International Journal of Analytical Chemistry最新文献

{"title":"Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics.","authors":"Fan Chen,&nbsp;Yizhe Ma,&nbsp;Ying Cui,&nbsp;Wanhang Wang,&nbsp;Chenchen Mei,&nbsp;Jingjing Nie,&nbsp;Congcong Wen,&nbsp;Xiuwei Shen,&nbsp;Xuzhao Zhou","doi":"10.1155/2023/4747771","DOIUrl":"10.1155/2023/4747771","url":null,"abstract":"<p><p>An ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of tenacissoside G, tenacissoside H, and tenacissoside I in rat plasma. The rat plasma was treated with liquid-liquid extraction using ethyl acetate. The determination was performed on the UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 <i>μ</i>m) with a mobile phase consisting of acetonitrile-water (containing 0.1% formic acid) and gradient elution at a flow rate of 0.4 mL/min. Electrospray (ESI) positive ion mode detection and multireaction monitoring (MRM) quantitative analysis were performed. A total of 36 rats were given tenacissoside G, tenacissoside H, and tenacissoside I, respectively, orally (5 mg/kg) and intravenously (1 mg/kg), with 6 rats in each group, to evaluate the pharmacokinetic difference of tenacissoside G, tenacissoside H, and tenacissoside I in rats. The calibration curves showed good linearity in the range of 5-2000 ng/mL, where <i>r</i> was greater than 0.99. The results of precision, accuracy, recovery, matrix effect, and stability met the requirements of biological sample detection methods. The established UPLC-MS/MS method was successfully applied to pharmacokinetic studies of tenacissoside G, tenacissoside H, and tenacissoside I, and the bioavailability was 22.9%, 89.8%, and 9.4%, respectively.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"4747771"},"PeriodicalIF":1.8,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10555494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41127822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild Mushrooms: A Hidden Treasure of Novel Bioactive Compounds.","authors":"Gebreselema Gebreyohannes,&nbsp;Desta Berhe Sbhatu","doi":"10.1155/2023/6694961","DOIUrl":"https://doi.org/10.1155/2023/6694961","url":null,"abstract":"<p><p>Secondary metabolites are hidden gems in mushrooms. Understanding these secondary metabolites' biological and pharmacological effects can be aided by identifying them. The purpose of this work was to profile the mycochemical components of the extracts of <i>Auricularia auricula judae</i>, <i>Microporus xanthopus</i>, <i>Termitomyces umkowaani</i>, <i>Trametes elegans</i>, and <i>Trametes versicolor</i> to comprehend their biological and pharmacological capabilities. Mushroom samples were collected from Kenya's Arabuko-Sokoke and Kakamega National Reserved Forests and identified using morphological and molecular techniques. Chloroform, 70% ethanol, and hot water solvents were used to extract the mycochemical components. Gas chromatography mass spectrometry (GC-MS) was used to analyze the chloroform, 70% ethanol, and hot water extracts of all the species examined. A total of 51 compounds were isolated from all extracts and classified as carboxylic acids, esters, phenols, fatty acids, alcohol, epoxides, aldehydes, fatty aldehydes, isoprenoid lipids, and steroids. Tetracosamethyl-cyclododecasiloxane (18.90%), oleic acid (72.90%), phenol, 2, 6-bis (1, 1-dimethylethyl)-4-methyl-, and methylcarbamate (26.56%) were all found in high concentrations in <i>A. auricular judae</i>, <i>M. xanthopus</i>, <i>T. umkowaani</i>, <i>T. elegans</i>, and <i>T. versicolor</i>, respectively. Fatty acids make up the majority of the compounds isolated from the <i>T. elegans</i> chloroform extract and the <i>T. umkowaani</i> 70% ethanol extract, respectively. Particularly, these fatty acids play crucial roles in the anti-inflammatory, hypocholesterolemic, anticancer, and antibiofilm formation activities. These bioactive elements indicate that the extracts of five wild mushrooms may be reliable sources of secondary metabolites for therapeutic development. Therefore, additional research is required to comprehend the usefulness of these chemicals in many functional areas and to improve the present understanding of macrofungi.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"6694961"},"PeriodicalIF":1.8,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41114043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Prediction of Dissolved Oxygen Concentration in Sewage Treatment Process Based on Data Recognition Algorithm.","authors":"International Journal Of Analytical Chemistry","doi":"10.1155/2023/9807109","DOIUrl":"10.1155/2023/9807109","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/1525902.].</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"9807109"},"PeriodicalIF":1.8,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41119749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Methanol, Acetaldehyde, and Ethyl Acetate in Thousand Folds of Ethanol Sample by Headspace Gas Chromatography with Mass Spectrometry.","authors":"Nguyen Quoc Thang,&nbsp;Le Van Tan","doi":"10.1155/2023/8851265","DOIUrl":"https://doi.org/10.1155/2023/8851265","url":null,"abstract":"<p><p>Alcohol beverages have been widely consumed in several parts of the world. In this study, volatile organic compounds in alcoholic beverages including acetaldehyde, ethyl acetate, methanol, and higher ethanol were investigated and evaluated using a headspace gas chromatograph equipped with a mass spectrometer. This study evaluated the suitability of the chromatographic system, linearity, limit of detection, and limit of quantification, accuracy, and precision of the single and simultaneous determination of acetaldehyde, ethyl acetate, and methanol in thousand folds of ethanol. Results showed that the acetaldehyde concentration in local beer samples and local manual product liqueur samples ranged from 4.65 to 13.22 mg/L and from 5.55 to 75.96 mg/L, respectively, but in local industrial product liqueur samples, acetaldehyde was not detected. Methanol was only detected in a few local beer samples and locally manually produced liqueur samples within low concentrations. Ethyl acetate was only detected in all local beer samples, but it was not present in local industrial product liqueur samples.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"8851265"},"PeriodicalIF":1.8,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41110611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification.","authors":"Saiwei Wu,&nbsp;Abdu Ahmed Abdullah Al-Maskri,&nbsp;Qun Li,&nbsp;Jiatong Liu,&nbsp;Sheng Cai","doi":"10.1155/2023/6624884","DOIUrl":"https://doi.org/10.1155/2023/6624884","url":null,"abstract":"<p><p>A novel ligation-based loop-mediated isothermal amplification has been developed for miRNA detection. Two stem-loop structure DNA linker A/B probes which hybridized with miRNA were designed to establish a rapid and ultrasensitive miRNA-LAMP system for miRNA detection. Target miR-200a was used to template the ligation of Linker A/B probes with SplintR Ligase and used as a dumbbell-shaped amplicon. By adding BIP/FIP and Bst 2.0 DNA polymerase, the LAMP reaction was carried out, which brought greatly improved amplification efficiency. The double-stranded DNA fluorescent dye EvaGreen was added for the detection of amplification product to achieve the quantification of the target miRNA. This method can detect miRNA in a linear range of seven orders of magnitude, with a detection limit of 100 fM. Therefore, this ultrasensitive miRNA-LAMP assay provides a new path for the highly sensitive quantitative analysis of miRNA, thereby bringing convenience to clinical diagnosis and prognostic research.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"6624884"},"PeriodicalIF":1.8,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41157738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Antibiotics Residues in Milk and Meat Using Different Analytical Methods.","authors":"Melaku Getahun, Rahel Belete Abebe, Ashenafi Kibret Sendekie, Alem Endeshaw Woldeyohanis, Asmamaw Emagn Kasahun","doi":"10.1155/2023/4380261","DOIUrl":"10.1155/2023/4380261","url":null,"abstract":"<p><p>Veterinary drugs are pharmacologically and biologically active chemical agents. At present, veterinary drugs are extensively used to prevent and treat animal diseases, to promote animal growth, and to improve the conversion rate of feed. However, the use of veterinary drugs in food-producing animals may leave residues of the parent compounds and/or their metabolites in food products resulting in harmful effects on humans. To ensure food safety, sensitive and effective analytical methods have been developing rapidly. This review describes sample extraction and cleanup methods, and different analytical techniques are used for the determination of veterinary drug residues in milk and meat. Sample extraction methods, such as solvent extraction, liquid-liquid extraction, and cleanup methods such as dispersive solid-phase extraction and immunoaffinity chromatography, were summarized. Different types of analytical methods such as microbial, immunological, biosensor, thin layer chromatography, high-performance liquid chromatography, and liquid chromatography-tandem mass spectrometry were discussed for the analysis of veterinary drug residues in animal-derived foods. Liquid chromatography-tandem mass spectrometry is the most widely used analytical technique for the determination of antibiotic drug residues. This is due to the powerful separation of LC and accurate identification of MS, and LC-MS/MS is more popular in the analysis of veterinary drug residues.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"4380261"},"PeriodicalIF":1.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9809577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing Anthocleista nobilis","authors":"Rahinatu Ali Bawa, John Nii Addotey, Abena Amponsaa Brobbey, James Oppong-Kyekyeku, Christopher Kodjo Hlordzi","doi":"10.1155/2023/6957519","DOIUrl":"https://doi.org/10.1155/2023/6957519","url":null,"abstract":"<i>Anthocleista nobilis</i> is a common constituent in numerous conventional medications in West Africa. The stem bark of <i>A. nobilis</i> is known to contain brucine and is used to treat intestinal parasites, stomachaches, gonorrhoea, wounds, etc. The extensive use has led to high market value and adulteration of their herbal products. In this work, the antioxidant properties of extracts of <i>A. nobilis</i> are verified, and a validated RP-HPLC method is used to estimate the brucine content of extracts and products containing <i>A. nobilis</i>. Stem bark extracts from ethyl acetate, 96% v/v ethanol, 70% v/v ethanol, and water of <i>A. nobilis</i> were investigated for phytochemical content using standard methods and their antioxidant activity using DPPH and phosphomolybdenum assays. An RP-HPLC method was developed and validated using brucine as a reference standard. The optimized conditions of the developed RP-HPLC method include a Supelcosil C18 column with dimensions 150 × 4.6 mm and particle size of 3 <i>μ</i>m, a mobile phase comprising of MeOH : 0.1% v/v HCOOH (65:35 %v/v ) which was injected at a flow rate of 0.8 mL/min, and an injection volume of 50 <i>μ</i>L. The wavelength of detection was 274 nm. The developed method was validated as per ICH guidelines. The common phytochemicals among the various extracts were tannins and alkaloids. All extracts exhibited a reasonable antioxidant activity in the DPPH and phosphomolybdenum assays, with the ethanol extract recording the highest activity of 27.681 <i>μ</i>g/mL and 696.7452 <i>μ</i>g/g AAE, respectively. The content of brucine in the extracts was determined to be 0.0177–0.1259 × 10^–3% w/v, whereas the herbal products tested had a content of 0.8950−2.5013 × 10^–3% w/v. These levels were below the toxicity threshold of brucine. The developed method could be used for the routine quality control of <i>A. nobilis</i> extracts and formulations.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138534417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical Validation of Smartphone Spectroscopic Technic Used in an Educational Kinetic Study","authors":"S. Raissi, F. Fakhfakh","doi":"10.1155/2023/3729633","DOIUrl":"https://doi.org/10.1155/2023/3729633","url":null,"abstract":"Use of smartphone-based spectroscopy is showing a constant growth since last year. It presents the advantage of being widely available for everyone. The most important thing is that it is still a low-cost method adapted to the education context. However, as all analytical methods, it should be validated to ensure the reliability of its results. In this study, we present the steps of the validation process with its statistical tests applied to the dosage of di-iode. Shapiro–Wilk test revealed that our method has a random character. Homogeneity of variance analyses using the Cochran test confirmed the precision of the method. The Fisher test revealed the linearity of the model of correlation between I2 concentration and the response. The relation between response and concentration is A = 1000 C + 0.002. From the parameters of the linear regression of the model, we deduced the limits of quantification and XLq = 4·10−5 mol·L−1 and XLd = 1·10−5 mol·L−1. Thanks to tightness of the sample, the method of I2 dosage was successfully applied in iodine quantification to monitor acetone iodination during time in the context of kinetic studies with minimum system trouble. Being low cost, this method can facilitate access to physical methods in educational laboratories.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"1 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41553770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Carbon Nanofiber-Modified Concrete in Industrial Building Design.","authors":"Tianjie Liu","doi":"10.1155/2023/2587551","DOIUrl":"10.1155/2023/2587551","url":null,"abstract":"<p><p>In order to explore the influence law and action mechanism of carbon nanofibers on the basic mechanical properties of concrete, the author proposes the mechanical properties and microscopic mechanism of carbon nanofiber-modified concrete. Concrete was prepared with different dosages of carbon nanofibers, and the compressive strength, flexural strength, and splitting strength of carbon nanofiber-modified concrete were tested, and the modification mechanism was explored. Experimental results show that an appropriate amount of carbon nanofibers can improve the mechanical properties of concrete. When the dosage is 0.3%, the mechanical properties of carbon nanofiber-modified concrete are the best, and its compressive strength, flexural strength, and split tensile strength are increased by 9.2%, 13.2%, and 17.5%, respectively, compared with plain concrete. Carbon nanofibers can form a three-dimensional network structure inside the concrete, which can improve the microscopic morphology of the concrete, enhance the toughness and integrity of the concrete, fill the pore defects inside the concrete, refine the pore size distribution, and consume part of the fracture failure energy when the concrete is damaged.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"2587551"},"PeriodicalIF":1.8,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9891823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10652081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription Factor E2F1 Regulates the Expression of ADRB2.","authors":"Juan Du,&nbsp;Feifei Rui,&nbsp;Zhongfen Hao,&nbsp;Yun Hang,&nbsp;Jin Shu","doi":"10.1155/2023/8210685","DOIUrl":"https://doi.org/10.1155/2023/8210685","url":null,"abstract":"<p><p>Adrenergic beta-2-receptor (ADRB2) is highly expressed in various tissue cells, affecting the susceptibility, development, and drug efficacy of diseases such as bronchial asthma and malignant tumor. However, the transcriptional regulatory mechanism of the human ADRB2 gene remains unclear. This study aimed to clarify whether E2F transcription factor 1 (E2F1) was involved in the transcriptional regulation of the human ADRB2 gene. First, the 5' flanking region of the human ADRB2 gene was cloned, and its activity was detected using A549 and BEAS-2B cells. Second, it was found that the overexpression of E2F1 could increase promoter activity by a dual-luciferase reporter gene assay. In contrast, treatment of knockdown of E2F1 significantly resulted in a decrease in its promoter activity. Moreover, mutation of the binding site of E2F1 greatly reduced the potential of human ADRB2 promoter transcriptional activity to be regulated by E2F1 overexpression and knockdown. Additionally, by real-time quantitative PCR and Western blot analysis, we demonstrated that overexpression of E2F1 elevated the ADRB2 mRNA expression and protein levels while si-E2F1 reduced its expression. Finally, the consequence of the chromatin immunoprecipitation assay showed that E2F1 was able to bind to the promoter region of ADRB2 in vivo. These results confirmed that E2F1 upregulated the expression of the human ADRB2 gene.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"8210685"},"PeriodicalIF":1.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9398971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}