International Journal of Spectroscopy最新文献

Synthesis and Characterization of Cobalt(III) and Copper(II) Complexes of 2-((E)-(6-Fluorobenzo[d]thiazol-2-ylimino) methyl)-4-chlorophenol: DNA Binding and Nuclease Studies—SOD and Antimicrobial Activities 钴(III)和铜(II)配合物2-((E)-(6-氟苯并[d]噻唑-2-ylimino)甲基)-4-氯苯酚的合成和表征:DNA结合和核酸酶研究- sod和抗菌活性

International Journal of Spectroscopy Pub Date : 2018-03-01 DOI: 10.1155/2018/8759372

K. Savithri, B. C. V. Kumar, H. K. Vivek, H. D. Revanasiddappa

{"title":"Synthesis and Characterization of Cobalt(III) and Copper(II) Complexes of 2-((E)-(6-Fluorobenzo[d]thiazol-2-ylimino) methyl)-4-chlorophenol: DNA Binding and Nuclease Studies—SOD and Antimicrobial Activities","authors":"K. Savithri, B. C. V. Kumar, H. K. Vivek, H. D. Revanasiddappa","doi":"10.1155/2018/8759372","DOIUrl":"https://doi.org/10.1155/2018/8759372","url":null,"abstract":"A bidentate (N- and O-) imine-based ligand (L1) and its metal complexes of types [ (L1)2] (C1), [ (L1)(Phen)] (C2), [ (L1)2] (C3), and [ (L1)(Phen)] (C4) (L1 = 2-((E)-(6-fluorobenzo[d]thiazol-2-ylimino)methyl)-4-chlorophenol and phen = 1,10-phenanthroline) were synthesized as potential chemotherapeutic drug candidates. The prepared complexes were structurally characterized by spectral techniques (NMR, FT-IR, LC-MS, EPR, and electronic absorption), thermogravimetric analysis (TGA/DTA), magnetic moment, and CHNO elemental analysis. Spectroscopic studies suggested the distorted octahedral structure for all complexes. In vitro bioassay studies include binding and nuclease activities of the ligand and its complexes with target calf thymus- (CT-) DNA were carried out by employing UV-Vis, fluorescence spectroscopy, viscosity, and gel electrophoresis techniques. The extent of binding propensity was determined quantitatively by and values which revealed a higher binding affinity for C2 and C4 as compared to C1 and C3. In addition, the scavenging superoxide anion free radical ( ) activity of metal complexes was determined by nitroblue tetrazolium (NBT) light reduction assay. Molecular docking studies with DNA and SOD enzyme were also carried out on these compounds. The antimicrobial study has shown that all the compounds are potential antibacterial agents against Gram-negative bacterial strains and better antifungal agents with respect to standard drugs used.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"51 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90282041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Application of Electrochemical Techniques on Study of Effect of Nano-ZnO in Conductive Polyaniline Containing Zinc-Rich Primer 电化学技术在纳米zno导电聚苯胺富锌底漆中的应用研究

International Journal of Spectroscopy Pub Date : 2018-02-28 DOI: 10.1155/2018/7160381

Ximing Li, H. Castaneda

引用次数: 5

New Ni-Anthracene Complex for Selective and Sensitive Detection of 2,4,6-Trinitrophenol 新型ni -蒽配合物选择性灵敏检测2,4,6-三硝基苯酚

International Journal of Spectroscopy Pub Date : 2018-02-06 DOI: 10.1155/2018/1321427

Kumbam Lingeshwar Reddy, Anabathula Manoj Kumar, Abhimanew Dhir, V. Krishnan

引用次数: 5

Application of Autofluorescence for Analysis of Medicinal Plants 自体荧光技术在药用植物分析中的应用

International Journal of Spectroscopy Pub Date : 2017-11-26 DOI: 10.1155/2017/7159609

V. V. Roshchina, A. Kuchin, V. Yashin

引用次数: 17

Fourier Spectroscopy: A Bayesian Way 傅立叶光谱学:贝叶斯方法

International Journal of Spectroscopy Pub Date : 2017-10-31 DOI: 10.1155/2017/9265084

S. Schmuck, J. Svensson

{"title":"Fourier Spectroscopy: A Bayesian Way","authors":"S. Schmuck, J. Svensson","doi":"10.1155/2017/9265084","DOIUrl":"https://doi.org/10.1155/2017/9265084","url":null,"abstract":"The concepts of standard analysis techniques applied in the field of Fourier spectroscopy treat fundamental aspects insufficiently. For example, the spectra to be inferred are influenced by the noise contribution to the interferometric data, by nonprobed spatial domains which are linked to Fourier coefficients above a certain order, by the spectral limits which are in general not given by the Nyquist assumptions, and by additional parameters of the problem at hand like the zero-path difference. To consider these fundamentals, a probabilistic approach based on Bayes’ theorem is introduced which exploits multivariate normal distributions. For the example application, we model the spectra by the Gaussian process of a Brownian bridge stated by a prior covariance. The spectra themselves are represented by a number of parameters which map linearly to the data domain. The posterior for these linear parameters is analytically obtained, and the marginalisation over these parameters is trivial. This allows the straightforward investigation of the posterior for the involved nonlinear parameters, like the zero-path difference location and the spectral limits, and hyperparameters, like the scaling of the Gaussian process. With respect to the linear problem, this can be interpreted as an implementation of Ockham’s razor principle.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"16 1","pages":"1-29"},"PeriodicalIF":0.0,"publicationDate":"2017-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88752959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Annular Beam Shaping in Multiphoton Microscopy to Reduce Out-of-Focus Background 环形光束整形在多光子显微镜减少失焦背景

International Journal of Spectroscopy Pub Date : 2017-05-24 DOI: 10.1155/2017/7560141

Johan Borglin, Danni Wang, N. Durr, D. Hanstorp, A. Ben-Yakar, M. Ericson

{"title":"Annular Beam Shaping in Multiphoton Microscopy to Reduce Out-of-Focus Background","authors":"Johan Borglin, Danni Wang, N. Durr, D. Hanstorp, A. Ben-Yakar, M. Ericson","doi":"10.1155/2017/7560141","DOIUrl":"https://doi.org/10.1155/2017/7560141","url":null,"abstract":"Despite the inherent spatial confinement of multiphoton processes that arises from focusing through an objective, the maximum imaging depth in conventional multiphoton microscopy is ultimately limited by noise from out-of-focus fluorescence. This is particularly evident when imaging beyond shallow depths in highly scattering tissue as increased laser powers are necessary. The out-of-focus signal originates from multiphoton processes taking place primarily at shallow depths and deteriorates contrast and limits imaging depth. In this paper, annular laser beams are explored as a concept to reduce this background signal in multiphoton microscopy. The approach is theoretically verified by data from simulations and proof of principle is demonstrated on a custom-built experimental multiphoton microscopy platform. Annular laser beams were created by adopting wavefront control using a spatial light modulator and implemented for imaging tissue phantoms simulating turbid media and human skin ex vivo. The signal-to-background ratios were calculated and compared to images acquired with a traditional, filled-aperture Gaussian beam. Experiments in tissue phantom show an improvement in signal-to-background ratio of about 30% when using annular beam illumination in comparison to Gaussian illumination at specific depths. When laser power is not the limiting factor, this approach is expected to provide even greater benefits.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"45 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2017-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90492959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Nile Red and 2-NBDG Are Incompatible for the Simultaneous Detection of Lipid and Glucose Accumulation 尼罗红与2-NBDG在同时检测脂质和葡萄糖积累时不相容

International Journal of Spectroscopy Pub Date : 2016-12-22 DOI: 10.1155/2016/5215086

A. M. Hogan, Viswanathan Swaminathan, Nikitha K. Pallegar, S. L. Christian

{"title":"Nile Red and 2-NBDG Are Incompatible for the Simultaneous Detection of Lipid and Glucose Accumulation","authors":"A. M. Hogan, Viswanathan Swaminathan, Nikitha K. Pallegar, S. L. Christian","doi":"10.1155/2016/5215086","DOIUrl":"https://doi.org/10.1155/2016/5215086","url":null,"abstract":"Glucose is the universal energy source and a critical substrate for lipid synthesis in mammalian cells. Analysis of both glucose and lipid in cells is important for the understanding of the regulation of lipid synthesis in many cell types, but especially adipocytes, the major storage cell for fat in mammals. The fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD) derivative of glucose, 2-NBDG, is used to monitor glucose uptake and the lipid-selective fluorophore Nile red is used to monitor lipid accumulation. Previous reports have used NBD-based fluorophores and Nile red simultaneously despite the possibility of spectral overlap. In this study, we determined if these fluorophores were experimentally compatible in preadipocytes and adipocytes stained with 2-NBDG and Nile red separately or costained. We found that Nile red is detectable in the wavelengths necessary to excite and detect 2-NBDG. This interference was further increased by the solvatochromic effect of lipid-localized Nile red. In addition, we found a synergistic increase in fluorescent intensity when both fluorophores were present. Unfortunately, even fine control of the excitation or emission wavelengths did not identify wavelengths suitable for selective detection when cells were costained. Therefore, 2-NBDG and Nile red cannot be used simultaneously—but can likely be used sequentially—to assess glucose uptake and lipid accumulation in lipid-laden cells.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"140 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77522578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Quantum Chemical Calculations and Molecular Docking Studies of Some NSAID Drugs (Aceclofenac, Salicylic Acid, and Piroxicam) as 1PGE Inhibitors 一些非甾体抗炎药(醋氯芬酸、水杨酸和吡罗昔康)作为1PGE抑制剂的量子化学计算和分子对接研究

International Journal of Spectroscopy Pub Date : 2016-09-29 DOI: 10.1155/2016/5281636

S. Suresh, Sethu Gunasekaran, Shanmugam Srinivasan

{"title":"Quantum Chemical Calculations and Molecular Docking Studies of Some NSAID Drugs (Aceclofenac, Salicylic Acid, and Piroxicam) as 1PGE Inhibitors","authors":"S. Suresh, Sethu Gunasekaran, Shanmugam Srinivasan","doi":"10.1155/2016/5281636","DOIUrl":"https://doi.org/10.1155/2016/5281636","url":null,"abstract":"The molecular structure of the three compounds Aceclofenac (I), Salicylic Acid (II), and Piroxicam (III) has been determined using Gaussian 03W program with B3LYP method using 6-311++G (d,p) basis set calculations. The molecular structures were fully optimized with atomic numbering scheme adopted in the study. To understand the mode of binding and molecular interaction, the docking studies of compounds Aceclofenac (I), Salicylic Acid (II), and Piroxicam (III) have been carried out with prostaglandin H2 synthase-1 (1PGE) as target using induced fit docking. The molecular docking results show that the interactions and energy for Aceclofenac, Salicylic Acid, and Piroxicam show the best results when docked with prostaglandin H2 synthase-1 (1PGE). The hydrogen bonding interactions of compound I (Aceclofenac) are prominent with Arginine moiety, those of compound II (Salicylic Acid) are prominent with Tyrosine and Serine moieties, and compound III (Piroxicam) shows such interaction with Tyrosine and Arginine moieties. These interactions of prostaglandin H2 synthase-1 (1PGE) with substrates are responsible for governing COX-1 inhibitor potency which in turn is a direct measure of the potency of the drug.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"86 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83969715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Determination of Alcohol Content in Alcoholic Beverages Using 45 MHz Benchtop NMR Spectrometer 45兆赫台式核磁共振光谱仪测定酒精饮料中的酒精含量

International Journal of Spectroscopy Pub Date : 2016-08-17 DOI: 10.1155/2016/2526946

Meden F. Isaac-Lam

{"title":"Determination of Alcohol Content in Alcoholic Beverages Using 45 MHz Benchtop NMR Spectrometer","authors":"Meden F. Isaac-Lam","doi":"10.1155/2016/2526946","DOIUrl":"https://doi.org/10.1155/2016/2526946","url":null,"abstract":"Alcohol or ethanol is considered the most widely used recreational drug worldwide, and its production, consumption, and sale are strictly regulated by laws. Alcohol content of alcoholic beverages (wine, beers, and spirits) is about 3–50% v/v. Analytical methods to determine the alcohol content must be reliable, precise, and accurate. In this study, the amount of ethanol in several alcoholic beverages was determined using a 45 MHz low-field benchtop NMR (nuclear magnetic resonance) spectrometer. Internal standard and standard addition analytical methods were utilized to quantify ethanol. For both methods, acetic acid or acetonitrile was used as internal standard to quantify alcohol content by using the peak area corresponding to the methyl peaks of ethanol, acetic acid, or acetonitrile. Results showed that internal standard method gave values of percent alcohol that are in close agreement with the indicated label as confirmed by running the samples in a 400 MHz high-field NMR spectrometer using acetic acid as internal standard. This study demonstrates the utility of a benchtop NMR spectrometer that can provide an alternative technique to analyze percent alcohol in alcoholic products.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"17 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85321979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Atomic Structure Calculations for Neutral Oxygen 中性氧的原子结构计算

International Journal of Spectroscopy Pub Date : 2016-05-25 DOI: 10.1155/2016/1697561

N. Alonizan, R. Qindeel, N. B. Nessib

引用次数: 3