Korean Journal of Clinical Microbiology最新文献_第4页

Three Adult Cases of Elizabethkingia meningoseptica Infection in a Korean Hospital 韩国医院成人伊莉莎白菌脑膜炎败血症感染3例

Korean Journal of Clinical Microbiology Pub Date : 2011-12-01 DOI: 10.5145/KJCM.2011.14.4.153

Jayoung Kim, Y. Park, Soyoung Shin, Yonggoo Kim, S. Park

引用次数: 1

Evaluation of a Newly Developed Multiplex Real-time PCR Assay for the Detection of Vancomycin-Resistant Enterococci from Rectal Swabs 直肠拭子中万古霉素耐药肠球菌多重实时荧光定量PCR检测方法的评价

Korean Journal of Clinical Microbiology Pub Date : 2011-12-01 DOI: 10.5145/KJCM.2011.14.4.138

M. Jung, W. Lee, Myungsun Park

{"title":"Evaluation of a Newly Developed Multiplex Real-time PCR Assay for the Detection of Vancomycin-Resistant Enterococci from Rectal Swabs","authors":"M. Jung, W. Lee, Myungsun Park","doi":"10.5145/KJCM.2011.14.4.138","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.4.138","url":null,"abstract":"Background: Asymptomatic vancomycin-resistant enterococci (VRE) colonization precedes infection. VREcolonized patients serve as silent reservoirs of enterococci that go on to colonize other patients. Rapidly identifying colonized patients is crucial to prevent the spread of VRE. The culture-based method of VRE screening is time-consuming. We evaluated the diagnostic performance of a recently developed multiplex real-time PCR for the detection of VRE. Methods: We obtained 105 rectal swabs from patients who were being monitored for carriage of VRE. After 24 hour incubation of swabs in enterococcosel broth (EB) supplemented with 6 μg/mL vancomycin, multiplex real-time PCR was performed using the Anyplex VanR Real-time Detection (VanR) kit (Seegene, Inc., Seoul, Korea). The results of multiplex real-time PCR were compared to those of culture. We evaluated the specificity and detection limits of multiplex real-time PCR using VanR for VRE. Results: A total of 96/105 (91.4%) samples were VRE positive according to multiplex real-time PCR with EB while 85/105 (80.9%) samples were positive in culture. Eleven discordant results (10.4%) (multiplex real-time PCR positive, culture negative) were noted. All non-enterococcal bacteria and vancomycin-susceptible enterococci were negative. The DNA detection limits of VanR were 0.035 pg per reaction (3 μL) for Enterococcus faecium and 0.35 pg for Enterococcus faecalis. Conclusion: The application of multiplex real-time PCR after EB incubation allows rapid and sensitive detection in 26-28 hours for VRE screening from rectal swabs. This method could facilitate the timely implementation of contact isolation to prevent the spread of VRE. (Korean J Clin Microbiol 2011;14: 138-143)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129028541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Cases of Cryptococcuria Developed as Isolated Cryptococcuria and Disseminated Cryptococcosis. 隐球菌病发展为分离性隐球菌病和播散性隐球菌病2例。

Korean Journal of Clinical Microbiology Pub Date : 2011-12-01 DOI: 10.5145/KJCM.2011.14.4.148

M. Bae, S. Namgoong, Dongheui An, M. N. Kim, Sung‐Han Kim, K. Park, Sung Gyu Lee

{"title":"Two Cases of Cryptococcuria Developed as Isolated Cryptococcuria and Disseminated Cryptococcosis.","authors":"M. Bae, S. Namgoong, Dongheui An, M. N. Kim, Sung‐Han Kim, K. Park, Sung Gyu Lee","doi":"10.5145/KJCM.2011.14.4.148","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.4.148","url":null,"abstract":"Cryptococcus is an opportunistic pathogen that mainly affects immunocompromised hosts and, less frequently, immunocompetent hosts. It causes serious morbidity and mortality due to systemic infections such as meningoencephalitis and pulmonary infection. Urinary involvement of Cryptococcus is sometimes reported among cases of disseminated cryptococcosis in AIDS patients, but no such reports have been published in Korea. We report two cases of cryptococcuria that developed in a 71-year old female with diabetes and liver cirrhosis and in a 50-year old male who received a liver transplant due to HBV-associated hepatic failure. The female patient had received prednisolone for 12 days before we detected C. neoformans in urine culture. Even though no antifungal therapy was indicated for cryptococcuria, following urine culture became negative, but still positive for cryptococcal antigen on hospital day 25. Her blood, CSF culture, and antigen tests were negative, and therefore she was diagnosed with isolated cryptococcuria. The male patient had received prednisolone and tacrolimus for 10 days before sputum and urine cultures became positive for C. neoformans. He had ill defined nodules and pleural effusion in both lungs on chest CT. His cryptococcuria was sustained for over 2 months, despite receiving amphotericin B treatment. His cryptococcuria seemed to be a symptom of disseminated cryptococcosis. (Korean J Clin Microbiol 2011;14:148-152)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132081949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Epidemiology and Erythromycin Resistance of Streptococcus pyogenes in the Last 20 Years 近20年化脓性链球菌流行病学及红霉素耐药性分析

Korean Journal of Clinical Microbiology Pub Date : 2011-12-01 DOI: 10.5145/KJCM.2011.14.4.119

Sunjoo Kim

{"title":"Epidemiology and Erythromycin Resistance of Streptococcus pyogenes in the Last 20 Years","authors":"Sunjoo Kim","doi":"10.5145/KJCM.2011.14.4.119","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.4.119","url":null,"abstract":"Serological methods for the epidemiological study of Streptococcus pyogenes, such as T-, or M-typing, were replaced by emm typing in the 2000s. The frequency of emm types may differ according to geographical area and study period. Erythromycin resistance rates and the prevalence of erythromycin-resistant phenotypes in several countries are surveyed, and common emm genotypes associated with erythromycin resistance are described for each country. There is no correlation between erythromycin resistance and macrolide use in Korea. S. pyogenes is thought to cause severe illness, such as streptococcal toxic shock syndrome (STSS) and necrotizing fasciitis. The mortality rate of STSS is about 50%, and there have been several hundred victims of STSS in Japan in the last 2 decades. The resistance rate to macrolides peaked at 50% in 2002, and currently, ＜10% of strains exhibit macrolide resistance in Korea. However, the erythromycin resistance rate recently exceeded 90% in China. Considering increases of travel between neighboring countries, a vigilant survey to monitor these highly virulent and antibiotic-resistant strains is necessary. (Korean J Clin Microbiol 2011;14:119-125)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129291042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of MolecuTech Real MTB-ID for MTB/NTM Detection Using Direct Specimens 直接标本检测MTB/NTM的MolecuTech Real MTB- id评价

Korean Journal of Clinical Microbiology Pub Date : 2011-09-01 DOI: 10.5145/KJCM.2011.14.3.103

Hye-young Wang, Hyunwoo Jin, H. Bang, Yeon Im Choi, E. Park, W. Koh, Hyeyoung Lee

引用次数: 2

Removal of PCR Inhibitors in Real-time PCR for Mycobacterium tuberculosis 结核分枝杆菌实时荧光定量PCR中PCR抑制剂的去除

Korean Journal of Clinical Microbiology Pub Date : 2011-09-01 DOI: 10.5145/KJCM.2011.14.3.97

H. Yun, H. Kim, Young Kyung Lee, H. Kang, Jae Seok Kim, W. Song, K. Lee

{"title":"Removal of PCR Inhibitors in Real-time PCR for Mycobacterium tuberculosis","authors":"H. Yun, H. Kim, Young Kyung Lee, H. Kang, Jae Seok Kim, W. Song, K. Lee","doi":"10.5145/KJCM.2011.14.3.97","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.3.97","url":null,"abstract":"Background: The inhibition rates for nucleic acid tests of Mycobacterium tuberculosis have been reported to range from less than 1% to more than 10%. Specimen dilution, boiling, addition of bovine serum albumin (BSA), and a silica membrane can be used to override amplification inhibitors in nucleic acid tests of M. tuberculosis. The inhibition rate for real-time PCR of M. tuberculosis (COBAS TaqMan MTB test; Roche Diagnostics, Manheim, Germany) and effective strategies to override PCR inhibitors were investigated in this study. Methods: The inhibition rate for COBAS TaqMan MTB test was investigated in 980 clinical specimens. The effectiveness of PCR inhibitor removal by repeated run, dilution, boiling, addition of BSA, and use of silica membrane were evaluated in the inhibited specimens. Results: Inhibitory substances were present in 4.1% of specimens (40/980). Among 40 inhibited specimens, inhibitory substances were removed in 12 (30%), 30 (75%), 27 (67.5%), 25 (62.5%) and 12 (30%) specimens with repeated run, dilution, addition of RBS, boiling and use of silica membrane, respectively. Conclusion: The overall inhibition rate for the COBAS TaqMan MTB test was 4.1%. Dilution, boiling and addition of BSA were shown to be more effective than repeated run and use of silica membrane for removal of PCR inhibitors. A combination of two methods might be useful and should be studied in the future. (Korean J Clin Microbiol 2011;14:97-102)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128545754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudo-outbreak of Brevundimonas diminuta 假的小芽胞杆菌爆发

Korean Journal of Clinical Microbiology Pub Date : 2011-09-01 DOI: 10.5145/KJCM.2011.14.3.115

Y. Kim, E. Koh, Yong Wha Lee, You Kyoung Lee, H. Shin, Kyungwon Lee

引用次数: 2

Campylobacter jejuni Bacteremia in a Healthy Child 健康儿童空肠弯曲杆菌菌血症

Korean Journal of Clinical Microbiology Pub Date : 2011-09-01 DOI: 10.5145/KJCM.2011.14.3.110

Min Jin Kim, S. Y. Kim, Y. Park, H. Yoon, J. Suh, Hee-Joo Lee

引用次数: 1

Nationwide Survey of Blood Culture Performance Regarding Skin Disinfection, Blood Collection and Laboratory Procedures 关于皮肤消毒、采血和实验室程序的全国血液培养效果调查

Korean Journal of Clinical Microbiology Pub Date : 2011-09-01 DOI: 10.5145/KJCM.2011.14.3.91

J. Shin, S. Song, M. N. Kim, Sunjoo Kim

{"title":"Nationwide Survey of Blood Culture Performance Regarding Skin Disinfection, Blood Collection and Laboratory Procedures","authors":"J. Shin, S. Song, M. N. Kim, Sunjoo Kim","doi":"10.5145/KJCM.2011.14.3.91","DOIUrl":"https://doi.org/10.5145/KJCM.2011.14.3.91","url":null,"abstract":"Background: Although many laboratories use automated blood culture systems, adequate skin disinfection and optimal blood volume are still critical for successful culture. The authors undertook a nationwide survey to understand the current situation and problems of blood culture in Korea. Methods: A survey of blood culture was performed in March and April 2010, including disinfectants, blood collection intervals, and recommended blood volumes. The laboratory physicians described the storage condition of culture bottles before delivery to the equipment. For quality control, the positive rate and skin contamination rate were studied. Results: Replies to the survey were collected from 74 Korean hospitals. Povidone iodine after either isopropyl alcohol or ethanol application was the most common means of skin disinfection. Sampling of a second set of cultures was performed simultaneously in 38% of hospitals and after a 30-min interval in 50%. The recommended blood volume was 10 mL in most cases (69%), but was 20 mL in 24% of cases. The bottles were stored at 37C before installation in 23% of cases and at room temperature in 16%, whereas 57% were placed directly in the equipment during the night shift. Positive rates ranged 8-10% in 32% of hospitals, 5-8% in 23%, and 3% in 13%. Conclusion: Skin disinfection methods were rather variable. Sampling interval, blood volume, and storage of bottles should be standardized. More than 10% of the hospitals require quality improvement in terms of positive rate and skin contamination rates. (Korean J Clin Microbiol 2011;14:91-96)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130510216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Multicenter Study of Molecular Epidemiology and Antibiotic Resistance of Group A Streptococci in 2008-2009 in Korea 2008-2009年韩国A群链球菌分子流行病学及耐药性多中心研究

Korean Journal of Clinical Microbiology Pub Date : 2011-09-01 DOI: 10.5145/KJCM.2011.14.3.85

E. Kim, Eun-Ha Koh, Sunjoo Kim, J. Kang, Jae Seok Kim, J. Shin, N. Lee, Joseph Jeong, J. Cho, Chulhun L. Chang, Young Ree Kim

引用次数: 3