Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing Anthocleista nobilis

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-05-30 DOI:10.1155/2023/6957519

Rahinatu Ali Bawa, John Nii Addotey, Abena Amponsaa Brobbey, James Oppong-Kyekyeku, Christopher Kodjo Hlordzi

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引用次数: 0

Abstract

Anthocleista nobilis is a common constituent in numerous conventional medications in West Africa. The stem bark of A. nobilis is known to contain brucine and is used to treat intestinal parasites, stomachaches, gonorrhoea, wounds, etc. The extensive use has led to high market value and adulteration of their herbal products. In this work, the antioxidant properties of extracts of A. nobilis are verified, and a validated RP-HPLC method is used to estimate the brucine content of extracts and products containing A. nobilis. Stem bark extracts from ethyl acetate, 96% v/v ethanol, 70% v/v ethanol, and water of A. nobilis were investigated for phytochemical content using standard methods and their antioxidant activity using DPPH and phosphomolybdenum assays. An RP-HPLC method was developed and validated using brucine as a reference standard. The optimized conditions of the developed RP-HPLC method include a Supelcosil C18 column with dimensions 150 × 4.6 mm and particle size of 3 μm, a mobile phase comprising of MeOH : 0.1% v/v HCOOH (65:35 %v/v ) which was injected at a flow rate of 0.8 mL/min, and an injection volume of 50 μL. The wavelength of detection was 274 nm. The developed method was validated as per ICH guidelines. The common phytochemicals among the various extracts were tannins and alkaloids. All extracts exhibited a reasonable antioxidant activity in the DPPH and phosphomolybdenum assays, with the ethanol extract recording the highest activity of 27.681 μg/mL and 696.7452 μg/g AAE, respectively. The content of brucine in the extracts was determined to be 0.0177–0.1259 × 10^–3% w/v, whereas the herbal products tested had a content of 0.8950−2.5013 × 10^–3% w/v. These levels were below the toxicity threshold of brucine. The developed method could be used for the routine quality control of A. nobilis extracts and formulations.

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用生物测定法和反相高效液相色谱法分析花青菜提取物和草药制品

高贵Anthocleista nobilis是西非许多常规药物中的常见成分。众所周知，黄芪的茎皮含有马钱子碱，用于治疗肠道寄生虫、胃痛、淋病、伤口等。其广泛使用导致其草药产品具有很高的市场价值和掺假现象。本研究验证了苦参提取物的抗氧化性，并采用RP-HPLC法测定了苦参提取物及其制品的马钱子碱含量。以乙酸乙酯、96% v/v乙醇、70% v/v乙醇和水为萃取物，采用标准方法测定其植物化学成分含量，采用DPPH和磷钼法测定其抗氧化活性。以马钱子碱为标准品，建立了反相高效液相色谱法。建立的反相高效液相色谱法的优化条件为:粒径为3 μm、尺寸为150 × 4.6 mm的Supelcosil C18色谱柱，流动相为MeOH: 0.1% v/v HCOOH (65: 35% v/v)，进样流速为0.8 mL/min，进样量为50 μL。检测波长为274 nm。所开发的方法按照ICH指南进行了验证。各种提取物中常见的植物化学物质是单宁和生物碱。在DPPH和磷钼试验中，各提取物均表现出较好的抗氧化活性，其中乙醇提取物的抗氧化活性最高，分别为27.681 μg/mL和696.7452 μg/g AAE。提取液中马钱子碱的含量为0.0177 ~ 0.1259 × 10-3% w/v，药材中马钱子碱的含量为0.8950 ~ 2.5013 × 10-3% w/v。这些水平低于马钱子碱的毒性阈值。该方法可用于黄芪提取物及制剂的常规质量控制。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.