International Journal of Bacteriology最新文献_第4页

Physiological Properties and Salmonella Growth Inhibition of Probiotic Bacillus Strains Isolated from Environmental and Poultry Sources 环境源和家禽源分离的益生菌芽孢杆菌的生理特性及对沙门氏菌生长的抑制作用

International Journal of Bacteriology Pub Date : 2013-05-26 DOI: 10.1155/2013/958408

A. Menconi, M. Morgan, N. Pumford, B. Hargis, G. Tellez

{"title":"Physiological Properties and Salmonella Growth Inhibition of Probiotic Bacillus Strains Isolated from Environmental and Poultry Sources","authors":"A. Menconi, M. Morgan, N. Pumford, B. Hargis, G. Tellez","doi":"10.1155/2013/958408","DOIUrl":"https://doi.org/10.1155/2013/958408","url":null,"abstract":"The objective of the present study was to describe the physiological properties of seven potential probiotic strains of Bacillus spp. Isolates were characterized morphologically, biochemically, and by 16S rRNA sequence analyses for identification. Tolerance to acidic pH, high osmotic concentrations of NaCl, and bile salts were tested. Isolates were also evaluated for their ability to metabolize different carbohydrates sources. The antimicrobial sensitivity profiles were determined. Inhibition of gastrointestinal Salmonella colonization in an avian model was also evaluated. Five strains of Bacillus were tolerant to acidic conditions (pH 2.0) and all strains were tolerant to a high osmotic pressure (NaCl at 6.5%). Moreover, all strains were able to tolerate concentration of 0.037% bile salts after 24 h of incubation. Three strains were able to significantly reduce Salmonella Typhimurium levels in the crop and in the ceca of broiler-type chickens. Among the 12 antibiotics tested for antibiotic resistance, all strains were resistant to bacitracin and susceptible to gentamycin, neomycin, ormethoprim, triple sulfa, and spectinomycin. Bacterial spore formers have been shown to prevent gastrointestinal diseases in animals and humans. The results obtained in this study show important characteristics to be evaluated when selecting Bacillus spp. candidates to be used as probiotics.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":"139 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76584880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Decreased C3 Activation by the devR Gene-Disrupted Mycobacterium tuberculosis Strain in Comparison to the Wild-Type Strain 与野生型结核分枝杆菌相比，devR基因破坏结核分枝杆菌菌株降低C3激活

International Journal of Bacteriology Pub Date : 2013-05-18 DOI: 10.1155/2013/512481

V. Narayan Rao, S. Manivannan, J. Tyagi, V. D. Ramanathan

{"title":"Decreased C3 Activation by the devR Gene-Disrupted Mycobacterium tuberculosis Strain in Comparison to the Wild-Type Strain","authors":"V. Narayan Rao, S. Manivannan, J. Tyagi, V. D. Ramanathan","doi":"10.1155/2013/512481","DOIUrl":"https://doi.org/10.1155/2013/512481","url":null,"abstract":"Activation of the complement component C3 is an important step in the complement cascade, contributing to inflammatory mechanisms. Considerable research on gene-disrupted mycobacterial strains using animal models of tuberculosis infection has reported the roles of some of the mycobacterial genes during tuberculosis infection. The aim of the present study was to assess the pattern of complement activation by the devR gene-disrupted Mycobacterium tuberculosis H37Rv strain and compare with that by its wild-type strain. In vitro complement activation at the level of C3 by the gene-disrupted strain, its complemented strain, and wild-type strain was performed using solid-phase ELISA. It was observed that the ability of devR gene-disrupted M. tuberculosis H37Rv to activate C3 was significantly reduced in comparison to its wild-type strain (P < 0.05). In addition, C3 activation by the complemented devR mutant strain was almost similar to that of the wild strain, which indicated that the reduced ability to activate C3 could potentially be due to the deletion of devR gene. These findings indicate that the gene devR probably aids in complement activation and contributes to the inflammatory processes during tuberculosis infection.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85055590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients 马来西亚患者临床标本中百日咳博德氏菌的培养和终点PCR检测

International Journal of Bacteriology Pub Date : 2013-05-07 DOI: 10.1155/2013/324136

Tan Ting, R. Hashim, N. Ahmad, Khairul Hafizi Abdullah

引用次数: 5

Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum 人支原体、解脲支原体和细小支原体的快速PCR检测

International Journal of Bacteriology Pub Date : 2013-03-11 DOI: 10.1155/2013/168742

S. Cunningham, J. Mandrekar, J. Rosenblatt, Robin Patel

{"title":"Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum","authors":"S. Cunningham, J. Mandrekar, J. Rosenblatt, Robin Patel","doi":"10.1155/2013/168742","DOIUrl":"https://doi.org/10.1155/2013/168742","url":null,"abstract":"Objective. We compared laboratory developed real-time PCR assays for detection of Mycoplasma hominis and for detection and differentiation of Ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for M. hominis and Ureaplasma culture. Methods. 283 genitourinary specimens received in the clinical bacteriology laboratory for M. hominis and Ureaplasma species culture were evaluated. Nucleic acids were extracted using the Total Nucleic Acid Kit on the MagNA Pure 2.0. 5 μL of the extracts were combined with 15 μL of each of the two master mixes. Assays were performed on the LightCycler 480 II system. Culture was performed using routine methods. Results. M. hominis PCR detected 38/42 M. hominis culture-positive specimens, as well as 2 that were culture negative (sensitivity, 90.5%; specificity, 99.2%). Ureaplasma PCR detected 139/144 Ureaplasma culture-positive specimens, as well as 9 that were culture negative (sensitivity, 96.5%; specificity, 93.6%). Of the specimens that tested positive for Ureaplasma species, U. urealyticum alone was detected in 33, U. parvum alone in 109, and both in 6. Conclusion. The described PCR assays are rapid alternatives to culture for detection of M. hominis and Ureaplasma species, and, unlike culture, the Ureaplasma assay easily distinguishes U. urealyticum from parvum.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88640839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Modulation of Bacterial Multidrug Resistance Efflux Pumps of the Major Facilitator Superfamily. 主要促进剂超家族细菌多药耐药外排泵的调控。

International Journal of Bacteriology Pub Date : 2013-01-01 DOI: 10.1155/2013/204141

Sanath Kumar, Mun Mun Mukherjee, Manuel F Varela

引用次数: 124