ACS Chemical Neuroscience最新文献_第10页

Rapid Microwave Fixation of the Brain Reveals Seasonal Changes in the Phosphoproteome of Hibernating Thirteen-Lined Ground Squirrels

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-22 DOI: 10.1021/acschemneuro.4c0063510.1021/acschemneuro.4c00635

Md Shadman Ridwan Abid, Michael J. Naldrett, Sophie Alvarez, Catherine D. Eichhorn, Matthew T. Andrews* and James W. Checco*,

{"title":"Rapid Microwave Fixation of the Brain Reveals Seasonal Changes in the Phosphoproteome of Hibernating Thirteen-Lined Ground Squirrels","authors":"Md Shadman Ridwan Abid, Michael J. Naldrett, Sophie Alvarez, Catherine D. Eichhorn, Matthew T. Andrews* and James W. Checco*, ","doi":"10.1021/acschemneuro.4c0063510.1021/acschemneuro.4c00635","DOIUrl":"https://doi.org/10.1021/acschemneuro.4c00635https://doi.org/10.1021/acschemneuro.4c00635","url":null,"abstract":"Hibernating mammals such as the thirteen-lined ground squirrel (Ictidomys tridecemlineatus) experience significant reductions in oxidative metabolism and body temperature when entering a state known as torpor. Animals entering or exiting torpor do not experience permanent loss of brain function or other injuries, and the processes that enable such neuroprotection are not well understood. To gain insight into changes in protein function that occur in the dramatically different physiological states of hibernation, we performed quantitative phosphoproteomics experiments on thirteen-lined ground squirrels that are summer-active, winter-torpid, and spring-active. An important aspect of our approach was the use of focused microwave irradiation of the brain to sacrifice the animals and rapidly inactivate phosphatases and kinases to preserve the native phosphoproteome. Overall, our results showed pronounced changes in phosphorylated proteins for the transitions into and out of torpor, including proteins involved in gene expression, DNA maintenance and repair, cellular plasticity, and human disease. In contrast, the transition between the active states showed minimal changes. This study offers valuable insight into the global changes in brain phosphorylation in hibernating mammals, the results of which may be relevant to future therapeutic strategies for brain injury.","PeriodicalId":13,"journal":{"name":"ACS Chemical Neuroscience","volume":"16 3","pages":"428–438 428–438"},"PeriodicalIF":4.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serotonergic Mechanisms in Proteinoid-Based Protocells

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-22 DOI: 10.1021/acschemneuro.4c0080110.1021/acschemneuro.4c00801

Panagiotis Mougkogiannis*, and , Andrew Adamatzky,

引用次数: 0

Enhanced Analgesic Efficacy and Reduced Side Effects of Morphine by Combination with PD-1 Agonist

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-21 DOI: 10.1021/acschemneuro.4c0073210.1021/acschemneuro.4c00732

Xiaofei Song, Ying Zhang, Yuxin Liu, Gang Chen* and Long Zhao*,

{"title":"Enhanced Analgesic Efficacy and Reduced Side Effects of Morphine by Combination with PD-1 Agonist","authors":"Xiaofei Song, Ying Zhang, Yuxin Liu, Gang Chen* and Long Zhao*, ","doi":"10.1021/acschemneuro.4c0073210.1021/acschemneuro.4c00732","DOIUrl":"https://doi.org/10.1021/acschemneuro.4c00732https://doi.org/10.1021/acschemneuro.4c00732","url":null,"abstract":"Chronic pain is a debilitating disease and remains challenging to treat. Morphine serves as the most commonly used drug for the treatment of pathological pain. However, detrimental side effects (e.g., hyperalgesia and tolerance) manifest during chronic administration, thus counteracting morphine analgesia. Investigators have sought methods to widen the therapeutic window of morphine in the management of chronic pain. Programmed cell death protein 1 (PD-1) is a recently validated analgesic target and is coexpressed with the mu opioid receptor (μOR) in dorsal root ganglion (DRG) sensory neurons. Here, we present evidence that PD-1 regulates the expression of μOR mRNA and influences μOR-mediated analgesia. Notably, the concomitant administration of PD-1 agonist H-20 greatly reduces the dosage of morphine needed for analgesia, thereby significantly decreasing opioid-related side effects. This new combination therapy may provide a solution for managing chronic pain in patients who require morphine.","PeriodicalId":13,"journal":{"name":"ACS Chemical Neuroscience","volume":"16 3","pages":"490–499 490–499"},"PeriodicalIF":4.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143126834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking Neuroinflammation: A Balanced Art for Therapeutics of Prion Disease

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-21 DOI: 10.1021/acschemneuro.4c0087110.1021/acschemneuro.4c00871

Cao Chen, and , Xiaoping Dong*,

引用次数: 0

Hinokinin Decreases Methamphetamine-Induced Hyperlocomotion via the Regulatory Effects on Dopamine Levels

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-21 DOI: 10.1021/acschemneuro.4c0059210.1021/acschemneuro.4c00592

Byoung Mo Choi, Sun Mi Gu, Abdulaziz Jabborov, Min-Seok Yang, Sang Won Yeon, Chun-Woong Park*, Mi Kyeong Lee* and Jaesuk Yun*,

{"title":"Hinokinin Decreases Methamphetamine-Induced Hyperlocomotion via the Regulatory Effects on Dopamine Levels","authors":"Byoung Mo Choi, Sun Mi Gu, Abdulaziz Jabborov, Min-Seok Yang, Sang Won Yeon, Chun-Woong Park*, Mi Kyeong Lee* and Jaesuk Yun*, ","doi":"10.1021/acschemneuro.4c0059210.1021/acschemneuro.4c00592","DOIUrl":"https://doi.org/10.1021/acschemneuro.4c00592https://doi.org/10.1021/acschemneuro.4c00592","url":null,"abstract":"The global abuse of stimulant methamphetamine (METH) imposes a significant social burden. Despite this, effective therapeutic interventions for mitigating the harmful effects associated with METH-induced central nervous system (CNS) stimulation remain elusive. Chamaecyparis obtusa (hinoki), containing hinokinin as its active constituent, has been identified to exhibit CNS depressant properties. Here, we explored the potential of the hinoki extract and hinokinin in modulating METH-induced hyperlocomotion through the regulation of dopaminergic neuronal activity. We discovered that pretreatment with hinokinin significantly attenuates METH-induced locomotor activity, indicative of reduced CNS stimulation. Furthermore, treatment with hinokinin was observed to inhibit the METH-induced elevation in dopamine levels and the concomitant decrease in dopamine transporter (DAT) function within striatal brain slices of mice. In silico analysis coupled with pull-down assays and the dose-response curve substantiated the direct binding of hinokinin to DAT. We propose that hinokinin mitigates METH-induced hyperlocomotion via the inhibition of dopaminergic neurotransmission, with allosteric modulation of DAT playing a critical role in this regulatory mechanism. Collectively, our research suggests the potential of hinokinin to mitigate dopamine-mediated central nervous system excitation.","PeriodicalId":13,"journal":{"name":"ACS Chemical Neuroscience","volume":"16 3","pages":"393–404 393–404"},"PeriodicalIF":4.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, Pharmacological Characterization, and Binding Mode Analysis of 8-Hydroxy-Tetrahydroisoquinolines as 5-HT7 Receptor Inverse Agonists

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-21 DOI: 10.1021/acschemneuro.4c0066710.1021/acschemneuro.4c00667

Camilla B. Chan, Eline Pottie, Icaro A. Simon, Adrian G. Rossebø, Matthias M. Herth, Kasper Harpsøe, Jesper L. Kristensen, Christophe P. Stove* and Christian B. M. Poulie*,

{"title":"Synthesis, Pharmacological Characterization, and Binding Mode Analysis of 8-Hydroxy-Tetrahydroisoquinolines as 5-HT7 Receptor Inverse Agonists","authors":"Camilla B. Chan, Eline Pottie, Icaro A. Simon, Adrian G. Rossebø, Matthias M. Herth, Kasper Harpsøe, Jesper L. Kristensen, Christophe P. Stove* and Christian B. M. Poulie*, ","doi":"10.1021/acschemneuro.4c0066710.1021/acschemneuro.4c00667","DOIUrl":"https://doi.org/10.1021/acschemneuro.4c00667https://doi.org/10.1021/acschemneuro.4c00667","url":null,"abstract":"The serotonin 7 receptor (5-HT7R) regulates various processes in the central nervous system, including mood, learning, and circadian rhythm control, among others. Receptor activation can lead to activation of the Gαs protein and a subsequent increase of intracellular cyclic adenosine monophosphate (cAMP). Receptor interaction with inverse agonists results in a decrease of basal cAMP levels and therefore a downstream effect of reduced neuronal excitability and neurotransmission. Recently, pellotine (1a), a Lophophora alkaloid, was unexpectedly shown to be an inverse agonist of the 5-HT7R. Therefore, we evaluated close analogs of compound 1a, both naturally occurring and synthetic analogs, as inverse agonists of the 5-HT7R. Functional evaluation in a GloSensor cAMP assay revealed a preference for an 8-hydroxy-6,7-dimethoxy substitution pattern over 6,7,8-trimethoxy analogs or 8-hydroxy-6,7-methylenedioxy analogs. This was supported by molecular dynamics simulations, where the 8-hydroxy substitution allowed more robust interaction with the 5-HT7R, which correlated with inverse agonism efficacy. Additionally, N-methylation (as in 1a) improved the potency of the evaluated analogs. In this series, the most potent inverse agonist was anhalidine (1b) (EC50 = 219 nM, Emax = −95.4%), which lacks the 1-methyl, compared to pellotine (1a), and showed a 2-fold higher functional potency. Altogether, these results provide key insights for the further development of potent low molecular weight inverse agonists of the 5-HT7R.","PeriodicalId":13,"journal":{"name":"ACS Chemical Neuroscience","volume":"16 3","pages":"439–451 439–451"},"PeriodicalIF":4.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted Metabolomics for the Analysis of p-Cresol in Mouse Brain: Impact of Biological Sex and Strain

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-19 DOI: 10.1021/acschemneuro.4c0069810.1021/acschemneuro.4c00698

Laura Bertarini, Federico Imbeni, Antonietta Vilella, Silvia Alboni* and Federica Pellati*,

{"title":"Targeted Metabolomics for the Analysis of p-Cresol in Mouse Brain: Impact of Biological Sex and Strain","authors":"Laura Bertarini, Federico Imbeni, Antonietta Vilella, Silvia Alboni* and Federica Pellati*, ","doi":"10.1021/acschemneuro.4c0069810.1021/acschemneuro.4c00698","DOIUrl":"https://doi.org/10.1021/acschemneuro.4c00698https://doi.org/10.1021/acschemneuro.4c00698","url":null,"abstract":"p-Cresol, an environmental contaminant and endogenous metabolite derived primarily from the conversion of l-tyrosine by intestinal microflora, is gaining increasing attention, due to its potential impact on human health. Recent studies have highlighted elevated levels of p-cresol and its metabolites, including p-cresyl sulfate and p-cresyl glucuronide, in various populations, suggesting a correlation with neurodevelopmental and neurodegenerative conditions. While the role of this compound as a uremic toxin is well established, its presence and concentration within the central nervous system (CNS) remain largely unexplored. To address this gap, an high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was optimized and validated for the first time in this work for the targeted metabolomics of p-cresol in brain tissues. This method enabled the quantification of this compound in different brain areas of adult male and female C57BL/6J mice and in the cortex of various mouse strains, including CD-1 and the idiopathic autism model BTBR T+Itpr3tf/J. Additionally, preliminary analyses of human cortex samples confirmed the presence of p-cresol, suggesting its relevance in human brain health. Moreover, metabolomic analyses have further explored the correlations between p-cresol and neurotransmitters, with a particular focus on dopaminergic and noradrenergic pathways. These findings pave the way for understanding the potential impact of p-cresol on neurochemical networks and its implications for neurodevelopmental and neurodegenerative disorders.","PeriodicalId":13,"journal":{"name":"ACS Chemical Neuroscience","volume":"16 3","pages":"452–461 452–461"},"PeriodicalIF":4.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Dual-Functional Half-Curcumin Analogues as a Fluorescent and PET Probe for β-Amyloid Imaging in the Alzheimer’s Disease APP/PS1 Model

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-17 DOI: 10.1021/acschemneuro.4c0053210.1021/acschemneuro.4c00532

Haijun Yang, Ke Li, Tingfang Wang, Yi Zou, Guanyu Xu, Can Zhou, Zeying Liang, Yingqiu Wang*, Jianguo Lin* and Jian Yang*,

{"title":"Novel Dual-Functional Half-Curcumin Analogues as a Fluorescent and PET Probe for β-Amyloid Imaging in the Alzheimer’s Disease APP/PS1 Model","authors":"Haijun Yang, Ke Li, Tingfang Wang, Yi Zou, Guanyu Xu, Can Zhou, Zeying Liang, Yingqiu Wang*, Jianguo Lin* and Jian Yang*, ","doi":"10.1021/acschemneuro.4c0053210.1021/acschemneuro.4c00532","DOIUrl":"https://doi.org/10.1021/acschemneuro.4c00532https://doi.org/10.1021/acschemneuro.4c00532","url":null,"abstract":"Noninvasive imaging of β-amyloid in vivo is pivotal for the early diagnosis of Alzheimer’s disease (AD). While single imaging methods have been extensively studied for detecting Aβ over the past decade, dual-modal probes have received scant attention. In this study, we synthesized and assessed a series of half-curcumin probes, among which DiFboron-8 demonstrated a high affinity and selectivity for Aβ aggregates. DiFboron-8 effectively served as a dual-functional fluorescent and positron emission tomography (PET) probe for imaging β-amyloid in an AD mouse model. Histological staining results underscored DiFboron-8’s potent staining capability for Aβ plaques in APP/PS1 brain slices, while ex vivo biodistribution studies highlighted its rapid clearance rate. In vivo imaging revealed that [18F]-DiFboron-8 could penetrate the blood–brain barrier, displaying higher PET signals in the brains of APP/PS1 mice compared to wild-type mice just 3 min postinjection, a finding corroborated by autoradiography staining. Overall, we propose that [18F]-DiFboron-8 represents an efficient fluorescent/PET dual-modal probe, offering promise for β-amyloid imaging in the early stages of AD.","PeriodicalId":13,"journal":{"name":"ACS Chemical Neuroscience","volume":"16 3","pages":"365–373 365–373"},"PeriodicalIF":4.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the Effects of Light, Darkness, and Dim Light on Rat Brain Tissue: A Biochemical and Histological Study

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-17 DOI: 10.1021/acschemneuro.4c0075710.1021/acschemneuro.4c00757

Hıdır Pekmez*, Tuğba Raika Kıran, Fahriye Seçil Tecelli̇oğlu, Feyza İnceoğlu, Merve Aydin, Emrah Zayman and Sinan Canpolat,

{"title":"Investigation of the Effects of Light, Darkness, and Dim Light on Rat Brain Tissue: A Biochemical and Histological Study","authors":"Hıdır Pekmez*, Tuğba Raika Kıran, Fahriye Seçil Tecelli̇oğlu, Feyza İnceoğlu, Merve Aydin, Emrah Zayman and Sinan Canpolat, ","doi":"10.1021/acschemneuro.4c0075710.1021/acschemneuro.4c00757","DOIUrl":"https://doi.org/10.1021/acschemneuro.4c00757https://doi.org/10.1021/acschemneuro.4c00757","url":null,"abstract":"This study evaluates acetylcholinesterase (AChE) enzyme activity levels, oxidative stress parameters, histopathological findings, and serum melatonin levels in rat brain tissue. 32 male Wistar Albino rats were randomly divided into four groups: Control, Light, Dark, Dim light (n = 8 each group). After a 30 day experiment, brain tissues were collected to measure AChE, glutathione S-transferase (GST), glutathione (GSH), and malondialdehyde (MDA) levels and conduct histopathological analyses. Serum melatonin levels were also measured. In this study, we observed a significant increase in MDA levels in dim light, dark, and light groups. AChE and α-GST enzyme activity levels were significantly decreased in the dark group compared with the other groups. Additionally, there was a statistical difference in melatonin levels between the light and dark groups. In the light microscope examination of the sections stained with hematoxylin-eosin from the dark group brain tissue, mild perineuronal edema was observed in all areas. Our study is the first to compare the effects of three groups on the brain: continuous light, continuous darkness, and dim light at night. Additionally, it is the only study to examine the effects of light exposure differences on the brain AChE levels.","PeriodicalId":13,"journal":{"name":"ACS Chemical Neuroscience","volume":"16 3","pages":"513–518 513–518"},"PeriodicalIF":4.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactivity of Olanzapine and Tricyclic Antidepressants on the Protective Effects of Trolox on Lipid Peroxidation Evaluated Using Fluorescence Anisotropy, Electron Paramagnetic Resonance Spectrometry, and Thermal Analysis

IF 4.1 3区医学

ACS Chemical Neuroscience Pub Date : 2025-01-17 DOI: 10.1021/acschemneuro.4c0070210.1021/acschemneuro.4c00702

Yusuke Horizumi, Reo Tanada, Yuya Kurosawa, Miwa Takatsuka, Tomohiro Tsuchida and Satoru Goto*,

{"title":"Reactivity of Olanzapine and Tricyclic Antidepressants on the Protective Effects of Trolox on Lipid Peroxidation Evaluated Using Fluorescence Anisotropy, Electron Paramagnetic Resonance Spectrometry, and Thermal Analysis","authors":"Yusuke Horizumi, Reo Tanada, Yuya Kurosawa, Miwa Takatsuka, Tomohiro Tsuchida and Satoru Goto*, ","doi":"10.1021/acschemneuro.4c0070210.1021/acschemneuro.4c00702","DOIUrl":"https://doi.org/10.1021/acschemneuro.4c00702https://doi.org/10.1021/acschemneuro.4c00702","url":null,"abstract":"Multiacting receptor-targeting antipsychotics and tricyclic antidepressants stimulate various neurotransmitter receptors despite the different targets of postsynaptic receptors and presynaptic reuptake transporters. Their auxiliary and adverse effects may be caused by multiple targets or the modification of the neuronal membrane. To evaluate the membrane responses to olanzapine, imipramine, desipramine, amitriptyline, lidocaine, and dibucaine, we examined the inhibition of lipid peroxidation in egg yolk phosphatidylcholine liposomes. By contrast, their effects on membrane fluidity were measured as the suppressive contributions of the inhibitory activity of Trolox on lipid oxidation. These drugs inhibit lipid peroxidation and exclude harmful reactive oxygen species and the protective effect of Trolox. The fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene in saturated phospholipid liposome-containing drugs suggested that olanzapine, imipramine, and dibucaine enhanced membrane fluidity. The radical scavenging activity of 2,2-diphenylpicrylhidrazyl and galvinoxyl radicals was determined using electron paramagnetic resonance experiments, and their molecular flexibility was determined using thermograms for differential scanning calorimetry. Multiple regression analyses of the linear free energy relationship approach and comparative investigations revealed that the membranous fluidity of the liposomes, independent of the radical scavenging activity of the drugs, induced the inhibitory activity on lipid peroxidation. We discussed how these drugs act on nervous membranes and aimed to identify the relationship between uncertified functions and membranous fluidity.","PeriodicalId":13,"journal":{"name":"ACS Chemical Neuroscience","volume":"16 3","pages":"462–478 462–478"},"PeriodicalIF":4.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acschemneuro.4c00702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0