International Journal of Sciences: Basic and Applied Research最新文献

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X-ray fluorescence spectrometric determination of heavy metals in selected rice samples sold on the Liberian market: a case study conducted in Paynesville City, Greater Monrovia x射线荧光光谱法测定利比里亚市场上出售的选定大米样品中的重金属:在大蒙罗维亚佩恩斯维尔市进行的个案研究
International Journal of Sciences: Basic and Applied Research Pub Date : 2020-07-02 DOI: 10.14419/IJBAS.V9I2.30754
M. James, Paye Plenseh Diana, N’debewillie Kokolo, K. P. C. Sampson, Kiazolu J. Boima
{"title":"X-ray fluorescence spectrometric determination of heavy metals in selected rice samples sold on the Liberian market: a case study conducted in Paynesville City, Greater Monrovia","authors":"M. James, Paye Plenseh Diana, N’debewillie Kokolo, K. P. C. Sampson, Kiazolu J. Boima","doi":"10.14419/IJBAS.V9I2.30754","DOIUrl":"https://doi.org/10.14419/IJBAS.V9I2.30754","url":null,"abstract":"Background: Environmental pollution with toxic heavy metals can be lead to the possible contamination of rice. Rice is a staple food widely consumed in the urban and rural parts of Liberia daily. Rice is cultivated in approximately 113 countries and a fundamental source for energy and protein. Objective: The study assesses selected heavy metals (As, Pb, Cd, Se, and Cr) concentration in selected imported rice and traditionally grown rice and bulgur wheat in Liberia. Methods: Six grade of imported rice, Bulgar wheat, and traditionally grown rice were purchased from the Duport Road and Red-Light markets in Greater Monrovia and analyze using X-ray Fluorescence Spectrometer. All data were analyzed using XLSTAT, and data was used to calculate the risk factor of each rice sample. Results: The mean concentration of heavy metal found in the rice as follow: As, 1.31ppm; Cd, 9.42ppm; Cr. 12.3ppm; Se, 5.73ppm; and Pb, 1.75ppm. The estimated daily intakes (EDIs) were calculated in combination with the rice consumption data. The mean intakes of As, Cd, Cr, Se, and Pb through rice were estimated to be 1.32, 9.42. 12.4. 5.74, and 1.75 mg/kg BW/day. Chromium has the average estimated daily intake. The combined hazard index for the heavy metals in each sample and the total cancer risk for each sample contributed most significantly to a cancer risk of rice consumption during adult life expectancy. Conclusion: The selected heavy metal concentration from the rice sample was above the FAO/WHO reference Standard but was within the range of the contaminant level except for chromium, which is above the accepted range. However, the hazard index and the total cancer risk indicate a potential non-carcinogenic and carcinogenic risk.","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"28 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77659191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Studies on diarrhea prevalence in selected communities in greater Monrovia, Liberia 利比里亚大蒙罗维亚选定社区腹泻流行情况研究
International Journal of Sciences: Basic and Applied Research Pub Date : 2020-06-01 DOI: 10.14419/IJBAS.V9I2.30570
James McClain, J. Boima Kiazolu, P. Humphrey, Plenseh Diana Paye
{"title":"Studies on diarrhea prevalence in selected communities in greater Monrovia, Liberia","authors":"James McClain, J. Boima Kiazolu, P. Humphrey, Plenseh Diana Paye","doi":"10.14419/IJBAS.V9I2.30570","DOIUrl":"https://doi.org/10.14419/IJBAS.V9I2.30570","url":null,"abstract":"Diarrhea is an epidemic that threatens the livelihood of children less than five years in developing countries. Control and mitigation pose a severe challenge in these countries. The subjective of the study is to assess the prevalence of and factors associated with diarrhea among families in Greater Monrovia. The study recruited 257 families from three communities and geographically and randomly assigned to the two groups (A & B). Socio-demographic survey and knowledge and behavior questionnaires on diarrhea prevalence were used to collect data. Reports from the study indicate that family in Group A (93%) and Group B (83.6%) have significant knowledge associating contaminated drinking water and contaminated food with diarrhea; X2 =11.2, p = 0.001. The family behavior shows that Group A (33%) and Group B (51%) do not treat their drinking water before consumption. The findings from this study recommend an education and awareness intervention on diarrheal and related illnesses to increase family knowledge and improvement of the behavior community public health improvement process.  ","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"24 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82025152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CONTRIBUTION TO THE IMPROVEMENT OF BITUMINOUS ENROBES: APPLICATION TO THE DEVELOPMENT WORKS OF THE YAOUNDE-DOUALA MOTORWAY IN CAMEROON 对改进沥青路面的贡献:在喀麦隆雅温得-杜阿拉高速公路发展工程中的应用
International Journal of Sciences: Basic and Applied Research Pub Date : 2020-05-11 DOI: 10.14419/IJBAS.V9I1.30269
Fabrice Mbakop Kwefeu
{"title":"CONTRIBUTION TO THE IMPROVEMENT OF BITUMINOUS ENROBES: APPLICATION TO THE DEVELOPMENT WORKS OF THE YAOUNDE-DOUALA MOTORWAY IN CAMEROON","authors":"Fabrice Mbakop Kwefeu","doi":"10.14419/IJBAS.V9I1.30269","DOIUrl":"https://doi.org/10.14419/IJBAS.V9I1.30269","url":null,"abstract":"The frequent closure of roads for the sake of maintenance and refurbishment is an increasing problem for the road administrators and users, because of induced high costs, restrictions and influence they impose, traffic jam, and the many other disruptions such interventions have on traffic. In these circumstances, carriageways with long life span, with adapted tar, have much to offer, especially if they can exhibit optimum performances without requiring major repairs for over 30 years. On high traffic roads, research has shown that binders with elevated modules are a reliable and credible alternative to render their life span more sustainable and durable. In this context, the advantages presented by the absence of important repairs and refurbishing can be enough to justify the initial high costs of these advanced carriageway coatings. Studies on the different binders to be used were done, which have permitted in accordance with the performance of the layers, the required tests and the different study levels. Also, this has permitted us to analyze the obtained results and conclude that due to the expected needs, the LA and MDE tests, the PSV tests are the criteria that determine the selection of aggregates; The complexes modular methods, fatigue resistance (level IV), is to be necessary for the forecasting of the mechanical behavior of bituminous materials.","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"5 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76825149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of perceived service quality using servqual mod-el: a case study of Pakistan international airline (PIA) in-flight hospitality 使用serval模型评估感知服务质量:巴基斯坦国际航空公司(PIA)机上接待的案例研究
International Journal of Sciences: Basic and Applied Research Pub Date : 2019-10-14 DOI: 10.14419/ijbas.v8i2.29714
Manzoor Ali Brohi, Aftab A. Shaikh
{"title":"Assessment of perceived service quality using servqual mod-el: a case study of Pakistan international airline (PIA) in-flight hospitality","authors":"Manzoor Ali Brohi, Aftab A. Shaikh","doi":"10.14419/ijbas.v8i2.29714","DOIUrl":"https://doi.org/10.14419/ijbas.v8i2.29714","url":null,"abstract":"The service industry is rapidly growing worldwide with latest and sophisticated tools and strategies for satisfaction of their ultimate customers. Provision of an exceptional service is the lifeblood of airline survival, with the airline’s mid-flight operations being the most crucial fragment of the whole service encounter. This study hence quantifies and weighs the perceived service quality against the desired service quality expected by the customers of Pakistan International Airlines during flight operations, through the 5 main dimensions of SERVQUAL exemplified by the 30 service attributes. The main objective of this study is to find out gap between perceived service quality and expected service quality.This research is quantitative in nature, Factor Analysis and one sample T-Test were used for analysis of data and close-ended questionnaires were used to collect the data from sample of 120 passengers who travelled through PIA at least once is last 12 months. According to the results shown by the Factor Analysis, 8 components were retained or extracted out of 30 variables; all the variables have high correlation with components as overall correlation measured by Factor Analysis is 0.804 which is excellent in the practice. According to KMO and Bartlett’s test, the sampling accuracy remained .857 which is quite good measurement. In addition to that, one sample T- test shows that variable Assurance scores 2.88 average mean perception out of 3.0 average mean expectation touches the expectations but not up to desire level of quality. However, other variables like responsiveness, Empathy, Reliability and Tangibility score 2.78, 2.67, 2.67, and 2.64 respectively.Average Mean Difference Gap (P-E) shows variable Assurance reveals low level of gap -0.12. However, this does not fill the quality gap of minimum 3.0 of Average Mean Expectation. This study exhibits tremendous service gap in other variables as Responsiveness -0.22, Empathy -0.33, Reliability -0.33 and Tangibility possesses -0.35 in terms of Average Mean Difference Gap.The necessity of this research study was deeply felt as a thin of number of passengers prefers PIA services. The service quality of PIA is diminishing with poor service strategies, insufficient facilities and lack of inflight hospitality measures for passengers; therefore, this research will help the key decision makers to identify the areas to improve, and take necessary remedial actions.  ","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81649566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Investigation of the relationship of the morphometric measurements of the hands and fingers with the personality traits of leadership 手、手指形态测量与领导人格特质关系的研究
International Journal of Sciences: Basic and Applied Research Pub Date : 2019-07-31 DOI: 10.14419/ijbas.v8i1.29310
Seda Sertel Meyvaci, R. Kosif, Murat Diramali, T. Baki
{"title":"Investigation of the relationship of the morphometric measurements of the hands and fingers with the personality traits of leadership","authors":"Seda Sertel Meyvaci, R. Kosif, Murat Diramali, T. Baki","doi":"10.14419/ijbas.v8i1.29310","DOIUrl":"https://doi.org/10.14419/ijbas.v8i1.29310","url":null,"abstract":"Background: The hand is a functionally critical organ at the distal end of the upper extremity. Also, the creases in the hands and the digital flexion creases on the fingers are the important external anatomical landmarks.Objectives: There are no studies found in the literature, linking solely the leadership personality traits with the anthropometric measurements of the hand.Methods: This descriptive study was to investigate the relationship between the 44 anthropometric measurements about hand, and the leadership personality traits in young adults from both genders.Results: When the leadership frames were compared by the scores, human resource leadership scores were significantly higher in the females. The charismatic leadership frame scores positively correlated with the parameters in males; including the breadth of the right hand, the breadth of the left hand, the index finger length of the left hand, and the distal phalanx length of the index finger on the left hand. The transformational leadership score was positively correlated with the left hand width and with the distal phalanx of the index finger on the left hand. In females, it was found out that the frames of human leadership and charismatic leadership correlated negatively with the length of the right thumb.Conclusions: According to the results of our study, we concluded that the breadth of the hand and the measurements of the thumb and the index fingers can provide opinion on leadership personality traits.  ","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85118111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Naphthoquinone impairs reproductive functions in plasmodium berghei berghei-infected male swiss mice 萘醌损害伯氏疟原虫感染的雄性瑞士小鼠的生殖功能
International Journal of Sciences: Basic and Applied Research Pub Date : 2019-05-05 DOI: 10.14419/IJBAS.V8I1.10355
N. Etukudoh, Akindele Oo, Kunle-Alabi Ot, A. Bolarinwa
{"title":"Naphthoquinone impairs reproductive functions in plasmodium berghei berghei-infected male swiss mice","authors":"N. Etukudoh, Akindele Oo, Kunle-Alabi Ot, A. Bolarinwa","doi":"10.14419/IJBAS.V8I1.10355","DOIUrl":"https://doi.org/10.14419/IJBAS.V8I1.10355","url":null,"abstract":"Background: Various antimalarial drugs adversely affect male reproductive functions. Naphtoquinones, a class of antimalarial drugs have been shown to effectively combat malaria parasites. However, the effects of naphtoquinone on reproductive functions remain elusive. The study determined the effects of naphthoquinone on reproductive functions of Plasmodium berghei berghei-infected male Swiss miceMethods: Thirty male Swiss mice were divided into 6 groups (n = 5) namely; Control, 1 mg/kg naphthoquinone, 2 mg/kg naphthoquinone, Plasmodium berghei berghei (Pbb)-infected, Pbb-infected+1 mg/kg naphthoquinone and Pbb-infected+2 mg/kg naphthoquinone. Parasitaemia was confirmed by microscopy. Naphthoquinone (i.p.) was administered for seven days following confirmation of parasitaemia. Thereafter, they were sacrificed. Serum levels of FSH, LH, testosterone and cortisol were assayed via ELISA. Sperm characteristics were evaluated by microscopy. Data were expressed as mean ± SEM and analysed using ANOVA at p<0.05.Results: Sperm motility reduced in the Pbb-infected compared with control. Sperm viability, motility and count reduced in naphthoquinone only groups and Pbb-infected+naphthoquinone groups compared with the control. Naphthoquinone groups and Pbb-infected alone decreased in LH and testosterone concentrations compared with the control.Conclusion: Naphthoquinone treatment impaired reproductive functions in Plasmodium berghei berghei-infected male Swiss mice.  ","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"336 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80626527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Assessing the Role of the Interleukin-12/STAT4 Axis in Breast Cancer by a Bioinformatics Approach. 用生物信息学方法评估白细胞介素-12/STAT4轴在乳腺癌中的作用
International Journal of Sciences: Basic and Applied Research Pub Date : 2019-01-01 Epub Date: 2019-08-18
Angel Núñez-Marrero
{"title":"Assessing the Role of the Interleukin-12/STAT4 Axis in Breast Cancer by a Bioinformatics Approach.","authors":"Angel Núñez-Marrero","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Interleukin-12 (IL-12) is an anti-tumor cytokine that promotes biological actions through the IL-12/STAT4 axis. Genetic variation and tumor microenvironment dynamics have been identified as critical elements for impaired immune anti-tumor responses. Breast cancer (BC) is a heterogeneous disease classified at the molecular level in several subtypes, each having unique biological and clinical traits. Despite research identifying the relevance of IL-12 in many cancer types, no studies have assessed the role of the IL-12/STAT4 axis in BC. The goal of this study was to evaluate the correlation of the IL-12/STAT4 signaling axis and BC patients' survival in general and in the context of the BC molecular subtypes. Bioinformatics analyses using TCGA data were completed to evaluate the correlation of the IL-12/STAT4 axis and BC. A high expression of important IL-12/STAT4 axis molecules such as the IL-12 receptor genes (<i>IL12RB1</i> and <i>IL12RB2</i>), <i>STAT4</i>, <i>IFNG</i> and <i>TBX21</i> were found to significantly increase BC patients' survival rates, especially in the most aggressive BC subtypes such as the luminal B (LumB), HER-2+ and basal like (BL). A possible relevant role of the IL-12/STAT4 axis in BC is suggested by this bioinformatics-study, which might also be subtype-specific. Further studies such as molecular and tumor microenvironment analyses will be required to clarify better the specific role of the IL-12 /STAT4 axis in BC. The results from these additional analyses may potentially improve IL-12-related immunotherapeutic approaches to BC.</p>","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"48 2","pages":"38-52"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253873/pdf/nihms-1585069.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37987088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Siri’s Marriage on Government Administration Siri的婚姻对政府管理的影响
International Journal of Sciences: Basic and Applied Research Pub Date : 2018-11-18 DOI: 10.31219/osf.io/yx3n7
M. R. R. Razak
{"title":"The Effect of Siri’s Marriage on Government Administration","authors":"M. R. R. Razak","doi":"10.31219/osf.io/yx3n7","DOIUrl":"https://doi.org/10.31219/osf.io/yx3n7","url":null,"abstract":"Impact or influence of siri marriage on government administration in Maiwa district, Enrekang Regency \". The research aims 1) To find out the siri marriage in Maiwa district 2) To find out government administration in Maiwa district 3) To find out the influence or impact of siri marriage on government administration in Maiwa District, Enrekang Regency. The method used is descriptive quantitative research with frequency table analysis techniques to determine the condition of independent and dependent variables namely siri marriage as a variable \"X\" and government administration as a variable \"Y\", while to know associative variables using Simple Linear Regression Analysis. The results showed that the siri marriage in Maiwa District was caused by adultery, divorce, polygamy and the number recorded in the last 3 years was 42 couples. Based on the tabulation of the frequency of government administration implementation in Maiwa district of 63.1; adultery, divorce marriage and polygamy have an influence on siri marriage at 69.8 percent while the influence of siri marriage on government administration using regression analysis is then processed using spss version 20.00 obtained R 2 or R square values from the summary table of 0.235 or 23.5 Siri marriage has an effect on government administration in Maiwa district, Enrekang Regency. While 76.5 percent is caused by other factors not examined.","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"7 1","pages":"171-184"},"PeriodicalIF":0.0,"publicationDate":"2018-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90110258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Entomophagy in traditional healthcare practiced by indigenous communities: potential, implications and constraints 土著社区传统保健中的食虫:潜力、影响和限制
International Journal of Sciences: Basic and Applied Research Pub Date : 2018-09-10 DOI: 10.14419/ijbas.v7i4.11434
Ruparao T. Gahukar
{"title":"Entomophagy in traditional healthcare practiced by indigenous communities: potential, implications and constraints","authors":"Ruparao T. Gahukar","doi":"10.14419/ijbas.v7i4.11434","DOIUrl":"https://doi.org/10.14419/ijbas.v7i4.11434","url":null,"abstract":"Several insect species including termites, honey bees, American cockroach, grasshoppers, stink bugs, aquatic bugs, silkworms and mopane caterpillars are popularly consumed by indigenous and tribal communities in South America, sub-Saharan Africa, South-east Asia and the Pacific. Different life stages of the insects were found effective in therapeutic treatment against health disorders and life-threatening diseases. In entomophagy (process of insect eating), allergy, content of anti-nutrients, contamination of micro-organisms, chemicals and biochemical compounds, and parasitic infection are major constraints for wide adoption of entomophagy. An urgent attention of policymakers, people’s organizations and researchers is therefore needed. This review is focused on worldwide utilization of selected edible insects for therapeutic treatment wherever modern medical facilities are not readily available or indigenous people prefer traditional healers. Certain practical safety measures against anticipated health risk are also elaborated.  ","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89060416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Optimization conditions of alkaline protease production by Streptomyces sp.H1 isolated from red sea coastal region in submerged culture 红海沿岸Streptomyces sp.H1深层培养产碱性蛋白酶的条件优化
International Journal of Sciences: Basic and Applied Research Pub Date : 2018-08-23 DOI: 10.14419/ijbas.v7i3.14519
Hind A. A. Al-Zahrani
{"title":"Optimization conditions of alkaline protease production by Streptomyces sp.H1 isolated from red sea coastal region in submerged culture","authors":"Hind A. A. Al-Zahrani","doi":"10.14419/ijbas.v7i3.14519","DOIUrl":"https://doi.org/10.14419/ijbas.v7i3.14519","url":null,"abstract":"A potent alkaline protease producing strain characterized and identified as Streptomyces sp. H1 was isolated from soil around red sea shore. The enzyme was produced extracellulary in submerged culture revealed maximum level during early stationary phase. Alkaline protease showed the highest activity at incubation time, pH and inoculum size of 3 days, 9 and 8% respectively. Among different carbon sources beet molasses gave a maximum production followed by starch, sucrose and fructose. High yield of protease production was noticed with casein followed by peptone, yeast extract and ammonium sulphate. Furthermore, it was optimized with 7g/l NaCl resulted in higher level of protease. Optimization of the process parameters resulted in about 3.4 fold increase in the alkaline protease. Partial purification of the crude enzyme was achieved by fractional precipitation using ammonium sulfate at 50% saturation. Due to the maximum production of protease in the presence of cheaper substrate as beet molasses, stability at alkaline pH 9 and temperature up to 70 oC besides salt tolerance make the strain and its enzyme useful in different industrial applications.  ","PeriodicalId":14296,"journal":{"name":"International Journal of Sciences: Basic and Applied Research","volume":"182 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74426431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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