Innate Immunity最新文献_第4页

Increased circulating monocyte MDSCs positively correlate with serum Interleukin-10 in metastatic melanoma patients. 在转移性黑色素瘤患者中，循环单核细胞MDSCs增加与血清白细胞介素-10呈正相关。

IF 3.2 4区医学

Innate Immunity Pub Date : 2023-04-01 DOI: 10.1177/17534259231172079

Katarina Mirjačić Martinović, Ana Vuletić, Nevena Tišma Miletić, Milica Nedeljković, Nada Babović, Suzana Matković, Vladimir Jurišić

{"title":"Increased circulating monocyte MDSCs positively correlate with serum Interleukin-10 in metastatic melanoma patients.","authors":"Katarina Mirjačić Martinović, Ana Vuletić, Nevena Tišma Miletić, Milica Nedeljković, Nada Babović, Suzana Matković, Vladimir Jurišić","doi":"10.1177/17534259231172079","DOIUrl":"https://doi.org/10.1177/17534259231172079","url":null,"abstract":"Numerous immunosuppressive cells such as myeloid-derived suppressor cells (MDSCs) and inhibitory cytokines identified in melanoma microenvironment have the important role in immune escape. Therefore, in this study we analyzed monocytic (m)MDSCs in peripheral blood of metastatic melanoma (MM) patients. In peripheral blood of 35 MM patients and 30 healthy controls we analyzed percentage of CD14 + HLA-DR- mMDSCs in monocyte gate and the mean fluorescence intensity of Foxp3 in CD25 + CD4 + regulatory T cells by Flow cytometry. Serum levels of transforming growth factor beta, interferon-gamma, interleukin (IL)-6, IL-8, IL-10 are measured by ELISA assays. In this study MM patients have significantly higher percentage of CD14 + HLA-DR- mMDSCs, as well as increased the baseline mMDSC/PBMC subset (NK, T, B cells, monocytes) ratio. Although there is no significant difference in the percentage of mMDSCs between groups of MM patients with different localization of distant metastasis, patients with elevated serum lactate dehydrogenase (LDH) have statistically significant higher percentage of these cells compared to LDH negative patients. Furthermore, in MM patients there is statistically significant positive correlation between values of IL-10 and the percentage of mMDSCs, only. Therefore, therapeutics that target circulating mMDSCs and IL-10 may have a big importance in the improvement of antitumor immunity in MM patients.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"29 3-4","pages":"37-44"},"PeriodicalIF":3.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c8/17/10.1177_17534259231172079.PMC10331212.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9825492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and validation of click-modified NOD1/2 agonists 点击修饰NOD1/2激动剂的合成与验证

IF 3.2 4区医学

Innate Immunity Pub Date : 2023-03-28 DOI: 10.1101/2023.03.28.534546

R. Bharadwaj, Madison V. Anonick, S. Mashayekh, Ashley R. Brown, Kimberly A. Wodzanowski, K. Okuda, N. Silverman, C. Grimes

引用次数: 1

C/EBPβ deficiency enhances the keratinocyte innate immune response to direct activators of cytosolic pattern recognition receptors. C/EBPβ 缺乏会增强角质形成细胞对细胞膜模式识别受体直接激活剂的先天性免疫反应。

IF 3.2 4区医学

Innate Immunity Pub Date : 2023-01-01 Epub Date: 2023-04-24 DOI: 10.1177/17534259231162192

John S House, Sophia Gray, Jennifer R Owen, Dereje D Jima, Robert C Smart, Jonathan R Hall

{"title":"C/EBPβ deficiency enhances the keratinocyte innate immune response to direct activators of cytosolic pattern recognition receptors.","authors":"John S House, Sophia Gray, Jennifer R Owen, Dereje D Jima, Robert C Smart, Jonathan R Hall","doi":"10.1177/17534259231162192","DOIUrl":"10.1177/17534259231162192","url":null,"abstract":"The skin is the first line of defense to cutaneous microbes and viruses, and epidermal keratinocytes play a critical role in preventing infection by viruses and pathogens through activation of the type I interferon (IFN) response. Using RNAseq analysis, here we report that the conditional deletion of C/EBPβ transcription factor in mouse epidermis (CKOβ mice) resulted in the upregulation of IFNβ and numerous keratinocyte interferon-stimulated genes (ISGs). The expression of cytosolic pattern recognition receptors (cPRRs), that recognize viral RNA and DNA, were significantly increased, and enriched in the RNAseq data set. cPRRs stimulate a type I IFN response that can trigger cell death to eliminate infected cells. To determine if the observed increases in cPRRs had functional consequences, we transfected CKOβ primary keratinocytes with the pathogen and viral mimics poly(I:C) (dsRNA) or poly(dA:dT) (synthetic B-DNA) that directly activate PRRs. Transfected CKOβ primary keratinocytes displayed an amplified type I IFN response which was accompanied by increased activation of IRF3, enhanced ISG expression, enhanced activation of caspase-8, caspase-3 and increased apoptosis. Our results identify C/EBPβ as a critical repressor of the keratinocyte type I IFN response, and demonstrates that the loss of C/EBPβ primes keratinocytes to the activation of cytosolic PRRs by pathogen RNA and DNA to induce cell death mediated by caspase-8 and caspase-3.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"29 1-2","pages":"14-24"},"PeriodicalIF":3.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/75/28/10.1177_17534259231162192.PMC10164275.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9438653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyroptosis, inflammasome, and gasdermins in tumor immunity. 肿瘤免疫中的焦亡、炎性体和气真皮。

IF 3.2 4区医学

Innate Immunity Pub Date : 2023-01-01 DOI: 10.1177/17534259221143216

Xiaohu Ouyang, Junfen Zhou, Lan Lin, Zili Zhang, Shanshan Luo, Desheng Hu

引用次数: 6

SENP3 facilitates M1 macrophage polarization via the HIF-1α/PKM2 axis in lipopolysaccharide-induced acute lung injury. 在脂多糖诱导的急性肺损伤中，SENP3通过HIF-1α/PKM2轴促进M1巨噬细胞极化。

IF 3.2 4区医学

Innate Immunity Pub Date : 2023-01-01 DOI: 10.1177/17534259231166212

Shuangjun He, Chenyu Fan, Yiming Ji, Qian Su, Feng Zhao, Cuiying Xie, Xuelian Chen, Yang Zhang, Yi Chen

{"title":"SENP3 facilitates M1 macrophage polarization via the HIF-1α/PKM2 axis in lipopolysaccharide-induced acute lung injury.","authors":"Shuangjun He, Chenyu Fan, Yiming Ji, Qian Su, Feng Zhao, Cuiying Xie, Xuelian Chen, Yang Zhang, Yi Chen","doi":"10.1177/17534259231166212","DOIUrl":"https://doi.org/10.1177/17534259231166212","url":null,"abstract":"M1/M2 macrophage polarization plays a pivotal role in the development of acute lung injury (ALI). The hypoxia-inducible factor-1α/pyruvate kinase M2 (HIF-1α/PKM2) axis, which functions upstream of macrophage polarization, has been implicated in this process. The function of HIF-1α is known to be tightly regulated by SUMOylation. Upregulation of SUMO-specific peptidase 3 (SENP3), a deSUMOylation enzyme, is induced by reactive oxygen species (ROS), which are abundantly produced during ALI. To explore the links between SENP3, macrophage polarization, and lung injury, we used mice with Senp3 conditional knockout in myeloid cells. In the lipopolysaccharide (LPS)-induced ALI model, we found that in vitro and in vivo SENP3 deficiency markedly inhibited M1 polarization and production of pro-inflammatory cytokines and alleviated lung injury. Further, we demonstrated that SENP3 deficiency suppressed the LPS-induced inflammatory response through PKM2 in a HIF-1α-dependent manner. Moreover, mice injected with LPS after PKM2 inhibitor (shikonin) treatment displayed inhibition of M1 macrophage polarization and reduced lung injury. In summary, this work revealed that SENP3 promotes M1 macrophage polarization and production of proinflammatory cytokines via the HIF-1α/PKM2 axis, contributing to lung injury; thus, SENP3 may represent a potential therapeutic target for ALI treatment.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"29 1-2","pages":"25-34"},"PeriodicalIF":3.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/fd/10.1177_17534259231166212.PMC10164277.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9429638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

1103 Lupus clinical flares in patients with gut pathobiont blooms share a novel peripheral blood transcriptomic immune activation profile 1103 肠道病理性水华患者的狼疮临床发作具有一个新的外周血转录组免疫激活谱

IF 3.2 4区医学

Innate Immunity Pub Date : 2022-12-01 DOI: 10.1136/lupus-2022-lupus21century.68

G. Silverman, MacIntosh G. Cornwell, P. Izmirly, J. Buyon, Doua F. Azzouz, Kelly V. Ruggles

引用次数: 1

1005 Characterization of regulatory receptors on plasmacytoid dendritic cells in lupus 1005狼疮患者浆细胞样树突状细胞调节受体的研究

IF 3.2 4区医学

Innate Immunity Pub Date : 2022-12-01 DOI: 10.1136/lupus-2022-lupus21century.65

M. Jensen, Ilona Nln, T. Iwamoto, Jessica M. Dorschner, D. Vsetecka, Gabrielle A McCoy, J. L. Paredes, T. Niewold

引用次数: 0

1101 Urine complement activation products in lupus nephritis 尿补体激活产品在狼疮肾炎中的应用

IF 3.2 4区医学

Innate Immunity Pub Date : 2022-12-01 DOI: 10.1136/lupus-2022-lupus21century.66

Nicholas L. Li, D. Birmingham, L. Biederman, T. Nadasdy, B. Rovin

引用次数: 0

1102 The trajectory of glomerular and tubulointerstitial lesions after treatment of lupus nephritis 狼疮性肾炎治疗后肾小球和小管间质病变的发展轨迹

IF 3.2 4区医学

Innate Immunity Pub Date : 2022-12-01 DOI: 10.1136/lupus-2022-lupus21century.67

A. Malvar, V. Alberton, Bruno J Lococo, M. C. Lourenço, Joaquín Martínez, Mauro Elencwajg, H. Nagaraja, B. Rovin

引用次数: 0

1004 Altered ERα localization differentially modulates immune cell subsets 1004改变的ERα定位差异调节免疫细胞亚群

IF 3.2 4区医学

Innate Immunity Pub Date : 2022-12-01 DOI: 10.1136/lupus-2022-lupus21century.64

Cameron Leyers, Jena R. Wirth, M. Cunningham

引用次数: 0