Japanese journal of medical science & biology最新文献_第8页

Characterization of VP4 and VP7 of a murine rotavirus (YR-1) isolated in Japan. 日本分离鼠轮状病毒(YR-1) VP4和VP7的鉴定

Japanese journal of medical science & biology Pub Date : 1995-10-01 DOI: 10.7883/yoken1952.48.237

H Ushijima, S Morikawa, A Mukoyama, O Nishio

引用次数: 9

Effect of lupeol, a pentacyclic triterpene, on urinary enzymes in hyperoxaluric rats. 五环三萜芦皮醇对高血氧大鼠尿酶的影响。

Japanese journal of medical science & biology Pub Date : 1995-10-01 DOI: 10.7883/yoken1952.48.211

M M Malini, R Baskar, P Varalakshmi

{"title":"Effect of lupeol, a pentacyclic triterpene, on urinary enzymes in hyperoxaluric rats.","authors":"M M Malini, R Baskar, P Varalakshmi","doi":"10.7883/yoken1952.48.211","DOIUrl":"https://doi.org/10.7883/yoken1952.48.211","url":null,"abstract":"Investigations were undertaken to study the role of lupeol, a pentacyclic triterpene from Crataeva nurvala stem bark, in calcium oxalate experimental rat urolithiasis. A 2% solution of ammonium oxalate was administered by gastric intubation for inducing hyperoxaluric condition in adult male rats of Wistar strain. The duration of treatment was for 15 days. This resulted in increased urinary excretion of oxalate associated with reduction in citrate and glycosaminoglycans. The urinary marker enzymes which indicate renal tissue damage namely--lactate dehydrogenase, inorganic pyrophosphatase, alkaline phosphatase, gamma glutamyl transferase, beta-glucuronidase and N-acetyl beta-D glucosaminidase were found to be elevated. Lupeol administration (25 mg/kg body weight/day) reduced significantly the renal excretion of oxalate. It also reduced the extent of renal tubular damage as evidenced from the decreased levels of the above enzymes in urine. Such a reduction is likely to be beneficial in minimizing the deposition of stone-forming constituents in the kidney which provides antilithic effect.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"48 5-6","pages":"211-20"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.48.211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19693134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Titration of varicella-zoster virus DNA in throat swabs from varicella patients by combined use of PCR and microplate hybridization. PCR和微孔板杂交联合测定水痘患者咽拭子中水痘-带状疱疹病毒DNA。

Japanese journal of medical science & biology Pub Date : 1995-10-01 DOI: 10.7883/yoken1952.48.249

R Hondo, S Ito, S Inouye

引用次数: 11

Non-radioactive mismatch analysis to detect small mutations in human hypoxanthine-guanine phosphoribosyl transferase cDNA. 非放射性错配分析检测人次黄嘌呤-鸟嘌呤磷酸核糖基转移酶cDNA的小突变。

Japanese journal of medical science & biology Pub Date : 1995-08-01 DOI: 10.7883/yoken1952.48.163

K Tsuboi, T Nose, R T Okinaka, D J Chen

引用次数: 3

Study of hemagglutinating property of enteroinvasive Escherichia coli from various geographical locations. 不同地区侵入性大肠杆菌血凝特性的研究。

Japanese journal of medical science & biology Pub Date : 1995-08-01 DOI: 10.7883/yoken1952.48.193

S H Bhuiyan, H Rahman, K Haider

引用次数: 2

Semiquantitative analysis of periodontopathogens by gene amplification. 牙周病原基因扩增半定量分析。

Japanese journal of medical science & biology Pub Date : 1995-08-01 DOI: 10.7883/yoken1952.48.177

Y Sumi, Y Yamashita, Y Nakano, T Koga

{"title":"Semiquantitative analysis of periodontopathogens by gene amplification.","authors":"Y Sumi, Y Yamashita, Y Nakano, T Koga","doi":"10.7883/yoken1952.48.177","DOIUrl":"https://doi.org/10.7883/yoken1952.48.177","url":null,"abstract":"Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis, which are known as major causative organisms of periodontitis, were semiquantitatively identified by two-step polymerase chain reaction (PCR). The sets of specific primers for A. actinomycetemcomitans and P. gingivalis were prepared on the basis of the nucleotide sequences of the lktA and the fimA genes, respectively. The set of universal primers for eubacteria was designed from the nucleotide sequence of a highly conserved region in the eubacterial 16S rRNA sequence. The number of bacteria detectable by one-step PCR assay was no fewer than 10(3) cells. Less than 10(3) bacterial cells were detectable by two-step PCR assay. Subgingival plaque samples from 37 sites of 16 patients were obtained with paperpoints and analyzed by two-step PCR assay. More than 2 x 10(6) bacterial cells were found in the subgingival plaque samples from all diseased sites. In contrast, the number of total bacteria in those from more than half of healthy sites estimated by PCR assay was less than 2 x 10(6) cells, suggesting that subgingival plaque in diseased sites consists of a relatively larger number of bacteria compared with the population in healthy sites. While A. actinomycetemcomitans was detected in both healthy and diseased sites, P. gingivalis was observed only in diseased sites. Both periodontopathic bacteria occupied a minor part (less than 0.1%) of the total subgingival plaque bacteria.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"48 4","pages":"177-91"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19549561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Adenovirus surveillance, 1982-1993, Japan. A report of the National Epidemiological Surveillance of Infectious Agents in Japan. 腺病毒监测，1982-1993年，日本。日本全国传染病流行病学监测报告。

Japanese journal of medical science & biology Pub Date : 1995-08-01

S Yamadera, K Yamashita, M Akatsuka, N Kato, M Hashido, S Inouye, S Yamazaki

{"title":"Adenovirus surveillance, 1982-1993, Japan. A report of the National Epidemiological Surveillance of Infectious Agents in Japan.","authors":"S Yamadera, K Yamashita, M Akatsuka, N Kato, M Hashido, S Inouye, S Yamazaki","doi":"","DOIUrl":"","url":null,"abstract":"The Infectious Agents Surveillance Center, the National Institute of Health, Japan, received 17,265 reports from 1982 to 1993 on cases from whom adenovirus was isolated or detected; 85% from 57 public health institutes and the other 15% from two national hospitals and two commercial diagnostic laboratories. The followings were found. Three major diseases caused by adenovirus were upper respiratory tract infection, gastroenteritis, and conjunctivitis. Patients of upper respiratory tract infection numbered 6,837 (40% of all patients due to adenovirus), the identified serotypes being in order of frequency types 3, 2, 1, and 5. Those of gastroenteritis numbered 1,636 (9.5%). From 40% of the gastroenteritis patients, adenovirus was detected by electron microscopy or immunochemical methods without cultivation. From the remaining 60%, virus was isolated in tissue culture; the serotypes of the isolates resembled those causing upper respiratory tract infection. Patients of conjunctivitis numbered 3,437 (20%), the frequency being in order of types 3, 4, 8, 37, and 19. Conjunctivitis due to types 3 and 4 prevailed every summer; type 3 was isolated often from children with pharyngo-conjunctival fever and the other four types were mostly from adults with epidemic keratoconjunctivitis. Type 3 had a unique feature not seen in other types: it was most frequently isolated, causing upper respiratory tract infection, gastroenteritis, conjunctivitis, and pharyngo-conjunctival fever. Reports on isolation of type 7, which has been reported to cause severe pneumonia in many other countries, were as few as 28 (0.2%).","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"48 4","pages":"199-210"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19549473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mark-release-recapture experiments to estimate the efficiency of the light trap in collecting Japanese encephalitis vector mosquitoes. 标记-释放-再捕获实验评价诱蚊灯捕获日本脑炎病媒蚊虫的效果。

Japanese journal of medical science & biology Pub Date : 1995-06-01 DOI: 10.7883/yoken1952.48.123

M Takagi, Y Tsuda, Y Wada, A Sugiyama, H Shimomura, W Suwonkerd, A Suzuki

引用次数: 0

Effect of administering cyclophosphamide and vitamin E on the levels of tumor-marker enzymes in rats with experimentally induced fibrosarcoma. 给予环磷酰胺和维生素E对实验性纤维肉瘤大鼠肿瘤标记酶水平的影响。

Japanese journal of medical science & biology Pub Date : 1995-06-01 DOI: 10.7883/yoken1952.48.145

R Vinitha, M Thangaraju, P Sachdanandam

{"title":"Effect of administering cyclophosphamide and vitamin E on the levels of tumor-marker enzymes in rats with experimentally induced fibrosarcoma.","authors":"R Vinitha, M Thangaraju, P Sachdanandam","doi":"10.7883/yoken1952.48.145","DOIUrl":"https://doi.org/10.7883/yoken1952.48.145","url":null,"abstract":"Cyclophosphamide, and antineoplastic drug, and vitamin E, the common antioxidant present in the diet, were administered in separate dosages and in combination to animals (rats) with fibrosarcoma, metastatic tumor of the connective tissues, induced. The anticancer drug (20 mg/kg body weight) and the vitamin-E (400 mg/kg body weight) was administered for a period of 28 days from the day of tumor transplantation. The individual and the combined effects of these two substances were investigated by checking the growth of the tumor. Tumor markers like lactate dehydrogenase (LDH), serum glutamate pyruvate transminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), acid phosphatase, and alkaline phosphatase were analyzed for the changes in their concentration in serum, liver, and kidney to assess the success of the therapy. The increased level of the enzymes in the fibrosarcoma-suffering rats (GPII) was reduced by cyclophosphamide treatment (GP III) and vitamin E administration (GP IV). Among the treated groups, the combination therapy (GP V) showed greater efficacy in the treatment of fibrosarcoma than did individual administration, as there was more reduction in the levels of enzymes in Group V than those in to Groups III and IV. The enzyme levels were brought to near the normal level.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"48 3","pages":"145-56"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.48.145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19513602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Human alveolar echinococcosis seroprevalence assessed by western blotting in Hokkaido. 用免疫印迹法测定北海道地区人肺泡包虫病血清阳性率。

Japanese journal of medical science & biology Pub Date : 1995-06-01 DOI: 10.7883/yoken1952.48.157

H Nagano, C Sato, K Furuya

引用次数: 5