Indonesian Journal of Medical Sciences and Public Health最新文献

Anti-Cancer Assessment of a Ramie (Boehmeria nivea L. Gaud.) Leaf Extract Using Mcf-7 Cell Line and a Yeast-Based Bioassay 一种苎麻(Boehmeria nivea L. Gaud.)的抗癌评价利用Mcf-7细胞系提取叶提取物及酵母生物测定

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-11-23 DOI: 10.11594/ijmp.02.01.01

Asri Peni Wulandari, Annisa Abdiwijaya Qaromah, Karen Kezia Lolowang, D. H. P. Huspa, Ade Zuhrotun

{"title":"Anti-Cancer Assessment of a Ramie (Boehmeria nivea L. Gaud.) Leaf Extract Using Mcf-7 Cell Line and a Yeast-Based Bioassay","authors":"Asri Peni Wulandari, Annisa Abdiwijaya Qaromah, Karen Kezia Lolowang, D. H. P. Huspa, Ade Zuhrotun","doi":"10.11594/ijmp.02.01.01","DOIUrl":"https://doi.org/10.11594/ijmp.02.01.01","url":null,"abstract":"Introduction: One strategy for molecular cancer therapy is to know the key mechanism of cytotoxic compounds that can kill cancer cells. Ramie (Boehmeria nivea L. Gaud.) leaves contain active compounds that have important effects on cancer chemoprevention. Objective: To obtain the active fraction of a Ramie leaf extract in inhibiting the proliferation of MCF-7 breast cancer cell lines and to determine the mechanism of apoptosis induction using MCF-7 and Saccharomyces cerevisiae strains 1140, 1353, and 1138. Method: Fractions were prepared using n-hexane, dichloromethane (CH2Cl2), ethyl acetate, and n-butanol as solvents. All fractions were tested qualitatively through phytochemical. The MTT-based cytotoxicity assay used MCF-7 (in vitro) to obtain the IC50 value, whereas the model system that targets the enzymatic (topoisomerase) used a yeast-based bioassay to obtain the IC12 value. Apoptotic induction of the active fraction in MCF-7 was performed using flow cytometry and qPCR (2-ΔΔCt method). Results: The phytochemical analysis indicated that the extract fraction consisted of alkaloids and steroids. The smallest IC50 value was obtained from the CH2Cl2 fraction as 3.79 g/mL, potentially act as an anticancer. A higher percentage of apoptosis than that of necrotizing cells and live cells was observed through flow cytometry. The CH2Cl2 fraction with an IC12 value < 8000 in strains 1140, 1353, and 1138 consistently showed the mechanism of apoptosis induction as topoisomerase I and II inhibitors. Also, another mechanism could be through the intrinsic pathway, indicated by the highest expression level in p53. Conclusions: The CH2Cl2 fraction of Ramie leaves can inhibits the proliferation of MCF-7 cells in the active and strong categories. The CH2Cl2 fraction induces apoptosis by increasing p53 gene expression and inhibiting topoisomerase I and II. Thus, it showed potential as an anticancer drug candidate.","PeriodicalId":145411,"journal":{"name":"Indonesian Journal of Medical Sciences and Public Health","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115513700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship Between Maternal Education and Knowledge on Coverage of Exclusive Breastfeeding in Ten Stunting Locus Villages in Pandeglang District, Banten 万丹攀德郎十个发育迟缓区村母亲教育与纯母乳喂养覆盖率知识的关系

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-10-30 DOI: 10.11594/ijmp.02.01.05

S. Wuryanti, H. Marsiati

{"title":"Relationship Between Maternal Education and Knowledge on Coverage of Exclusive Breastfeeding in Ten Stunting Locus Villages in Pandeglang District, Banten","authors":"S. Wuryanti, H. Marsiati","doi":"10.11594/ijmp.02.01.05","DOIUrl":"https://doi.org/10.11594/ijmp.02.01.05","url":null,"abstract":"Introduction: Until now, the practice of exclusive breastfeeding is still far from the national target. The success of exclusive breastfeeding is determined by many factors, including the mother’s education, knowledge, and awareness about the importance of breastfeeding for child growth. This study aims to determine the relationship between the mother’s level of education and knowledge on exclusive breastfeeding in ten stunting locus villages located in Pandeglang Regency, Banten, Indonesia. Methods: This study uses a cross-sectional design with data collection methods through interviews and direct observation. The population consists of mothers who have children under 2 years of age. The study was conducted for 6 months from November 2019. The variables studied included the mother’s age, occupation, number of children, education, and knowledge of exclusive breastfeeding. Result: As many as 99.6% of respondents have a good level of knowledge about exclusive breastfeeding. The level of education is low at 44.6%, medium at 50.9%, and high at 4.4%. The results showed there was no significant relationship between the mother’s last education and exclusive breastfeeding (p = 0.860) and there was no significant relationship between the mother's level of knowledge and exclusive breastfeeding (p = 0.558). Conclusion: No significant relationship was observed between the mother’s education and knowledge on exclusive breastfeeding in ten stunting locus villages located in Pandeglang Regency, Banten.","PeriodicalId":145411,"journal":{"name":"Indonesian Journal of Medical Sciences and Public Health","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129287823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of otomycosis in patients with cerumen impaction due to ear-phone usage during COVID-19 COVID-19期间使用耳机所致耳膜嵌塞患者耳真菌病的患病率

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-10-30 DOI: 10.11594/ijmp.02.01.04

S. Suresh, Suraj Suresh, Sudha Sivasamy

{"title":"Prevalence of otomycosis in patients with cerumen impaction due to ear-phone usage during COVID-19","authors":"S. Suresh, Suraj Suresh, Sudha Sivasamy","doi":"10.11594/ijmp.02.01.04","DOIUrl":"https://doi.org/10.11594/ijmp.02.01.04","url":null,"abstract":"Background: Otomycosis affects about 9% of patients with otitis externa. One of the predisposing factor is impacted cerumen. Earphone usage causes cerumen impaction. In the light of the recent COVID-19 pandemic, people are housebound due to prolonged lockdown. Hence people are more technology dependent as working from home and studying online has become the norm. Therefore, usage of earphones has proportionately increased, and the impacted cerumen cases have increased. Methods: This cross-sectional study aimed to analyze data from questionnaire to assess the prevalence of otomycosis among patients with impacted cerumen due to excessive earphone usage, establish associations between otomycosis and symptoms such as pain and hearing loss as well as the correlation between fungal growth and the long hours of earphones usage. The relationship between the age of participants and the usage of earphones during the pandemic was also explored. The data was collected from 100 individuals aged 14 to 51 years who sought treatment for symptomatic impacted wax at an ENT clinic in Malaysia. Results: The mean age of participants was 25.9 years. There was a 31% prevalence of otomycosis among these patients. Otalgia was present in 60% of patients with otomycosis (t value 2.94, coefficient 0.27). Approximately 37% of patients had a large air-bone gap indicating conductive hearing loss. There was an insignificant correlation between otomycosis and the longer hours of earphones usage (t value 1.51, coefficient 0.00015). No correlation was found between age of participants and the total hours of earphone usage (t value 0.63, coefficient 0.0012). Conclusion: This study offers initial evidence that earphone usage could be a predisposing factor in developing otomycosis. However, there was no evidence of longer hours of earphones usage increasing the chances of developing otomycosis. Additionally, symptoms like otalgia and conductive hearing loss could be present in both earwax impaction and otomycosis.","PeriodicalId":145411,"journal":{"name":"Indonesian Journal of Medical Sciences and Public Health","volume":"1992 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128617254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin as an Anti-Proliferative Agent in Breast Cancer through RASSF1A, Bax, and Caspase-3 Protein 姜黄素通过RASSF1A、Bax和Caspase-3蛋白在乳腺癌中的抗增殖作用

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-10-29 DOI: 10.11594/ijmp.02.01.03

N. A. Rahmah, H. Harliansyah, F. Suyatna, M. Kanoko, P. Rustamadji, J. Prihartono, A. Bustami, S. Haryono, B. Hernowo

{"title":"Curcumin as an Anti-Proliferative Agent in Breast Cancer through RASSF1A, Bax, and Caspase-3 Protein","authors":"N. A. Rahmah, H. Harliansyah, F. Suyatna, M. Kanoko, P. Rustamadji, J. Prihartono, A. Bustami, S. Haryono, B. Hernowo","doi":"10.11594/ijmp.02.01.03","DOIUrl":"https://doi.org/10.11594/ijmp.02.01.03","url":null,"abstract":"Introduction: Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specific mechanism of curcumin cytotoxicity remains controversial because of many anti- and pro-apoptotic signaling pathways in various cell types. This study aims to examine the relationship among curcumin on RASSF1A, Bax protein levels, and caspase-3 activity in supporting the apoptotic mechanism in CSA03 breast cancer cells. Method: Curcumin administration to cancer cells is based on differences in dosage with 24-hour incubation. Cytotoxicity after curcumin administration was determined using MTS. RASSF1A and Bax protein levels were tested through ELISA. Caspase-3 activity was used to determine apoptosis and was tested using flow cytometry. Results: The results indicated that curcumin had a cytotoxicity effect of 40.85 µg/mL. At concentrations of 40 µg/mL and 50 µg/mL, curcumin increases levels of protein RASSF1A (∆ = 26.53% and 47.35%, respectively), Bax (∆ = 48.79% and 386.15%), and caspase-3 (∆ = 1,678.51% and 1,871.889%) significantly. Conclusions: Curcumin exhibits anti-proliferative activity and apoptotic (Caspase-3) effects through activation of RASSF1A and Bax.","PeriodicalId":145411,"journal":{"name":"Indonesian Journal of Medical Sciences and Public Health","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125980239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attitude and Knowledge Relationship with Sexual Behavior at Risk of Sexually Transmitted Infection (STI) in Male Adolescents in Indonesia (IDHS Data Analysis 2017) 印度尼西亚男性青少年性传播感染(STI)风险与性行为的态度和知识关系(IDHS数据分析2017)

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-10-29 DOI: 10.11594/ijmp.02.01.02

N. Asiah, Agnes Yohana Sondi, N. Parlina, D. Jovanka

{"title":"Attitude and Knowledge Relationship with Sexual Behavior at Risk of Sexually Transmitted Infection (STI) in Male Adolescents in Indonesia (IDHS Data Analysis 2017)","authors":"N. Asiah, Agnes Yohana Sondi, N. Parlina, D. Jovanka","doi":"10.11594/ijmp.02.01.02","DOIUrl":"https://doi.org/10.11594/ijmp.02.01.02","url":null,"abstract":"Introduction: Indonesia Population Demographic Survey in 2017 recorded that 8% of teenage boys and 2% of teenage girls admitted to having sexual intercourse. This certainly worries all of us who hope today's youth will be a demographic bonus. Sexual behavior is at risk of impacting the issue of Unwanted Pregnancy (KTD), abortion, Sexually Transmitted Infections (STIs), and the ongoing incidence of HIV and AIDS. Objective: to confirm the relationship of attitudes and knowledge with risky sexual behavior in adolescent boys in Indonesia. Methods: This type of research is quantitative analytics using a cross-sectional design and sourced from secondary data of the Indonesian Health Demographic Survey 2017. The population in this study was 13,079 adolescent men aged 15-24, sampling 10,710 with saturated sampling techniques. Bivariate data analysis using the chi-square test (0.05). Results: were obtained by adolescents with a positive attitude of 52.1%, knowledgeable 77.8%, and 13.1% risky sexual behavior. Test results showed there was an attitude relationship with risky sexual behavior with p-value = 0.000 and there was a knowledge relationship with risky sexual behavior with p-value = 0.000. Conclusions: This study are expected to be used as input for the creation of risky sexual behavior prevention programs in adolescents and form the basis for the creation of an educational model related to adolescent reproductive health for parents and educational institutions.","PeriodicalId":145411,"journal":{"name":"Indonesian Journal of Medical Sciences and Public Health","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125360887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Adobe Photoshop Express Application Enhances the Diagnosis of X-Ray Thorax of Covid-19 Patient adobephotoshopexpress应用增强对Covid-19患者x线胸廓的诊断

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-10-19 DOI: 10.11594/ijmp.01.02.05

Ahmad Mochtar Jamil, M. Putra, Muhammad Anas

引用次数: 0

Head Size and Intelligence Quotient (IQ) in Elementary School Students 小学生头部大小与智商的关系

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-10-19 DOI: 10.11594/ijmp.01.02.01

Oski Illiandri, D. Setyohadi, Aji Ilman Sjidan, Aldiya Jamila, Adwa Humaira, M. Ibrahim, Ahmad Rofii

{"title":"Head Size and Intelligence Quotient (IQ) in Elementary School Students","authors":"Oski Illiandri, D. Setyohadi, Aji Ilman Sjidan, Aldiya Jamila, Adwa Humaira, M. Ibrahim, Ahmad Rofii","doi":"10.11594/ijmp.01.02.01","DOIUrl":"https://doi.org/10.11594/ijmp.01.02.01","url":null,"abstract":"Superficially, Intelligence is an ability to planning, thinking, talking, rationalization, comparing, and understand with other. One of many methods to rate for intelligence, is Draw-A-Man test, it’s introduced by Goodenough in 1926 to researching children’s creativity, mental age, and visual and motoric intelligence with some code based on picture that the children drawed. The aim of this study was to determine the correlation between head circumference and head length with Intelligence Quotient of the 3rd grade Karang Mekar 1 Banjarmasin elementary school’s student. The methodsof this study used observational analytics with a cross-sectional approach. The sampling technique was total sampling with a sample of 65 students aged 7 to 10 years and fulfilling the inclusion and exclusion criteria. The results of data analysis using the Spearman correlation test showed that the correlation point on the head circumference and head length with IQ were p=0.888 and p=0.985. Based on the results of this study, it can be concluded that there is no significant correlation between the the head circumference and head length with Intelligence Quotien in elementary student.","PeriodicalId":145411,"journal":{"name":"Indonesian Journal of Medical Sciences and Public Health","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128490508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Case of Ingested Laryngoscope Bulb During Emergency Endotracheal Intubation 急诊气管插管时误食喉镜球1例

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-10-19 DOI: 10.11594/ijmp.01.02.04

Saurabh Sharma, Sandhya Gupta, Tushar Nagyan, Md. Abu Nasar, Sowbir Some, P. Agarwal

引用次数: 0

Coronavirus Pandemic: Situation in Nigeria 冠状病毒大流行:尼日利亚的情况

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-02-05 DOI: 10.11594/ijmp.01.02.03

F. Abulude, A. Akinnusotu, S. Oluwagbayide, Usha Damodharan, I. Abulude

{"title":"Coronavirus Pandemic: Situation in Nigeria","authors":"F. Abulude, A. Akinnusotu, S. Oluwagbayide, Usha Damodharan, I. Abulude","doi":"10.11594/ijmp.01.02.03","DOIUrl":"https://doi.org/10.11594/ijmp.01.02.03","url":null,"abstract":"COVID - 19 is an issue ravaging the whole world. Numerous deaths have been recorded particularly elderly individuals and the most vulnerable. In Nigeria, the case isn't extraordinary. The aim of the study was to quantify the number of cases reported in Nigeria. To this effect, the global literature cited in the Nigeria Centre for Disease Control (NCDC) website, the WHO COVID-19 database, other expert-referred scientific articles, and bibliographic databases were used. The results show that as at the sixteenth of May 2020, 288 new affirmed cases and 3 deaths were recorded in Nigeria. Till date, 5445 cases have been affirmed, 778 cases have been released and 171 deaths have been recorded in 34 states and the Federal Capital Territory, While Lagos State is for the most part influenced with 36 deaths in comparison with the cases revealed by the World Health Organization (4,425,485 cases affirmed, 89,269 new cases and 302,059 deaths). The death rate in Nigeria is about 0.05% of the global deaths. The explanation could be because of high temperature (> 30 oC), the intake of garlic, ginger, honey, and heated water by the individuals, and to stop the spread, the Nigerian government has implemented the utilization of nose cover, social distancing, and semi-lockdown of the towns and urban communities. Like HIV and Laser fever this pandemic will be an issue of the past when the adequate vaccine is made available.","PeriodicalId":145411,"journal":{"name":"Indonesian Journal of Medical Sciences and Public Health","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116789224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased Age and Long Working Period Associated with Increased Severi-ty of Pterygium Among Fishermen in Kedung Cowek Sub-District, Surabaya, East Java-Indonesia 印度尼西亚东爪哇泗水Kedung Cowek街道渔民中年龄增加和工作时间长与翼状胬肉严重程度增加有关

Indonesian Journal of Medical Sciences and Public Health Pub Date : 2021-02-05 DOI: 10.11594/ijmp.01.02.02

I. A. Pasaribu, P. Utami

{"title":"Increased Age and Long Working Period Associated with Increased Severi-ty of Pterygium Among Fishermen in Kedung Cowek Sub-District, Surabaya, East Java-Indonesia","authors":"I. A. Pasaribu, P. Utami","doi":"10.11594/ijmp.01.02.02","DOIUrl":"https://doi.org/10.11594/ijmp.01.02.02","url":null,"abstract":"Pterygium is a degenerative and invasive disorder of the subconjunctival tissue that can reach the cornea area which is become a worldwide health problem. UV exposure is the biggest risk factor for pterygium. A profession as a fisherman is one of the occupations that are often related to the appearance of pterygium, because of long-term exposure to UV rays. This study aimed to determine the correlation between age and duration as a fisherman with the degree of pterygium in Kedung Cowek village, Bulak Surabaya district, East Java - Indonesia. The research design was an analytic observational using consecutive sampling during March - July 2011; with the total number of respondents is 42 fishermen who met the inclusion criteria. The results of the statistical analysis test showed that there was a strong and significant relationship between the age of the fishermen (p = 0.007; coefficient correlation = 502) and the duration of working as a fisherman (p = 0.029; coefficient correlation = 409) with the severity of the pterygium. Based on these results it can be concluded that the age and duration of work as fishermen are related to the severity of the pterygium in fishermen in the Kedung Cowek sub-district, Surabaya, East Java-Indonesia.","PeriodicalId":145411,"journal":{"name":"Indonesian Journal of Medical Sciences and Public Health","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115070338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0