International Journal of Biochemistry Research and Review最新文献_第6页

Effect of Boiling on Chemicals, Phytochemicals and Nutritional Composition of Solanum nigrum L. Leaves Harvested in Abidjan (Côte d’Ivoire) 煮沸对阿比让(Côte d 'Ivoire)采收茄叶化学物质、植物化学物质和营养成分的影响

International Journal of Biochemistry Research and Review Pub Date : 2020-02-01 DOI: 10.9734/ijbcrr/2019/v28i430159

Dogni Kouassi Dappah, Denis Yao N’Dri, Nestor Kouakou Kouassic, G. Amani

{"title":"Effect of Boiling on Chemicals, Phytochemicals and Nutritional Composition of Solanum nigrum L. Leaves Harvested in Abidjan (Côte d’Ivoire)","authors":"Dogni Kouassi Dappah, Denis Yao N’Dri, Nestor Kouakou Kouassic, G. Amani","doi":"10.9734/ijbcrr/2019/v28i430159","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i430159","url":null,"abstract":"Aims: In tropical Africa, leafy vegetables are traditionally cooked and eaten as a relish together with a starchy staple food. The current study aimed to evaluate the influence of boiling on bioactive, proximate and antinutrients compounds in Solanum nigrum leaves. Methodology: The leaves were subjected to boiling in pressure cooker for 10, 15 and 20 min and proximate composition, minerals, nutritive and anti-nutritional components were determined according to standard methods for nutritional guidelines. Results: The result of the study revealed that longer time of boiling (higher than 10 min) caused negative impact by reducing nutritive value but positive impact by reducing anti-nutrients. The Original Research Article Dappah et al.; IJBCRR, 28(4): 1-11, 2019; Article no.IJBCRR.52752 2 registered losses (p < .05) at 10 min were as follows: ash (12.37%), proteins (33.69%), vitamin C (11.23 mg/ 100 g), polyphenols (125.41 mg/ 100 g) Tannins (81.96 mg/ 100 g) and Flavonoids (14.03 mg/100 g). The average increase of fibers content was (39.41%). Furthermore, after 10 min of boiling time the residual contents (p<0.05) of minerals was: Calcium (1079±29.04 to 386±78 mg/ 100 g), Phosphorus (405..24±10-508.88±2.58 mg/ 100 g), Iron (33.48±0.93–44.50±1.23 mg / 100 g) and Magnesium (572.78±19.76–662.99±27.23 mg/ 100 g).The antinutrient composition for oxalic acid were ranged 72.61±6.29 to 223.67±6.35 mg/100 g. The different ratios sodium / potassium (<1) and oxalates/calcium (<2.5) were under the respective critical value for all boiling. Conclusion: These results suggest that the recommended time of domestic cooking must be less than 10 min for the studied leafy vegetables in order to contribute efficiently to the nutritional requirement and to the food security of Ivorian population.","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"12 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78512937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Proximate, Phytochemicals and Reducing Power of Leaf Extracts of Colocasia esculenta and Ipomoea batatas 土芋叶提取物的近因、植物化学成分及还原性

International Journal of Biochemistry Research and Review Pub Date : 2020-01-31 DOI: 10.9734/ijbcrr/2019/v28i430158

Achadu Abraham Eleojo, Umeh Chisom Charles, M. Nimat

{"title":"Proximate, Phytochemicals and Reducing Power of Leaf Extracts of Colocasia esculenta and Ipomoea batatas","authors":"Achadu Abraham Eleojo, Umeh Chisom Charles, M. Nimat","doi":"10.9734/ijbcrr/2019/v28i430158","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i430158","url":null,"abstract":"Background: Reports has shown that edible leaves of vegetable plants serve both nutritional and medicinal purposes, yet are poorly utilized due to inadequate enlightenment of the major populace on their compositions. Aims: The aim of this study was to determine the proximate, phytochemicals and reducing power of leaf extracts of Colocasia esculenta and Ipomoea batatas. Methodology: Washed and air dried leaf samples were milled and subjected to proximate and phytochemical analysis. Determination of calcium, iron and sodium content was by Atomic absorption spectroscopy. Reducing power was determined by the Potassium ferricyanide reducing power method. Results: Ipomoea batatas leaves was found to be higher in moisture (14.05%) and carbohydrate (29.33%) while Colocasia esculenta leaves was higher in ash (10.00%), Crude fiber (16.27%), Fat Original Research Article Achadu et al.; IJBCRR, 28(4): 1-11, 2019; Article no.IJBCRR.52703 2 (10.17%) and protein (29.41%). A better antioxidant activity and higher levels of all phytochemicals and minerals were observed in leaves of Colocasia esculenta compared to leaves of Ipomoea batatas. Conclusion: This study suggests that both leaves are of importance to human nutrition considering the observed levels of nutrients, phytochemicals and antioxidant activity. While C. esculenta leaves should be preferred for its nutrient and antioxidant advantages, both leaves can contribute immensely to the daily nutrient requirements.","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"1 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2020-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89825423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Enzyme Functional Screening, Discovery and Engineering; Automation, Metagenomics and High-throughput Approaches 酶的功能筛选、发现与工程自动化、宏基因组学和高通量方法

International Journal of Biochemistry Research and Review Pub Date : 2020-01-20 DOI: 10.9734/ijbcrr/2019/v28i430156

Sikander Ali, Syed muhammad Shahid Hussain

{"title":"Enzyme Functional Screening, Discovery and Engineering; Automation, Metagenomics and High-throughput Approaches","authors":"Sikander Ali, Syed muhammad Shahid Hussain","doi":"10.9734/ijbcrr/2019/v28i430156","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i430156","url":null,"abstract":"Concerned with the construction and design of novel biocatalysts, the enzyme engineering served to overcome the limitations of native enzymes, in order to create biocatalysts with tailored functions, to facilitate industrial applications. The enzymes, being recognized by screening and discovery workflows and further tailored by engineering platforms, are of immense potential as improved biocatalysts. Functional metagenomics is a powerful tool to identify novel enzymes followed by the construction of metagenome-based enzyme libraries. And the subsequent screening of these enzyme libraries is in turn facilitated by ultra-high-throughput-based, for example FACS or microfluidics, enzyme engineering technologies. Relies on the compartmentalization of reaction components, in order to detect and measure assay signal within the reaction compartments, the enzyme engineering platforms are designed which include cell-as-compartment platforms, dropletbased platforms and micro-chamber-based platforms. The metagenomics approach and highthroughput screening by these three prime enzyme engineer platforms are the focus of this review. Review Article","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"14 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2020-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79166507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Fractional Composition of Dietary Supplements "Majmui Rahmoniy" on Mycobacterium smegmatis 膳食补充剂“Majmui rahmony”部分成分对耻垢分枝杆菌的影响

International Journal of Biochemistry Research and Review Pub Date : 2020-01-04 DOI: 10.9734/ijbcrr/2019/v28i430155

I. Mamatova, M. Mamarakhmonov, I. Askarov

引用次数: 0

Effect of Oral Administration of Diclofenac Sodium on Coagulation Factors and Some Hematological Parameters in Wister Rats 口服双氯芬酸钠对Wister大鼠凝血因子及部分血液学指标的影响

International Journal of Biochemistry Research and Review Pub Date : 2019-12-28 DOI: 10.9734/ijbcrr/2019/v28i430154

Abraham Zorte, Jonathan Esima, Waribo Helen, D. Onwuli

{"title":"Effect of Oral Administration of Diclofenac Sodium on Coagulation Factors and Some Hematological Parameters in Wister Rats","authors":"Abraham Zorte, Jonathan Esima, Waribo Helen, D. Onwuli","doi":"10.9734/ijbcrr/2019/v28i430154","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i430154","url":null,"abstract":"Diclofenac sodium is a nonsteroidal anti-inflammatory drug often obtainable as a prescription drug or over the counter. It is very effective in the control of inflammation and pain due to arthritis or pains arising following many disease conditions because of its antipyretic, anti inflammatory and analgesic potentials. Despite the beneficial effects of diclofenac sodium, it has been implicated in some adverse effects. In this study, we examined the effect of acute and chronic administration of diclofenac sodium on some hematological (PCV, WBC differentials) and coagulation (prothrombin time, activated partial prothrombin time and platelets count) parameters of albino Wister rats using the standard methods. Twenty four Albino Wister rats were divided into three groups of 8 rats and grouped as control, acute study and chronic study. The rats were administered 0.2 mg of diclofenac sodium for 24 hours for acute and 3 weeks for chronic studies respectively. The rats were sacrificed and blood collected for analysis of PCV, WBC differentials, prothrombin time, activated partial prothrombin time and platelets count using the standard methods. Results show that acute administration of diclofenac sodium at 0.2 mg has no effect on hematological and coagulation parameters, but chronic administration could instigate significant reduction in PCV, platelets count, neutrophils and monocytes (p<0.001), while there is a significant increase in PT, INR, lymphocytes (p<0.001). Considering these alterations, it is advisable that this drug should be made a strictly prescription drug in order to prevent indiscriminate use of this medication and to prevent attendant anemia and coagulopathy that may follow chronic use.","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"24 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87284075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitors of Cellulase Activities According to the Trophic Group of Termites (Insecta: Isoptera) from Daloa (Côte d'Ivoire) Daloa (Côte d'Ivoire)白蚁营养类群纤维素酶活性抑制剂的研究

International Journal of Biochemistry Research and Review Pub Date : 2019-12-24 DOI: 10.9734/ijbcrr/2019/v28i430153

Blei Sika Hortense, T. C. Sylvain, Eba Krou Philippe, B. Maxwell, Faulet Meuwiah Betty, N. Sébastien, K. Patrice

{"title":"Inhibitors of Cellulase Activities According to the Trophic Group of Termites (Insecta: Isoptera) from Daloa (Côte d'Ivoire)","authors":"Blei Sika Hortense, T. C. Sylvain, Eba Krou Philippe, B. Maxwell, Faulet Meuwiah Betty, N. Sébastien, K. Patrice","doi":"10.9734/ijbcrr/2019/v28i430153","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i430153","url":null,"abstract":"The presence of termites in the cocoa plantations and quarries of Côte d'Ivoire poses a threat to the producers of this sector. Producer yields are insufficient to cover the strong market demand. This situation leads to food insecurity for the population. Knowledge of the specific inhibitory molecules of digestive enzymes of termites is necessary to enhance the effectiveness of insecticides to optimize crop production. The present study was aimed to characterize termite cellulases according to the trophic group. Specifically, the influence of chemical agents on the Original Research Article Hortense et al.; IJBCRR, 28(4): 1-7, 2019; Article no.IJBCRR.53462 2 cellulase activities of four humivorous (Cubitermes fungifaber) and xylophagous termites (Nasutitermes latifrons, Microcerotermes fuscotibialis and Amitermes guineensis) collected in Daloa during the October period was investigated. Thus, the cellulase activities were measured by the spectrophotometric method in the absence and in the presence of the concentrations of 1 and 5 mM of various chemical agents. The chemical agents used behaved differently on cellulase activities. Thus, Cu, Pb and EDTA inhibited the cellulase activity of M. fuscotibialis more than 90% at concentrations of 1 and 5 mM, respectively, indicating the presence of a metalloprotein. On the other hand, that of the other two xylophagous species was slightly inhibited. In addition, the cellulase activity of C. Fungifaber was inhibited at the two respective concentrations by Cu 2+ at about 70%. In conclusion, Cu 2+ , pb 2+ and EDTA can be used in the formulation of some specific insecticides against humivorous and xylophagous termites.","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"29 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2019-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87072001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Chelation Therapy and Blood Transfusion on Trace Elements in Thalassemia Patients 螯合治疗及输血对地中海贫血患者微量元素的影响

International Journal of Biochemistry Research and Review Pub Date : 2019-12-18 DOI: 10.9734/ijbcrr/2019/v28i430152

Vijaysinh Parmar, Kuldip Singh, Asha Khubchandani

{"title":"Effect of Chelation Therapy and Blood Transfusion on Trace Elements in Thalassemia Patients","authors":"Vijaysinh Parmar, Kuldip Singh, Asha Khubchandani","doi":"10.9734/ijbcrr/2019/v28i430152","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i430152","url":null,"abstract":"Background: β-Thalassemia major is considered to be one of the most common inherited hemolytic anemia. Enhanced years of survival of thalassemia have led to unmasking related complications related to alterations in certain trace elements like magnesium, calcium, phosphorus, copper, zinc etc. Objective: Present study was conducted to evaluate the effect of iron chelation therapy and blood transfusion on certain trace elements (Magnesium, Calcium, Phosphorus, Copper, Zinc) in βthalassemic patients on chelation therapy more than one year. Materials and Methods: In the present cross sectional study, 100 β-thalassemic patients receiving chelation therapy for atleast 1year were recruited from Civil Hospital Ahmedabad, Gujarat during February, 2017 to December, 2018 and equal number (n=100) of healthy subjects were taken as a control group in the age range of 8 to 15 years of both sexes (male & female). The levels of serum magnesium, calcium, phosphorus, zinc, and copper in serum were analyzed and results were correlated with normal healthy subjects. Original Research Article Parmar et al.; IJBCRR, 28(4): 1-5, 2019; Article no.IJBCRR.53338 2 Results: A significant increase in serum copper (P≤ 0.01) and phosphorus (P≤ 0.001) were observed levels while a significant (P≤ 0.05) fall in magnesium, calcium and zinc levels recorded in β-thalassemic patients in comparison to healthy control subjects. Conclusion: Aforementioned observations suggested that fluctuations in the trace elements levels in βthalassemic children receiving blood transfusion and iron chelation therapy could leads to different complications like hemolyzed red cells, infections & hemochromatosis renal damage, hypoparathyroidism etc. if remains untreated. Hence routine assessment of these elements is recommended for better management.","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"99 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2019-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87238747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nephrotoxicological Effect of Water Soluble Fraction of Bonny Light Crude Oil in Wistar Albino Rats 邦尼轻质原油水溶性组分对Wistar白化大鼠肾毒性的影响

International Journal of Biochemistry Research and Review Pub Date : 2019-12-14 DOI: 10.9734/ijbcrr/2019/v28i430151

Chiedozie Nicholas Abraham, G. Udom, K. Patrick-Iwuanyanwu

{"title":"Nephrotoxicological Effect of Water Soluble Fraction of Bonny Light Crude Oil in Wistar Albino Rats","authors":"Chiedozie Nicholas Abraham, G. Udom, K. Patrick-Iwuanyanwu","doi":"10.9734/ijbcrr/2019/v28i430151","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i430151","url":null,"abstract":"This study evaluated the Nephrotoxic effect of water soluble fraction (WSF) of Bonny Light Crude Oil (BLCO). After preparation of the WSF and a range finding test, the Wistar albino rats were administered three concentrations (25%, 50% and 100%) of WSF of BLCO for 30 and 60days. Data from the study showed that Urea concentration increased significantly (p≤0.05) with increasing dose of BLCO ranging from 14.71 mg/dl in the control to 35.28 mg/dl in the 100% group after 30days and 14.28 mg/dl in the control to 41.08mg/dl in the 100% group after 60days, Creatinine concentration increased significantly (p≤0.05) from 0.22 mg/dl in the control to 0.82mg/dl in the 100% group after 60 days administration while electrolyte (Na, K, Cl) concentration increased significantly (p≤0.05) with increasing dose of BLCO after 60days administration. Histopathological examination of the kidney was characterized by partial partitioning of the glomerular tufts, obliteration of the Bowman’s capsule and distortion of the renal tubules. The findings in this research suggest that WSF of BLCO induced nephrotoxicity.","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"8 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2019-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84593087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Storage on Nutritional, Mineral Composition and Mycoflora of Stored Sundried Citrullus lanatus Thunberg (Melon) Seeds 贮藏对贮藏晒瓜种子营养、矿物组成及菌群的影响

International Journal of Biochemistry Research and Review Pub Date : 2019-12-13 DOI: 10.9734/ijbcrr/2019/v28i430149

E. Fagbohun, A. Ogundahunsi

{"title":"Effects of Storage on Nutritional, Mineral Composition and Mycoflora of Stored Sundried Citrullus lanatus Thunberg (Melon) Seeds","authors":"E. Fagbohun, A. Ogundahunsi","doi":"10.9734/ijbcrr/2019/v28i430149","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i430149","url":null,"abstract":"The effect of storage on the nutritive values, proximate composition and mycoflora of Citrullus lanatus was investigated during a 24 weeks of storage. The mycoflora were isolated using direct plating and washing methods. Eight fungi were isolated namely Fusarium sp., Rhizopus sp., Mucor sp., Aspergillus niger, A. fumigatus, A. flavus, Penicillium chrysogenum and Penicillium sp. The fungal count was found to increase as the storage time increased. The proximate analysis showed a decrease and an increase on some parameters investigated. The mineral composition was found to decrease as the storage time increased. The following nutrients (g/100 g) were found to have reduced: ash content 4.29 3.19, fibre content 2.06 1.51, fat content 57.02 50.90, while the following increased: Moisture content 7.21-12.22, crude protein 29.24-30.90, carbohydrate content 0.20-2.01. The results for minerals analysis (mg/100 g) showed a decrease in all parameters investigated: sodium 2.01-1.49, potassium 65.52-56.21, calcium 13.10-10.19, magnesium 46.34Original Research Article Fagbohun and Ogundahunsi; IJBCRR, 28(4): 1-7, 2019; Article no.IJBCRR.51860 2 37.61, Iron 8.12-3.31, zinc 4.76-1.24, copper 2.84-2.11 and phosphorus 3.46-1.60 while lead and cadmium were not detected. However, storage was found to cause deterioration and reduction in the nutritive value and mineral content of C. lanatus seeds. It is therefore recommended that these seeds must be stored under controlled conditions that will prevent the growth of fungi and reduction of nutrients.","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"36 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2019-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78764503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Evaluating the Effect of Iron (II) Chloride Induced Oxidative Stress in SH-SY5Y Cells and Its Role in Upregulation of α-Synuclein Expression 氯化铁诱导SH-SY5Y细胞氧化应激的作用及其在α-突触核蛋白表达上调中的作用

International Journal of Biochemistry Research and Review Pub Date : 2019-12-12 DOI: 10.9734/ijbcrr/2019/v28i330148

Mohammed Abba Dige, Emmanuel Paul Okoi

{"title":"Evaluating the Effect of Iron (II) Chloride Induced Oxidative Stress in SH-SY5Y Cells and Its Role in Upregulation of α-Synuclein Expression","authors":"Mohammed Abba Dige, Emmanuel Paul Okoi","doi":"10.9734/ijbcrr/2019/v28i330148","DOIUrl":"https://doi.org/10.9734/ijbcrr/2019/v28i330148","url":null,"abstract":"The α-synuclein (SNCA) gene is a pathogenic gene identified in rare familial Parkinson Disease (PD). Recent studies highlight the role of DNA methylation in the pathogenesis of familial and sporadic PD. Hypomethylation in SNCA gene has been associated with increased SNCA gene expression and was observed in post mortem brains of patients with sporadic PD. This study was aimed at evaluating the effect of iron (II) chloride on SH-SY5Y cell models as pertain to cell death caused by oxidative stress, upregulation of SNCA gene expression and reduced SNCA gene methylation. Result obtained from LDH assay showed significant (p < 0.05) evidence of cell death in treated cells as compared to the control sample. Analysis for SNCA gene quantification using RT-PCR showed significant increases in fold change. Cells treated with 1000μM of FeCl2 showed the highest fold change of 6.0 while cells treated with 250μM had the lowest fold change of 1.8. In DNA methylation assay using pyrosequencing, cells treated with varying concentrations of FeCl2 Original Research Article Dige and Okoi; IJBCRR, 28(3): 1-10, 2019; Article no.IJBCRR.53099 2 showed significant (p < 0.05) decrease in DNA methylation. At 250μM, 500μM and 750μM concentrations of FeCl2, an average mean methylation levels of 1.84%, 1.40% and 1.23% was obtained respectively while cells treated with 1000 μM had the lowest average mean methylation level of 1.0%. Thus, the decrease in methylation is linked to the upregulation of the SNCA gene which has been reported to be among the causative factors in the pathogenesis of Parkinson’s disease.","PeriodicalId":13942,"journal":{"name":"International Journal of Biochemistry Research and Review","volume":"3 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89279558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0