IntraVital最新文献_第3页

Comparison of intravital thinned skull and cranial window approaches to study CNS immunobiology in the mouse cortex. 活体薄颅骨与颅窗入路研究小鼠皮层中枢神经系统免疫生物学的比较。

IntraVital Pub Date : 2014-05-01 DOI: 10.4161/intv.29728

R Dixon Dorand, Deborah S Barkauskas, Teresa A Evans, Agne Petrosiute, Alex Y Huang

引用次数: 77

Clinical applications of optical coherence tomography in urology. 光学相干断层扫描在泌尿外科的临床应用。

IntraVital Pub Date : 2014-04-30 eCollection Date: 2014-01-01 DOI: 10.4161/intv.28770

Hsing-Wen Wang, Yu Chen

引用次数: 12

Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy. 找到底部并使用它:用双光子显微镜检测体内低强度值的偏移量和灵敏度。

IntraVital Pub Date : 2014-03-01 DOI: 10.4161/intv.23674

Ruben M Sandoval, Exing Wang, Bruce A Molitoris

{"title":"Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy.","authors":"Ruben M Sandoval, Exing Wang, Bruce A Molitoris","doi":"10.4161/intv.23674","DOIUrl":"https://doi.org/10.4161/intv.23674","url":null,"abstract":"Maximizing 2-photon parameters used in acquiring images for quantitative intravital microscopy, especially when high sensitivity is required, remains an open area of investigation. Here we present data on correctly setting the black level of the photomultiplier tube amplifier by adjusting the offset to allow for accurate quantitation of low intensity processes. When the black level is set too high some low intensity pixel values become zero and a nonlinear degradation in sensitivity occurs rendering otherwise quantifiable low intensity values virtually undetectable. Initial studies using a series of increasing offsets for a sequence of concentrations of fluorescent albumin in vitro revealed a loss of sensitivity for higher offsets at lower albumin concentrations. A similar decrease in sensitivity, and therefore the ability to correctly determine the glomerular permeability coefficient of albumin, occurred in vivo at higher offset. Finding the offset that yields accurate and linear data are essential for quantitative analysis when high sensitivity is required.","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/intv.23674","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32744291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Optimization of the dorsal skinfold window chamber model and multi-parametric characterization of tumor-associated vasculature. 背侧皮肤褶窗室模型的优化及肿瘤相关血管的多参数表征。

IntraVital Pub Date : 2014-02-04 eCollection Date: 2014-01-01 DOI: 10.4161/intv.27935

Azusa Maeda, Ralph S DaCosta

引用次数: 22

IMART software for correction of motion artifacts in images collected in intravital microscopy. 用于校正活体显微术中采集的图像中的运动伪影的IMART软件。

IntraVital Pub Date : 2014-01-01 DOI: 10.4161/intv.28210

Kenneth W Dunn, Kevin S Lorenz, Paul Salama, Edward J Delp

引用次数: 23

Semi-automated vitality analysis of human trabecular meshwork 人体小梁网半自动化活力分析

IntraVital Pub Date : 2013-07-22 DOI: 10.4161/intv.27390

José M. González, James C. H. Tan

引用次数: 5

What makes a model system great? 是什么让一个模型系统变得伟大?

IntraVital Pub Date : 2013-07-22 DOI: 10.4161/intv.26287

Andrius Masedunskas, Mark A Appaduray, E. Hardeman, P. Gunning

引用次数: 2

Automated analysis of clonal cancer cells by intravital imaging. 活体成像技术对克隆癌细胞的自动分析。

IntraVital Pub Date : 2013-07-01 DOI: 10.4161/intv.26138

Sarah Earley Coffey, Randy J Giedt, Ralph Weissleder

引用次数: 18

Comprehensive assessment of quantum dots for multispectral twophoton imaging of dynamic leukocyte migration in lymph nodes 量子点对淋巴结中动态白细胞迁移的多光谱双光子成像的综合评价

IntraVital Pub Date : 2013-04-01 DOI: 10.4161/intv.25745

D. Natale, S. Soriano, F. Coelho, Miroslav Hons, J. Stein

{"title":"Comprehensive assessment of quantum dots for multispectral twophoton imaging of dynamic leukocyte migration in lymph nodes","authors":"D. Natale, S. Soriano, F. Coelho, Miroslav Hons, J. Stein","doi":"10.4161/intv.25745","DOIUrl":"https://doi.org/10.4161/intv.25745","url":null,"abstract":"In recent years, intravital twophoton microscopy (2PM) has emerged as the appropriate technique for direct in situ imaging of immune cell dynamics inside peripheral lymph nodes (PLNs) of live, anesthetized mice, yielding important insights into the regulation of immune responses. However, most current 2PM approaches are limited by the scarce availability of near-infrared (NIR) probes for multispectral time-lapse imaging, and by the use of a single excitation wavelength for multiple fluorophores. The recent availability of quantum dots (QDs) nanoparticles displaying unique optical properties have the potential to overcome this limitation but their suitability has not been yet comprehensively tested for 2PM imaging in vivo. In this study, we explored the use and delivery of NIR-emitting QDs into dendritic cells. Furthermore, we functionalized the surface of these nanoparticles with antibodies that recognize specific antigens expressed on the endothelium of the PLN microvasculature or their use as NIR plasma markers and examined the homeostatic recirculation of lymphocytes. This approach allowed to simultaneously visualize up to six different cell populations and lymphoid structures and identified varying lymphocyte migration patterns in defined microenvironments. Yet, QDs were more difficult to reproducibly couple to antibodies and showed a tendency to cause clustering of targeted antigens. Our data provide an in-depth analysis of the usefulness and shortcomings of QDs as imaging tools for anatomical landmarking in 2PM studies.","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90247072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Intravital multiphoton imaging reveals multicellular streaming as a crucial component of in vivo cell migration in human breast tumors. 活体多光子成像揭示了多细胞流是人乳腺肿瘤体内细胞迁移的重要组成部分。

IntraVital Pub Date : 2013-04-01 DOI: 10.4161/intv.25294

Antonia Patsialou, Jose Javier Bravo-Cordero, Yarong Wang, David Entenberg, Huiping Liu, Michael Clarke, John S Condeelis

{"title":"Intravital multiphoton imaging reveals multicellular streaming as a crucial component of in vivo cell migration in human breast tumors.","authors":"Antonia Patsialou, Jose Javier Bravo-Cordero, Yarong Wang, David Entenberg, Huiping Liu, Michael Clarke, John S Condeelis","doi":"10.4161/intv.25294","DOIUrl":"https://doi.org/10.4161/intv.25294","url":null,"abstract":"Metastasis is the main cause of death in breast cancer patients. Cell migration is an essential component of almost every step of the metastatic cascade, especially the early step of invasion inside the primary tumor. In this report, we have used intravital multiphoton microscopy to visualize the different migration patterns of human breast tumor cells in live primary tumors. We used xenograft tumors of MDA-MB-231 cells as well as a low passage xenograft tumor from orthotopically injected patient-derived breast tumor cells. Direct visualization of human tumor cells in vivo shows two patterns of high-speed migration inside primary tumors: (1) single cells and (2) multicellular streams (i.e., cells following each other in a single file but without cohesive cell junctions). Critically, we found that only streaming and not random migration of single cells was significantly correlated with proximity to vessels, with intravasation and with numbers of elevated circulating tumor cells in the bloodstream. Finally, although the two human tumors were derived from diverse genetic backgrounds, we found that their migratory tumor cells exhibited coordinated gene expression changes that led to the same end-phenotype of enhanced migration involving activating actin polymerization and myosin contraction. Our data are the first direct visualization and assessment of in vivo migration within a live patient-derived breast xenograft tumor.","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"2 2","pages":"e25294"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/intv.25294","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32497095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 139