International Journal of Bacteriology最新文献_第3页

Antibacterial Attributes of Apigenin, Isolated from Portulaca oleracea L. 马齿苋中芹菜素的抗菌特性研究。

International Journal of Bacteriology Pub Date : 2014-05-13 DOI: 10.1155/2014/175851

H. B. Nayaka, R. Londonkar, M. K. Umesh, Asha Tukappa

{"title":"Antibacterial Attributes of Apigenin, Isolated from Portulaca oleracea L.","authors":"H. B. Nayaka, R. Londonkar, M. K. Umesh, Asha Tukappa","doi":"10.1155/2014/175851","DOIUrl":"https://doi.org/10.1155/2014/175851","url":null,"abstract":"The flavonoid apigenin was isolated from aerial part of P. oleracea L. The dried sample of plant was powdered and subjected to soxhlet extractor by adding 80 mL of ethanol : water (70 : 30). The extract was centrifuged at 11000 rpm for 30 min; supernatant was taken for further use. The fraction was concentrated and subjected to PTLC. The R f value of isolated apigenin was calculated (0.82). Purified material was also subjected to its IR spectra, LC-MS, NMR, and HPLC for structural elucidation. The apigenin so-obtained was subjected to antibacterial activity on five pathogenic bacterial strains like Pseudomonas aeruginosa, Salmonella typhimurium, Proteus mirabilis, Klebsiella pneumoniae and Enterobacter aerogenes; among all the bacterial strains, Salmonella typhimurium (17.36 ± 0.18) and Proteus mirabilis (19.12 ± 0.01) have shown maximum diameter of inhibition zone for flavonoid and remaining bacterial strains have shown moderate diameter of inhibition zone when compared with control values 14.56 ± 0.21 and 11.68 ± 0.13, respectively. The minimum inhibitory concentration (MIC) of the flavonoid isolated from P. oleracea L. was tested at the concentration ranging from undiluted sample to 10 mg per mL of concentration. The minimum inhibition concentration (MIC) for the flavonoid for all tested bacterial strains was found to be >4 mg per mL. Hence, the apigenin has antibacterial property and can be used to develop antibacterial drugs.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91323612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 90

Evaluation of the Pattern of EPIYA Motifs in the Helicobacter pylori cagA Gene of Patients with Gastritis and Gastric Adenocarcinoma from the Brazilian Amazon Region 巴西亚马逊地区胃炎和胃腺癌患者幽门螺杆菌cagA基因EPIYA基序模式的评价

International Journal of Bacteriology Pub Date : 2014-04-24 DOI: 10.1155/2014/418063

Adenielson Vilar e Silva, Mario Ribeiro da Silva Junior, Ruth Maria Dias Ferreira Vinagre, Kemper Nunes do Santos, Renata Aparecida Andrade da Costa, A. A. Fecury, J. Quaresma, L. Martins

{"title":"Evaluation of the Pattern of EPIYA Motifs in the Helicobacter pylori cagA Gene of Patients with Gastritis and Gastric Adenocarcinoma from the Brazilian Amazon Region","authors":"Adenielson Vilar e Silva, Mario Ribeiro da Silva Junior, Ruth Maria Dias Ferreira Vinagre, Kemper Nunes do Santos, Renata Aparecida Andrade da Costa, A. A. Fecury, J. Quaresma, L. Martins","doi":"10.1155/2014/418063","DOIUrl":"https://doi.org/10.1155/2014/418063","url":null,"abstract":"The Helicobacter pylori is associated with the development of different diseases. The clinical outcome of infection may be associated with the cagA bacterial genotype. The aim of this study was to determine the EPIYA patterns of strains isolated from patients with gastritis and gastric adenocarcinoma and correlate these patterns with the histopathological features. Gastric biopsy samples were selected from 384 patients infected with H. pylori, including 194 with chronic gastritis and 190 with gastric adenocarcinoma. The presence of the cagA gene and the EPIYA motif was determined by PCR. The cagA gene was more prevalent in patients with gastric cancer and was associated with a higher degree of inflammation, neutrophil activity, and development of intestinal metaplasia. The number of EPIYA-C repeats showed a significant association with an increased risk of gastric carcinoma (OR = 3.79, 95% CI = 1.92–7.46, and P = 0.002). A larger number of EPIYA-C motifs were also associated with intestinal metaplasia. In the present study, infection with H. pylori strains harboring more than one EPIYA-C motif in the cagA gene was associated with the development of intestinal metaplasia and gastric adenocarcinoma but not with neutrophil activity or degree of inflammation.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87038815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification 基于过氧化物酶信号扩增的重组抗原ELISA检测Q热特异性抗体

International Journal of Bacteriology Pub Date : 2014-03-12 DOI: 10.1155/2014/707463

Hua-Wei Chen, Zhiwen Zhang, E. Glennon, W. Ching

引用次数: 8

Changing Trends in Prevalence and Antibiotics Resistance of Uropathogens in Patients Attending the Gondar University Hospital, Northwest Ethiopia 埃塞俄比亚西北部贡达尔大学医院尿路病原菌患病率和抗生素耐药性的变化趋势

International Journal of Bacteriology Pub Date : 2014-03-10 DOI: 10.1155/2014/629424

M. Tiruneh, Sisay Yifru, Mucheye Gizachew, K. Molla, Yeshambel Belyhun, F. Moges, Mengistu Endris

{"title":"Changing Trends in Prevalence and Antibiotics Resistance of Uropathogens in Patients Attending the Gondar University Hospital, Northwest Ethiopia","authors":"M. Tiruneh, Sisay Yifru, Mucheye Gizachew, K. Molla, Yeshambel Belyhun, F. Moges, Mengistu Endris","doi":"10.1155/2014/629424","DOIUrl":"https://doi.org/10.1155/2014/629424","url":null,"abstract":"Background. In most hospitals of developing countries, urinary tract infections are treated empirically because of lack of culture facilities. This leads to emergence of multiresistant uropathogens. Culturing and drug susceptibility testing are essential to guide therapy. Objectives. To assess changing prevalence and resistance pattern of uropathogens to commonly used antibiotics in a two-year study period. Methods. Urine specimens were collected and cultured. Uropathogens were identified by standard methods and tested for antibiotics resistance. Data were analyzed using SPSS version 16 statistical sofware. P value < 0.05 was considered statistically significant. Results. The commonest isolates in both the previous and present studies were E. coli, Klebsiella, CoNS, S. aureus, Proteus, and Citrobacter species. Previous isolates of Enterobacteriaceae were 100% sensitive to ciprofloxacin, whereas present isolates developed 31% to 60% resistance to it. Previous isolates were less resistant to gentamycin than the present ones. Multiresistance isolates were predominant in present study than previous ones. Conclusion. E. coli was predominant in the two study periods. Present isolates were more resistant than previous ones. Some previous isolates were 100% sensitive to ciprofloxacin, whereas present isolates were increasingly resistant. Ciprofloxacin and gentamicin have been recommended for empiric treatment of urinary tract infections.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87921305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

In Vitro Screening for Abiotic Stress Tolerance in Potent Biocontrol and Plant Growth Promoting Strains of Pseudomonas and Bacillus spp. 假单胞菌和芽孢杆菌强效生防促株非生物抗性的体外筛选

International Journal of Bacteriology Pub Date : 2014-03-06 DOI: 10.1155/2014/195946

G. Praveen Kumar, S. K. Mir, Hassan Ahmed, S. Desai, E. Leo, D. Amalraj, Abdul Rasul

{"title":"In Vitro Screening for Abiotic Stress Tolerance in Potent Biocontrol and Plant Growth Promoting Strains of Pseudomonas and Bacillus spp.","authors":"G. Praveen Kumar, S. K. Mir, Hassan Ahmed, S. Desai, E. Leo, D. Amalraj, Abdul Rasul","doi":"10.1155/2014/195946","DOIUrl":"https://doi.org/10.1155/2014/195946","url":null,"abstract":"Plant growth promoting rhizobacteria (PGPR) has been identified as a group of microbes that are used for plant growth enhancement and biocontrol for management of plant diseases. The inconsistency in performance of these bacteria from laboratory to field conditions is compounded due to the prevailing abiotic stresses in the field. Therefore, selection of bacterial strains with tolerance to abiotic stresses would benefit the end-user by successful establishment of the strain for showing desired effects. In this study we attempted to isolate and identify strains of Bacillus and Pseudomonas spp. with stress tolerance and proven ability to inhibit the growth of potential phytopathogenic fungi. Screening of bacterial strains for high temperature (50°C), salinity (7% NaCl), and drought (−1.2 MPa) showed that stress tolerance was pronounced less in Pseudomonas isolates than in Bacillus strains. The reason behind this could be the formation of endospores by Bacillus isolates. Tolerance to drought was high in Pseudomonas strains than the other two stresses. Three strains, P8, P20 and P21 showed both salinity and temperature tolerance. P59 strain possessed promising antagonistic activity and drought tolerance. The magnitude of antagonism shown by Bacillus isolates was also higher when compared to Pseudomonas strains. To conclude, identification of microbial candidate strains with stress tolerance and other added characteristic features would help the end-user obtain the desired beneficial effects.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85086419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 73

Mixture of Sodium Hypochlorite and Hydrogen Peroxide on Adhered Aeromonas hydrophila to Solid Substrate in Water: Impact of Concentration and Assessment of the Synergistic Effect 次氯酸钠和双氧水混合对水中固体底物粘附的嗜水气单胞菌的影响:浓度的影响及协同效应的评价

International Journal of Bacteriology Pub Date : 2014-03-03 DOI: 10.1155/2014/121367

Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, O. V. Noah Ewoti, M. Nougang, Marlyse L. Moungang, G. Bricheux, M. Nola, T. Sime-Ngando

{"title":"Mixture of Sodium Hypochlorite and Hydrogen Peroxide on Adhered Aeromonas hydrophila to Solid Substrate in Water: Impact of Concentration and Assessment of the Synergistic Effect","authors":"Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, O. V. Noah Ewoti, M. Nougang, Marlyse L. Moungang, G. Bricheux, M. Nola, T. Sime-Ngando","doi":"10.1155/2014/121367","DOIUrl":"https://doi.org/10.1155/2014/121367","url":null,"abstract":"The synergistic effects of the combined treatments of NaOCl and H2O2 on the elimination of A. hydrophila adhered to polythene under static and dynamic conditions were evaluated. The concentrations 0.1, 0.2, and 0.3‰ NaOCl and 0.5, 1, and 1.5‰ H2O2 were used. The contact periods were 180, 360, 540, and 720 minutes. The abundance of cells adhered reached 2.47 and 2.27 units (log (CFU/cm²)), respectively, under static and dynamic conditions after action of the mixture of disinfectants, whereas it reached 2.41 and 3.39 units (log (CFU/cm²)) after action of NaOCl and H2O2 alone, respectively. Increase in the incubation period resulted in a significant decrease in the abundance of cells adhered when the mixture of 0.3‰ NaOCl and 1.5‰ H2O2 was used (P < 0.01). For each cell growth phase, there was a significant difference amongst the mean densities of cells adhered after action of the mixture of disinfectants (P < 0.05). Although the Freundlich isotherm parameters relatively varied from one experimental condition to another, the K f value registered in the exponential growth phase was relatively higher in static state than in dynamic regime; cells adhered under dynamic condition seem more sensitive to the synergistic action than those adhered under static condition.","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86413068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Antibiofilm Activity of Manuka Honey in Combination with Antibiotics 麦卢卡蜂蜜与抗生素联合使用的抗菌膜活性研究

International Journal of Bacteriology Pub Date : 2014-02-26 DOI: 10.1155/2014/795281

Michelle E. M. Campeau, Robin Patel

引用次数: 44

Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine Bacillus spp. of Eastern Coastal Plain of Tamil Nadu 泰米尔纳德邦东部沿海平原海洋芽孢杆菌的系统发育框架及生物表面活性剂基因表达分析

International Journal of Bacteriology Pub Date : 2014-02-12 DOI: 10.1155/2014/860491

Sreethar Swaathy, V. Kavitha, Arockiasamy Sahaya Pravin, G. Sekaran, A. Mandal, A. Gnanamani

引用次数: 17

Bacterial Biodegradation of Crude Oil Using Local Isolates 利用本地分离物对原油进行细菌生物降解

International Journal of Bacteriology Pub Date : 2014-01-20 DOI: 10.1155/2014/863272

Raed S. Al-Wasify, S. Hamed

引用次数: 85

Isolation and Antimicrobial Susceptibility Patterns of Campylobacter Species among Diarrheic Children at Jimma, Ethiopia. 埃塞俄比亚吉马市腹泻儿童弯曲杆菌的分离和抗菌药敏感性模式。

International Journal of Bacteriology Pub Date : 2014-01-01 Epub Date: 2014-01-11 DOI: 10.1155/2014/560617

Belay Tafa, Tsegaye Sewunet, Haimanot Tassew, Daniel Asrat

{"title":"Isolation and Antimicrobial Susceptibility Patterns of Campylobacter Species among Diarrheic Children at Jimma, Ethiopia.","authors":"Belay Tafa, Tsegaye Sewunet, Haimanot Tassew, Daniel Asrat","doi":"10.1155/2014/560617","DOIUrl":"10.1155/2014/560617","url":null,"abstract":"<p><p>Introduction. Campylobacter is one of the leading bacterial causes of food-borne disease. The prevalence of Campylobacter species resistant to antimicrobial agents is increasing. This study is intended to determine prevalence and antimicrobial susceptibility patterns of Campylobacter species among under-five children with diarrhea. Methodology. A cross-sectional study was conducted among 227 under-five children with diarrhea from July to October 2012 at Jimma town. Isolation and identification of Campylobacter species were performed using standard bacteriological techniques. Antimicrobial susceptibility test was performed following standard protocol. Chi-square and Fisher's exact tests were used for analysis. Results. From 227 under-five children, 16.7% were positive for Campylobacter spp.; isolates, C. jejuni, C. coli, and C. lari, accounted for 71.1%, 21.1%, and 7.9%, respectively. Higher rate of resistance was observed to ampicillin 76.3%, trimethoprim-sulfamethoxazole (68.4%), tetracycline (39.5%), chloramphenicol (31.6%), clindamycin (26.3%), and doxycycline (23.7%). Erythromycin, ciprofloxacin, gentamicin, norfloxacin, and nalidixic acid were effective for more than 80% of the isolates. Multiple drug resistance was observed among 78.9% of all the three spp. Conclusions. Isolation rate of Campylobacter spp. was high. C. lari was reported for the first time at this study area. Higher rate of resistance was observed to the commonly used drugs. </p>","PeriodicalId":13886,"journal":{"name":"International Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83522767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0