International Journal of Applied Research in Veterinary Medicine最新文献_第2页

Histomorphological and immunohistochemical studies of the ovary during dry and rainy seasons in Small East African goats (Capra hircus) 东非小山羊(Capra hircus)干雨季节卵巢的组织形态学和免疫组织化学研究

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-08-13 DOI: 10.31893/avr.2023013

J.A. Ngou, W. Kimaro, C. Luziga

{"title":"Histomorphological and immunohistochemical studies of the ovary during dry and rainy seasons in Small East African goats (Capra hircus)","authors":"J.A. Ngou, W. Kimaro, C. Luziga","doi":"10.31893/avr.2023013","DOIUrl":"https://doi.org/10.31893/avr.2023013","url":null,"abstract":"This study aimed to investigate the histomorphological and immunohistochemical changes in the ovaries of Small East African goats during the dry and rainy seasons. A total of 180 ovaries were subjected to analysis using hematoxylin and eosin staining and immunohistochemistry for desmin and smooth muscle actin as cytoskeletal components. The results demonstrated the presence of healthy and atretic primordial, primary, secondary, and antral follicles in the ovaries during the dry and rainy seasons. In the dry season, the proportions of healthy primordial, primary, secondary, and antral follicles were 236 (95%), 85 (97%), 25 (78%), and 6 (27%), respectively, whereas the atretic follicles accounted for 12 (5%), 3 (3%), 7 (22%), and 16 (73%). Conversely, during the rainy season, 157 (99%), 61 (98%), 48 (93%), and 31 (92%) were classified as healthy follicles, while 2 (1%), 1 (2%), 4 (7%), and 3 (8%) were categorized as atretic. There was a significant difference in healthy and atretic follicles between the dry and rainy seasons (P < 0.05). Immunohistochemical analysis revealed positive staining for both desmin and smooth muscle actin in the theca cells of secondary and antral follicles, cortical stroma, and tunica media of blood vessels in healthy and atretic follicles during dry and rainy seasons. However, staining was not observed in the granulosa cells and oocytes. Overall, this study indicates that most antral follicles undergo obliterated follicular atresia during the dry season.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"196 11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78344127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gender determination by PCR assay for the sex-determining region Y (SRY) gene amplification in Linnaeus’s two-toed sloth (Choloepus didactylus) 林奈二趾树懒性别决定区Y (SRY)基因扩增的PCR鉴定

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-06-15 DOI: 10.30782/jrvm.1283245

S. Ardıçlı, B. Bozkurt, Ezgi Vatansever

{"title":"Gender determination by PCR assay for the sex-determining region Y (SRY) gene amplification in Linnaeus’s two-toed sloth (Choloepus didactylus)","authors":"S. Ardıçlı, B. Bozkurt, Ezgi Vatansever","doi":"10.30782/jrvm.1283245","DOIUrl":"https://doi.org/10.30782/jrvm.1283245","url":null,"abstract":"In Linnaeus’s two-toed sloths (Choloepus didactylus), there is no distinct sexual dimorphism. It is an obstacle for gender determination from the external genitalia, especially in newborns or young sloths. Hence, easy, rapid, and reliable genetics-based methods for gender identification of the sloths are needed to continue captive breeding more successfully. In this study, a PCR-based technique that allows gender determination of two-toed sloths by using a sex-determining region Y (SRY) gene marker was described. The hair samples from young (suspect gender) and adult sloths (known gender) were used in genetic analysis. Initially, genomic DNA was isolated from hair root samples using Roche high pure PCR template preparation kit. The SRY primers were specifically designed based on the NCBI and Ensembl databases, and they were verified with the BLAST program concerning the two-toed sloth genome. PCR amplification with the SRY-specific primers was carried out by a programmable thermal cycler device using FastStart High Fidelity PCR System, Roche dNTPack. The samples were then electrophoresed on 2% agarose gels and were visualized by a gel documentation and analysis system. A specific band in the electrophoresis pattern is diagnostic for a male individual with a partial SRY region. Hence, the analysis demonstrated that the samples belonged to a male two-toed sloth. Two-toed sloth species are commonly preferred animals in zoos. Gender determination is inevitable for these animals in captivity to be raised successfully and healthily. Molecular genetic techniques allow high efficiency in taxonomic evaluations and gender identification in species that do not display sexual dimorphism. The PCR assay described here may be helpful for a rapid genetic analysis that can be widely used in gender determination for two-toed sloths.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76381594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Different Insemination Doses on Pregnancy Success in East Frisian Sheep 不同授精剂量对东弗里斯兰绵羊妊娠成功率的影响

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-06-12 DOI: 10.30782/jrvm.1250057

A. Aktar, Hasan Özkan, Kemal Bagci, Ümit Can Uzun, Mustafa Akkaşoğlu, Selvi Ali̇şan, H. Sagirkaya, S. Alçay

{"title":"The Effect of Different Insemination Doses on Pregnancy Success in East Frisian Sheep","authors":"A. Aktar, Hasan Özkan, Kemal Bagci, Ümit Can Uzun, Mustafa Akkaşoğlu, Selvi Ali̇şan, H. Sagirkaya, S. Alçay","doi":"10.30782/jrvm.1250057","DOIUrl":"https://doi.org/10.30782/jrvm.1250057","url":null,"abstract":"Saha şartlarında sperma almak ve tohumlama yapmak her zaman aynı koşullarda ve temiz şartlarda yapılamamaktadır. Bu çalışmada saha şartlarında üç farklı dozda intraservikal yolla suni tohumlama uygulaması sonucunda gebelik oranlarının araştırılması amaçlandı. Araştırmada Doğu Friz ırkı 60 adet koyun 3 gruba ayrıldı (n=20) ve 3 adet koç kullanıldı. Koyunlarda kızgınlıkların senkronize edilmesi amacıyla 20 mg flugeston asestat emdirilmiş süngerler 3 gruba da 12 gün süreyle yerleştirildi. Süngerlerin çıkarılacağı gün ovulasyonları senkronize etmek için tüm koyunlara 500 IU PMSG enjeksiyonu yapıldı ve 24 saat içerisinde tüm koyunların östrus gösterdiği tespit edildi. Aynı çiftlikteki aynı ırk 3 koçtan alınan spermalarla östrustaki koyunlar intraservikal yolla tohumlandı. Tohumlama dozları 1.grup için 100x106 spermatozoa/ml, 2.grup için 200x106 spermatozoa/ml, 3.grup için 400x106 spermatozoa/ml olarak belirlendi. Tohumlamaların ardından toplamda 28 adet (%47) koyunda fertilizasyon olmadığı ve tekrar kızgınlık gösterdiği, diğer 32 adet (%53) koyunun gebe kaldığı tespit edildi. Gerçekleştirilen istatistiksel analiz sonucunda 3.Grup (400x106) 1. Gruba (100x106) göre anlamlı olarak daha başarılı bulunmuştur (P","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83254109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eimeriosis Prevalence and Species Identifications in Lambs in Balıkesir City Center and Its Districts Balıkesir市中心及地区羔羊艾美拉虫病流行及种类鉴定

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-06-11 DOI: 10.30782/jrvm.1105726

Mehmet Özüiçli, A. I. Diker

{"title":"Eimeriosis Prevalence and Species Identifications in Lambs in Balıkesir City Center and Its Districts","authors":"Mehmet Özüiçli, A. I. Diker","doi":"10.30782/jrvm.1105726","DOIUrl":"https://doi.org/10.30782/jrvm.1105726","url":null,"abstract":"Bu çalışma Balıkesir ilinde kuzulardaki Eimeriosis türlerinin tayini ve yaygınlığının belirlenmesi amacıyla yapılmıştır. Balıkesir merkez, Edremit, Bigadiç ve Bandırma’da bulunan dört farklı çiftlikten, her birinden 150’şer adet olmak üzere toplamda 600 adet dışkı örneği toplanmıştır. Çalışma konusu olan kuzular 30-60, 60-90, 90-120 gün yaş aralığındadır. Her yaş aralığı için 25 erkek, 25 dişi olacak şekilde toplamda 50 kuzu seçilmiştir. Altı yüz hayvanın 438 adetinde Eimeriosis tespit edilmiştir. Balıkesir merkezde bulunan 150 hayvanın 110 tanesi, Edremit’te bulunan 150 hayvanın 108 tanesi, Bigadiç’te bulunan 150 hayvanın 96 tanesi, Bandırma’da bulunan 150 hayvanın 124 tanesi Eimeria pozitif bulunmuştur. Yedi farklı Eimeria türü dört çiftlikte de gözlemlenmiştir. Eimeria pozitif olan hayvanlarda en az 3, en fazla 6 Eimeria türü ile çoklu enfeksiyonlar saptanmıştır. Patojenik türler olan Eimeria ahsata, Eimeria ovinoidalis ve Eimeria bakuensis, Balıkesir bölgesinde tespit edilmiştir. Pozitif bulunan 438 hayvanın 367 tanesinde Eimeria ahsata (% 83,78), 331 tanesinde Eimeria parva (%75,57), 310 tanesinde Eimeria ovinoidalis (% 70,77), 306 tanesinde Eimeria bakuensis (% 69,86), 278 tanesinde Eimeria faueri (% 63,47), 260 tanesinde Eimeria intricata (% 59,36), 250 tanesinde Eimeria pallida (% 57,07) oranlarında tespit edilmiştir. Elde edilen sonuçlara göre E. ahsata (% 83,78) en fazla, E. pallida (% 57,07) en az gözlemlenen türler olmuştur. Bu çalışma ile Balıkesir bölgesinde kuzularda ilk kez Eimeria yaygınlık oranı belirlenmiş ve Eimeria tür tanımları yapılmıştır.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85749101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preventive effects of bovine gallstone on murine colon neoplasia: room for improvement? 牛胆石对小鼠结肠肿瘤的预防作用:有待改进?

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-05-04 DOI: 10.30782/jrvm.985209

I. Demirkan, Ece Kavas, M. Bozkurt, M. Korkmaz, İ. Kiliç, T. Kavas

{"title":"Preventive effects of bovine gallstone on murine colon neoplasia: room for improvement?","authors":"I. Demirkan, Ece Kavas, M. Bozkurt, M. Korkmaz, İ. Kiliç, T. Kavas","doi":"10.30782/jrvm.985209","DOIUrl":"https://doi.org/10.30782/jrvm.985209","url":null,"abstract":"Bovine gallstone (BGS) has been suggested to be used in the treatment of many diseases including neoplastic maladies in traditional medicine. Thus, this study aimed at evaluating the possible protective effects of BGS in mice colon tumor model. In the experiment, 8 week-old 30 male BALB/c mice weighing 25-30 g were randomly allocated into control group (n=6), tumor group ((n=6; tumor induced by 1,2- dimethylhydrazine? [DMH]), BGS>DMH group (n=6; BGS given prior to DMH challenge), BGS//DMH group (n=6; simultaneous administration of BGS and DMH) and DMH>BGS group (n=6; tumor induced by DMH then BGS given). Colonic tumor was induced by weekly subcutaneous injection of DMH at a 20 mg/kg/mouse dose for consequetive 11 weeks. BGS preparations were given to mice cyclically. Atypical cryptic foci formation in the colon was examined by histopathological staining. The lowest rate of colon tumor was noted in BGS>DMH group where BGS was given prior to tumor challenge. In addition simultaneous administration of DMH and BGS to mice inhibited tumor formation and development more significantly than those in other groups (except for BGS>DMH group). It was concluded that BGS significantly prevented colonic tumor formation if given to subjects before or during tumor challenge however, therapeutic capacity of BGS was not promising in this study.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85399021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Climate change, nutrigenomics, immune function and diseases and production in dairy cattle: a comprehensive review 气候变化、营养基因组学、奶牛免疫功能、疾病和生产:综合综述

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-04-15 DOI: 10.31893/avr.2023010

H. Musa, J. Mwacharo, Saber Y. Adam, Demin Cai, Abdelkareem A. Ahmed

引用次数: 1

Genetic polymorphism of the BMP15 gene in West African sheep (Fulani) breed in Sudan 苏丹西非富拉尼羊BMP15基因的遗传多态性

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-03-17 DOI: 10.31893/avr.2023009

Khalied H. Elshareef, H. Musa, Saber Y. Adam, Jaafar S. Fedailb, Demin Cai, Abdelkareem A. Ahmed

引用次数: 0

Effect of vitamin C, electrolyte, and jaggery on transportation stress in different seasons on biochemical parameters of goats 不同季节维生素C、电解质和jaggery对运输应激对山羊生化指标的影响

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-03-10 DOI: 10.31893/avr.2023008

D. Gupta, G. Kashyap, M. Ashutosh, A. Yadav

引用次数: 0

Characterization and computational investigation of polymorphisms in the TIR domain of the TLR4 protein in Turkish Holstein cattle. 土耳其荷斯坦牛TLR4蛋白TIR结构域多态性的表征和计算研究

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-03-01 DOI: 10.30782/jrvm.1197130

Sertaç Atalay

{"title":"Characterization and computational investigation of polymorphisms in the TIR domain of the TLR4 protein in Turkish Holstein cattle.","authors":"Sertaç Atalay","doi":"10.30782/jrvm.1197130","DOIUrl":"https://doi.org/10.30782/jrvm.1197130","url":null,"abstract":"Increasing environmental temperatures as a result of climate change adversely affect livestock welfare and health. Moreover, temperatures increase the distribution and survival of parasites and infectious agents. Livestock diseases that cause significant economic losses are a worldwide concern. Toll like receptor 4 (TLR4) is an ideal marker gene candidate, due to the key role it plays in initiating the immune response against pathogens. In this study, the toll-interleukin-1 receptor (TIR) domain polymorphisms were investigated in the Turkish Holstein cattle by DNA sequencing. The effects of polymorphisms on the protein structure and function were evaluated by computational tools (I-Mutant Suite, Project Hope and PyMOL). The rs8193069 C>T polymorphism was detected in the TIR domain. The SNP causes threonine to isoleucine substitution at position 674 of the TLR4 protein which is the second amino acid of the TIR domain. The I-Mutant Suite predicted that Thr674Ile substitution can result in decreased protein stability (DDG= -0.40 Kcal/mol). Project Hope results showed that mutant and wild-type amino acids have different properties and that this can disturb the TIR domain. The Thr674Ile polymorphism observed in the TIR domain of TLR4 protein in Turkish Holstein cattle might affect the function of the TLR4 by causing physicochemical changes.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85897694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Glucose Levels in Blood Samples Taken from Coccygeal, Jugular and Mammary Vein of Cows in Lactation 哺乳期奶牛尾骨、颈静脉和乳腺静脉血液中葡萄糖水平的比较

4区农林科学

International Journal of Applied Research in Veterinary Medicine Pub Date : 2023-01-23 DOI: 10.30782/jrvm.1195467

H. Ci̇han, S. Kasap, G. Akgül, Fatma Zehra Evci̇, S. Şentürk

引用次数: 0