nemati Bajestan, Moein Piroozkhah, Vahid Chaleshi, Naser Elmi Ghiasi, Negar Jamshidi, Reza Mirfakhraie, Hedieh Balaii, Shabnam Shahrokh, Hamid Asadzadeh Aghdaei, Zahra Salehi, Ehsan Nazemalhosseini Mojarad","doi":"10.18502/ijaai.v22i5.13997","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13997","url":null,"abstract":"Inflammatory bowel disease (IBD) manifests as chronic inflammation within the gastrointestinal tract. The study focuses on a long noncoding RNA (lncRNA) known as Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1). MALAT1's misregulation has been linked with various autoimmune diseases and regulates proinflammatory cytokines. The role of IL6 in immune-triggered conditions, including IBD, is another focal point. In this research, the expression of MALAT1 and IL6 in IBD patients was meticulously analyzed to uncover potential interactions. The study involved 33 IBD patients (13 with Crohn's disease and 20 with ulcerative colitis) and 20 healthy counterparts. Quantitative real-time polymerase chain reaction determined the MALAT1 and IL6 gene expression levels. The competitive endogenous RNA (ceRNA) regulatory network was constructed using several tools, including LncRRIsearch and Cytoscape. A deep dive into the Inflammatory Bowel Disease database was undertaken to understand IL6's role in IBD. Drugs potentially targeting these genes were also pinpointed using DGIdb. Results indicated a notable elevation in the expression levels of MALAT1 and IL6 in IBD patients versus healthy controls. MALAT1 and IL6 did not show a direct linear correlation, but IL6 could serve as MALAT1's target. Analyses unveiled interactions between MALAT1 and IL6, regulated by hsa-miR-202-3p, hsa-miR-1-3p, and has-miR-9-5p. IL6's pivotal role in IBD-associated inflammation, likely interacting with other cytokines, was accentuated. Moreover, potential drugs like CILOBRADINE for MALAT1 and SILTUXIMAB for IL6 were identified. This research underscored MALAT1 and IL6's potential value as targets in diagnosis and treatment for IBD patients.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135475687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic Allergen Exposure Contributes to Steroid Resistance via Increased Phosphorylation of Glucocorticoid Receptors S226 and p38 MAPK in a Mouse Model of Asthma 在哮喘小鼠模型中，慢性过敏原暴露通过糖皮质激素受体S226和p38 MAPK磷酸化的增加有助于类固醇抵抗

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13992

Yan Zhou, Limin Wang, Weizhong Jin, Chenhui Qiu, Hualiang Jin

{"title":"Chronic Allergen Exposure Contributes to Steroid Resistance via Increased Phosphorylation of Glucocorticoid Receptors S226 and p38 MAPK in a Mouse Model of Asthma","authors":"Yan Zhou, Limin Wang, Weizhong Jin, Chenhui Qiu, Hualiang Jin","doi":"10.18502/ijaai.v22i5.13992","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13992","url":null,"abstract":"Chronic allergen exposure can significantly induce p38 mitogen-activated protein kinase (MAPK) activation in asthma. p38 MAPK is involved in steroid resistance through phosphorylation of glucocorticoid receptors (GR) at S226. This study aims to investigate whether chronic allergen exposure can induce steroid resistance and whether it is associated with p38 MAPK activation in asthma. A mouse model of asthma was prepared by sensitizing and challenging mice with chronic ovalbumin (OVA) exposure. Key features of allergic asthma, encompassing bronchial hyperresponsiveness, pathology of lung tissues, cytokine profiles of inflammation in bronchoalveolar lavage fluid (BALF), and serum immunoglobulin (Ig)E concentration were evaluated. Furthermore, suppressive effects of corticosteroid on the splenocytes under stimulation of lipopolysaccharides, glucocorticoid receptor (GR) DNA binding ability of splenocytes, expression of GRα and phosphorylation of GR s226 in splenocytes, and p38 MAPK phosphorylation in splenocytes and lung tissues were determined. Chronic OVA exposure substantially induced airway hypersensitivity, leading to increased inflammatory infiltration in lung tissues. Additionally, it resulted in elevated levels of interleukin (IL)-4, IL-5, and IL-6 in BALF, as well as heightened levels of IgE in serum. Furthermore, OVA exposure substantially enhanced p38 MAPK phosphorylation in lung tissues. It also weakened the suppressive impacts of corticosteroids on splenocytes, impaired the GR DNA binding ability, and led to an enhanced phosphorylated state of GR S226 and p38 MAPK in splenocytes. Taken together, chronic allergen exposure contributes to steroid resistance in asthma, which is linked to an increased phosphorylated state of GR S226 and p38 MAPK.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135476700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Oral Montelukast in Controlling Asthma Attacks in Children; A Randomized Double-blind Placebo Control Study 口服孟鲁司特对儿童哮喘发作的控制作用随机双盲安慰剂对照研究

4区医学

Iranian journal of allergy, asthma, and immunology Pub Date : 2023-11-07 DOI: 10.18502/ijaai.v22i5.13990

Mohsen Jafari, Masoomeh Sobhani, Kambiz Eftekhari, Armen Malekiantaghi, Mohammad Gharagozlou, Alireza Shafiei

{"title":"The Effect of Oral Montelukast in Controlling Asthma Attacks in Children; A Randomized Double-blind Placebo Control Study","authors":"Mohsen Jafari, Masoomeh Sobhani, Kambiz Eftekhari, Armen Malekiantaghi, Mohammad Gharagozlou, Alireza Shafiei","doi":"10.18502/ijaai.v22i5.13990","DOIUrl":"https://doi.org/10.18502/ijaai.v22i5.13990","url":null,"abstract":"Oral Montelukast is recommended as maintenance therapy for persistent asthma, but there is controversy regarding its effectiveness in controlling asthma attacks. The present study was conducted to investigate the clinical efficacy of oral Montelukast for asthma attacks in children. This study was conducted as a double-blind placebo-controlled clinical trial on 80 children aged 1-14 years with asthma who were admitted to the emergency department of Bahrami Children's Hospital (Tehran, Iran) during one year. Patients were randomly divided into case and control groups. In addition to the standard asthma attack treatment, Montelukast was prescribed in the case group and placebo in the control group for one week. Patients were evaluated in terms of asthma attack severity score and oxygen saturation percentage (SpO2) in room air as primary outcomes 1, 4, 8, 24 and 48 hours after admission. In the first 48 hours, there was no significant difference in the score of asthma attack severity and SpO2 between the case and control groups. There was no significant difference between the groups in terms of length of hospitalization or number of admissions to the intensive care unit. None of the patients were re-hospitalized after discharge. The results of this study showed that the use of Montelukast along with the standard treatment of asthma attacks in children has no added benefit.","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135476560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0