International Journal of Secondary Metabolite最新文献_第9页

Mineral contents, antimicrobial profile, acute and chronic toxicity of the aqueous extract of Moroccan Thymus vulgaris in rodents 摩洛哥百里香水提取物的矿物质含量、抗菌特性、对啮齿类动物的急性和慢性毒性

International Journal of Secondary Metabolite Pub Date : 2022-12-10 DOI: 10.21448/ijsm.1106820

Nidal Naceiri Mrabti, H. N. Mrabti, L. Doudach, Zineb Khalil, M. R. Kachmar, Mouna Mekkaoui, M. Faouzi, E. Abdallah, G. Zengin, A. Bouyahya, M. Elhallaoui

{"title":"Mineral contents, antimicrobial profile, acute and chronic toxicity of the aqueous extract of Moroccan Thymus vulgaris in rodents","authors":"Nidal Naceiri Mrabti, H. N. Mrabti, L. Doudach, Zineb Khalil, M. R. Kachmar, Mouna Mekkaoui, M. Faouzi, E. Abdallah, G. Zengin, A. Bouyahya, M. Elhallaoui","doi":"10.21448/ijsm.1106820","DOIUrl":"https://doi.org/10.21448/ijsm.1106820","url":null,"abstract":"Moroccan flora is rich in plants used in traditional medicine, but the further scientific investigation is necessary. The aim of the research was to evaluate the nutritional content and antimicrobial activity of Moroccan Thymus vulgaris, as well as its possible acute and chronic toxicological effects on rodents. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) was used to determine the mineral content. The antimicrobial activity was determined using a well-diffusion test, a minimum inhibitory concentration (MIC), and a minimum bactericidal/fungicidal concentration (MBC/MFC) assay. Acute and chronic toxicity studies were conducted in vivo on mice and rats, respectively. Following that, haematological, serum-biochemistry, and histological investigations were performed. Moroccan Thyme was shown to be a source of numerous minerals which are necessary for health promotion. All antimicrobial testing, disc diffusion, MIC, and MBC tests revealed that thyme had potent antibacterial activity against all microorganisms tested. Staphylococcus aureus was the most susceptible bacterium, followed by Salmonella enterica and Escherichia coli. Additionally, thyme exhibited great antifungal efficacy against Candida albicans. The acute toxicity results indicated that the aqueous extract of T. vulgaris is almost non-toxic when taken orally. According to the chronic toxicity study, the extract is generally safe when taken orally over an extended period of time. The biochemical and haematological characteristics of the serum and blood were within acceptable limits, and histological examination revealed no abnormalities. In conclusion, the findings of this investigation, confirm the antimicrobial efficacy of the aqueous extract of Moroccan T. vulgaris and its safety for experimental animals.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46956113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review on phytochemicals and biological activities of natural sweeteners Stevia rebaudiana Bertoni 天然甜味剂甜菊糖的植物化学成分及生物活性研究进展

International Journal of Secondary Metabolite Pub Date : 2022-12-10 DOI: 10.21448/ijsm.1122618

M. Hossain, Mr. Said AL HARTHY, Dr. Salem SAİD AL TOUBY

{"title":"Review on phytochemicals and biological activities of natural sweeteners Stevia rebaudiana Bertoni","authors":"M. Hossain, Mr. Said AL HARTHY, Dr. Salem SAİD AL TOUBY","doi":"10.21448/ijsm.1122618","DOIUrl":"https://doi.org/10.21448/ijsm.1122618","url":null,"abstract":"Diabetes is a chronic metabolic disease that creates high blood sugar level. Therefore, diabetes awareness is necessary to prevent diabetes by reducing sugar intake and using low-calorie alternative sweeteners instead. Stevia rebaudiana is a medicinal plant species belonging to the Compositae family. It is a sweet herb that contains diterpene glycosides, which are directly responsible for the sweet taste, but they have no caloric value. Since ancient times, there have been several reports on the use of S. rebaudiana as an alternative sweetener and extended research has been conducted on its phytochemicals and biological activities. The plant contains a good number of phytochemicals with significant biological activities, namely polyphenolic derivatives, diterpenes glycosides, alkaloids, glycosides, tannins, chlorophylls, carotenoids, etc. For industrial use, those phytochemicals could be extracted from the selected plant and used for the preparation of nutraceuticals and food additives. S. rebaudiana is a natural herb; therefore, it has fewer or minimal adverse effects on human health. The selected plant in various forms is used for the treatment of diabetes, colon cancer, obesity, cavities, and others. However, the literature review shows that the information on this plant and its uses is not systematic. The purpose of the present review is to explore the status of phytochemicals and biological activities of the selected plant for young researchers. Therefore, the updated data will help them to develop new nutraceuticals and food additives that could help in the production of pharmaceuticals to treat different ailments.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43829926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical profiling of Oxalis species growing wild in Egypt using HRLC/MS Spectrometry HRLC/MS法分析埃及野生草叶草的化学特征

International Journal of Secondary Metabolite Pub Date : 2022-12-10 DOI: 10.21448/ijsm.1110807

A. Draz, S. Kawashty, E. Shamso, H. Hosni, S. Hussein

{"title":"Chemical profiling of Oxalis species growing wild in Egypt using HRLC/MS Spectrometry","authors":"A. Draz, S. Kawashty, E. Shamso, H. Hosni, S. Hussein","doi":"10.21448/ijsm.1110807","DOIUrl":"https://doi.org/10.21448/ijsm.1110807","url":null,"abstract":"Diabetes is a chronic metabolic disease that creates high blood sugar level. Therefore, diabetes awareness is necessary to prevent diabetes by reducing sugar intake and using low-calorie alternative sweeteners instead. Stevia rebaudiana is a medicinal plant species belonging to the Compositae family. It is a sweet herb that contains diterpene glycosides, which are directly responsible for the sweet taste, but they have no caloric value. Since ancient times, there have been several reports on the use of S. rebaudiana as an alternative sweetener and extended research has been conducted on its phytochemicals and biological activities. The plant contains a good number of phytochemicals with significant biological activities, namely polyphenolic derivatives, diterpenes glycosides, alkaloids, glycosides, tannins, chlorophylls, carotenoids, etc. For industrial use, those phytochemicals could be extracted from the selected plant and used for the preparation of nutraceuticals and food additives. S. rebaudiana is a natural herb; therefore, it has fewer or minimal adverse effects on human health. The selected plant in various forms is used for the treatment of diabetes, colon cancer, obesity, cavities, and others. However, the literature review shows that the information on this plant and its uses is not systematic. The purpose of the present review is to explore the status of phytochemicals and biological activities of the selected plant for young researchers. Therefore, the updated data will help them to develop new nutraceuticals and food additives that could help in the production of pharmaceuticals to treat different ailments.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47540921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Composition analysis and antibacterial activity evaluation of different crude extracts of Mentha piperita (Lamiaceae) 薄荷科不同粗提物的成分分析及抑菌活性评价

International Journal of Secondary Metabolite Pub Date : 2022-12-10 DOI: 10.21448/ijsm.1079952

Ms. Zahra Mohammed AL-HAJRİ, M. Hossain, Salim Said AL-TOUBY2

{"title":"Composition analysis and antibacterial activity evaluation of different crude extracts of Mentha piperita (Lamiaceae)","authors":"Ms. Zahra Mohammed AL-HAJRİ, M. Hossain, Salim Said AL-TOUBY2","doi":"10.21448/ijsm.1079952","DOIUrl":"https://doi.org/10.21448/ijsm.1079952","url":null,"abstract":"Globally, the main target of scientists is to examine new medicinally beneficial plants for the preparation of herbal drugs as well as to explore possible uses in the food industry. In this regard, the target of our current study is to evaluate the antibacterial activity and biochemical analyses of the leaf extracts of Omani Mentha piperita L (M. piperita). The selected plant leaves coarse powder samples were extracted by using the Soxhlet extraction process with methanol at 65oC for a period of 72 hours. The evaporation of methanol was done by a rotary evaporator under reduced pressure and temperature. The hydro alcoholic extract was fractionated with the various polarities of solvents with an increasing pattern. The biochemical evaluation and pharmacological activity of the prepared local plant extracts were completed by using established methods. The different polarities of leaves extracts showed positive biochemical tests of alkaloids, flavonoids, glycosides, saponins, steroids, tannins and triterpenoids. The microbial activity of the fractioned plant extracts was tested with improved agar gel method. The different polarity extracts at different concentrations did not display any activity against the tested Gram (+) Staphylococcus aureus (S. aureus) and Gram (-) Escherichia coli (E. coli) and Pseudomonus aeruginosa (P. aeruginosa) bacterial strains. Based on the biochemical and pharmacological evaluation results, the selected whole plant and its potential extracts might be used traditionally as natural antibiotics to treat infectious diseases.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43542113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of glutathione on mitochondrial DNA and antioxidant enzyme activities in Drosophila melanogaster 谷胱甘肽对黑腹果蝇线粒体DNA和抗氧化酶活性的影响

International Journal of Secondary Metabolite Pub Date : 2022-12-10 DOI: 10.21448/ijsm.1084592

Hülya Yıldız

{"title":"Effects of glutathione on mitochondrial DNA and antioxidant enzyme activities in Drosophila melanogaster","authors":"Hülya Yıldız","doi":"10.21448/ijsm.1084592","DOIUrl":"https://doi.org/10.21448/ijsm.1084592","url":null,"abstract":"The free radical theory in aging assumes that the accumulation of macromolecular damage induced by toxic reactive oxygen species plays a central role in the aging process. The intake of nutritional antioxidants can prevent this damage by neutralizing reactive oxygen derivatives. Glutathione (GSH; en-L-Glutamyl-L-cysteinyl glycine) is the lowest molecular weight thiol in the cells and as a cofactor of many enzymes and a potent antioxidant plays an important role in maintaining normal cell functions by destroying toxic oxygen radicals. In this study, the effects of GSH on SOD, GST and catalase enzymes and mtDNA damage were investigated at various time intervals by giving reduced glutathione to Drosophila. It was observed that 3-week GSH administration did not have a statistically significant effect on SOD and GST activities whereas GSH application decreased the catalase enzyme activities significantly. Although the decrease in antioxidant capacity with age was observed in SOD and catalase enzymes, such a situation was not observed in GST enzyme activities. There was no statistically significant difference between the control and GSH groups in mtDNA copy number values, while in the GSH group, oxidative mtDNA damage was high. These results may be due to the prooxidant effect of GSH at the dose used in this study.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47517417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of drying method and infusion time on purple basil leaves tea 干燥方法和浸泡时间对紫苏叶茶的影响

International Journal of Secondary Metabolite Pub Date : 2022-11-29 DOI: 10.21448/ijsm.1116574

Ayca Gülhan, H. Çoklar, M. Akbulut

引用次数: 1

Sium sisarum Sium sistemum

International Journal of Secondary Metabolite Pub Date : 2022-09-01 DOI: 10.21448/ijsm.1066904

Muzaffer Mükemre, Abdullah Dalar, Şengal BAĞCİ TAYLAN, Metin Ertaş

引用次数: 1

An application of CoFe2O4/alginate magnetic beads: drug delivery system of 5-fluorouracil CoFe2O4/海藻酸磁珠在5-氟尿嘧啶给药系统中的应用

International Journal of Secondary Metabolite Pub Date : 2022-09-01 DOI: 10.21448/ijsm.1052662

A. Yıldırım, Yasemin İspirli Doğaç

{"title":"An application of CoFe2O4/alginate magnetic beads: drug delivery system of 5-fluorouracil","authors":"A. Yıldırım, Yasemin İspirli Doğaç","doi":"10.21448/ijsm.1052662","DOIUrl":"https://doi.org/10.21448/ijsm.1052662","url":null,"abstract":"Magnetic hyperthermia therapy is expected to play an important role in the treatment of more and more cancers. The synergistic effects of using together hyperthermia and cancer drugs have been shown by literature studies to be more effective than either hyperthermia treatment alone or chemotherapy alone. In addition, magnetic materials that can be used as a contrast agent enable magnetic resonance imaging of the tumor, which is also useful in seeing the treatment progress. This study, which was designed for this purpose, occurred in three parts: In the first part, magnetic CoFe2O4/alginate composite beads were prepared and characterized with thermogravimetric analysis (TGA) and scanning electron microscope (SEM). In the second part, the swelling behaviour of magnetic composite beads was investigated at pH 1.2, pH 7.4 and pH 6.8. It was seen that at pH 7.4 and pH 6.8, that is, near neutral pH, CFA swelled by 81.54% and 82.69%, respectively. In the third part, 5-Fluorouracil was encapsulated at the different ratios in CoFe2O4/alginate composite beads, and release experiments were performed at pH 1.2, pH 7.4 and pH 6.8. 5-FU release was calculated with Korsmeyer-Peppas, Higuchi, first-order, and zero-order models. It was seen that the drug release systems prepared were suitable for all kinetic models. Magnetic CoFe2O4/alginate composite bead, which is the drug carrier, was determined to be suitable for controlled release for 5-Fluorouracil.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47651674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Chemical Composition of the Essential Oil of Thymus longicaulis C. Presl. subsp. longicaulis 麝香精油的化学成分研究。无性系种群。longicaulis

International Journal of Secondary Metabolite Pub Date : 2022-09-01 DOI: 10.21448/ijsm.1086904

Ömer Elkıran

引用次数: 0

Anti-quorum sensing and cytotoxic activity of elemi essential oil elemi精油的抗群体感应和细胞毒性活性

International Journal of Secondary Metabolite Pub Date : 2022-09-01 DOI: 10.21448/ijsm.1059886

A. Soyocak, A. Ak, E. Önem

{"title":"Anti-quorum sensing and cytotoxic activity of elemi essential oil","authors":"A. Soyocak, A. Ak, E. Önem","doi":"10.21448/ijsm.1059886","DOIUrl":"https://doi.org/10.21448/ijsm.1059886","url":null,"abstract":"Essential oils have several biological activities such as antimicrobial, antioxidant, proliferative, and anti-inflammatory. This study aimed identification of bioactive compounds found in Elemi essential oil (EO) and to determine the anti-quorum sensing and cytotoxic activities of EO. In this study, bioactive compounds of EO were analyzed using GC-MS, and the antibacterial activity of elemi was screened against Staphylococcus aureus ATCC 25923, Methicillin-Resistant Staphylococcus aureus ATCC 43300, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PAO1. Anti-biofilm activity and pyocyanin production on P. aeruginosa PAO1 were also investigated. The effect of EO on cell viability was also analyzed by thiazolyl blue tetrazolium bromide (MTT) and neutral red uptake (NR) assay in fibroblast cells. According to GC results, the major component of EO was determined as limonene (55%). A sub-MIC of elemi essential oil inhibited biofilm formation and pyocyanin production by 43% and 56%, respectively. On the other hand, EO also had an acute effect on the mitochondrial and lysosomal activities of fibroblast cell lines. Mitochondrial and lysosomal activities were significantly decreased when EO concentrations were applied for 24 and 48 hours (p<0.05). In conclusion, EO has inhibitory activity on biofilm formation and pyocyanin production, and also the lower doses of oil have no toxic effects on fibroblast cells. However, higher doses of EO have more cytotoxic effects on mitochondrial activity rather than the lysosomal activity of fibroblast cell lines. It is thought that EO exhibits these activities due to the amount of limonene in its content. ","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41428893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1