International Journal of Bacteriology最新文献_第4页

Study of the Antibacterial Efficacy of Bainiku-Ekisu against Pathogens. 研究 Bainiku-Ekisu 对病原体的抗菌功效。

International Journal of Bacteriology Pub Date : 2014-01-01 Epub Date: 2014-10-28 DOI: 10.1155/2014/460395

Deng-Jye Yang, Hsin-Yi Chen, Shih-Chuan Liu

{"title":"Study of the Antibacterial Efficacy of Bainiku-Ekisu against Pathogens.","authors":"Deng-Jye Yang, Hsin-Yi Chen, Shih-Chuan Liu","doi":"10.1155/2014/460395","DOIUrl":"10.1155/2014/460395","url":null,"abstract":"The research was undertaken to determine the bacteriostatic effects of the concentrate of Japanese apricot juice (bainiku-ekisu), which is a popular health food in Taiwan and Japan, on Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, and Escherichia coli ATCC 25922. The results show that E. faecalis, S. aureus, and E. coli could be killed or inhibited by bainiku-ekisu at concentrations between 1.0 and 10.0 mg/mL. The minimum inhibitory concentration (MIC) was 1 mg/mL for all strains, and the minimum bactericidal concentrations (MBCs) were 5, 2.5, and 2.5 mg/mL for E. faecalis, S. aureus, and E. coli, respectively. Using the growth rate to calculate the MICs and MBCs, the MICs were 1.55, 1.43, and 0.97 mg/mL, and the MBCs were 2.59, 2.63, and 2.25 mg/mL for E. faecalis, S. aureus, and E. coli, respectively. According to the D values, E. faecalis and S. aureus exhibited lower resistance than E. coli at lower bainiku-ekisu concentrations (1.0 and 2.5 mg/mL), and the resistance of these two pathogens was better than that of E. coli at higher bainiku-ekisu concentrations (5.0 and 10.0 mg/mL). The Z values of the E. faecalis, S. aureus, and E. coli strains were 3.47, 4.93, and 11.62 mg/mL, respectively. ","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82125371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Assessment of Viability of M. Tuberculosis after Exposure to CPC Using Different Methods. 使用不同方法评估接触 CPC 后结核杆菌的存活率。

International Journal of Bacteriology Pub Date : 2014-01-01 Epub Date: 2014-01-28 DOI: 10.1155/2014/564109

Gomathi Sekar, R Lakshmi, N Selvakumar

{"title":"The Assessment of Viability of M. Tuberculosis after Exposure to CPC Using Different Methods.","authors":"Gomathi Sekar, R Lakshmi, N Selvakumar","doi":"10.1155/2014/564109","DOIUrl":"10.1155/2014/564109","url":null,"abstract":"Settings. National Institute for Research in Tuberculosis, Chennai. Objective. To assess the proportion of metabolically active cells of Mycobacterium tuberculosis after exposed to CPC using FDA-EB vital staining and viable counts on LJ medium. Mycolic acid content in M. tuberculosis after exposure to CPC was estimated using HPLC. Methods. Clinical isolates of M. tuberculosis and standard reference strain M. tuberculosis H37Rv were used for FDA-EB, viable count, and HPLC. Results. FDA/EB consistently stained 70-90% of log phase cells as green and the remaining cells as red-orange. After CPC treatment, 65-70% of the cells stained red-orange. The viability counts were comparable to 0-day controls. Synthesis of mycolic acids in mycobacteria was reduced when exposed to CPC using HPLC due to the decreased metabolic activity of the organisms. Conclusion. The cells are metabolically inactive during storage with CPC but these cells grew well on LJ medium after removal of CPC. The viability of M. tuberculosis was maintained in CPC with minimal reduction. Mycolic acid content was reduced if the cells of M. tuberculosis were treated with CPC for 7 days. All the above findings provide yet another evidence for the damage of cell wall of M. tuberculosis. ","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75555402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Medium for Pharmaceutical Grade Arthrospira 药用级节螺旋体的新培养基

International Journal of Bacteriology Pub Date : 2013-12-28 DOI: 10.1155/2013/203432

A. Amara, A. Steinbüchel

{"title":"New Medium for Pharmaceutical Grade Arthrospira","authors":"A. Amara, A. Steinbüchel","doi":"10.1155/2013/203432","DOIUrl":"https://doi.org/10.1155/2013/203432","url":null,"abstract":"The aim of this study is to produce a pharmaceutical grade single cell product of Arthrospira from a mixed culture. We have designed a medium derived from a combination between George's and Zarrouk's media. Our new medium has the ability to inhibit different forms of cyanobacterium and microalgae except the Chlorella. The medium and the cultivation conditions have been investigated to map the points where only Arthrospira could survive. For that, a mixed culture of pure Chlorella and Arthrospira (~90 : 10) has been used to develop the best medium composition that can lead to the enrichment of the Arthrospira growth and the inhibition of the Chlorella growth. To enable better control and to study its growth, an 80 l photobioreactor has been used. We have used high saline (2xA-St) medium which has been followed by in fermentor reducing its concentration to 1.5x. The investigation proves that Chlorella has completely disappeared. A method and a new saline medium have been established using a photobioreactor for in fermentor production of single cell Arthrospira. Such method enables the production of pure pharmaceutical grade Arthrospira for medicinal and pharmaceutical applications or as a single cell protein.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76205946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Inflammatory Responses to Salmonella Infections Are Serotype-Specific 沙门氏菌感染的炎症反应是血清型特异性的

International Journal of Bacteriology Pub Date : 2013-09-16 DOI: 10.1155/2013/168179

Z. Ktsoyan, K. Ghazaryan, G. Manukyan, A. Martirosyan, A. Mnatsakanyan, K. Arakelova, Z. Gevorgyan, Anahit Sedrakyan, A. Asoyan, A. Boyajyan, R. Aminov

引用次数: 8

Physiological Properties and Salmonella Growth Inhibition of Probiotic Bacillus Strains Isolated from Environmental and Poultry Sources 环境源和家禽源分离的益生菌芽孢杆菌的生理特性及对沙门氏菌生长的抑制作用

International Journal of Bacteriology Pub Date : 2013-05-26 DOI: 10.1155/2013/958408

A. Menconi, M. Morgan, N. Pumford, B. Hargis, G. Tellez

{"title":"Physiological Properties and Salmonella Growth Inhibition of Probiotic Bacillus Strains Isolated from Environmental and Poultry Sources","authors":"A. Menconi, M. Morgan, N. Pumford, B. Hargis, G. Tellez","doi":"10.1155/2013/958408","DOIUrl":"https://doi.org/10.1155/2013/958408","url":null,"abstract":"The objective of the present study was to describe the physiological properties of seven potential probiotic strains of Bacillus spp. Isolates were characterized morphologically, biochemically, and by 16S rRNA sequence analyses for identification. Tolerance to acidic pH, high osmotic concentrations of NaCl, and bile salts were tested. Isolates were also evaluated for their ability to metabolize different carbohydrates sources. The antimicrobial sensitivity profiles were determined. Inhibition of gastrointestinal Salmonella colonization in an avian model was also evaluated. Five strains of Bacillus were tolerant to acidic conditions (pH 2.0) and all strains were tolerant to a high osmotic pressure (NaCl at 6.5%). Moreover, all strains were able to tolerate concentration of 0.037% bile salts after 24 h of incubation. Three strains were able to significantly reduce Salmonella Typhimurium levels in the crop and in the ceca of broiler-type chickens. Among the 12 antibiotics tested for antibiotic resistance, all strains were resistant to bacitracin and susceptible to gentamycin, neomycin, ormethoprim, triple sulfa, and spectinomycin. Bacterial spore formers have been shown to prevent gastrointestinal diseases in animals and humans. The results obtained in this study show important characteristics to be evaluated when selecting Bacillus spp. candidates to be used as probiotics.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76584880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Decreased C3 Activation by the devR Gene-Disrupted Mycobacterium tuberculosis Strain in Comparison to the Wild-Type Strain 与野生型结核分枝杆菌相比，devR基因破坏结核分枝杆菌菌株降低C3激活

International Journal of Bacteriology Pub Date : 2013-05-18 DOI: 10.1155/2013/512481

V. Narayan Rao, S. Manivannan, J. Tyagi, V. D. Ramanathan

{"title":"Decreased C3 Activation by the devR Gene-Disrupted Mycobacterium tuberculosis Strain in Comparison to the Wild-Type Strain","authors":"V. Narayan Rao, S. Manivannan, J. Tyagi, V. D. Ramanathan","doi":"10.1155/2013/512481","DOIUrl":"https://doi.org/10.1155/2013/512481","url":null,"abstract":"Activation of the complement component C3 is an important step in the complement cascade, contributing to inflammatory mechanisms. Considerable research on gene-disrupted mycobacterial strains using animal models of tuberculosis infection has reported the roles of some of the mycobacterial genes during tuberculosis infection. The aim of the present study was to assess the pattern of complement activation by the devR gene-disrupted Mycobacterium tuberculosis H37Rv strain and compare with that by its wild-type strain. In vitro complement activation at the level of C3 by the gene-disrupted strain, its complemented strain, and wild-type strain was performed using solid-phase ELISA. It was observed that the ability of devR gene-disrupted M. tuberculosis H37Rv to activate C3 was significantly reduced in comparison to its wild-type strain (P < 0.05). In addition, C3 activation by the complemented devR mutant strain was almost similar to that of the wild strain, which indicated that the reduced ability to activate C3 could potentially be due to the deletion of devR gene. These findings indicate that the gene devR probably aids in complement activation and contributes to the inflammatory processes during tuberculosis infection.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85055590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients 马来西亚患者临床标本中百日咳博德氏菌的培养和终点PCR检测

International Journal of Bacteriology Pub Date : 2013-05-07 DOI: 10.1155/2013/324136

Tan Ting, R. Hashim, N. Ahmad, Khairul Hafizi Abdullah

引用次数: 5

Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum 人支原体、解脲支原体和细小支原体的快速PCR检测

International Journal of Bacteriology Pub Date : 2013-03-11 DOI: 10.1155/2013/168742

S. Cunningham, J. Mandrekar, J. Rosenblatt, Robin Patel

{"title":"Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum","authors":"S. Cunningham, J. Mandrekar, J. Rosenblatt, Robin Patel","doi":"10.1155/2013/168742","DOIUrl":"https://doi.org/10.1155/2013/168742","url":null,"abstract":"Objective. We compared laboratory developed real-time PCR assays for detection of Mycoplasma hominis and for detection and differentiation of Ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for M. hominis and Ureaplasma culture. Methods. 283 genitourinary specimens received in the clinical bacteriology laboratory for M. hominis and Ureaplasma species culture were evaluated. Nucleic acids were extracted using the Total Nucleic Acid Kit on the MagNA Pure 2.0. 5 μL of the extracts were combined with 15 μL of each of the two master mixes. Assays were performed on the LightCycler 480 II system. Culture was performed using routine methods. Results. M. hominis PCR detected 38/42 M. hominis culture-positive specimens, as well as 2 that were culture negative (sensitivity, 90.5%; specificity, 99.2%). Ureaplasma PCR detected 139/144 Ureaplasma culture-positive specimens, as well as 9 that were culture negative (sensitivity, 96.5%; specificity, 93.6%). Of the specimens that tested positive for Ureaplasma species, U. urealyticum alone was detected in 33, U. parvum alone in 109, and both in 6. Conclusion. The described PCR assays are rapid alternatives to culture for detection of M. hominis and Ureaplasma species, and, unlike culture, the Ureaplasma assay easily distinguishes U. urealyticum from parvum.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88640839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Modulation of Bacterial Multidrug Resistance Efflux Pumps of the Major Facilitator Superfamily. 主要促进剂超家族细菌多药耐药外排泵的调控。

International Journal of Bacteriology Pub Date : 2013-01-01 DOI: 10.1155/2013/204141

Sanath Kumar, Mun Mun Mukherjee, Manuel F Varela

引用次数: 124

Survival and Growth of Vibrio cholerae, Escherichia coli, and Salmonella Spp. in Well Water Used for Drinking Purposes in Garoua (North Cameroon). 加鲁阿（喀麦隆北部）饮用水井水中霍乱弧菌、大肠杆菌和沙门氏菌的存活与生长。

International Journal of Bacteriology Pub Date : 2013-01-01 Epub Date: 2013-08-07 DOI: 10.1155/2013/127179

Moussa Djaouda, Bouba Gaké, Daniel Ebang Menye, Serge Hubert Zébazé Togouet, Moïse Nola, Thomas Njiné

{"title":"Survival and Growth of Vibrio cholerae, Escherichia coli, and Salmonella Spp. in Well Water Used for Drinking Purposes in Garoua (North Cameroon).","authors":"Moussa Djaouda, Bouba Gaké, Daniel Ebang Menye, Serge Hubert Zébazé Togouet, Moïse Nola, Thomas Njiné","doi":"10.1155/2013/127179","DOIUrl":"10.1155/2013/127179","url":null,"abstract":"The ability of strains of faecal bacteria (Vibrio cholerae, Escherichia coli ATCC 25922, and four strains of Salmonella isolated, resp., from well water, pig, poultry, and human urine in Garoua) to survive or grow in well water microcosms was compared. Water samples were obtained from two wells in Garoua (north Cameroun). Autoclaving at 121°C for 15 min and filtration through 0.2 µm filter were used to make microcosms. Microcosms were constituted of unfiltered-autoclaved, filtered-nonautoclaved, and filtered-autoclaved well waters. Bacterial strains were inoculated at initial cell concentration of 3 Log10CFU/mL. All strains were able to survive/grow in used microcosms, and a maximal concentration of 5.61 Log10CFU/mL was observed. Survival abilities were strain and microcosm dependent. The declines were more pronounced in filtered-nonautoclaved water than in the other microcosms. E. coli and Salmonella sp. (poultry strain) lowered to undetectable levels (<1 Log10CFU/mL) after two days of water storage. V. cholera decreased over time, but surviving cells persisted for longer period in filtered-nonautoclaved water from well W1 (1.91 Log10CFU/mL) and well W2 (2.09 Log10CFU/mL). Competition for nutrients and/or thermolabile antimicrobial substances synthesized by \"ultramicrocells\" or by the autochthonous bacteria retained by the filter might affect the bacterial survival. ","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82454859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0