Korean Journal of Clinical Microbiology最新文献_第6页

Comparison of Clinical and Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing Breakpoints for beta-Lactams in Enterobacteriaceae Producing Extended-Spectrum beta-Lactamases and/or Plasmid-Mediated AmpC beta-Lacta 临床和实验室标准协会和欧洲抗菌药物敏感性测试委员会在产生广谱β -内酰胺酶和/或质粒介导的AmpC β -内酰胺的肠杆菌科细菌中β -内酰胺的断点的比较

Korean Journal of Clinical Microbiology Pub Date : 2011-03-01 DOI: 10.5145/KJCM.2011.14.1.24

W. Song, Min-Jeong Park, H. Kim, Jae Seok Kim, H. S. Kim, K. Lee

引用次数: 2

Evaluation of Automated Blood Culture System for Body Fluids Culture Other Than Blood 自动血液培养系统对非血液体液培养的评价

Korean Journal of Clinical Microbiology Pub Date : 2011-03-01 DOI: 10.5145/KJCM.2011.14.1.13

T. Choi, J. Kang, H. Pai

{"title":"Evaluation of Automated Blood Culture System for Body Fluids Culture Other Than Blood","authors":"T. Choi, J. Kang, H. Pai","doi":"10.5145/KJCM.2011.14.1.13","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.1.13","url":null,"abstract":"Background: We investigated whether culture using an automated blood culture system enhances the recovery of bacteria and fungi from body fluids other than blood when compared to conventional solid media culture methods. Methods: A total of 734 specimens [ascites (n=457), bile (n=5), CAPD (n=28), CSF (n=32), joint fluids (n= 165), pericardial fluid (n=17), and pleural fluid (n=30)] were included in the study. Half of the volume of each specimen was inoculated directly into automated blood culture bottles (bioMeriux, Marcy-I’Etoile, France). The remaining volume was inoculated onto conventional solid media (sheep blood agar, chocolate agar, and phenylethyl alcohol agar) after centrifuging at 3,000 rpm for 10 min. Results: Clinically significant microorganisms were isolated from 62 specimens (8.5%) by automated blood culture and 61 specimens (8.3%) by the conventional solid media culture (kappa index: 0.81, 95% confidence interval: 0.75∼0.89). Contamination was observed in 11 (1.8%) of the automated blood culture specimens and 3 (0.4%) of the solid media culture specimens. The mean turnaround times of the automated blood cultures and the conventional solid media cultures were 3.7 and 2.8 days, respectively (P< 0.0001). Conclusion: Compared with conventional culture methods, no improvement in the recovery of clinically significant microorganisms was noted with the use of the automated blood culture system for the culture of body fluids other than blood. (Korean J Clin Microbiol 2011;14:13-17)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"243 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134085046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Case of a 63-bp Deletion in the mpt64 Gene of Mycobacterium tuberculosis Strains Which Showed False Negativity in the Immunochromatographic Assay 结核分枝杆菌mpt64基因63-bp缺失免疫层析检测假阴性1例

Korean Journal of Clinical Microbiology Pub Date : 2011-03-01 DOI: 10.5145/KJCM.2011.14.1.36

C. Yoon, Y. Hong, Jin Kyung Lee, Yoon Hwan Chang, Seokil Hong

引用次数: 0

Attending the 22nd Annual Meeting of Japanese Society for Clinical Microbiology 参加第22届日本临床微生物学会年会

Korean Journal of Clinical Microbiology Pub Date : 2011-03-01 DOI: 10.5145/KJCM.2011.14.1.39

Sunjoo Kim

引用次数: 0

Clinical Implications of Multiplex PCR Detection of Fastidious Microorganisms in Vaginitis Patients. 多重PCR检测阴道炎患者挑剔微生物的临床意义。

Korean Journal of Clinical Microbiology Pub Date : 2011-03-01 DOI: 10.5145/KJCM.2011.14.1.30

Nae Yu, Mi-Kyung Lee

{"title":"Clinical Implications of Multiplex PCR Detection of Fastidious Microorganisms in Vaginitis Patients.","authors":"Nae Yu, Mi-Kyung Lee","doi":"10.5145/KJCM.2011.14.1.30","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.1.30","url":null,"abstract":"Background: Bacterial vaginitis (BV) and Trichomonas vaginitis are the most frequently recurring infectious diseases in women. Therefore, accurate tests for post-treatment follow-up are required. A multiplex PCR assay allows for the simultaneous detection of multiple pathogens in a single specimen. In this study, we assessed the clinical implications of multiplex PCR detection of fastidious microorganisms causing vaginitis. Methods: A total of 216 vaginitis patients who presented to Chung-Ang University Yongsan Hospital with more than one positive result on multiplex PCR (Trichomonas vaginalis (TV), Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), Mycoplasma genitalium (MG), Mycoplasma hominis (MH)) were retrospectively enrolled in this study. Each patient’s clinical symptoms, initial treatment and follow-up for BV, and other related test results were also retrospectively reviewed. Results: The most commonly reported symptom was abnormal discharge, followed by pruritis (73.1%), lower abdominal pain (38.4%), urination difficulties (13%), and others such as fever. According to the multiplex PCR results, there were 116 cases (35.8%) of MH, 86 cases (26.5%) of UU, 62 cases (19.1%) of CT, and 84 cases (38.9%) were mixed infections. Among those patients with single infections, treatment changed for 63 cases (65.6%) while treatment remained unchanged for 17 (17.7%) after PCR results were reported. Conclusion: The diagnosis of BV using multiplex PCR is clinically effective and the results of which can be incorporated in antibiotic selection for patients with multiple sexually transmitted diseases (STD). Multiplex PCR may be especially helpful in the diagnosis of patients in whom the differentiation of STD pathogens is difficult using traditional methods. (Korean J Clin Microbiol 2011;14:30-35)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133808994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Prevalence and Clinical Characteristics of Mupirocin-Resistant Staphylococcus aureus 耐多匹罗星金黄色葡萄球菌的流行及临床特点

Korean Journal of Clinical Microbiology Pub Date : 2011-03-01 DOI: 10.5145/KJCM.2011.14.1.18

A. J. Lee, H. Suh, C. Jeon, Sang Gyung Kim

{"title":"Prevalence and Clinical Characteristics of Mupirocin-Resistant Staphylococcus aureus","authors":"A. J. Lee, H. Suh, C. Jeon, Sang Gyung Kim","doi":"10.5145/KJCM.2011.14.1.18","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.1.18","url":null,"abstract":"Background: Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) is a known risk factor for nosocomialtransmission and infection. In an effort to mitigate this problem, topical mupirocin has been widely used for clearing nasal carriage of MRSA. However, mupirocin resistance has become a worldwide concern due to increased use of the antibiotic. The aims of this study were to evaluate the clinical characteristics and prevalence of mupirocin resistance among clinical isolates of staphylococci and to investigate antimicrobial susceptibility. Methods: A total of 175 S. aureus specimens recovered over a 4-month period from various body sites were tested for resistance to mupirocin and other antibiotics using the Vitek2 automated system. The presence of the mupA gene was assessed in isolates exhibiting resistance to mupirocin and in other selected organisms. The clinical characteristics of the isolates were also reviewed. Results: Of the 175 S. aureus isolates, 9.1% (16/175) were resistant to mupirocin, with 1.7% (3/175) having high-level resistance (HR) and 7.4% (13/175) having low-level resistance (LR). Patients with HR-mupirocinresistant S. aureus had a longer duration of hospitalization (P=0.026). Of the 13 LR-mupirocin-resistant S. aureus strains, 11 had identical antibiogram patterns. The mupA gene was detected only among HR isolates. Conclusion: The rate of mupirocin resistance in the S. aureus isolates was high. The spread of mupirocinresistant S. aureus may be due to nosocomial infection. (Korean J Clin Microbiol 2011;14:18-23)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129606432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Korean Journal of Clinical Microbiology Pub Date : 2010-12-01 DOI: 10.5145/KJCM.2010.13.4.173

H. Woo, J. Lee, Seung-Tae Lee, C. Ki, N. Lee

引用次数: 2

Comparison of SD BIOLINE Rapid Influenza Antigen Test Using Two Different Specimens, Nasopharyngeal Swabs and Nasopharyngeal Aspirates SD BIOLINE快速流感抗原检测中鼻咽拭子和鼻咽吸入液两种不同标本的比较

Korean Journal of Clinical Microbiology Pub Date : 2010-12-01 DOI: 10.5145/KJCM.2010.13.4.147

H. I. Bang, J. Shin, T. Choi, R. Park, Yu Jeong Shin

{"title":"Comparison of SD BIOLINE Rapid Influenza Antigen Test Using Two Different Specimens, Nasopharyngeal Swabs and Nasopharyngeal Aspirates","authors":"H. I. Bang, J. Shin, T. Choi, R. Park, Yu Jeong Shin","doi":"10.5145/KJCM.2010.13.4.147","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.4.147","url":null,"abstract":"Background: The pandemic swine origin influenza A/H1N1 2009 virus (H1N1 2009) was rapidly spread out all over the world after it was first found in April, 2009. This study was made to compare the performance of nasopharyngeal swabs and nasopharyngeal aspirates for the SD Bioline rapid influenza antigen test. Methods: From Aug to Nov, 2009 the SD Bioline rapid influenza antigen tests were conducted with the nasopharyngeal swabs and the nasopharyngeal aspirates from the 244 specimens of patients who had come to the hospital with influenza-like illness. The data from the examination were compared with the multiplex RT-PCR as a reference standard to obtain sensitivity, specificity, positive predictive value and negative predictive value. Results: The sensitivity and the specificity of the SD Bioline rapid influenza antigen tests with the nasopharyngeal swabs were 75.8%, and 93.3% respectively, and the sensitivity and specificity with the nasopharyngeal aspirates were 61.3%, and 98.3% respectively. Conclusion: Even if the nasopharyngeal aspirates showed the lower sensitivity than the nasopharyngeal swabs, since the specificity is higher, the nasopharyngeal aspirates are more useful because we can reduce false positive rate. (Korean J Clin Microbiol 2010;13:147-150)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123774092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparison of BACTEC Plus Aerobic/F Media and BacT/ Alert FA Media to Detect Bacteria in Blood Culture Bottles Containing Peak Therapeutic Levels of Antimicrobials BACTEC +有氧/F培养基与BACTEC / Alert FA培养基检测抗微生物药物峰值治疗水平血培养瓶细菌的比较

Korean Journal of Clinical Microbiology Pub Date : 2010-12-01 DOI: 10.5145/KJCM.2010.13.4.151

Jin Young Lee, J. Hong, Miae Lee

{"title":"Comparison of BACTEC Plus Aerobic/F Media and BacT/ Alert FA Media to Detect Bacteria in Blood Culture Bottles Containing Peak Therapeutic Levels of Antimicrobials","authors":"Jin Young Lee, J. Hong, Miae Lee","doi":"10.5145/KJCM.2010.13.4.151","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.4.151","url":null,"abstract":"Background: Blood culture bottles with an antimicrobial removal system have been developed for patients treated with antibiotics. This study compared the ability of BACTEC Plus Aerobic/F bottles (Becton Dickinson, USA, BACTEC Plus) and BacT/Alert FA bottles (bioMerieux Vitek, France) to effectively remove antimicrobials. Methods: BACTEC Plus and BacT/Alert FA bottles were spiked with 5 mL human blood, peak therapeutic concentrations of 9 antimicrobials and 7 type strains. Three rounds of duplicate testing were completed per antimicrobial/strain combination and growth control without antimicrobials. The time to detection (TTD) and recovery rates for bacteria were compared for both systems. Results: Overall, the BACTEC Plus and BacT/Alert FA recovered 76% (128/168) and 34% (57/168) of strains from test bottles, respectively. BACTEC Plus detected all of gram-positive bacteria except S. pneumoniae with ampicillin and ceftriaxone, but BacT/Alert FA detected 0∼50% of gram-positive bacteria except E. faecalis with vancomycin and methicillin-resistant S. aureus with oxacillin. In presence of cefepime, cefotaxime, cefoxitin and ceftriaxone, BACTEC Plus detected 33∼100% of gram-negative bacteria, but BacT/ Alert FA did not detect gram-negative bacteria at all. In presence of ciprofloxacin, BacT/Alert FA detected 100% of E. coli and K. pneumoniae compared with 33% of those for BACTEC Plus. Overall, TTD of BACTEC Plus was shorter than that of BacT/Alert FA except in detecting gram-negative bacteria with ciprofloxacin (P＜0.05). Conclusion: BACTEC Plus Aerobic/F media containing peak therapeutic levels of antimicrobials are more effective and faster detection of bacteria than BacT/ Alert FA media. (Korean J Clin Microbiol 2010;13: 151-156)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122203322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Prevalence of Metallo-β-lactamases in Imipenem-non-susceptible Pseudomonas aeruginosa and Acinetobacter baumannii 金属β-内酰胺酶在亚胺培南非敏感铜绿假单胞菌和鲍曼不动杆菌中的流行

Korean Journal of Clinical Microbiology Pub Date : 2010-12-01 DOI: 10.5145/KJCM.2010.13.4.169

N. Ryoo, J. Ha, D. Jeon, Jae Ryong Kim

{"title":"Prevalence of Metallo-β-lactamases in Imipenem-non-susceptible Pseudomonas aeruginosa and Acinetobacter baumannii","authors":"N. Ryoo, J. Ha, D. Jeon, Jae Ryong Kim","doi":"10.5145/KJCM.2010.13.4.169","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.4.169","url":null,"abstract":"Background: Metallo-β-lactamases (MBLs) have been reported in gram negative bacilli and are becoming increasingly important clinically because the enzymes hydrolyse almost all β-lactams, including carbapenems. Thus, the present study was conducted to determine the prevalence of MBL types in imipenem-nonsusceptible Pseudomonas aeruginosa and Acinetobacter baumannii isolated from a tertiary teaching hospital. Methods: Imipenem-nonsusceptible strains, 128 P. aeruginosa and 93 A. baumannii, were collected from clinical specimens. Identification and susceptibility tests were determined by Vitek GNI and GNS cards. MBL production was determined by modified Hodge test and imipenem-EDTA synergy test. Multiplex PCR amplification of MBL genes including blaIMP-1, blaVIM-1 and blaVIM-2 were performed. Results: Thirty-one P. aeruginosa (24.2%) isolates and 3 A. baumannii (3.2%) were found to be MBL producers. In P. aeruginosa, 20 (15.6%) and 11 (8.6%) isolates were positive for blaIMP-1 and blaVIM-2, respectively whereas 1 (1.0%) and 2 (2.2%) isolates in A. baumannii, respectively. Conclusion: IMP-1 is more prevalent MBL type than VIM-2 among imipenem-nonsusceptible P. aeruginosa unlike in other studies. Larger numbers of isolates and sequential studies are strongly recommended for the useful evaluation and monitoring of MBL production in the hospital setting to infection-control. (Korean J Clin Microbiol 2010;13:169-172)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132854961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12