Killer cell immunoglobulin-like receptor (KIR) genes and their HLA ligands in a Brazilian population.

IF 2.8 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Innate Immunity Pub Date : 2023-07-01 DOI:10.1177/17534259231178592

Daniela Maira Cardozo, Amanda Vansan Marangon, Fernando Guimarães, Silvia Marques, Sofia Lieber, Márcia Delamain, Francisco Aranha, Jeane Eliete Laguila Visentainer, Cármino Antonio de Souza

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Abstract

Killer cell immunoglobulin-like receptors (KIR) exhibit extensive diversity, giving rise to different KIR profiles in populations worldwide. This study aimed to investigate the distribution of KIR genes and HLA ligands in a population from Campinas, southeastern Brazil (n = 292), and to compare their results with other populations. A comprehensive analysis of population-specific genes, genotype, and haplotype frequencies of KIR may facilitate a better understanding of their evolution and role in immunity. The genotyping of 16 KIR genes and HLA class I alleles was performed by the reverse sequence-specific oligonucleotide methodology using the Luminex platform (One Lambda, Inc., Canoga Park, CA). The framework genes were present in all individuals, with the most common non-framework KIR genes detected being KIR2DP1(96.6%), KIR2DL1(95.5%), KIR3DL1(94.5%), KIR2DS4(93.8%) and KIR2DL3(87.3%). KIR2DS1, KIR2DS3, KIR2DS5, and KIR3DS1 presented frequencies below 40%. KIR2DL2, KIR2DL5, and KIR2DS2 showed intermediate frequencies (between53% and 58%). The activating gene KIR2DS5 was the least common in this population (30.8%). Forty-five KIR profiles were found with the commonest being the homozygous A haplotype (27.4%). The distribution of KIR genes in the Brazilian population is similar to Caucasian European and Euro-descendant populations.

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巴西人群中的杀伤细胞免疫球蛋白样受体(KIR)基因及其HLA配体

杀伤细胞免疫球蛋白样受体(KIR)表现出广泛的多样性，在世界范围内的人群中产生了不同的KIR谱。本研究旨在调查巴西东南部坎皮纳斯人群(n = 292)中KIR基因和HLA配体的分布，并将其结果与其他人群进行比较。对人群特异性基因、基因型和KIR单倍型频率的全面分析可能有助于更好地理解它们的进化和在免疫中的作用。使用Luminex平台(One Lambda, Inc.， Canoga Park, CA)，通过反序列特异性寡核苷酸方法对16个KIR基因和HLA I类等位基因进行基因分型。框架基因存在于所有个体中，最常见的非框架KIR基因为KIR2DP1(96.6%)、KIR2DL1(95.5%)、KIR3DL1(94.5%)、KIR2DS4(93.8%)和KIR2DL3(87.3%)。KIR2DS1、KIR2DS3、KIR2DS5和KIR3DS1的频率均低于40%。KIR2DL2、KIR2DL5和KIR2DS2表现为中频(53% ~ 58%)。激活基因KIR2DS5在该人群中最不常见(30.8%)。共发现45个KIR谱，其中最常见的是纯合子A单倍型(27.4%)。巴西人群的KIR基因分布与高加索欧洲人和欧洲后裔人群相似。

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来源期刊

Innate Immunity 生物-免疫学

CiteScore

7.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Innate Immunity is a highly ranked, peer-reviewed scholarly journal and is the official journal of the International Endotoxin & Innate Immunity Society (IEIIS). The journal welcomes manuscripts from researchers actively working on all aspects of innate immunity including biologically active bacterial, viral, fungal, parasitic, and plant components, as well as relevant cells, their receptors, signaling pathways, and induced mediators. The aim of the Journal is to provide a single, interdisciplinary forum for the dissemination of new information on innate immunity in humans, animals, and plants to researchers. The Journal creates a vehicle for the publication of articles encompassing all areas of research, basic, applied, and clinical. The subject areas of interest include, but are not limited to, research in biochemistry, biophysics, cell biology, chemistry, clinical medicine, immunology, infectious disease, microbiology, molecular biology, and pharmacology.