International Journal of Veterinary Medicine最新文献

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Changes in the gas composition of umbilical cord blood of newborns after cesarean section using various protocols of general anesthesia in dogs 在狗身上使用不同的全身麻醉方案进行剖腹产后新生儿脐带血气体成分的变化
International Journal of Veterinary Medicine Pub Date : 2023-12-18 DOI: 10.52419/issn2072-2419.2023.4.480
K. P. Sadovedov, A. Nechaev, K. Plemyashov
{"title":"Changes in the gas composition of umbilical cord blood of newborns after cesarean section using various protocols of general anesthesia in dogs","authors":"K. P. Sadovedov, A. Nechaev, K. Plemyashov","doi":"10.52419/issn2072-2419.2023.4.480","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.480","url":null,"abstract":"Cesarean section, being one of the most common  operations  in  veterinary  surgery, requires  a  special  anesthetic  protocol  that provides adequate anesthesia for the mother and has minimal effect on puppies. The aim of  the  study  was  to  evaluate  the  effect  of various non-inhalation and inhaled anesthetics on newborn puppies during cesarean section,  based  on  the  analysis  of  cord  blood gases.  3  groups  of  experimental  animals were formed, which used one of the studied anesthetic protocols. In the first experimental group, propofol at a dose of 4-6 mg/kg was used to maintain general anesthesia. Inhaled halogen-containing anesthetics were used in the  second  and  third  experimental  groups. Isoflurane 0.5 – 2.0 vol% was  used  in  the second  group,  and  sevoflurane  1.0  –  2.0 vol%  was  used  in  the  third  group.  When using  all  the  studied  anesthetics,  an  acidbase imbalance in the form of moderate acidosis was detected in the puppies after delivery, however, acidosis was less pronounced in the groups where halogen-containing anesthetics were used. The use of inhaled halogen-containing anesthetics had a lesser effect on puppies compared to non-inhalation ones. There were no significant differences in the effect  of  isoflurane  and  sevoflurane  from inhaled anesthetics.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":" 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138963524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of nonsteroidal and steroid anti-inflammatory drugs on platelet activation IN VITRO 非类固醇和类固醇抗炎药对体外血小板活化的影响
International Journal of Veterinary Medicine Pub Date : 2023-12-18 DOI: 10.52419/issn2072-2419.2023.4.463
A. Bokarev, A. O. Minina, V. E. Gorokhov
{"title":"The effect of nonsteroidal and steroid anti-inflammatory drugs on platelet activation IN VITRO","authors":"A. Bokarev, A. O. Minina, V. E. Gorokhov","doi":"10.52419/issn2072-2419.2023.4.463","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.463","url":null,"abstract":"The article presents the results of a study on the effect of steroid and nonsteroidal anti-inflammatory drugs on platelets.The object of the study was plasma obtained  from  rat  blood  (n=10).  Blood  sampling (2.0 ml from each animal) was carried out  from  the  tail  vein  into  test  tubes  with sodium citrate. Cell elements were counted directly in blood and in platelet plasma using a MEK-6550 hematological analyzer.The  ability  to  influence  platelet  activation was determined in drugs such as dexamethasone,  ketarol  and  miloxicam,  which were injected into test tubes before plasma reactivation. It  was  found  that  the  drugs  of  these groups affect the metabolism of these cells and inhibit their ability to cause clot contraction.  It  was  found  that  the  strength  of  the negative  effect  on  clot  contraction  in  the steroid drug dexamethasone, which through intermediaries  suppresses  the  activity  of phospholipase-A2 and the nonsteroidal drug ketarol,  which  is  the  predominant  inhibitor of cyclooxygenase of the first type, are similar.  It  was  also  found  out  that  the  drug meloxicam, which is a predominant inhibitor of cyclooxygenase of the second type, causes only a slight decrease in platelet clot contraction. The results obtained in the experiment indicate  a  direct  positive  relationship  between the degree of clot contraction and the amount  of  thromboxane-A2,  which  is  synthesized and released by activated platelets. It  is  known  that  the  ability  of  platelets  to accelerate and enhance regenerative processes is also directly related to their activation. Based  on  this,  it  is  suggested  that  patients who have been  prescribed  plateletenriched plasma  therapy  should  not  use  glucocorticoids and preparations with a high degree of selectivity  to  cyclooxygenase  of  the  first type as anti-inflammatory drugs.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":" 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138995126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of metabolism and functioning of the antioxidant system in cats with tripelphosphate urolithiasis 三磷酸腺尿石症猫体内抗氧化系统的新陈代谢和功能研究
International Journal of Veterinary Medicine Pub Date : 2023-12-18 DOI: 10.52419/issn2072-2419.2023.4.515
T. Ushakova, T. N. Derezina, V. S. Chichilenko
{"title":"Investigation of metabolism and functioning of the antioxidant system in cats with tripelphosphate urolithiasis","authors":"T. Ushakova, T. N. Derezina, V. S. Chichilenko","doi":"10.52419/issn2072-2419.2023.4.515","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.515","url":null,"abstract":"Urolithiasis  is  a  chronic  disease  of  the urinary  tract  of  a  polyethological  nature, accompanied  by  the  formation  of  uroliths and sand in the kidneys and urinary tract, as well as signs of dysuria, pollakiuria, ischuria,  urinary  colic,  periodic  hematuria  and crystalluria. The purpose of our research was to study the metabolism and functioning of the  antioxidant  system  in  cats  with  tripelphosphate  urolithiasis.  The  results  of  our study of the metabolism and antioxidant system  of  sick  animals  indicated  the  development of disorders of redox regulation of cells in tripelphosphate erythrolithiasis in cats. A disorder of liver functional activity was detected  (AST  -  41.74±5.35  Units/l  and 49.30±6.74  Units/L;  ALT  - Units/l  and  Units/L;  ALKP - Units/L and  Units/L; BILT -  mmol/l  and mmol/l; BILD -  mmol/l and 1.62mmol/L;  AAR  –  0.49±0.03  and 0.54±0.02) and violation of cell redox regulation  (MDA  -  1.29±0.08  nmol/ml  and 1.40±0.10  nmol/ml;  SOD -  265.90±12.08 Units/ml and 280.05±10.43 Units/ml; coenzyme  Q10  -  579.03±19.06  ng/ml  and 505.42±23.10  ng/ml;8-OHdG -  0.15±0.01 ng/ml  and  0.13±0.02ng/ml;  ascorbic  acid - 5.03±0.91  mcg/ml  and  4.70±1.03  micrograms/ml; TAS - 1.25±0.07 mmol/l and 1.23±0.05  mmol/L;  GTP -  6234.00±24.82 Units/g Hb and 6015.12±27.56units/g Hb) in cats with tripelphosphate urolithiasis. It was found that there is a direct close correlation between the level of liver transaminases and the activity of antioxidant enzymes, as well as  between  the  level  of  circulating  kidney biomarkers and the level of the main markers of oxidative stress in urolithiasis struvite type in cats, which allows us to expand the understanding of this disease and use these data  in  the  implementation  of  therapeutic measures.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"61 S279","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138965316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
To study the anabolic aspects of lactation in cows in connection with the intensity of ketogenesis in the new body period 研究奶牛泌乳期的合成代谢与新体期酮体生成强度的关系
International Journal of Veterinary Medicine Pub Date : 2023-12-17 DOI: 10.52419/issn2072-2419.2023.4.447
S. Vasileva
{"title":"To study the anabolic aspects of lactation in cows in connection with the intensity of ketogenesis in the new body period","authors":"S. Vasileva","doi":"10.52419/issn2072-2419.2023.4.447","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.447","url":null,"abstract":"The  article  discusses  the  results  of  a study  of  the  milk  productivity  of  cows  in connection  with  the  content  of  beta-hydroxybutyric acid in the blood during the new calving period. The studies were carried out on highly productive full-aged cows of the second and third lactation. The animals were divided into three groups according to the content of beta-hydroxybutyrate - up to 1.0 mmol/l; from 1.0 to 2.0 mmol/l and over 2.0 mmol/l. It has been established that an increase  in  the  content  of  ketones  in  the blood  is  associated  with  hypoglycemia. Cows  with  increased  levels  of  beta-hydroxybutyric acid at the beginning of lactation showed higher daily milk yields over a five-month  period  of  observation.  In  the fourth and fifth months of lactation, the milk yield  of  these  cows  was  higher  than  in groups  1  and  2  by  14.6-15.8%  and  18.5-20.1%, respectively. In addition, the total yield of fat in this group is greater than in the first and second - by 12.8% and 10.7%, protein - by 9.7% and 11.2%,  and  lactose -  by  8.5%  and  13.5%, respectively.  A  calculation  indicator  was proposed -  the  lactation  anabolism  index, which  is  calculated  using  daily  milk  yield, mass fraction of milk protein and urea concentration.  An  increase  in  this  index  indicates the activation of anabolic reactions, a decrease indicates a surge in catabolic processes.  An  increase  in  the  lactation  anabolism index  was found in  cows of the third group at 4-5 months of lactation with a significant increase of 44.8-45.8% in comparison with groups 1 and 2. A high metabolic potential for further productive lactation was established in cows that had increased concentration of beta-hydroxybutyric acid in the new calving period.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"5 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The microbial landscape of the genital tract of healthy cows with different gestation periods 不同妊娠期健康奶牛生殖道的微生物景观
International Journal of Veterinary Medicine Pub Date : 2023-12-17 DOI: 10.52419/issn2072-2419.2023.4.431
P. Parshin, G. Vostroilova, Yu. N. Brigadirov, I. Shaposhnikov, M. S. Zhukov, O. Manzhurina, I. S. Perepelkina, O. V. Yakimchuk
{"title":"The microbial landscape of the genital tract of healthy cows with different gestation periods","authors":"P. Parshin, G. Vostroilova, Yu. N. Brigadirov, I. Shaposhnikov, M. S. Zhukov, O. Manzhurina, I. S. Perepelkina, O. V. Yakimchuk","doi":"10.52419/issn2072-2419.2023.4.431","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.431","url":null,"abstract":"The doctrine of the normal microflora of the human and animal body is currently in the focus of attention of clinical microbiologists and a wide range of clinical specialists. It is known that the microflora of the vagina depends on many factors of the internal and external  environment,  therefore,  it  is  now considered to be an ecosystem with a unique set of mechanisms that ensure its resistance to  foreign  microorganisms  and  support  reproductive health. The aim of the study was to study changes in vaginal microbiocenosis in cows during different periods of pregnancy.  The  object  of  the  study  was  clinically healthy cows (n=10) of a redmottled breed (holshitinized), and the subject of the study was  cervical–vaginal  mucus.  On  the  150-160, 210-220 and 260-265 days of pregnancy,  mucus  samples  were  taken  from  cows using sterile probe tampons for bacteriological studies. It was found that representatives of  the  normoflora,  as  well  as  conditionally pathogenic and pathogenic microflora were present in the vaginal secretions of animals of  all  periods  of  pregnancy.  However,  the total  bacterial  contamination  of  the  genital tract in cows in the first half and second half of the third trimester of pregnancy was characterized by its decrease by 29.2 and 33.3%, respectively, relative to the data obtained in the second trimester. It should also be noted that by the second half of the third trimester of pregnancy, there is an increase in obligate normoflora due to lacto- and bifidobacteria by 27.1% and 4 times, respectively, with the predominance of the genus Lactobacillus in quantitative terms. Against this background, the amount of transient microflora decreased by 34.2-57.1% (P0.05).","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of mesenchymal stem cells from the adipose tissue and bone marrow of goats on the quality of their sperm cells 来自山羊脂肪组织和骨髓的间充质干细胞对其精子细胞质量的影响
International Journal of Veterinary Medicine Pub Date : 2023-12-17 DOI: 10.52419/issn2072-2419.2023.4.421
E. Korochkina, A. V. Trifonova, E. Finageev, D. Glavatskaya, V. Pushkina
{"title":"The effect of mesenchymal stem cells from the adipose tissue and bone marrow of goats on the quality of their sperm cells","authors":"E. Korochkina, A. V. Trifonova, E. Finageev, D. Glavatskaya, V. Pushkina","doi":"10.52419/issn2072-2419.2023.4.421","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.421","url":null,"abstract":"The purpose of this research is to study the  influence  of  mesenchymal  stem  cells from  adipose  tissue  and  bone  marrow  of goats  on  the  quality  parameters  of  their sperm. A complex evaluation of sperm quality  (volume,  concentration,  morphology, motility) of Saanen and Czech goats (n=10) at the age of 1-2.5 years was carried out according  to  generally  accepted  methods  and protocols.  Adipose  tissue  (AT)  and  bone marrow  (BM)  were  obtained  after  the slaughter of goats (n=6) on a peasant farm in the Volkhov district of the Leningrad region. After processing the obtained samples, mesenchymal stem cells obtained from adipose tissue and bone marrow of goats were isolated,  accumulated  and  cryopreserved.  Three groups of samples of 10 each were formed: control (spermatozoa 7x1010 cells/ml + PBS buffer 100 μl, pH – 7.4), first experimental (spermatozoa  7x1010  cells/ml  +  MSCs 2x1010 cells/ml, pH – 7.2), the second was experimental (sperm 7x1010 cells/ml + BM MSCs 2x108 cells/ml, pH – 7.2) and a four-stage assessment of sperm quality was carried out: after incubation of sperm with mesenchymal  stem  cells  at  a  temperature  of 38℃ for 0, 1, 2 and 3 hours. Based on the results obtained, the working protocol for the use of mesenchymal stem cells from adipose tissue  and  bone  marrow  for  the  sperm  of breeding goats is the concentration of MSCs -  2x108  cells/ml  and  the  concentration  of goat  spermatozoa -  7x1010  cells/ml.  The studies conducted have established the positive effect of mesenchymal stem cells from the adipose tissue and bone marrow of goats on the quality indicators of their sperm. At the  same  time,  spermatozoa  remain  viable during three-hour incubation with a progression  of  movements  equal  to  51.5±8.46  - 51.8±5.2% and a number of morphologically normal  sperm  equal  to  50.40±2.21  - 51.10±3,  14%  (3  hours  after  incubation, MSCs from adipose tissue and bone marrow, respectively).","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The concentration of anti-muller hormone in the blood serum of reindeer depends on the level of sex hormones 驯鹿血清中抗穆勒激素的浓度取决于性激素水平
International Journal of Veterinary Medicine Pub Date : 2023-12-17 DOI: 10.52419/issn2072-2419.2023.4.438
A. Krutikova, E. Nikitkina, G. V. Shiriaev, G. K. Peglivanian, A. A. Musidray, G. S. Nikitin
{"title":"The concentration of anti-muller hormone in the blood serum of reindeer depends on the level of sex hormones","authors":"A. Krutikova, E. Nikitkina, G. V. Shiriaev, G. K. Peglivanian, A. A. Musidray, G. S. Nikitin","doi":"10.52419/issn2072-2419.2023.4.438","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.438","url":null,"abstract":"Reindeer  husbandry  is  the  leading branch of agriculture for the regions of the Far  North.  Reindeer  (Rangifer  tarandus) remains  poorly  studied  from  the  point  of view  of  genetics  and  reproduction,  which hinders the intensification of development of the industry. Research is being actively conduct in the field of genetics of the productive qualities of reindeer, but the formation and functioning  of  its  reproductive  system  has been little study. Hormonal regulation of the reproductive  process  and  the  formation  of hormonal status at key stages of the reproductive cycle is a significant aspect of animal reproduction. Reindeer are characterized by a pronounced seasonality of reproduction, and the study of hormonal regulation of the reproductive cycle is the main aspect of the further use of assisted reproductive technologies. The work is devoted to the analysis of the  level  of  anti-Mullerian  hormone  in  females during the breeding season. The studies were carried out on reindeer farms of the MAU “Directorate of the Territory of Traditional Natural Resources Management  of  Soyots”,  Buryatia,  (female, n=29). The dependence of the concentration of anti-Müllerian hormone (AMH), which is a generally accepted indicator of the female ovarian reserve, on the concentration of sex hormones  (progesterone,  estradiol,  testosterone and luteinizing hormone) in the blood serum of females was study. Hormone levels were  determined  by  enzyme  immunoassay. A higher content of sex hormones - testosterone,  estradiol,  progesterone  and  luteinizing  hormone  was  revealed  in  the  group  of women  with  an  AMH  concentration  of  >1 ng/ml than in the group of women with an AMH concentration of <1 ng/ml. The significance of the differences in progesterone was p<0.05,  and  in  estradiol – p<0.001.  A  low concentration  of  AMH  (<1  ng/ml),  characterizing a reduced ovarian reserve of female reindeer,  corresponds  to  a  reduced  concentration of sex hormones that support reproductive  function,  and,  conversely,  a  high concentration of AMH (>1 ng/ml), and, accordingly, a high ovarian reserve the reserve corresponds to an increased concentration of sex hormones - testosterone, estradiol, progesterone and luteinizing hormone.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"345 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The genetic basis of the sperm quality of stallions 种马精子质量的遗传基础
International Journal of Veterinary Medicine Pub Date : 2023-12-17 DOI: 10.52419/issn2072-2419.2023.4.413
E. Nikitkina
{"title":"The genetic basis of the sperm quality of stallions","authors":"E. Nikitkina","doi":"10.52419/issn2072-2419.2023.4.413","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.413","url":null,"abstract":"One of the important factors affecting the reproductive function is the genotype of the animal. The method of genomic selection is based on the use of polymorphic single nucleotide substitutions (SNP) as markers  for determining the value of the genotype of an animal or plant. The formation and functioning  of  the  reproductive  system  of  animals occurs as a result of the coordinated interaction of a wide range of genes.  The search for genomic  associations  of  GWAS  (Genome-Wide Association Studies) with signs of fertility will allow further selection of animals based  on  these  indicators  at  the  genomic level. Sperm was obtained with the help of mares in hunting and on a phantom using an artificial  Hannover  vagina  (Minitube,  Germany). The sperm of each stallion was collected at least three times. The semen quality of 96 stallions of different breeds (Arabian, Akhal-Teke, Trakenen, thoroughbred horse, Oryol  trotter,  French  trotter,  Soviet  heavy horse, Shetland pony, Welsh pony, horses of different breeds) was analyzed. High variability  of  sperm  quality  indicators  was  revealed.  For  example,  the  concentration  of sperm in the ejaculate varied from 59 to 514 million /ml, progressive mobility from 20 to 87%, and the number of cells without damage from 21.6 to 95.6%. Such variability of the studied features makes it possible to assume that their formation in the process of ontogenesis  occurs  under  the  influence  of genetic factors. The GWAS analysis of ejaculate volume, concentration and motility of stallion  sperm  revealed  potential  candidate genes associated with these indicators. The associations identified in our study are promising candidates for further identification of the  biological  mechanisms  underlying  the quality of stallion sperm.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"345 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Laboratory methods for determining the marker of proliferative activity of Ki-67 in the placenta of cows in complicated pregnancy and pathological childbirth 确定难产妊娠和病理分娩奶牛胎盘中 Ki-67 增殖活性标记的实验室方法
International Journal of Veterinary Medicine Pub Date : 2023-12-17 DOI: 10.52419/issn2072-2419.2023.4.403
V. Avdeenko, S. A. Makavchik, D. I. Safronov, K. A. Moiseeva
{"title":"Laboratory methods for determining the marker of proliferative activity of Ki-67 in the placenta of cows in complicated pregnancy and pathological childbirth","authors":"V. Avdeenko, S. A. Makavchik, D. I. Safronov, K. A. Moiseeva","doi":"10.52419/issn2072-2419.2023.4.403","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.403","url":null,"abstract":"In  modern  high-tech  dairy  enterprises, the problem of perinatal diseases in highly productive dairy cattle is acute. The current study presents the distribution of extracellular matrix in cows during physiological pregnancy ending in spontaneous labor (FB/SR) and complicated pregnancy with symptoms of preeclampsia/eclampsia ending in pathological  labor  (OB/PR)  using  histological, histochemical (HC) and immunohistochemical (IHC) research methods. Biopsy material of placentas was obtained from 10 cows during labor: from 5 cows with FB/SR and from 5  cows  with  OB/PR.  Samples  of  placentas from  cows  with  FB/SR  and  OB/PR  were subjected  to  standard  histological  procedures. Paraffin sections of the placenta, 5 µm thick, were mounted on glasses treated with poly-L-lysine (Menzel). Using IHC studies, the  immunolocalization  of  the  Ki-67  biomarker was studied. A histochemical study in  the  placentas  of  cows  with  FB/SR  revealed  the  presence  of  a  large  number  of small capillaries both in the villi of the cotyledons and in the caruncular crypts, as well as an equilibrium ratio of mesenchymal and epithelial  structures.  The  reaction  to  vimentin showed degradation of fibroblasts. In the  lumen  of  the  crypts,  only  structureless masses and a small number of macrophagelike cells were recorded. In addition, in the central part of the unchanged villi, a sharp decrease in the number of small capillaries was established, as well as a reduction in the terminal chorionic villi and a decrease in the vascularization of the remaining villi. Immunolocalization of the content of intermediate biomarkers  showed  the  content  of  a  large amount  of  Ki-67  in  the  cells  of  the  crypt lining. During pregnancy OB/PR, a decrease in the vascularization of chorionic villi in the cotyledons  and  degradation  of  cytokeratin filaments involved in the formation of cellular contacts between the cytotrophoblasts of the  chorionic villi  of  the  cotyledons of  the fetal part of the placenta and the epithelial cells  of  the  crypts  of  the  caruncles  of  the maternal  part  of  the  placenta  have  been demonstrated, which will make it possible in the future to develop an enzyme-linked immunosorbent assay (ELISA) as a rapid – test for  diagnosing  reproductive  pathologies  in dairy cattle. Pronounced expression of the Ki-67 protein was found in the cells lining the crypts of the caruncles, as well as in the cytoplasmic  membrane  of  the  villous  cytotrophoblasts. In the placenta of cows whose pregnancy  and  childbirth  were  complicated by pathology, the proliferative activity of the cells lining the uterine crypts remained at a physiological  level,  while  individual  chorionic  villi  were  completely  reduced,  and  in those  that  remained,  the  Ki-67  expression index was 36.94%.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"29 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological features of the wall of the small intestine of diurnal quails of meat breeds 肉用昼行性鹌鹑小肠壁的形态特征
International Journal of Veterinary Medicine Pub Date : 2023-12-16 DOI: 10.52419/issn2072-2419.2023.4.335
T. S. Golovchenko, V. V. Lemeshenko, T. P. Makalish
{"title":"Morphological features of the wall of the small intestine of diurnal quails of meat breeds","authors":"T. S. Golovchenko, V. V. Lemeshenko, T. P. Makalish","doi":"10.52419/issn2072-2419.2023.4.335","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.4.335","url":null,"abstract":"The  morphofunctional  status  of  the  digestive apparatus, including the small intestine, is decisive in the implementation of life-supporting functions both at the organ and at the organizational level, which is due not only to its participation directly in digestion, but  also  in  providing  immunogenesis  with tissue  components  of  the  intestinal  wall. However,  in  the  scientific  literature,  the structure  of  the  intestinal  wall  in  newborn quails  is  not  described  fully  enough,  there are no comparative data on the morphology of  the  small  intestine  within  the  group  of breeds belonging to the meat direction. The purpose of the study: to establish the features of the structure of the intestinal wall in diurnal quails of meat breeds. The breed features of the morphology of the small intestine of diurnal  quails  of  the  meat  breeds  Pharaoh, Texas  and  Japanese  by  n=4  were  studied using  a set of morphological techniques. It was found that the length and width of the villi prevail in the diurnal quails of the Japanese breed, as well as the number of goblet cells  in  the  duodenum  and  jejunum  compared with those of the Pharaoh and Texas quails,  while  the  smallest  values  of  the length and width of the villi, as well as the number of goblet cells, were revealed in the Pharaoh quails. The exception was the thickness of the serous membrane of the jejunum, which has the highest index in daily quails of the Pharaoh breed, and the lowest in quails of the Texas breed.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"65 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138967651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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