International Journal of Life Science Research Archive最新文献

{"title":"Study on hemostatic activities of Aspilia africana (Pers.) C. D. Adams and Conyza canadensis L.","authors":"Akanji Olufunke Christy","doi":"10.53771/ijlsra.2023.4.1.0021","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0021","url":null,"abstract":"The process of hemostasis involves the body turning blood into a thick, gel-like mass at the site of injury or damage in order to stop bleeding. Africa and Latin America are the native habitats of Aspilia africana (Family Asteraceae). Hemorrhage plant and wild Sunflower are frequent names for this plant, which grows in savanna wastelands and forested areas. Conyza canadensis (Family Asteraceae) is commonly known as Canadian horseweed or Canadian fleabane. It is present in all warm nations, but because of its global distributions from America, it is thought to have originated there and spread to the rest of the world. This study investigated hemostatic activities of the leaves extract of Aspilia africana and Conyza canadensis on mice. Fresh leaves of the plant samples were extracted with methanol and thirty-two (32) mice grouped into eight of four mice were used for the study. Incision was made on the tail of each mouse. Warfarin, a standard drug (3 mg/kg), was used as positive control while each plant extract was made into concentrations of 50, 100 and 200 mg/kg. The bleeding time (seconds) of breaking tail and volume of bleeding were measured to evaluate the hemostatic activity after administration of treatments for 5 days (once per day). The results of this study showed reduction in the volume of bleeding in all the treatment groups when compared to untreated (negative control) group but highest activity was recorded in warfarin (Positive control). Aspilia africana (200 mg/kg) methanol extract (1.12±0.01) was not statistically different from the mean values of warfarin (1.11±0.01). The results of bleeding time (Seconds) in tails of the mice of warfarin, Aspilia africana methanol extract and Conyza canadensis methanol extract groups reduced when compared to the negative control group, but Aspilia africana extract gave highest activity at 200 mg/kg. The significant ability of Aspilia africana leaves extract to effectively arrest bleeding from fresh wounds and accelerate wound healing process might be attributed to the metabolites present. However, further studies should be carried out on its toxicity and mechanism of actions.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"33 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80138160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implication of exon 4 TP53 Gene mutations in colorectal cancers in Senegalese patients","authors":"Anna Ndong, Bineta Keneme, Fatimata Mbaye, Mbacké Sembène","doi":"10.53771/ijlsra.2023.4.1.0092","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0092","url":null,"abstract":"Background: Colorectal cancer (CRC) is a major cause of death in men and women, comprising about 10% of cancer deaths after breast and lung cancer. The most significant risk factors currently implicated in the etiology of this cancer are genetic, likely due to several mutations. Methods: The study explored the involvement of genetic factors in these pathologies; specifically, exon 4 of the TP53 gene was studied in 15 CRC patients and compared with 10 healthy individuals as controls. The position of the marker mutations was determined with the Mutation Surveyor software version 5.1.2. DnaSP version 5.10, MEGA version 7.014, and the program Arlequin version 3.1 were used to highlight the parameters of variability, differentiation, and the demo-genetic evolution of our study populations. The pathogenicity of the mutations was determined through Polyphen2, TAMISER, and ClinVar. Results: Our results showed the presence of a recurrent mutation of the TP53 gene in both tumor and healthy tissue where proline was replaced with arginine at codon 72. This mutation was predicted to be benign. The presence of this mutation in healthy tissue can be considered a relatively late event in colorectal tumorigenesis. In addition, the P47L, D49A, W53S, and D48G mutations appeared to be suspicious because they were predicted to be potentially damaging. This finding suggests the genes’ involvement in the pathology of CRCs in our study population. The cancer tissue sequences contained an average of 2.59 nucleotide differences that resulted in amino acid changes. The Nei genetic distance confirms this variability between tumor tissues. Conclusion: These results suggest that variants in exon 4 of the TP53 gene may contribute to the development of CRCs. These mutations could constitute molecular markers in CRC and possibly help in the development of early diagnosis","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91369236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycyrrhiza glabra assisted green synthesis of metallic nanoparticles with different applications: A review","authors":"Rakshanda Bairagi, Shriniwas Patil","doi":"10.53771/ijlsra.2023.4.1.0157","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0157","url":null,"abstract":"Several attempts have been made for green synthesis of nanoparticles of different metals, revealing the significance of plant extract in reducing metal source to nanoparticles and applications in various scientific domains. This article focus on the applications on licorice root extract in synthesis of nanoparticles of various metals like gold, zinc, silver etc. Glycyrrhiza glabra is a general herb of the Asian region belonging to family fabaceae and it is commonly used in the diet. Roots of this plant show range of activities including antioxidant, antimutagenic level. And respective research attempts, this metallic nanoparticles were evaluated for one or more applications like anticancer, antibacterial, antioxidant activity etc. Use of Glycyrrhiza glabra polar extract indicated involvement of its polar phyto-compounds in reducing the metal source as stabilizing the nanoparticles. In conclusion it could be noted that metal nanoparticles have better antimicrobial, antioxidant, anticancer and nematicidal potential over the aqueous root extract.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85069276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Septicemia and meningitis caused by group A streptococcus: A case report and literature review","authors":"Sara Kouara, Wiame Ghammad, Selma Berrada, Mustapha Mahmoud, Ghita Yahyaoui","doi":"10.53771/ijlsra.2023.4.1.0156","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0156","url":null,"abstract":"The invasive diseases due to Streptococcus pyogenes are pretty rare infections especially in pediatrics. We present to you a case of a 13-year-old who was admitted in pediatric’s intensive care for a polytrauma, on his 4th day of hospitalization he developed a fever for which he had a cytobacteriological assay of the spinal fluid who was in favor of a bacterial meningitis with 61 white cells predominantly neutrophilic 81% and a positive direct examination and culture after 24-hour incubation. The blood cultures from 3 sets (5 bottles) were also positive after 24h incubation and all pointed out to β hemolytic colonies in favor of streptococcus that was identified afterwards with serogrouping and on an automaton proving the incrimination of streptococcus group A. A review of literature was then conducted to enlighten clinicians on this unusual but equally dangerous bacteria.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85071651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teaching, learning and assessment of professionalism in medical education: A citation-based systematic review of literature","authors":"Firdous Jahan, Nisar Ahmad, Ikram A Burney","doi":"10.53771/ijlsra.2023.4.1.0155","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0155","url":null,"abstract":"Background: Professionalism is an important competence in medical practice, yet a complex area. We describe the landscape of published articles on teaching, learning and assessment of professionalism in medical schools. This study aims to review the temporal trend of literature published and cited on the subject, and includes frequently publishing authors, journals, and network analysis. The study further aims to present the themes of the most commonly cited articles. Methods: A systematic review of peer-reviewed literature was carried out to describe the current patterns of research in professionalism using the terms “professionalism” AND \"medical education\" OR \"medical college\" OR \"medical school\". The title and abstract of the selected documents were skimmed to include only relevant articles. ‘Relevant’ articles were defined as articles describing teaching, learning or assessment of professionalism in undergraduate medical curricula. Articles describing professionalism, but not directly related to teaching, learning and assessment were excluded. Results: Over the study period spanning from 1973 to 2020, a total of 1053 articles were identified as relevant. A surge in the number of publications was observed from the year 2000. Over 400 articles were published in 5 leading journals in medical education. Of the 50 top-cited papers, 18 (36%) articles explored opinions of stake holders through questionnaires and surveys about different aspects of professionalism, there were 13 (26%) ‘Commentary’ and 7 (14%) review articles. The primary objectives of more than 50% of the top-cited articles were assessment methods (14%), teaching by humiliation (14%), personal identity formation (8%), barriers to professionalism (8%), and teaching ethics (8%) and empathy (8%). Conclusions: We describe the landscape of published articles on teaching, learning and assessment of professionalism in undergraduate medical schools. The surge in publications since 2000, top publishing journals and frequently publishing authors were identified. Of the most cited articles, assessment methods, personal identity formation, and teaching empathy and ethics predominate as the main themes.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83521033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequence comparison of the lactoferrin of various animal species and its prospects as an animal feed additive","authors":"Hamong Suharsono, Ni Luh Wayan Yulia Mirayanti, Nengah Kerta Besung, Ni Putu Sutrisna Dewi, Ni Putu Novi Aritayanti, Made Sumitha Kameswari, Bayu Krisna Mahardika, Ida Bagus Kade Suardana, Xiaole Qi, Gusti Ngurah Mahardika","doi":"10.53771/ijlsra.2023.4.1.0011","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0011","url":null,"abstract":"Lactoferrin as a feed additive is worth to be explored. However, reports on its application are contradictory, which might be associated with the genetic relatedness. Here we compare the lactoferrin nucleotide and its deduced amino acid sequence. Sequence data for lactoferrin of various animal and human were download from GenBank. The phylogenetic relatedness was inferred and the amino acid sequences were aligned to identify conserved and polymorphic sites. The protein three-dimension structures were estimated using online software. The result showed the lengths of lactoferrin, lactotransferrin or ovotransferrin are 703–711 residues. The phylogeny showed that the lactoferrins of buffalo, cow, goat, pig, camel, and horse formed one group; monkey, human, and gorilla formed a second group; and dog and cat formed a third group. Chicken ovotransferrin was an outgroup. Genetic distances between groups were 0.242–0.061, while smallest span between taxa was 0.016 (human to gorilla) and the highest was 0.612 (chicken to goat). The conserved residues spanned from the amino terminus to the carboxy terminus. There are 27 conserved cysteine residues. N-link glycosylation of the “NXS” and “NXT” motives of lactoferrin diverge between species. A species specific or group specific lactoferrin supplement should be beneficial to animal production.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90675180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first report of syllable analysis of the chattering lory (Lorius Garrulus (Linnaeus, 1758)) in the crooked beak sanctuary of Koli Village, Tidore Islands City, North Maluku","authors":"Abdu Mas’ud, Ningsi Saibi, Sundari","doi":"10.53771/ijlsra.2023.4.1.0158","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0158","url":null,"abstract":"The Chattering lory is an endemic bird with a restricted range in North Maluku. The crooked-beaked bird is one of the world's most popular types of domesticated birds, which negatively affects its long-term viability. The population estimate for chattering lory birds is between 46,360 and 295,540 heads. Poaching is the most significant factor contributing to population decline. An estimated 16,000 chattering lory are captured and sold annually. This study intends to analyze the syllables of bird sounds, as bird-sound-related research is still uncommon. The data collected from a Voice Recording of the chattering lory (Lorius garrulus) was then analyzed using Avisoft-SASlab Lite software. This study was conducted by recording bird sounds. The recording process (Analog Recording) is carried out within a specific time interval to record the birds' sounds multiple times. Bird sounds are recorded in the morning (07:00 to 10:00 WIT). The results indicated that the chattering lory's 131.4-second-long call consists of 18 syllables and four elements. The duration of a syllable varies from 0.51 to 0.66 seconds, and the duration between syllables ranges from 1.31 to 16.09 seconds. Also, the variable is the frequency of each upper and lower element. The lower element's frequency ranges from 100 to 12,100 kHz, while the upper element's frequency ranges from 1,500 to 18000 kHz.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88676446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A concomitant infection of leptospirosis and filariasis","authors":"Rajini KV, Vinay Khanna","doi":"10.53771/ijlsra.2023.4.1.0137","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0137","url":null,"abstract":"Leptospirosis is a bacterial zoonosis & filariasis is a tropical disease. The causative agent of leptospirosis can be detected in blood, CSF, and urine depending upon the disease progression, while microfilariae are detected in peripheral blood, generally collected during the night. Both diseases are of significant concern as they can lead to serious conditions if untreated at the right time. Here we reported a case of a patient with leptospirosis & filariasis.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82714785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the effectiveness of the sanitization activity of a robot equipped with UV lamps","authors":"Dario Russignaga, Luca Maria D’apuzzo, Matteo Nazzario, Irene Borgini, Simone Pescarolo, Giorgio Gilli","doi":"10.53771/ijlsra.2023.4.1.0013","DOIUrl":"https://doi.org/10.53771/ijlsra.2023.4.1.0013","url":null,"abstract":"The increasing focus on the health of individuals has forced a strong acceleration on research and development of new technologies to abate the microbiological component. The pandemic generated by the spread of the SARS-CoV-2 virus has imparted an even greater impetus in this direction. In large and busy environments, it is essential to implement existing air purification and surface cleaning techniques, to reduce the concentrations of microorganisms present as much as possible: for the abatement of airborne bacterial loads, physical methods (e.g., HEPA filters) are used, while, for surfaces, chemicals of different compositions (detergents and bactericides) are often used. The use of ultraviolet radiation is proving to be a very viable alternative for this purpose. The use of UV lamps coupled with automated systems allow constant service to prevent the spread of microbiological populations. The study reported here aims to verify the features of the bactericidal action produced by the ARIS-K2 robot. This specific machine is equipped with 6 UV-C mercury lamps, which emit electromagnetic radiation at a wavelength value of 253.7 nm. Ultraviolet light is divided into three categories, depending on the wavelength: UV-A (315-400 nm), UV-B (280-315 nm) and UV-C (100-280 nm). UV-C radiation is demonstrated to have the greatest microbicidal effect. The conducted study analyzes the survival of bacterial populations distributed on surfaces at varying distances from the robot and in the presence of obstacles. The results were also analyzed considering the dose-exposure time relationship to which the colonies were subjected.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82832999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of HBsAg among patients attending a tertiary hospital in Port Harcourt, Nigeria","authors":"Ifeoma Tochi Cookey, Kenneth Chukwuma Odenigbo, Blessing Jachinma Okonko, Iheanyi Omezuruike Okonko","doi":"10.53771/ijlsra.2022.3.2.0124","DOIUrl":"https://doi.org/10.53771/ijlsra.2022.3.2.0124","url":null,"abstract":"Nigeria has one of the greatest disease burdens from chronic viral hepatitis. Hepatitis B virus (HBV) infection is an important public health problem worldwide, more than two million people. Globally, around 96% of viral hepatitis deaths are attributable to HBV and Hepatitis C virus (HCV) but the prevalence of these infections is poorly characterized. Thus, this study aimed to investigate seroepidemiological aspects of HBV infection and its associated factors among patients attending a tertiary hospital in Port Harcourt, Nigeria. Serum samples from 92 participants were screened for a serological marker of HBV infection (HBsAg) by Monolisa HBsAg ULTRA enzyme-linked immunosorbent assay (ELISA) kit (manufactured by BIO RAD Laboratories, California, United States) following the manufacturer’s guidelines. Of the 92 patients tested, 10 (10.9%) were positive while 82 (89.1%) were negative. Among them (10/92), the majority were females (11.0%, 8/73) than males (10.5%, 2/19); the highest prevalence rate of an HBsAg occurred in the age group of 21-30 years (17.6%, 3/17). A higher prevalence of HBV (13.2%, 9/68) occurred in the married than singles (4.2%, 1/24). This study showed a high seroprevalence (10.9%), which indicates a rise in the prevalence of HBV in the study area. The findings of this study confirm the findings of other studies that HBV is endemic in Nigeria. These finding views females and males as fairly equivalent in the risk of HBV infection. As the prevalence of young adults were higher than older adults, this is considered cogent as younger adults are implicated as risk factors for HBV infection. This study adds important granularity to our understanding of the hepatitis epidemic. Large cross-sectional studies are needed to better characterize HBV prevalence, but mass screening may not be warranted.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76729177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}