{"title":"Plant growth and total flavonoid content of Sisyrinchium palmifolium after light intensity and gibberellin treatment","authors":"Puji Astuti Kwaswantari Zuaini, Widya Mudyantini, Solichatun Solichatun","doi":"10.13057/cellbioldev/v040203","DOIUrl":"https://doi.org/10.13057/cellbioldev/v040203","url":null,"abstract":"Abstract. Zuaini PAK, Mudyantini W, Solochatun. 2020. Plant growth and total flavonoid content of Sisyrinchium palmifolium after light intensity and gibberellin treatment. Cell Biol Dev 4: 52-63. Dayak onion (Sisyrinchium palmifolium L Syn.: Eleutherine palmifolia L. Merr.) was a plant that has the potential to be developed as a traditional medicine because it contains flavonoid compounds. This research aimed to determine the effect of the application of differences in light intensity and gibberellins on the plant growth and flavonoid content of S. palmifolium. This research used uniform S. palmifolium with a harvest age of 3-4 months and an 8-9 g weight range from Pasir Besar Village, South Pontianak District, Pontianak, Indonesia. The experiment used the Factorial Completely Randomized Design method with two factors treatment and six replications: light intensity (100%, 75%, 50%) and gibberellin concentration (0, 10, 20 ppm). The control was 100% light intensity treatment and 0 ppm gibberellin concentration. The parameters measured were the number of leaves, length, width, time of flowering, number of flowers, and number of bulbs. In the post-harvest, dry and wet weights of bulbs were measured. Then, chlorophyll, carotenoid, and flavonoid contents were assessed using a UV-Vis spectrophotometer, and stomata density was also analyzed. ANOVA analyzed the data, and if the difference was significant, it was continued with the DMRT test at a significance level of 5%. The results showed that the growth of S. palmifolium was significantly affected by light intensity and gibberellins on the parameters of leaves length; leaves, midrib, and bulbs wet weight; leaves and midrib wet weight; leaves and midrib dry weight; bulbs dry weight; shoot root ratio; leaves carotenoid content, and bulbs carotenoid content. However, parameters had no significant effect on the number of leaves, leaves width, time of flowering, number of flowers, number of bulbs, chlorophyll contents, flavonoid, and stomata density. The 50% light intensity and 10 ppm gibberellins were the best treatments to increase leaves length, leaves, midrib, bulbs wet weight, leaves, and bulbs chlorophyll content of S. palmifolium.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"352 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133134569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of mineral levels and leaf extracts of some plants on soil pH and growth rate of selected non-leguminous plants","authors":"Mwangi James Njogu, J. Murungi, R. Wanjau","doi":"10.13057/cellbioldev/v050204","DOIUrl":"https://doi.org/10.13057/cellbioldev/v050204","url":null,"abstract":"Abstract. Njogu MJ, Murungi JI, Wanjau RN. 2021. Effects of mineral levels and leaf extracts of some plants on soil pH and growth rate of selected non-leguminous plants. Cell Biol Dev 5: 76-89. The purpose of this study was to determine the effect of growth rate on non-leguminous plants such as wheat (Triticum aestivum L.), kale (Brassica oleracea var acephala), and coriander (Coriandrum sativum L.) grown on acidic soils treated with leaves and leaf extracts of plants known to have a high buffering capacity and mineralization capacity, i.e., Jacaranda mimosifolia D. Don, Cordia africana Lam, Croton macrostachyus Hochst. ex Delile, Vitex keniensis Turril, Tithonia diversifolia (Hemsl.) A.Gray, Manihot esculenta Crantz, Carica papaya L., and Helianthus annuus L. For this investigation, the leaves and leaf extracts were applied to the soil with a pH of 4.75. Leaf extracts and leaves of eight species were combined according to the macronutrient content and mineralization of chosen plants discovered in Nyandarua County, Kenya. Wheat, kale, and coriander growth rates were determined by comparing the dry mass of uprooted seedlings from the trial and control studies every fourteen days for 60 days. Flame photometry, atomic absorption spectrometry (AAS), UV/visible spectroscopy, and turbidimetry were used to determine macronutrients in leaf extracts. The analysis of variance (ANOVA) and Student-Newman-Keuls (SNK) tests were used to analyze the data. For 60 days, the mean pH value of leaf extracts ranged from 4.580.27a to 7.440.04d. K+; 189.95±0.17c, Ca2+; 367.33±0.67e, Mg2+; 114.33e±0.33, PO43-; 55.38±0.23f, NO3-; 322.25±0.40f, and SO42-; 56.48±0.23e had the greatest mean levels in leaves (?g/g). Macronutrient levels were significantly different in soil treated with leaves and leaf extract (Le), commercial NPK fertilizers (Cf), and untreated soil (Us). Wheat (dry mass) grew at a mean rate of 1.27±0.13b g/wk (Le), 1.26±0.12b g/wk (Cf) and 0.32±0.02a g/wk (Us), while in (height) was 7.29±1.43b cm/wk (Le), 6.20±1.95b cm/wk (Cf), 3.98±0.97a cm/wk (Us). For kales 0.20±0.01a g/wk (Le), 0.30±0.02a g/wk (Cf), and 0.03±0.01b g/k (Us), while in (height) 2.15±0.85c cm/wk (Le), 2.57±0.88b cm/wk (Cf), and 1.04±0.02a cm/wk (Us). The mean growth rate for corianders was 0.16±0.01a g/wk (Le), 0.17±0.02a g/wk (Cf), and 0.10±0.01a g/k (Us), while in (height) 1.85±0.56b cm/wk (Le), 1.86±0.58b cm/wk (Cf) and 0.79±0.18a cm/wk (Us). This study's findings show that the plant's leaves and leaf extracts can be used in place of inorganic fertilizers and should be recommended for agroforestry in low-pH environments.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"225 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115739116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Setianingrum Andaryani, Samanhudi Samanhudi, A. Yunus
{"title":"Effect of BAP and 2,4-D on callus induction of Jatropha curcas in vitro","authors":"Setianingrum Andaryani, Samanhudi Samanhudi, A. Yunus","doi":"10.13057/cellbioldev/v030202","DOIUrl":"https://doi.org/10.13057/cellbioldev/v030202","url":null,"abstract":"Abstract. Andaryani S, Samanhudi, Yunus A. 2019. Effect of BAP and 2,4-D on callus induction of Jatropha curcas in vitro. Cell Biol Dev 3: 56-65. The aim of this study was to obtain appropriate concentrations of Benzyl Amino Purine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) to increase callus induction in vitro using shoot explants of Jatropha curcas L. The research was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia, from July to August 2010. The study used a completely randomized design (CRD) with two treatment factors and three replications. The first factor is the level of BAP concentration, namely: 0.5 ppm, 1 ppm, 1.5 ppm, and 2 ppm. The second factor is the concentration of 2,4-D, namely: 0 ppm, 0.25 ppm, 0.5 ppm, and 0.75 ppm. Observation variables included callus emergence time, callus color, callus texture, root emergence time, number of roots, shoot emergence time, number of shoots, leaf emergence time, number of leaves, and callus fresh weight. Qualitative data are presented descriptively. Quantitative data includes callus fresh weight data, which was analyzed for variance based on the 5% F test and continued with the DMRT test at the 5% level. The results showed that the combination of 2 ppm BAP treatment and 0.25 ppm 2,4-D was the fastest in inducing callus, 5.67 Days After Planting (DAP). All the resulting calluses have a crumb texture. The average callus color in all treatment combinations was yellowish-green. Only the treatment combination of 1 ppm BAP without 2,4-D could produce roots at 9 DAP. The combination of 0.5 ppm BAP treatment and 0.25 ppm 2,4-D was the fastest to produce shoots, 6 DAP. The fastest leaf emergence was obtained in the 1 ppm BAP treatment without 2,4-D, 11 DAP. The combination of 2 ppm BAP treatment and 0.5 ppm 2,4-D resulted in the largest callus fresh weight (2.56 g).","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123905425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physiological and phytochemical characters of Eleutherine palmifolia affected by treatment of variation in light intensity and water capacity","authors":"Naufal Firdaus, Widya Mudyantini, S. Sugiyarto","doi":"10.13057/cellbioldev/v040103","DOIUrl":"https://doi.org/10.13057/cellbioldev/v040103","url":null,"abstract":"Abstract. Firdaus NM, Mudyantini W, Sugiarto. 2020. Physiological and phytochemical characters of Eleutherine palmifolia affected by treatment of variation in light intensity and water capacity. Cell Biol Dev 4: 26-39. Bawang dayak (Eleutherine palmifolia (L.) Merr.) is one of the tubers widely cultivated in Kalimantan with phytopharmacological potential, but its usage in traditional medicine is still limited. This study aims to ascertain the physiological and phytochemical characteristics of E. palmifolia following treatment with varying amounts of water and light intensity. The study used a completely randomized design (CRD) with two components: the treatment of light intensity 50% and 100% and water availability at 50%, 75%, and 100% concentrations, to create six treatment combinations. Flowering pace, leaf number, breadth, length, and blossom number were measured once every three days for one month. Wet weight, dry weight, stomata index, chlorophyll, carotene, vitamin C, flavonoids, and shoot ratio were assessed at harvest. The data were analyzed using ANOVA (Analysis of Variant). If there was a significant difference, a further test was carried out with the Duncan Multiple Range Test with a test level of 5%. The treatment effect on water availability of 75% and light intensity of 50% (K75I50) was significantly different and gave the highest value on leaf width of 0.28 cm, stomata index of 36.84 cm, carotenoid content of 39.70 g/ mL in E. palmifolia. The given treatments were not significantly different in the number of leaves, leaf length, the number of flowers, wet weight, dry weight, chlorophyll, vitamin C, flavonoids, and shoot ratio of Dayak roots. Water availability and 100% light intensity (K100I100) are the optimal treatments to accelerate the flowering of Dayak onion plants with a yield of 26.00 days.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126709993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayu Sulistiyaning Utami, M. Harini, Nor Soesanti Handajani, Lutfi Nur Hidayat
{"title":"Effect of carrot leaf juice (Daucus carota) on the lung histology structure of white rats (Rattus norvegicus) induced by cigarette smoke","authors":"Ayu Sulistiyaning Utami, M. Harini, Nor Soesanti Handajani, Lutfi Nur Hidayat","doi":"10.13057/cellbioldev/v040104","DOIUrl":"https://doi.org/10.13057/cellbioldev/v040104","url":null,"abstract":"Abstract. Utami AS, Harini M, Handajani NS, Hidayat LN. 2020. Effect of carrot leaf juice (Daucus carota) on the lung histology structure of white rats (Rattus norvegicus) induced by cigarette smoke. Cell Biol Dev 4: 40-45. Cigarette smoke is hazardous to both active and passive smokers. The impact results in oxidative stress, which results in cell damage across the lung's histological structure, including tissue dilation in the alveoli, epithelial abrasion, and other abnormalities. Carrot leaves contain beta carotene, an antioxidant that can help repair damaged cells caused by cigarette smoke's oxidative stress. The goal of this study was to assess the histological structure of the lungs following induction with cigarette smoke and the influence of carrot leaf juice (Daucus carota L.) on the lung structure following induction with cigarette smoke. A completely randomized design (CRD) with five replications was adopted in this investigation. The rats were divided into four groups: those induced by cigarette smoke and given 4 mL distilled water, those induced by cigarette smoke and given 4 mL carrot leaf juice (2 mL carrot leaf juice added to 2 mL distilled water), those induced by cigarette smoke and given 4 mL carrot leaf juice (3 mL carrot leaf juice added to 1 mL distilled water), and those induced by cigarette smoke and given 4 mL oat straw juice. For 14 days, white rats were provoked with three cigarettes every day. On the 31st day, rats were killed via cervical dislocation, their lungs were removed, and histological preparations using the paraffin method and Hematoxylin-Eosin staining were performed. Quantitative data were analyzed using non-parametric techniques Anova test, and qualitative data collection by describing the effect of cigarette smoke before and after carrot leaf juice administration.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"2022 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114489988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of the media type and auxin concentration on the growth of cuttings seedlings of pepper (Piper nigrum)","authors":"S. Amanah, M. Budiastuti, Ato Sulistyo","doi":"10.13057/cellbioldev/v060105","DOIUrl":"https://doi.org/10.13057/cellbioldev/v060105","url":null,"abstract":"Abstract. Amanah S, Budiastuti MTHS, Sulistyo A. 2022. Effect of the media type and auxin concentration on the growth of cuttings seedlings of pepper (Piper nigrum). Cell Biol Dev 6: 32-40. Pepper (Piper nigrum L.) is an export commodity and has become one of the Indonesian sources of income. It is necessary to develop the best cultivation for increasing pepper production. Vegetative propagation has been chosen in this research, using manure as part of the media and auxin as a growth hormone. The research aims to find the best media and auxin concentration for the ideal growth of pepper grafting. The research conducted in Manyaran Wonogiri, Indonesia, with RCB design consists of two factors arranged in the factorial method. The first factor is the kind of media (soil, soil + manure, soil + manure + husk), and the second is auxin's concentration (0 g/L, 12.5 g/L, 25 g/L, 37.5 g/L; 50 g/L). Observation variables are bud growth time, number of buds, length of bud, number of leaves, leaf width, number of roots, length of root, and life percentage of cuttings. Data were analyzed by F test at 1% and 5% and continued with DMRT at 5% if the treatment had a significant effect. It is also continued with regression if there was an interaction between treatments. The longest bud was found on soil + manure + husk (12.28 cm) and followed by soil + manure (10.57 cm) and soil (6.15 cm) in the low level, respectively. The study revealed that the bud length and the number of roots were influenced by auxin concentration. The longest bud was found using 12.5 g/L of auxin (15.14 cm), followed by 50 g/L, 25 g/L, 0 g/L, and 37.5 g/L in the low level, respectively (12.05 cm, 7.72 cm, 7.67 cm, 5.75 cm). The highest number of roots was found on 0 g/L and 12.5 g/L of auxin (7.78), followed by 50 g/L, 25 g/L, and 37.5 g/L in the low level, respectively (4, 3.43, 2.33). The number of roots and percentage of life cuttings showed an interaction between the two factors. The number of roots on the soil shows a parabolic response with auxin. In the beginning, the number of roots decreased and then increased with increasing auxin concentration. The number of roots on soil + manure and soil + manure + husk showed the same pattern with auxin concentration. Almost 100% of life cuttings have been found on all media and auxin concentrations. Soil + manure with 25 g/L auxins and soil + manure + husk with 50 g/L auxins caused 33.33% and 66.67% life cuttings, respectively.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133396635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in color of betta fish (Betta splendens) by feeding of Artemia salina enriched with Tagetes erecta flower flour","authors":"Chika Annisa Kiswara, A. Budiharjo, S. Sari","doi":"10.13057/cellbioldev/v040202","DOIUrl":"https://doi.org/10.13057/cellbioldev/v040202","url":null,"abstract":"Abstract. Kiswara CA, Budiharjo A, Sari SLA. 2020. Changes in color of betta fish (Betta splendens) by feeding of Artemia salina enriched with Tagetes erecta flower flour. Cell Biol Dev 4: 46-50. Betta fish are attractive due to their varied and vibrant colors and aggressive temperament. Carotenoids are the pigments found in betta fish. They are kept in chromatophores. While fish cannot synthesize carotenoids in their bodies, they can absorb them from their diet and use them as pigments to enhance the color intensity of their bodies. The purpose of this study was to determine the degree of color change and survival of betta fish fed with Artemia salina (Linnaeus, 1758), which has Tagetes erecta L. flower flour as their food. This study used a completely randomized design (CRD) and three treatments with varying compositions of rice flour: T. erecta flower flour, namely, 5:1, 2:1, and 1:1, respectively. Artemia salina fed simply with rice flour was a control. Betta fish require 40 days of care. Color intensity was determined once every ten days using a color scale with a score of 1 (white), 2 (weak red), 3 (medium red), and 4 (dark red) (red). The results indicated that A. salina fed in a 1:1 ratio had the best effect on improving the color brightness of betta fish. All parts of the betta fish's body received a score of 4 in this treatment, including the head, fins, and tail.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132641620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Michael Sunday Abu, O. Yakubu, Job Itanyi Onuche, O. Okpe
{"title":"Lipid profiles, hematological parameters and histopathological analysis of CCl4-intoxicated wistar albino rats treated with n-butanol extract of Ficus glumosa leaves","authors":"Michael Sunday Abu, O. Yakubu, Job Itanyi Onuche, O. Okpe","doi":"10.13057/cellbioldev/v060102","DOIUrl":"https://doi.org/10.13057/cellbioldev/v060102","url":null,"abstract":"Abstract. Abu MS, Yakubu OE, Onuche JI, Okpe O. 2022. Lipid profiles, hematological parameters and histopathological analysis of CCl4-intoxicated wistar albino rats treated with n-butanol extract of Ficus glumosa leaves. Cell Biol Dev 6: 6-12. This research critically assessed the effect of the n-butanol fraction of methanol extract of Ficus glumosa Delile leaves on serum lipid profile, hematological parameters, and some organ architecture of experimental albino rats intoxicated with carbon tetrachloride. The crude methanol extract was re-dissolved in 300 mL of distilled water and repeatedly partitioned in a separating funnel with 400 mL of n-hexane with vigorous shaking. This process was repeated using other solvents (ethyl acetate, n-butanol, and distilled water), and the n-butanol was selected based on antioxidant potency. A total of 35 albino rats were used. The rats were divided into 7 groups of 5 animals each. Lipid profile, hematological indices, and histopathological analysis were carried out. With the administration of the extract, triacylglycerol and total cholesterol levels in the Carbon tetrachloride-induced but treated rats were significantly (P<0.05) reduced compared to the normal levels, 1.84±0.27 mmol/L, and 0.54±0.11 mmol/L, respectively. In contrast, high-density lipoprotein was relatively increased compared to the normal level, 1.44±0.43 mg/dL. Similarly, packed cell volume, hemoglobin, and white blood cell levels were significantly (P<0.05) reversed to near normal in the extract-treated rats. On the other hand, the histopathological examinations of a liver section of the normal control group showed normal hepatocellular architecture with distinct hepatic cells with a well-conserved central vein. Carbo tetrachloride-induced control group liver showed intense hepatic necrosis with vascular congestion, kupffer cells hyperplasia, vacuolation, and degeneration of normal hepatic cells (hepatic necrosis). However, the induced but treated groups almost normalized the hepatic cells (moderate hepatic necrosis). The findings showed that the n-butanol fraction of F. glumosa could reverse the deleterious effects of CCl4 on lipid profile and hematological parameters with the restoration of the architectural integrities of the liver and kidney of the treated rats.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129227604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isra Cahayani Bahuwa, D. Lamondo, Abubakar Sidik Katili
{"title":"Short Communication: Papaya (Carica papaya) seed extract test againts Spodoptera litura mortality","authors":"Isra Cahayani Bahuwa, D. Lamondo, Abubakar Sidik Katili","doi":"10.13057/cellbioldev/v060101","DOIUrl":"https://doi.org/10.13057/cellbioldev/v060101","url":null,"abstract":"Abstract. Bahuwa IC, Lamondo D, Katili AS. 2022. Short Communication: Papaya (Carica papaya) seed extract test against Spodoptera litura mortality. Cell Biol Dev 6: 1-5. This study aims to determine the effect of papaya (Carica papaya L.) seed extract on mortality, the most influential concentration of papaya seed extract, Lethal Concentration (LC50) 24 hours, and Lethal Time (LT50) 24 hours armyworm (Spodoptera litura Fabricius, 1775) larvae instar III. The research was carried out at the Biology Department, Universitas Negeri Gorontalo, Indonesia, in June 2021. The method used was an experiment with a Completely Randomized Design consisting of 9 treatments and 3 replications, there are A (aquadest control), B (CMC control), C (1 ppm), D (50 ppm), E (100 ppm), F (200 ppm), G (400 ppm), H (800 ppm), and I (1600 ppm). The data were analyzed by probit analysis LC50, LT50, and One Way Anova. The results showed that the LC50 value was at a dose of 489 ppm. The value of LT50 24 hours at a concentration of 1600 ppm is 10.61 hours, with the fastest time to kill 50% of armyworm larvae. Papaya seed extract affects the mortality of armyworm larvae; the higher the concentration, the less time it takes to kill 50% of the larvae.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131228111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of photoperiod and KNO3 concentration on the induction and development of potato (Solanum tuberosum) microtuber in vitro","authors":"J. P. Choirunnisa, R. Wardana","doi":"10.13057/cellbioldev/v050203","DOIUrl":"https://doi.org/10.13057/cellbioldev/v050203","url":null,"abstract":"Abstract. Choirunnisa JP, Wardana R. 2021. Effect of photoperiod and KNO3 concentration on the induction and development of potato (Solanum tuberosum) microtuber in vitro. Cell Biol Dev 5: 70-75. Potato (Solanum tuberosum L.) is a carbohydrate source plant that was developed as an alternative to food diversification. The availability of quality potato seeds is very limited due to the high attack rate of bacteria and viruses. The demand for potato seeds can be fulfilled by developing potato microtuber through the application of plant tissue culture. The purpose of this research was to determine the response of potato microtuber formation with different photoperiods and KNO3 concentrations. This study design uses a factorial Completely Randomized Design (CRD) with 5 replicates. The first factor is 3 levels of photoperiod (8 hours/day, 12 hours/day, 16 hours/day). The second factor is 3 levels of KNO3 (1900 mg/L, 2850 mg/L, 3800 mg/L). The study was conducted by observing the age of microtubers initiation, number of shoots, number of roots, number of microtubers, the diameter of microtubers, and wet weight of microtubers were analyzed using SPSS. The results showed that the combination treatment of 8 hours/day photoperiod and KNO3 concentration of 3800 mg/L could accelerate the initiation of microtubers at 8 DAP (Days After Planting). The concentration of 3800 mg/L KNO3 can increase the number of roots and microtubers, the diameter of microtubers, and the wet weight of microtubers. The highest diameter of microtubers (17.89 mm) and the highest wet weight of microtubers (278.81 mg) were found in the photoperiod of 16 hours/day. This study concludes that the higher concentration of KNO3 and the longer photoperiod could be used for the induction and development of potato microtubers, while the short photoperiod could be used to accelerate microtubers initiation","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114316293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}