Invasion & metastasis最新文献_第9页

Novel screening technique for dissemination potential of ovarian cancer cells to peritoneum. 卵巢癌细胞向腹膜播散潜力的新筛查技术。

Invasion & metastasis Pub Date : 1996-01-01

J Fujimoto, M Hori, S Ichigo, S Morishita, T Tamaya

引用次数: 0

Node-positive breast cancer: axillary micrometastases, their incidence and some implications. 淋巴结阳性乳腺癌:腋窝微转移，其发病率和一些启示。

Invasion & metastasis Pub Date : 1996-01-01

F Hartveit

引用次数: 0

Growth factor-induced epidermal invasion of the dermis in human skin organ culture: expression and role of matrix metalloproteinases. 生长因子诱导的真皮侵袭:基质金属蛋白酶的表达和作用。

Invasion & metastasis Pub Date : 1996-01-01

M E Zeigler, N T Dutcheshen, D F Gibbs, J Varani

{"title":"Growth factor-induced epidermal invasion of the dermis in human skin organ culture: expression and role of matrix metalloproteinases.","authors":"M E Zeigler, N T Dutcheshen, D F Gibbs, J Varani","doi":"","DOIUrl":"","url":null,"abstract":"Matrix metalloproteinase activity was assessed in culture fluids of organ-cultured human skin by gelatin zymography. Both the 92-kD gelatinase/type IV collagenase and the 72-kD gelatinase/type IV collagenase were detected. Production of the 92-kD enzyme was substantially increased in the presence of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) as compared to control but not in the presence of insulin-like growth factor-1 (IGF-1) or keratinocyte growth factor (KGF). This is of interest because our recent studies have shown that EGF and HGF induce the epithelial cells to invade the underlying stroma while normal architecture is maintained in the presence of IGF-1 and KGF. Addition of tissue inhibitor of metalloproteinase-2 to the organ culture fluids blocked expression of the active forms of both enzymes and concomitantly blocked invasion. Epidermal keratinocytes, dermal fibroblasts and dermal endothelial cells were grown in monolayer culture and examined for matrix metalloproteinase production. The 92-kD enzyme accounted for most of the gelatinase activity in keratinocyte culture fluids while the 72-kD enzyme accounted for most of the activity in the dermal fibroblast and endothelial cell culture fluids. Increased production of the 92-kD enzyme was seen in keratinocytes upon exposure to the growth factors that induced invasion (EGF and HGF) while the two factors that did not induce invasion (IGF-1 and KGF) were much less effective. Production of the 72-kD enzyme in fibroblasts and endothelial cells was not upregulated by any of the four growth factors. Taken together, these data indicate that matrix metalloproteinase activity is increased in the epithelium under the influence of invasion-inducing growth factors and contributes to invasion.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"16 1","pages":"11-8"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19799074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Verapamil inhibits to different extents agonist-induced Ca2+ transients in human tumor cells and in vitro tumor cell growth. 维拉帕米在人肿瘤细胞和体外肿瘤细胞生长中不同程度地抑制激动剂诱导的Ca2+瞬态。

Invasion & metastasis Pub Date : 1996-01-01

A Brocchieri, A Saporiti, M Moroni, C Porta, A Tua, G Grignani

{"title":"Verapamil inhibits to different extents agonist-induced Ca2+ transients in human tumor cells and in vitro tumor cell growth.","authors":"A Brocchieri, A Saporiti, M Moroni, C Porta, A Tua, G Grignani","doi":"","DOIUrl":"","url":null,"abstract":"Platelet agonists are known to contribute to the regulation of cytoplasmic Ca2+ levels in tumor cells and this property could be relevant in the stimulation of cell proliferation. In the present study we investigated the ability of ADP, collagen and thrombin to increase cytoplasmic Ca2+ levels in different human tumor cell lines (mesothelioma, DND-1A melanoma, HeLa uterine carcinoma) and we analyzed the effect of the calcium channel blocker verapamil on Ca2+ fluxes and on in vitro tumor cell growth. ADP was able to induce a transient increase in the cytoplasmic Ca2+ concentration in tumor cells from all lines; collagen showed this effect in mesothelioma cells and in HeLa cells, and thrombin was effective only in mesothelioma cells. Verapamil inhibited Ca2+ fluxes induced by the effective agonists in a dose-dependent manner. Values of IC50 for inhibition of ADP-induced Ca2+ transients were 63.5 microM in mesothelioma cells, 97.3 microM in DND-1A cells and 93.5 microM in HeLa cells, while those for inhibition of collagen-induced Ca2+ movements were slightly higher (170.2 microM in mesothelioma cells and 112.3 microM in HeLa cells) and the value of IC50 for inhibition of thrombin-induced Ca2+ fluxes (evaluated only in mesothelioma cells) was lower (22.5 microM). The drug dose-dependently also inhibited the in vitro growth of tumor cells; values of IC50 for growth inhibition were 21.8 microM in mesothelioma cells, 9.1 microM in DND-1A cells and 6.4 microM in HeLa cells, suggesting that the antiproliferative activity of verapamil was partly Ca(2+)-independent. These data may be of interest to elucidate the mechanisms of the two-way interactions of tumors with the hemostatic system and may help to identify new pharmacologic strategies for their control.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"16 2","pages":"56-64"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19989899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin D3 and ceramide reduce the invasion of tumor cells through extracellular matrix components by elevating protein phosphatase-2A. 维生素D3和神经酰胺通过提高蛋白磷酸酶2a，通过细胞外基质成分减少肿瘤细胞的侵袭。

Invasion & metastasis Pub Date : 1996-01-01

R J Metz, K Vellody, S Patel, R Bergstrom, J Meisinger, J Jackson, M A Wright, M R Young

{"title":"Vitamin D3 and ceramide reduce the invasion of tumor cells through extracellular matrix components by elevating protein phosphatase-2A.","authors":"R J Metz, K Vellody, S Patel, R Bergstrom, J Meisinger, J Jackson, M A Wright, M R Young","doi":"","DOIUrl":"","url":null,"abstract":"Increasing phosphorylation reactions by protein kinase A (PKA) or reducing dephosphorylation reactions of protein phosphatase-2A (PP-2A) increases the invasiveness of Lewis lung carcinoma (LLC) cells, as measured by their capacity to traverse extracellular matrix (ECM)-coated filters. Metastatic LLC-LN7 variants have reduced PP-2A activity when compared to nonmetastatic LLC-C8 variants. Immunoblotting showed that this reduced level of PP-2A activity was not due to reduced levels of the PP-2A catalytic (C) subunit. The cellular PP-2A activity could be stimulated by addition of C2-ceramide to LLC-LN7 lysates, or by incubating cells with either C2-ceramide or with a noncalcemic analog of vitamin D3, which has previously been shown to stimulate the release of ceramide. These treatments to elevate PP-2A activity in metastatic LLC-LN7 cells resulted in a decline in their capacity to invade through select (ECM) components, particularly through vitronectin and laminin. Underscoring the importance of PP-2A in limiting the invasiveness of tumor cells was the demonstration that LLC-LN7 cell transfectants overexpressing the PP-2A C alpha subunit were less invasive through ECM components than the wild-type cells. Invasion by these cells was further reduced by additionally increasing PP-2A activity by incubation with C2-ceramide or the vitamin D3 analog. These results suggest a role of a vitamin D3/ceramide/PP-2A pathway in limiting the invasiveness of tumor cells through select ECM components.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"16 6","pages":"280-90"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20301703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mathematical modelling, simulation and prediction of tumour-induced angiogenesis. 肿瘤诱导血管生成的数学建模、模拟和预测。

Invasion & metastasis Pub Date : 1996-01-01

M A Chaplain, A R Anderson

{"title":"Mathematical modelling, simulation and prediction of tumour-induced angiogenesis.","authors":"M A Chaplain, A R Anderson","doi":"","DOIUrl":"","url":null,"abstract":"Angiogenesis, the formation of blood vessels from a pre-existing vasculature, is a process whereby capillary sprouts are formed in response to externally supplied chemical stimuli. The sprouts then grow and develop, driven by endothelial cell migration and proliferation, and organise themselves into a dendritic structure. Angiogenesis occurs during embryogenesis, wound healing, arthritis and during the growth of solid tumours. In this paper we present a novel mathematical model which describes the formation of the capillary sprout network in response to chemical stimuli (tumour angiogenesis factors, TAF) supplied by a solid tumour. The model also takes into account endothelial cell-extracellular matrix interactions via the inclusion of fibronectin in the model. The model consists of a system of nonlinear partial differential equations describing the response in space and time of endothelial cells to the TAF and the fibronectin (migration, proliferation, anastomosis, branching). Using the discretized system of partial differential equations, we use a deterministic cellular automata (DCA) model, which enables us to track individual endothelial cells and incorporate branching explicity into the model. Numerical simulations are presented which are in very good qualitative agreement with experimental observations. Certain experiments are suggested which could be used to test the hypotheses of the model and various extensions and developments of the model with particular applications to anti-angiogenesis strategies are discussed.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"16 4-5","pages":"222-34"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20249795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of anti-intercellular adhesion molecule-1 and anti-lymphocyte-function-associated antigen-1 monoclonal antibodies on the metastasis of murine tumors. 抗细胞间粘附分子-1和抗淋巴细胞功能相关抗原-1单克隆抗体对小鼠肿瘤转移的影响。

Invasion & metastasis Pub Date : 1996-01-01

R Sun, H Hojo, K Kato, Y Hashimoto

引用次数: 0

Urokinase plasminogen activator is necessary but not sufficient for prostate cancer cell invasion. 尿激酶纤溶酶原激活剂对前列腺癌细胞的侵袭是必要的，但不是充分的。

Invasion & metastasis Pub Date : 1995-01-01

D F Jarrard, N M Hansen, B Patai, D B Rukstalis

引用次数: 0

Taxol reduces circulating tumor cells to prevent bone metastases in SCID mice. 紫杉醇减少循环肿瘤细胞以防止SCID小鼠骨转移。

Invasion & metastasis Pub Date : 1995-01-01

M E Stearns

{"title":"Taxol reduces circulating tumor cells to prevent bone metastases in SCID mice.","authors":"M E Stearns","doi":"","DOIUrl":"","url":null,"abstract":"The correlative effects of taxol on the reduction of circulating PC-3 ML human prostatic tumor cells and bone metastasis have been examined in SCID mice. Normally, following injection of 2 x 10(5) cells i.v., the circulating levels in peripheral blood drop by about 50 and 100%, after 8 and 24 h, respectively. In contrast, in taxol-treated mice (40-60 mg/m2/injection given 0, 3, 7 and 23 h following injection of the cells) the numbers of circulating human prostatic PC-3 ML tumor cells were reduced by 100% at 8 h. In similar experiments were mice were injected with taxol 2 h prior to injecting the cells, dosages of 40 and 60 mg/m2/injection reduced circulating tumor cells about 91 and 100%, respectively, by 8 h. Alternatively, if PC-3 Ml cells were pretreated with taxol (0.5 and 1.0 microM for 8 and 24 h) prior to injection, tumor cell clearance by 7 h was also significantly increased (80-100%). Correlative studies showed that the incidence of bone metastases (observed after 40 days) was reduced significantly (a) in mice treated with 40 and 60 mg/m2/injection (i.e. from 73-80% in controls to 15-0% in treated mice) and (b) in mice injected with PC-3 ML cells pre-exposed to 0.5-1.0 microM taxol for 7 h. Immunofluorescence studies with tubulin antibodies showed that the microtubules were disrupted in cells exposed to taxol in vivo and in vitro under conditions that significantly increased cellular clearance from the blood. Taken together, the data suggests that taxol at nontoxic dosages (to mice) can prevent metastases by directly reducing the circulating levels of tumor cells.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"15 5-6","pages":"232-41"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19736601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glioma cell invasion visualized by scanning confocal laser microscopy in an in vitro co-culture system. 在体外共培养系统中扫描共聚焦激光显微镜观察胶质瘤细胞侵袭。

Invasion & metastasis Pub Date : 1995-01-01

S J Nygaard, P H Pedersen, T Mikkelsen, A J Terzis, O B Tysnes, R Bjerkvig

{"title":"Glioma cell invasion visualized by scanning confocal laser microscopy in an in vitro co-culture system.","authors":"S J Nygaard, P H Pedersen, T Mikkelsen, A J Terzis, O B Tysnes, R Bjerkvig","doi":"","DOIUrl":"","url":null,"abstract":"Confrontation cultures between glioma spheroids and brain cell aggregates are well established in glioma research, and the model reflects several similarities to the in vivo brain tumour invasive process. The lipid-binding fluorescent carbocyanine dyes DiO (3,3'-dioctadecyloxacarbocyanine perchlorate) and DiI (1,1'-dioctadecyl-3,3,3,'3,'-tetramethylinocarbocyanine perchlorate) are widely used in cell biology as tracers for studying cell movement. Mature brain cell aggregates grown from fetal rat brain cells, and spheroids initiated from two glioma cell lines (GaMg and D-54Mg) were stained with DiO and DiI, respectively. Penetration of DiI and DiO into the tumour spheroids and brain aggregates was studied by confocal laser scanning microscopy (CLSM). After 48 h of dye exposures, the tracers had almost completely penetrated the tumour spheroids and brain aggregates. Light-microscopic sections of the specimens indicated that the dye incorporation had little effect on cellular morphology. Cell migration from DiI stained D-54Mg and GaMg spheroids was similar to that observed from unstained spheroids. Growth was also unaffected after 48 h of DiI exposure. Gioma cell invasion was assessed by CLSM using co-cultures of DiI -stained spheroids and DiO-stained brain cell aggregates. Optical sections revealed a gradual decrease in remaining brain volume, indicating a progressive invasive process. Single tumour cells were identified deep within the brain aggregates. In addition normal brain cells were also identified in the tumour spheroids. It is concluded that vital staining can be used to identify both normal cells and tumour cells during tumour cell invasion in vitro. The method may provide the possibility for studying the kinetics of single normal and tumour cell movement in individual tumour/brain co-cultures.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"15 5-6","pages":"179-88"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19737852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0