{"title":"Silicone plastination of spinal cord of cat: as an alternative specimen for neuroanatomy education","authors":"O. Ekim, Hasen Awel Yunus, C. Bakıcı, Barış Batur","doi":"10.30704/http-www-jivs-net.1096113","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1096113","url":null,"abstract":"<jats:p xml:lang=\"tr\" />","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127648599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. S. Oran, Z. Yıldız, A. Temizyürek, Ayşin Selcan
{"title":"Effects of waiting time between trials and water temperature on cognitive functions, body temperature and body weight in rats in Morris water maze","authors":"D. S. Oran, Z. Yıldız, A. Temizyürek, Ayşin Selcan","doi":"10.30704/http-www-jivs-net.1115170","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1115170","url":null,"abstract":"The Morris water maze (MWM) is a widely used test among neurobiologists to measure spatial memory. The implementation of this test carries the risk of hypothermia periods in animals. The level of hypothermia may affect age-related memory processes as a significant factor. The occurrence of hypothermia throughout the MWM protocol should be better understood as hypothermia may impair memory performance. Ensuring the standardization of the experiments and minimizing side effects require a detailed examination of the hypothermia-related processes. Our study aims to replicate and extend the data of previous studies in terms of determining the possible species-specific variations and provide data for reorganizing the time intervals. In this study, rats (Wistar Hannover) were used and grouped according to the differences in the inter-trial interval (ITI) (30-s and 13-min) and water temperatures (20 °C and 24 °C). The effects of ITI and water temperature on probe performance were analysed statistically (mixed two-way ANOVA). Results showed that the 13 minute waiting group of animals performed statistically better in the MWM probe phase compared to the 30 second waiting group. The prolongation of ITI between the tests was found to have a positive impact on the memory performance. Longer ITI should be preferred instead of the frequently used 30-60 second test intervals. Thus, animals will be exposed to less stress and more reliable results can be obtained, also possible side effects of hypothermia can be minimized while performing the MWM test.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125755854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Minimally invasive plate osteosynthesis (MİPO) in veterinary orthopedics","authors":"Çağıl ÇOKCOŞKUN YILMAZ, C. Yardımcı","doi":"10.30704/http-www-jivs-net.1111320","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1111320","url":null,"abstract":"The current increase in the numbers of fracture treatment by plate osteosynthesis in veterinary medicine is leading to the production of specific plates for different types of fractures. Recent studies about fracture healing show that MIPO procedure is superior for faster union and healing by decreased contamination risk, faster return of function, lower complication rates and blood supply preservation. By now, indirect reduction technics are more valuable in preservation of the biological structure of bone than full anatomic reduction techniques. Day by day, MIPO becomes more popular in veterinary orthopedics. Basicly the method is applying a plate without opening the fractured area to make a bridging between the proximal and distal metaphysis/diaphysis of the fragments. The success of the procedure relies on the type of the fracture and the fracture area. The procedure can be applied especially diaphyseal segmental fractures with success but to be avoided in articular fractures. The procedure has been being used usually in the diaphyseal tibial and radial fractures of the cats and dogs. But nowadays it has started to be used in femoral and humeral fractures as well. The disadvantages of the procedure is the difficulty of the application and the need of the intraoperative radiography or fluoroscopy for the correct positioning of the fractures.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121758759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Electron microscopy and histopathological examination of canine papilomavirus","authors":"B. K. Tekelioglu","doi":"10.30704/http-www-jivs-net.1106150","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1106150","url":null,"abstract":"Diagnosis of canine papillomavirus (CPV) infection by histopathology, transmission and scanning electron microscopy is presented. The study is based on data obtained by examining nonregressing papillomas (warts) from naturally infected dogs with clinical manifestations of CPV infection. Papules on the mouth and lips were common bilaterally in all dogs. Confirmatory diagnosis of sick dogs was made by clinical findings, histopathology, transmission and scanning electron microscopy. Histopathological examination of hematoxylin and eosin stained papillomas revealed lymphoplasmocytic cell infiltration and fibrosis, parakeratosis in the dermis, papillary proliferation and intranuclear vacuole degeneration in the stratum spinosum. Electron microscopy demonstrated viral icosahedral capsid formation and non-enveloped viral structure of CPV. Transmission electron microscopy demonstrated viral particles and virions in the nuclei of infected cells, viral crystal mode formation in the nucleus. Scanning electron microscopy demonstrated virions and virus-like particles budding in the infected tissue. The findings of the study reveal that electron microscopy and histopathology are effective and sensitive methods in the diagnosis of CPV infection. Electron microscopy is the only imaging technique that allows direct visualization of viruses, along with affected tissues and cells, due to its nanometer-scale resolution. This study reveals the intracellular and extracellular viral pathogenesis, viral ultra structure and structural components of CPV. Present findings indicate canine papillomavirus causes canine papillomatosis, inclusion bodies are common in nonregressive infection, papillomavirus induces cytopathic effect and pathogenesis, viral particles located in the cell and forms crystal mode in nuclear space.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125600065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bayram Süzer, N. Seyidoglu, K. Tüfekci, Deniz Karakci, B. Bakır
{"title":"A Preventive herb against bone loss in diabetic rats: Zingiber officinale","authors":"Bayram Süzer, N. Seyidoglu, K. Tüfekci, Deniz Karakci, B. Bakır","doi":"10.30704/http-www-jivs-net.1120974","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1120974","url":null,"abstract":"The study aims to determine and compare bone mechanical and material properties in experimentally diabetic rats treated with ginger extract. Forty female, healthy Wistar albino rats were used in the study. Rats were divided into five groups; Control (C), Sham (S), Ginger (G), Diabetic (D), and Diabetic rats treated with Ginger (DG). Diabetes mellitus was induced by a single intraperitoneal injection of 50 mg/kg streptozotocin. Ginger-treated rats received 200 mg/kg ginger extract by oral gavage in a 30-day-trial. At the end of the study, tibiae were harvested and subjected to a three-point bending test. Plasma samples were also analyzed for calcium and phosphorus concentrations. It was observed that the bending strength significantly decreased in the groups Ginger (234.78 ± 16.79; P = 0.019) and the Diabetic (223.90 ± 29.90; P = 0.028) compared to group Control (275.75 ± 33.47). In addition, the bending strength of the diabetic rats treated with ginger (DG group; 251.92 ± 15.90) was also significantly higher than the rats in the Ginger and Diabetic groups (P = 0.032 and P = 0.037, respectively). Although the plasma calcium concentrations showed no differences among any of the groups, the plasma phosphorus levels decreased significantly in group Diabetic (3.47 ± 0.28; P = 0.05) compared to Control (5.11 ± 0.21). However, there was a significant increase in plasma phosphorus in group DG (4.32 ± 0.12; P = 0.05) compared to Diabetic. In conclusion, ginger extract treatment of diabetic rats improves bone material properties. The adverse effects of diabetes on the mechanical properties of the bone were prevented by using ginger extract in diabetic rats.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134508432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"How do viruses use oxidative stress?","authors":"Ece Adıgüzel, T. Oğuzoğlu","doi":"10.30704/http-www-jivs-net.1117825","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1117825","url":null,"abstract":"Oxygen is a vital element for all living beings to continue their life activities and is the main component of oxidant–antioxidant metabolism, which should be in balance. The free radicals formed as a result of this metabolic process in the organism constitute a source of oxidants; external factors (radiation, exposure to sunlight, environmental pollution, cigarettes, etc.), inflammation and microbial agents also cause the formation of oxidants. Oxidative stress occurs when the balance between free radicals and antioxidants (which have an eliminating effect against them) shifts in favour of free radicals. Many studies have reported that oxidative stress may affect the virulence of pathogens during infection. Viruses use a pathological pathway that causes the production of reactive oxygen species (ROS) and the consumption of antioxidants. Thus, after viral infections, higher levels of ROS are often formed. Not only DNA-containing but also RNA-containing viruses were found to be associated with severe oxidative stress supporting DNA damage, high mutagenicity, initiation and/or progression of neoplasia. This review focuses on the relationship between oxidative stress and viruses.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"226 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114160134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of capsaicin on TBARS and TAS in rats with hypothyroidism","authors":"Fatih Erteki̇n, T. Keçeci","doi":"10.30704/http-www-jivs-net.1137848","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1137848","url":null,"abstract":"In this study, capsaicin was administered to rats with experimental hypothyroidism. İt was aimed to determine the changes in plasma levels of thiobarbituric acid reactive substances (TBARS), which are indicators of oxidative stress, and total antioxidant capacity (TAS), which is one of the components of antioxidant defence mechanisms. A total of 32 healthy male Wistar Albino rats weighing 300-350 g, approximately 12 weeks old, were used as animal material in the study. Rats were divided into four equal groups control (K), Capsaicin (C), Hypothyroid (H) and capsaicin + Hypothyroid (CH). During the 30-day trial period, (10mg / kg / day) capsaicin was administered to the rats in group C by oral gavage per animal. In group H, 6-n-propyl-2-thiouracil (PTU) was added daily to their drinking water at 0.05% weight/volume (W/V). In the CH group, 10 mg/kg/day of capsaicin was administered by oral gavage method and 0.05% weight / volume (W / V) of PTU was added to drinking water. At the end of the application, we obtained plasma and serum samples from the subjects in the groups under general anaesthesia (thiopental anaesthesia, 40 mg/kg) and by taking sufficient amount of blood from the heart by cardiac puncture. We determined thyroid-stimulating hormone (TSH), total triiodothyronine (TT3), free T3 (fT3), total thyroxine (TT4) and free T4 (fT4) levels from serum samples, and TBARS and TAS levels from plasma samples. In conclusion, in the light of the data obtained in this study, we determined that lipid peroxidation and oxidative stress occur in hypothyroidism. However, we concluded that the application of capsaicin is partially sufficient to maintain the oxidant/antioxidant balance.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116227721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Trends towards the use of natural anesthetics in fish","authors":"T. Ö. Yaşar, M. Yardımcı","doi":"10.30704/http-www-jivs-net.1089008","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1089008","url":null,"abstract":"","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116566923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Feline fibroepithelial hyperplasia and current treatment protocols","authors":"Hülya Kula, Zeynep Günay Uçmak","doi":"10.30704/http-www-jivs-net.1031677","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1031677","url":null,"abstract":"","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127953442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of mating method and mating season on the number of puppies and determination of some reproductive parameters in Pomeranian dogs","authors":"M. Özmen","doi":"10.30704/http-www-jivs-net.1052870","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.1052870","url":null,"abstract":"","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134052155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}