Investigacion clinica最新文献_第9页

Drug-disease interactions of differentially expressed genes in COVID-19 liver samples: an in-silico analysis COVID-19肝脏样本中差异表达基因的药物-疾病相互作用:一项计算机分析

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-11-29 DOI: 10.22209/ic.v62n4a03

Susan Omar Rasool, Ata Mirzaei Nahr, Sania Eskandari, Milad Hosseinzadeh, Soheila Asoudeh Moghanloo, F. Ebrahimzadeh

{"title":"Drug-disease interactions of differentially expressed genes in COVID-19 liver samples: an in-silico analysis","authors":"Susan Omar Rasool, Ata Mirzaei Nahr, Sania Eskandari, Milad Hosseinzadeh, Soheila Asoudeh Moghanloo, F. Ebrahimzadeh","doi":"10.22209/ic.v62n4a03","DOIUrl":"https://doi.org/10.22209/ic.v62n4a03","url":null,"abstract":"While COVID-19 liver injuries have been reported in various studies, concerns are raised about disease-drug reactions in COVID-19 patients. In this study, we examined the hypothesis of gene-disease interactions in an in-silico model of gene expression to seek changes in cytochrome P450 genes. The Gene Expression Omnibus dataset of the liver autopsy in deceased COVID-19 patients (GSE150316) was used in this study. Non-alcoholic fatty liver biopsies were used as the control (GSE167523). Besides, gene expression analysis was performed using the DESeq/EdgeR method. The GO databases were used, and the paths were set at p<0.05. The drug-gene interaction database (DGIdb) was searched for interactions. According to the results, 5,147 genes were downregulated, and 5,122 genes were upregulated in SARS-CoV-2 compared to healthy livers. Compared to the cytochromes, 34 cytochromes were downregulated, while 4 cytochromes were upregulated among the detected differentially expressed genes (DEG). The drug-gene interaction database (DGIdb) provided a list of medications with potential interactions with COVID-19 as well as metacetamol, phenethyl isocyanate, amodiaquine, spironolactone, amiloride, acenocoumarol, clopidogrel, phenprocoumon, trimipramine, phenazepam, etc. Besides, dietary compounds of isoflavones, valerian, and coumarin, as well as caffeine metabolism were shown to have possible interactions with COVID-19 disease. Our study showed that expression levels of cytochrome P450 genes could get altered following COVID-19. In addition, a drug-disease interaction list is recommended to be used for evaluations in clinical considerations in further studies.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44085537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Publicaciones científicas sobre COVID-19 ¿La evidencia presentada es de alta calidad? 关于新型冠状病毒的科学出版物所提供的证据是高质量的吗？

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-09-02 DOI: 10.22209/ic.v62n3a00

G. Vizcaíno

引用次数: 0

Chlamydia trachomatis. Co-factor or factor in cancer of the cervix? 沙眼衣原体。子宫颈癌的辅助因素还是因素?

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-09-02 DOI: 10.22209/IC.V62N3A06

José Núñez Troconis

引用次数: 0

Estudio epidemiológico de histoplasmosis, paracoccidioidomicosis y criptococosis en una zona urbana de Ciudad Guayana, estado Bolívar, Venezuela 委内瑞拉玻利瓦尔州瓜亚那市城市地区组织胞浆菌病、副球孢子菌病和隐球菌病的流行病学研究

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-09-02 DOI: 10.22209/ic.v62n3a01

J. R. Cermeño, Ernesto Alayo, J. J. Cermeño, Alfredo Calzadilla, A. Rodríguez, Salvador Penna

{"title":"Estudio epidemiológico de histoplasmosis, paracoccidioidomicosis y criptococosis en una zona urbana de Ciudad Guayana, estado Bolívar, Venezuela","authors":"J. R. Cermeño, Ernesto Alayo, J. J. Cermeño, Alfredo Calzadilla, A. Rodríguez, Salvador Penna","doi":"10.22209/ic.v62n3a01","DOIUrl":"https://doi.org/10.22209/ic.v62n3a01","url":null,"abstract":"Se realizó un estudio prospectivo con el objetivo de determinar la prevalencia de infecciones por Histoplasma capsulatum, Complejo Paracoccidioides spp. y Complejo Cryptoccocus en individuos que viven o trabajan en la Plaza de las Ciencias del Sur, Puerto Ordaz, en el estado Bolívar, Venezuela, donde se refugian aves migratorias. Se aplicó una encuesta clínico-epidemiológica y se administraron pruebas intradérmicas de histoplasmina y paracoccidiodina, a todos los sujetos participantes en el estudio. A aquellos individuos sintomáticos respiratorios crónicos, se les realizó una evaluación clínica, radiológica, cultivo micológico y estudio serológico (anticuerpos específicos para Histoplasma capsulatum, Complejo Paracoccidioides spp. y del antígeno capsular del Complejo Cryptococcus spp.). Se aplicaron 632 pruebas intradérmicas a 316 individuos. De estos, sólo 296 (93,7%) acudieron a la lectura de las pruebas. Los sujetos tenían una media de edad de 40,3 años (rango: 7-76 años). Las manifestaciones clínicas más frecuentes fueron: tos (n=49; 16,5%), expectoración (n=17; 5,7%) y fiebre (n=8; 2,7%). La prueba para la histoplasmina fue positiva en el 47,3% (n=140) y a la paracoccidioidina, en el 32,4% (n=96). Los factores de riesgos fueron: contacto con palomas 30,4% (n=90) y visitas a fincas con gallineros 29,1% (n=86). Se identificaron dos casos de paracoccidioidomicosis pulmonar (0,7%) y un caso (0,3%) de criptococosis pulmonar. La elevada prevalencia de infección por Histoplasma capsulatum (47,3%) y Paracoccidioides spp. (32,4%) en ambientes urbanos del estado Bolívar, demuestra la necesidad de promover la búsqueda activa de estos hongos patógenos en quienes tienen síntomas respiratorios crónicos, que no mejoran con terapia antibacteriana y que habitan en zonas con características ambientales y geográficas, propicias para el desarrollo de estos hongos.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43151659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemorrhoidal artery ligation without using a doppler guide: Is it a feasible and safe technique? 不使用多普勒导管结扎痔疮动脉：这是一种可行且安全的技术吗？

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-09-02 DOI: 10.22209/ic.v62n3a04

M. Degerli, Doğan Yıldırım, M. Cakir, O. Akturk, Orçun Alpay, Alp Omer Canturk, O. F. Kandaz, M. Akıncı

{"title":"Hemorrhoidal artery ligation without using a doppler guide: Is it a feasible and safe technique?","authors":"M. Degerli, Doğan Yıldırım, M. Cakir, O. Akturk, Orçun Alpay, Alp Omer Canturk, O. F. Kandaz, M. Akıncı","doi":"10.22209/ic.v62n3a04","DOIUrl":"https://doi.org/10.22209/ic.v62n3a04","url":null,"abstract":"The objective of this work was to analyze the results of the hemorrhoidal artery ligation technique without using a doppler guide, in patients with grade 3 hemorrhoidal disease; by evaluating cost-effectiveness, operation time, recurrence rate, postoperative pain, and secondary outcomes. A hemorrhoidal artery ligation procedure, without using a doppler guide, was performed on 43 patients (15 females, 28 males) with grade 3 symptomatic hemorrhoidal disease, from June 2015 to June 2019, in the Haseki Training and Research Hospital, Istanbul, Turkey. Patients were followed up clinically for one month. They completed a questionnaire within one year after their procedure. The median age was 46 years (range: 24 to 82 years). The main complaints were bleeding in 27 patients, pain in 22 patients, and skin tag in 14 patients. The mean preoperative VAS score was 3.4. The mean operating time was 18 mins (range: 13 to 25 min). All patients remained hospitalized for 24 h. Reoperation was necessary for only one patient because of bleeding. One year after the surgery, the pain was resolved in 21 (95.4%) out of 22 patients with preoperative pain, and bleeding resolved in 25 (92.5%) out of 27 patients with preoperative bleeding. The hemorrhoidal artery ligation is a simple method with a shorter learning curve producing similar postoperative results to other surgical procedures. The surgical technique is cost-effective as it does not require any particular device (anoscope with Doppler ultrasound) to apply.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48525810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The landscape of diabetic foot in Peru 秘鲁糖尿病足的景观

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-09-02 DOI: 10.22209/ic.v62n3a07

Ericka Saravia-Hernández, J. Salvador-Carrillo, Alejandra Zevallos, Jorge Calderón-Ticona

引用次数: 0

Exactitud de pruebas clínicas para verificar la adecuada colocación del catéter venoso central en Urgencias 临床试验验证急诊中心静脉导管正确放置的准确性

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-09-02 DOI: 10.22209/ic.v62n3a03

Jorge Ayón-Aguilar, Norberto Martínez-Luna, Fernando Fernández-Lucas, S. Méndez-Martínez, Patricia Seefoó-Jarquín

{"title":"Exactitud de pruebas clínicas para verificar la adecuada colocación del catéter venoso central en Urgencias","authors":"Jorge Ayón-Aguilar, Norberto Martínez-Luna, Fernando Fernández-Lucas, S. Méndez-Martínez, Patricia Seefoó-Jarquín","doi":"10.22209/ic.v62n3a03","DOIUrl":"https://doi.org/10.22209/ic.v62n3a03","url":null,"abstract":"La utilidad del catéter venoso central (CVC) depende de su adecuada colocación y la radiografía de tórax es el estándar de oro para corroborarla. Para este fin se utilizan pruebas clínicas. El objetivo del presente estudio fue determinar la sensibilidad, especificidad y valores predictivos de cinco pruebas clínicas, para verificar la adecuada colocación del CVC, por lo que se realizó un estudio en 128 pacientes ingresados al servicio de Urgencias, con necesidad de colocación de CVC; se realizaron cinco pruebas clínicas: determinación de arritmias (Ar), retorno venoso (RV), oscilación de presión venosa central (OPVC), medición externa (ME) y gasometría venosa central (GVC); la posición del catéter se corroboró con radiografía de tórax. El 97% presentó adecuada colocación del CVC en la radiografía de tórax; los resultados de sensibilidad (Se), especificidad (Sp), valores predictivos positivo (VPP) y negativo (VPN) de las pruebas fueron: Ar: 59,6%, 50%, 97,3% y 3,8%; RV: 100%, 0%, 96,8% y 0%; OPVC: 99,1%, 25%, 97,6% y 50%; ME: 100%, 0%, 96,8% y 0%; GVC: 96,7%, 0%, 96,7% y 0%, respectivamente. Concluimos que si las pruebas clínicas OPVC, Ar y RV resultan positivas, por sus altos valores de sensibilidad y valor predictivo positivo, podrían sustituir la necesidad de realizar una radiografía de tórax inmediata y no retrasar el uso del CVC, siempre y cuando no se sospechen complicaciones secundarias tempranas a la colocación del mismo.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43342893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detección de anticuerpos contra Histoplasma capsulatum, complejo Paracoccidioides y complejo Sporothrix schenckii en Canis familiaris, mediante la prueba de inmunodifusión en gel de agarosa. municipio Caroní, estado Bolívar, Venezuela 用琼脂糖凝胶免疫扩散试验检测家犬胶囊组织胞浆菌、副球虫复合物和申克孢子丝菌复合物的抗体。委内瑞拉玻利瓦尔州卡罗尼市

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-09-02 DOI: 10.22209/ic.v62n3a02

J. R. Cermeño, J. Ortiz, A. K. Quintero

{"title":"Detección de anticuerpos contra Histoplasma capsulatum, complejo Paracoccidioides y complejo Sporothrix schenckii en Canis familiaris, mediante la prueba de inmunodifusión en gel de agarosa. municipio Caroní, estado Bolívar, Venezuela","authors":"J. R. Cermeño, J. Ortiz, A. K. Quintero","doi":"10.22209/ic.v62n3a02","DOIUrl":"https://doi.org/10.22209/ic.v62n3a02","url":null,"abstract":"Los hongos dimórficos, Histoplasma capsulatum, complejo Paracoccidioides spp. y complejo Sporothrix schenckii son los agentes causales de la histoplasmosis, paracoccidioidomicosis y esporotricosis, respectivamente. El objetivo de este estudio fue evaluar la presencia de anticuerpos contra H. capsulatum, complejo Paracoccidioides spp. y complejo Sporothrix schenckii en perros domésticos (Canis familiaris) del municipio Caroní, estado Bolívar, Venezuela. Se realizó un estudio prospectivo, descriptivo y transversal. Se recolectaron al azar 200 muestras de suero de igual número de perros pertenecientes a 10 localidades del municipio Caroní. Mediante la técnica de inmunodifusión en gel de agarosa, se buscaron anticuerpos específicos contra antígenos de H. capsulatum, complejo Paracoccidioides spp. y complejo Sporothrix schenckii. El 9,0% (n=18) de los caninos estudiados mostraron anticuerpos contra H. capsulatum, 7,0% (n=14) contra Paracoccidioides spp., 1,0% (n=2) para ambos hongos y 1,5% (n=3) para complejo Sporothrix schenckii; siendo ubicados, la mayoría de estos, en las localidades de San Félix y Unare. La detección de anticuerpos específicos contra Histoplasma capsulatum, complejo Paracoccidioides spp. y complejo S. schenckii en los perros, sugiere que estos agentes están presentes en el municipio Caroní. Probablemente, los humanos que habitan esta zona tengan una exposición similar a estos hongos y por tanto, el diagnóstico de las condiciones causadas por estos agentes debe ser considerado en esta área geográfica.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49449790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatocyte Nuclear Factor -1α stimulates cervical cancer cells to migrate and invade through regulating pyruvate kinase L/R 肝细胞核因子-1α通过调节丙酮酸激酶L/R刺激宫颈癌细胞迁移和侵袭

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-09-02 DOI: 10.22209/ic.v62n3a05

Xiao-ling Tao, Wei-chang Yu, Dejun Chen, Liming Wang, Lu Liu, Q. Xing

{"title":"Hepatocyte Nuclear Factor -1α stimulates cervical cancer cells to migrate and invade through regulating pyruvate kinase L/R","authors":"Xiao-ling Tao, Wei-chang Yu, Dejun Chen, Liming Wang, Lu Liu, Q. Xing","doi":"10.22209/ic.v62n3a05","DOIUrl":"https://doi.org/10.22209/ic.v62n3a05","url":null,"abstract":"This study was aimed to analyze the role of hepatocyte nuclear factor -1α (HNF-1α) in regulating migrative and invasive potentials in cervical cancer via the involvement of pyruvate kinase L/R (PKLR). The expression of HNF-1α and PKLR in cervical cancer tissues classified by tumor size and FIGO (Federation International of Gynecology and Obstetrics) stage were detected by qRT-PCR. The expression correlation between HNF-1α and PKLR in cervical cancer tissues was analyzed by Pearson correlation test. After intervening HNF-1α and PKLR levels in SiHa and Hela cells, their migratory and invasive abilities were examined by the Transwell assay. HNF-1α was upregulated in cervical cancer tissues, particularly those with large tumor size or advanced FIGO stage. PKLR was highly expressed in cervical cancer tissues as well, presenting a positive correlation with the HNF-1α level. Knockdown of HNF-1α attenuated migratory and invasive abilities in SiHa cells, whereas overexpression of HNF-1α enhanced migratory and invasive abilities in SiHa cells. PKLR was able to abolish the regulatory effects of HNF-1α on cervical cancer metastasis. HNF-1α and PKLR synergistically promote cervical cancer to migrate and invade.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44677777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2: Potential feco-oral transmission and implications on the spread and severity of COVID-19 in Venezuela. Mini-review SARS-CoV-2:潜在的经口传播及其对委内瑞拉COVID-19传播和严重程度的影响本文着重

IF 0.2 4区医学

Investigacion clinica Pub Date : 2021-07-30 DOI: 10.22209/IC.v62s2a05

L. Chacin-Bonilla, N. Chacón

{"title":"SARS-CoV-2: Potential feco-oral transmission and implications on the spread and severity of COVID-19 in Venezuela. Mini-review","authors":"L. Chacin-Bonilla, N. Chacón","doi":"10.22209/IC.v62s2a05","DOIUrl":"https://doi.org/10.22209/IC.v62s2a05","url":null,"abstract":"The recognized human-to-human transmission of SARS-CoV-2 is through respiratory droplets and contact with contaminated surfaces. However, the high transmissibility of the virus and the pattern of symptoms of COVID-19 suggest the likelihood of other forms of spread. Increasing evidence suggests that SARS‐CoV‐2 could be transmitted by the feco‐oral route. SARS-CoV-2 is known to infect gastrointestinal epithelial cells and a significant number of infected people have gastrointestinal symptoms. Viable viruses, viral RNA, and prolonged shedding of viral RNA have been detected in the feces of COVID-19 patients. The virus has been found in sewage and surface waters of several countries. The possible feco-oral transmission of SARS-CoV-2 could be significant in low-income countries. High poverty levels and the collapse of health and other public services might increase the risk of Venezuelans to suffer a more devastating impact from COVID-19 than other populations. In conclusion, the feco-oral transmission of SARS-CoV-2 has not been demonstrated. However, it is conceivable and the impact of COVID-19 could be high in low-income countries, especially in Venezuela due to its humanitarian crisis. The lack of information on the viability and infectivity of the virus in wastewaters and surface waters and the risk of transmission of the infection are important gaps in knowledge that deserve further investigation.","PeriodicalId":14514,"journal":{"name":"Investigacion clinica","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2021-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42806727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1