Korean Journal of Clinical Microbiology最新文献_第8页

Fungemia due to Exophiala dermatitidis 由外孢子性皮炎引起的真菌血症

Korean Journal of Clinical Microbiology Pub Date : 2010-09-01 DOI: 10.5145/KJCM.2010.13.3.135

Eun-Sun Jeong, Jong‐Hee Shin, M. Shin, S. Suh, D. Ryang

引用次数: 3

Aberrant Forms of Escherichia coli in Urine Culture 尿培养中大肠杆菌的异常形式

Korean Journal of Clinical Microbiology Pub Date : 2010-09-01 DOI: 10.5145/KJCM.2010.13.3.128

Youngeun Ma, J. Lee, Seung-Tae Lee, C. Ki, N. Lee

引用次数: 0

Importance of Suspicion for the Identification of Mycoplasma in Wound Culture: A Case Report 怀疑对伤口培养支原体鉴定的重要性:1例报告

Korean Journal of Clinical Microbiology Pub Date : 2010-09-01 DOI: 10.5145/KJCM.2010.13.3.121

S. M. Hwang, I. Yoon, S. Joo, Jongyoun Yi, E. Kim

引用次数: 0

Registration of KJCM on the Journal List of 'National Research Foundation' 《中国科学院学报》在“国家研究基金”期刊名单上的登记

Korean Journal of Clinical Microbiology Pub Date : 2010-09-01 DOI: 10.5145/KJCM.2010.13.3.144

Sunjoo Kim

引用次数: 0

Detection of Enterovirus using Real-Time Nucleic Acid Sequence-based Amplification. 基于实时核酸序列扩增技术检测肠道病毒。

Korean Journal of Clinical Microbiology Pub Date : 2010-06-01 DOI: 10.5145/KJCM.2010.13.2.53

S. Jun, Kee-Hyung Sung, S. Song, K. Park, Hong-Bin Kim, Junghan Song, E. Choi, S. Park, E. Kim

{"title":"Detection of Enterovirus using Real-Time Nucleic Acid Sequence-based Amplification.","authors":"S. Jun, Kee-Hyung Sung, S. Song, K. Park, Hong-Bin Kim, Junghan Song, E. Choi, S. Park, E. Kim","doi":"10.5145/KJCM.2010.13.2.53","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.53","url":null,"abstract":"Background: Enteroviruses are the most frequent etiologic agents of aseptic meningitis and are estimated to be the cause of 70% to 90% of viral meningitis cases. Enterovirus diagnosis can be difficult because clinical features vary according to patient immunity and age. The purpose of this study was to evaluate the performance of the real-time nucleic acid sequence-based amplification (NASBA) assay compared to that of the real-time nested RT-PCR assay for enterovirus detection. Methods: This study was performed on 96 patients suspected of aseptic meningitis based on clinical features. RNA was extracted using NucliSENS EasyMAG and real-time NASBA assay was performed using NucliSENS EasyQ Enterovirus and NucliSENS EasyQ Basic 2. We also executed in-house real-time nested RT-PCR assay for RNA extracted via QIAamp Viral RNA Mini. Results: The positive rate of real-time NASBA assay was 45.8% for enterovirus detection. The positive rate of first real-time reverse transcription PCR was 22.9% and the second real-time PCR was 57.3%. The concordant rate of the real-time NASBA assay and first real-time reverse transcription PCR was 75.0%. The concordant rate of the real-time NASBA assay and second real-time PCR was 86.5%. Conclusion: The detection of enteroviruses using the real-time NASBA assay is less prone to cross-contamination and is simple, without the need for reverse transcription. We conclude that the NASBA assay is an effective method for the rapid diagnosis of aseptic meningitis. (Korean J Clin Microbiol 2010;13: 53-58)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127197238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Revised Penicillin Breakpoints for Streptococcus pneumoniae (CLSI M100-S18) on the Penicillin Susceptibility Rate 修订肺炎链球菌青霉素断点(CLSI M100-S18)对青霉素敏感性的影响

Korean Journal of Clinical Microbiology Pub Date : 2010-06-01 DOI: 10.5145/KJCM.2010.13.2.68

Kyung-Hee Kim, Jung-eun Kim, S. Park, Y. Song, J. Ahn, P. Park, Y. Seo

{"title":"Impact of Revised Penicillin Breakpoints for Streptococcus pneumoniae (CLSI M100-S18) on the Penicillin Susceptibility Rate","authors":"Kyung-Hee Kim, Jung-eun Kim, S. Park, Y. Song, J. Ahn, P. Park, Y. Seo","doi":"10.5145/KJCM.2010.13.2.68","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.68","url":null,"abstract":"Background: In January 2008, the Clinical and Laboratory Standards Institute (CLSI) published revised penicillin breakpoints for Streptococcus pneumoniae according to clinical presentation and the route of penicillin administration. The aim of this study was to evaluate the impacts of the new penicillin breakpoints on the susceptibility rates of S. pneumoniae isolated from blood. Methods: A total of 156 non-duplicated S. pneumoniae strains recovered from blood of hospitalized patients were collected between January 2003 and December 2008. Penicillin and cefotaxime susceptibility tests were performed using an E-test (AB Biodisk, Solna, Sweden). Results of the penicillin susceptibility tests were analyzed using the former and new CLSI guidelines. Results: Of the 156 S. pneumoniae strains isolated from blood, penicillin susceptibility under the former CLSI guidelines resulted in 42.3% susceptible, 42.3% intermediate, and 15.4% resistant states. According to the new CLSI guidelines (nonmeningitis, parenteral), 87.8% of isolates were susceptible, 9.6% were intermediate, and 2.6% were resistant to penicillin. Conclusion: When the new CLSI guidelines are applied, the penicillin susceptibility rate of S. pneumoniae strains isolated from blood is considerably increased. This suggests that penicillin should still be useful for the treatment of nonmeningeal pneumococcal infections and that the use of broad-spectrum antimicrobials should not replace this treatment. (Korean J Clin Microbiol 2010;13:68-72)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"200 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114876109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

A Case of Verotoxin-producing Escherichia coli O157:H7 with Hemorrhagic Colitis in an Infant, Diagnosed by Multiplex PCR 产维罗毒素大肠杆菌O157:H7合并出血性结肠炎1例，多重PCR诊断

Korean Journal of Clinical Microbiology Pub Date : 2010-06-01 DOI: 10.5145/KJCM.2010.13.2.85

Haewol Cho, M. Cho, Shin-Ae Noh, M. N. Kim, Kyoung Mo Kim

{"title":"A Case of Verotoxin-producing Escherichia coli O157:H7 with Hemorrhagic Colitis in an Infant, Diagnosed by Multiplex PCR","authors":"Haewol Cho, M. Cho, Shin-Ae Noh, M. N. Kim, Kyoung Mo Kim","doi":"10.5145/KJCM.2010.13.2.85","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.85","url":null,"abstract":"Enterohemorrhagic Escherichia coli (EHEC) is an important cause of bloody diarrhea in children, but is considered to be rare in infants. Herein, a case of infant hemorrhagic colitis of verotoxin-producing E. coli O157:H7 diagnosed by multiplex PCR is reported. A nine-month-old boy was admitted to our hospital with bloody diarrhea for the previous two days. Multiplex PCR using SeeplexR Diarrhea ACE Detection Kit (Seegene, Seoul, Korea) was directly applied to the stool specimens. Amplified bands specific for verotoxin, O157, and H7 indicated the presence of O157:H7 EHEC. The stool specimens were inoculated on sorbitol-MacConkey agar (SMA) and tryptic soy broth containing mitomycin C (TSB-M). Colorless colonies on sorbitol-MacConkey agar were O157-positive. TSB-M enrichment cultures of the stool specimen and the isolates were positive for verotoxin according to an enzyme immunoassay (EIA). The prepared ingredients of baby foods for the patient including ground meat, chopped carrot, chopped cabbage, and white rice porridge showed no EHEC on TSB-M and SMA. The patient’s parents and three-year-old sister did not recently have any gastrointestinal symptoms. Cefdinir was administered for one day and was ceased after diagnosis of EHEC colitis. The stool culture and verotoxin assay were negative on the second day of hospitalization. Application of multiplex PCR and verotoxin EIA directly to diarrheal stool warrants the rapid diagnosis and appropriate treatment of EHEC colitis. (Korean J Clin Microbiol 2010;13:85-89)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131266495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A Case of Allergic Fungal Sinusitis due to Curvularia 曲曲霉致变应性真菌鼻窦炎1例

Korean Journal of Clinical Microbiology Pub Date : 2010-06-01 DOI: 10.5145/KJCM.2010.13.2.98

Hae-Sun Chung, J. Lee, H. Kim, N. Lee

引用次数: 0

A Case Report of Tsukamurella pulmonis Infection Misidentified as Atypical Mycobacteria 肺恙螨感染误诊为非典型分枝杆菌1例

Korean Journal of Clinical Microbiology Pub Date : 2010-06-01 DOI: 10.5145/KJCM.2010.13.2.93

A. R. Cho, H. Kim, Mi-Kyung Lee, Seong-Ho Choi, S. Yun

引用次数: 4

Clostridium symbiosum Isolated from Blood 从血液中分离的共生梭菌

Korean Journal of Clinical Microbiology Pub Date : 2010-06-01 DOI: 10.5145/KJCM.2010.13.2.90

H. Huh, Seung-Tae Lee, J. Lee, C. Ki, N. Lee

引用次数: 1