International journal of peptide and protein research最新文献_第3页

Side-reactions in peptide synthesis. 2. Formation of by-products in the large-scale synthesis of THF-gamma 2 by solution synthesis. 肽合成中的副反应。2. 溶液合成大规模合成thf - γ - 2的副产物的形成。

International journal of peptide and protein research Pub Date : 1994-06-01

R Forino, M Galantino, F Ciprandi, L Baumer, E Arlandini, R De Castiglione

引用次数: 0

Some 'difficult sequences' made easy. A study of interchain association in solid-phase peptide synthesis. 一些“困难的序列”变得简单。固相肽合成中链间结合的研究。

International journal of peptide and protein research Pub Date : 1994-05-01

C Hyde, T Johnson, D Owen, M Quibell, R C Sheppard

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Synthesis of disulfide-bridged fragments of omega-conotoxins GVIA and MVIIA. Use of Npys as a protecting/activating group for cysteine in Fmoc syntheses. - omega- concontoxins GVIA和MVIIA二硫化物桥接片段的合成。Npys在Fmoc合成中作为半胱氨酸保护/激活基团的应用。

International journal of peptide and protein research Pub Date : 1994-04-01

R G Simmonds, D E Tupper, J R Harris

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N-9-fluorenylmethoxycarbonylpyroglutamate. Preparation of the acid, chloride and succinimidyl ester. N-9-fluorenylmethoxycarbonylpyroglutamate。酸、氯和琥珀酰亚胺酯的制备。

International journal of peptide and protein research Pub Date : 1994-04-01

N L Benoiton, F M Chen

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Potent pseudopeptide bombesin-like agonists and antagonists. Correlation of ordered conformation of bombesin analogs to receptor activity. 强效假多肽炸弹蛋白样激动剂和拮抗剂。bombesin类似物的有序构象与受体活性的相关性。

International journal of peptide and protein research Pub Date : 1994-04-01

J V Edwards, L R McLean, A C Wade, S R Eaton, E A Cashman, K A Hagaman, B O Fanger

{"title":"Potent pseudopeptide bombesin-like agonists and antagonists. Correlation of ordered conformation of bombesin analogs to receptor activity.","authors":"J V Edwards, L R McLean, A C Wade, S R Eaton, E A Cashman, K A Hagaman, B O Fanger","doi":"","DOIUrl":"","url":null,"abstract":"Bombesin-like pseudopeptides have been synthesized, and certain physicochemical properties and biological activities have been examined. Bombesin and the related peptide litorin were modified at positions 13-14 and 8-9, respectively, with psi[CH2S] and psi[CH2N(CH3)]. [Phe13 psi[CH2S]Leu14]bombesin and [Phe8 psi[CH2S]-Leu9]litorin bound to the murine pancreatic bombesin/gastrin releasing peptide receptor with similar dissociation constants (Kd = 3.9 and 3.4 nM, respectively). Increased potency was achieved by oxidation of the thiomethylene ether to two diastereomeric sulfoxides (isomer I, Kd = 1.6 nM and isomer II, Kd = 0.89 nM. Further oxidation to the sulfone decreased potency ([Phe8 psi[CH2SO2]Leu9]litorin, Kd = 9.9 nM). All five analogs were receptor antagonists as determined by phosphatidylinositol turnover in murine pancreas. In contrast to these peptide backbone substitutions, a psi[CH2N(CH3)] at the 8-9 amide bond position resulted in an agonist. The analogs were compared with those of litorin (Kd = 0.1 nM) and [Leu9]litorin (Kd = 0.17 nM) by CD and fluorescence spectroscopy. The CD spectra demonstrated ordered conformation for all the peptides in TFE. Different conformations corresponding to agonist and antagonist peptides were suggested by CD. Based on the pH-dependence of the fluorescence spectra of the peptides in a zwitterionic detergent, two titratable groups were identified (pKa = 6.3 and 8.5). The lower pKa is found in the agonist analogs but not in the psi [CH2S]-containing antagonist.","PeriodicalId":14204,"journal":{"name":"International journal of peptide and protein research","volume":"43 4","pages":"374-83"},"PeriodicalIF":0.0,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19043033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of an aspartic acid-containing protected peptide. Alternatives for overcoming a side reaction during HF treatment. 含天冬氨酸保护肽的制备。克服心衰治疗期间副反应的替代方法。

International journal of peptide and protein research Pub Date : 1994-04-01

J Robles, E Pedroso, A Grandas

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Harnessing D-amino acids for peptide motif designs. Synthesis and solution conformation of Boc-D-Glu-Ala-Gly-Lys-NHMe and Boc-L-Glu-Ala-Gly-Lys-NHMe. 利用d -氨基酸进行肽基序设计。Boc-D-Glu-Ala-Gly-Lys-NHMe和Boc-L-Glu-Ala-Gly-Lys-NHMe的合成及溶液构象

International journal of peptide and protein research Pub Date : 1994-03-01

V Bobde, Y U Sasidhar, S Durani

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The concept of superactive esters. Could peptide synthesis be improved by inventing superactive esters? 超活性酯的概念。发明超活性酯能改善肽合成吗?

International journal of peptide and protein research Pub Date : 1994-03-01

Z J Kamiński

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Pegylated peptides. II. Solid-phase synthesis of amino-, carboxy- and side-chain pegylated peptides. 聚乙二醇缩氨酸。2氨基、羧基和侧链聚乙二醇肽的固相合成。

International journal of peptide and protein research Pub Date : 1994-02-01

Y A Lu, A M Felix

{"title":"Pegylated peptides. II. Solid-phase synthesis of amino-, carboxy- and side-chain pegylated peptides.","authors":"Y A Lu, A M Felix","doi":"","DOIUrl":"","url":null,"abstract":"General procedures are presented for the site-specific pegylation of peptides at the NH2-terminus, side-chain positions (Lys or Asp/Glu) or COOH-terminus using solid-phase Fmoc/tBu methodologies. A model tridecapeptide fragment of interleukin-2, IL-2(44-56)-NH2, was chosen for this study since it possesses several trifunctional amino acids which serve as potential sites for pegylation. The pegylation reagents were designed to contain either Nle or Orn, which served as diagnostic amino acids for confirming the presence of 1 PEG unit per mole of peptide. NH2-Terminal pegylation was carried out by coupling PEG-CH2CO-Nle-OH to the free NH2-terminus of the peptide-resin. Side-chain pegylation of Lys or Asp was achieved by one of two pathways. Direct side-chain pegylation was accomplished by coupling with Fmoc-Lys(PEG-CH2CO-Nle)-OH or Fmoc-Asp(Nle-NH-CH2CH2-PEG)-OH, followed by solid-phase assemblage of the pegylated peptide-resin and TFA cleavage. Alternatively, allylic protective groups were introduced via Fmoc-Lys(Alloc)-OH or Fmoc-Asp(O-Allyl)-OH, and selectively removed by palladium-catalyzed deprotection after assemblage of the peptide-resin. Solid-phase pegylation of the side-chain of Lys or Asp was then carried out in the final stage with PEG-CH2CO-Nle-OH or H-Nle-NH-(CH2)2-PEG, respectively. COOH-Terminal pegylation was achieved through the initial attachment of Fmoc-Orn(PEG-CH2CO)-OH to the solid support, followed by solid-phase peptide synthesis using the Fmoc/tBu strategy. The pegylated peptides were purified by dialysis and preparative HPLC and were fully characterized by analytical HPLC, amino acid analysis, 1H-NMR spectroscopy and laser desorption mass spectrometry.","PeriodicalId":14204,"journal":{"name":"International journal of peptide and protein research","volume":"43 2","pages":"127-38"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19191940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

X-ray studies on crystalline complexes involving amino acids and peptides. XXVI. Crystal structures of two forms of L-histidine acetate and a comparative study of the amino acid complexes of acetic acid. 氨基酸和多肽晶体配合物的x射线研究。第二十六章。两种形式的l -组氨酸醋酸酯的晶体结构及乙酸氨基酸配合物的比较研究。

International journal of peptide and protein research Pub Date : 1994-02-01

S Suresh, G S Prasad, M Vijayan

{"title":"X-ray studies on crystalline complexes involving amino acids and peptides. XXVI. Crystal structures of two forms of L-histidine acetate and a comparative study of the amino acid complexes of acetic acid.","authors":"S Suresh, G S Prasad, M Vijayan","doi":"","DOIUrl":"","url":null,"abstract":"L-Histidine acetate crystallizes in two forms: (I) orthorhombic; P2(1)2(1)2(1); a = 5.027, b = 11.126, c = 17.473 A; Z = 4; (II) monoclinic; C2; a = 15.649, b = 9.276, c = 8.566 A; beta = 94.65 degrees; Z = 4. The structures were solved by direct methods and refined to R-values of 0.056 and 0.089 for 1131 and 1330 observed reflections, respectively. The conformations of the histidine molecule in the two forms are different. However, both are such that they facilitate the occurrence of a specific interaction of the histidine molecule with a carboxylate group. The basic elements of aggregation are hydrogen-bonded histidine ribbons, but they are of different types in the two structures. The ribbons are interconnected by acetate ions to form the crystals. The structures contain two characteristic interaction patterns involving amino and carboxylate groups, one of which is observed for the first time. The two water molecules in form II and their symmetry equivalents form an uninterrupted hydrogen-bonded chain running through the crystal. They also present an interesting case of disorder in hydrogen bonds. A comparative study involving amino acid complexes of acetic acid shows that the presence of acetate ion could lead to new aggregation patterns, specific interactions and characteristic interaction patterns with varying degrees of similarity with those observed in other structures containing amino acids.","PeriodicalId":14204,"journal":{"name":"International journal of peptide and protein research","volume":"43 2","pages":"139-45"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19191941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0