Biotechnology Journal最新文献_第9页

Covalent Immobilization of β-Glucosidase on Magnetic Spent Coffee Grounds Modified With PAMAM Dendrimers for Enhanced Glucose Tolerance PAMAM树状大分子修饰的磁咖啡渣共价固定β-葡萄糖苷酶增强葡萄糖耐量

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-16 DOI: 10.1002/biot.70025

Shaifali Bhardwaj, Ekta Naik, Akansha Tripathi, Debashish Ghosh, Anil Kumar Sinha

{"title":"Covalent Immobilization of β-Glucosidase on Magnetic Spent Coffee Grounds Modified With PAMAM Dendrimers for Enhanced Glucose Tolerance","authors":"Shaifali Bhardwaj, Ekta Naik, Akansha Tripathi, Debashish Ghosh, Anil Kumar Sinha","doi":"10.1002/biot.70025","DOIUrl":"10.1002/biot.70025","url":null,"abstract":"The successful application of enzymes in industries encounters challenges related to high costs, stability, and reuse. In this study, β-glucosidase (BGL) was immobilized via a covalent method after the synthesis of different generations (G0-2) of polyamidoamine (PAMAM) dendrimers modified magnetic spent coffee grounds (SCGs). With the increase in PAMAM generation, BGL immobilization (163.123 and 218.99 mg protein/g support in G1 and G2, respectively) on the support increased. Exceptional stability (> 80% residual activity) was observed for immobilized BGL at varying pH (3 to 6) and temperature (30°C to 80°C) after a 2 h incubation period. The higher generations of PAMAM dendrimers were more tolerant to glucose inhibition than the free enzyme. PAMAM G1 and G2 generations showed > 75% residual activity at 200 mM glucose concentration compared to free enzyme, with only 50% residual activity at 100 mM glucose concentration. After employing BGL@MSCG3@G0-2 for cellobiose hydrolysis, > 69% glucose yield for 10 cycles was maintained.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation and Biological Characterization of an Anti-IL-6Rα Biosimilar Candidate Antibody 抗il - 6r α生物类似物候选抗体的制备及生物学特性研究

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-16 DOI: 10.1002/biot.70017

Gisela García-Pérez, Gabriela García-Entenza, Arlem García Delgado, Arnelys Durán-Hernández, Tania Gómez Peña, Roberto Daniel Machado-Santisteban, Gertrudis Rojas, Tania Carmenate

{"title":"Generation and Biological Characterization of an Anti-IL-6Rα Biosimilar Candidate Antibody","authors":"Gisela García-Pérez, Gabriela García-Entenza, Arlem García Delgado, Arnelys Durán-Hernández, Tania Gómez Peña, Roberto Daniel Machado-Santisteban, Gertrudis Rojas, Tania Carmenate","doi":"10.1002/biot.70017","DOIUrl":"10.1002/biot.70017","url":null,"abstract":"<div>\u0000 \u0000 Tocilizumab is a monoclonal antibody (mAb) that recognizes human Interleukin 6 receptor alpha (IL-6Rα) and antagonizes IL-6 signaling. It is therefore used in the treatment of moderate to severe rheumatoid arthritis in patients with inadequate response to antirheumatic drugs, and in the treatment of systemic juvenile idiopathic arthritis. During the course of the Coronavirus Disease 2019 (COVID-19) pandemic, the potential application of Tocilizumab in severe inflammatory diseases was demonstrated. In addition, the drug is attracting increasing attention for its potential application in other pathologies, particularly cancers where the central role of Interleukin 6 (IL-6) has been described, such as pancreatic ductal adenocarcinoma. The necessity for further research in this area is far too great, which is why the development of additional biosimilars is both necessary and welcome. In this work, we describe the generation and biological characterization of a biosimilar candidate of Tocilizumab. A stable producing CHO-K1 cell line was obtained by lentiviral transduction. We demonstrated that the antibody generated, named 41TB, has similar size heterogeneity and electrophoretic profile as Tocilizumab, and high levels of purity. Moreover, 41TB exhibits comparable binding capacity to IL-6Rα, as well as the ability to interrupt IL-6/IL-6Rα binding in ELISA. Additionally, both antibodies were likewise able to inhibit DS-1 cell proliferation, IL-6 induced STAT3 phosphorylation, and C-Reactive Protein production in vitro. Galactose supplementation of the culture medium appears to be necessary to generate a glycosylation profile similar to the original antibody.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Research on the Treatment of Saline-Alkali Diseases of Murals in Arzhai Grottoes With Salt-Tolerant Mineralizing Bacteria 耐盐矿化菌治疗阿寨石窟壁画盐碱病的实验研究

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-16 DOI: 10.1002/biot.70044

Chi Li, Panshi Wang, Xiaorong Wang, Yu Gao, Wu Zhang

{"title":"Experimental Research on the Treatment of Saline-Alkali Diseases of Murals in Arzhai Grottoes With Salt-Tolerant Mineralizing Bacteria","authors":"Chi Li, Panshi Wang, Xiaorong Wang, Yu Gao, Wu Zhang","doi":"10.1002/biot.70044","DOIUrl":"10.1002/biot.70044","url":null,"abstract":"<div>\u0000 \u0000 The degradation of cave murals due to saline-alkali conditions still poses a formidable challenge in the realm of cultural heritage conservation. This research proposes a novel methodology grounded in microbial mineralization technology. By leveraging Bacillus oceanicus, a newly isolated strain, calcium carbonate precipitation is induced under saline-alkali conditions, forming a protective layer. Experimental outcomes demonstrate that, in contrast to the conventional strain Bacillus pasteurii, B. oceanicus showcases exceptional salt tolerance. Its salt tolerance level in an identical saline environment is twice as high as that of B. pasteurii. Additionally, B. oceanicus can significantly enhance the mechanical properties of specimens. When compared with B. pasteurii, the unconfined compressive strength experiences a 47.9% increment, the cohesion increases by 83.18%, and the internal friction angle rises by 29.06%. In the simulation experiments addressing the saline-alkali-related pathologies of murals, compared to the untreated samples, the efflorescence height, efflorescence powder volume, and salt crust thickness of the samples treated by MICP (microbially induced calcite precipitation) decrease by 29.1%, 45.0%, and 36.4%, respectively. These findings accentuate the potential of B. oceanicus as an efficient and environmentally-friendly solution for the preservation of murals in saline-alkali settings, offering a fresh research perspective for cultural heritage conservation.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chaperone-Tag System for Activity Improvement of RNA Polymerase in Thermophiles 提高嗜热菌RNA聚合酶活性的伴侣-标签系统

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-16 DOI: 10.1002/biot.70060

Chunxiao Wang, Chuan Li, Chengyu Zhang, Zhiheng Yang, Chengwei Liu, Weishan Wang

{"title":"Chaperone-Tag System for Activity Improvement of RNA Polymerase in Thermophiles","authors":"Chunxiao Wang, Chuan Li, Chengyu Zhang, Zhiheng Yang, Chengwei Liu, Weishan Wang","doi":"10.1002/biot.70060","DOIUrl":"10.1002/biot.70060","url":null,"abstract":"<div>\u0000 \u0000 Thermophilic microorganisms hold advantages including resistance to contamination, reduced cooling costs, and enhanced enzyme activity, positioning them as promising candidates for next-generation industrial biotechnology. However, the inactivity of tools developed for mesophiles hampered the development of thermophiles. By investigating the expression of the T7 RNA polymerase (T7RNAP) in Parageobacillus thermoglucosidasius, we found that the low expression levels of heat-shock proteins in thermophiles contribute to the inactivity. Specifically, we identified HSP33, DnaK/J, and GroS/L as key chaperones that synergistically enhance the folding of T7RNAP. Through understanding the potential recruitment effect of HSP33 on DnaK/J, we de novo designed an HSP33-based tag to improve the activity of T7RNAP to a greater extent. To further enhance this recruitment effect, we conducted a systematic collection of the core element HSP33 through evolutionary analysis across various thermophilic microorganisms, and screened a superior tag that significantly boosted the activity of T7RNAP. Ultimately, we demonstrated that the developed Chaperone-Tag system also improved the activity of T3 RNA polymerase in this strain, highlighting the broad applicability of our strategy.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design of an Epoxide Hydrolase From Spatholobus Suberectus: Enhancing Catalytic Activity and Thermostability for Efficient (R)-Styrene Oxide Production 鸡血藤环氧化物水解酶的合理设计：提高催化活性和热稳定性，高效生产(R)-苯乙烯氧化物

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-16 DOI: 10.1002/biot.70061

Xiafeng Lu, Yan Wu, Ning Li, Wen Zheng, Kai Liu, Feng Du, Zhibo Luo

{"title":"Rational Design of an Epoxide Hydrolase From Spatholobus Suberectus: Enhancing Catalytic Activity and Thermostability for Efficient (R)-Styrene Oxide Production","authors":"Xiafeng Lu, Yan Wu, Ning Li, Wen Zheng, Kai Liu, Feng Du, Zhibo Luo","doi":"10.1002/biot.70061","DOIUrl":"10.1002/biot.70061","url":null,"abstract":"<div>\u0000 \u0000 (R)-Styrene oxide is a high-value chiral intermediate in pharmaceutical and chemical industries, yet its enantioselective synthesis remains challenging. Here, we engineered an epoxide hydrolase from Spatholobus suberectus (SsEH) to address its limitations in catalytic activity and thermostability. Through a computational strategy integrating homology modeling, molecular dynamics (MD) simulations, and machine learning, we rationally designed a mutagenesis library and identified the quintuple variant SsEH-His41Arg-Thr71Val-Lys117Leu-Leu187Phe-Ser244Ala (SsEH-M5). This variant exhibited a 17.0-fold increase in catalytic activity and a 2.1-fold improvement in thermostability (half-life at 35°C) compared to wild-type SsEH. Structural analysis revealed that enhanced activity stemmed from optimized substrate binding and nucleophilic attack efficiency, while additional hydrogen bonds (Arg41-Tyr216-Asp212-His38) stabilized the enzyme's architecture. In a 3500 L bioreactor, SsEH-M5 catalyzed the enantioconvergent hydrolysis of 60 g/L racemic styrene oxide using 2.5 g/L (DCW, dry cell weight) whole-cell biocatalyst, yielding (R)-styrene oxide with >99.5% enantiomeric excess (ee) and (R)-1-phenyl-1,2-ethanediol (>96.0% ee). This work highlights the synergy of computational design and experimental validation in developing robust biocatalysts for industrial-scale chiral synthesis.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Growth of Bone Marrow-Derived Mesenchymal Stem Cells on the Microcarriers Tethered With Engineered Basic Fibroblast Growth Factor 骨髓间充质干细胞在工程碱性成纤维细胞生长因子微载体上的增强生长

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-11 DOI: 10.1002/biot.70057

Shiina Pasanen, Archana Mootha, Isao Hirata, Kotaro Tanimoto, Koichi Kato

{"title":"Enhanced Growth of Bone Marrow-Derived Mesenchymal Stem Cells on the Microcarriers Tethered With Engineered Basic Fibroblast Growth Factor","authors":"Shiina Pasanen, Archana Mootha, Isao Hirata, Kotaro Tanimoto, Koichi Kato","doi":"10.1002/biot.70057","DOIUrl":"10.1002/biot.70057","url":null,"abstract":"Microcarrier-based bioreactors have been studied extensively for the expansion of human mesenchymal stem cells (hMSCs) to be used in regenerative therapy. To maximize the expansion efficiency, we developed microcarriers that tethered basic fibroblast growth factor (bFGF) on their surfaces. Using recombinant DNA technologies, bFGF was fused with a collagen-binding peptide (CBP) and bound to microcarriers that had been coated with type I collagen. In this study, we examined two types of CBP: a 28-mer peptide derived from human osteopontin (CBP1) and an 11-mer peptide derived from human decorin (CBP2). The chimeric proteins, CBP1-bFGF and CBP2-bFGF, were prepared using a bacterial expression system. Their structures were analyzed by circular dichroism spectroscopy and computer-based structure prediction, and their biological functions were assessed in hMSC cultures. Based on the results of these experiments, refolding conditions were optimized to increase the yields of soluble and bioactive CBP1-bFGF and CBP2-bFGF. Microcarriers that were tethered with CBP1-bFGF and CBP2-bFGF promoted hMSC growth without considerably altering their surface marker expression and osteogenic differentiation ability. These findings highlighted the potential of bFGF-tethered microcarriers as essential bioreactor components for hMSC expansion.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tensile Forces and Nanofiber Alignment Influence Both Innervated and Non-Innervated Skeletal Myofiber Formation in Custom Mechanobioreactors 拉力和纳米纤维排列影响定制机械生物反应器中神经支配和非神经支配骨骼肌纤维的形成

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-09 DOI: 10.1002/biot.70047

Melanie C. Hilman, Foteini Mourkioti, Suradip Das, D. Kacy Cullen

{"title":"Tensile Forces and Nanofiber Alignment Influence Both Innervated and Non-Innervated Skeletal Myofiber Formation in Custom Mechanobioreactors","authors":"Melanie C. Hilman, Foteini Mourkioti, Suradip Das, D. Kacy Cullen","doi":"10.1002/biot.70047","DOIUrl":"10.1002/biot.70047","url":null,"abstract":"While it is understood that muscle tissue generates contractile forces, it is less appreciated that muscle dynamically responds to applied forces during development. We previously fabricated tissue engineered muscle comprised of skeletal myocytes in co-culture with spinal motor neurons on aligned nanofiber poly-caprolactone scaffolding, demonstrating that innervation elicited more robust myofibers and formation of neuromuscular junctions. The current study utilized custom mechanobioreactors to apply tensile elongation to this engineered muscle platform to explore the effects of exogenous forces and scaffold topology on innervated versus non-innervated myocytes. Nanofiber scaffold alignment played a significant role in myocyte thickness, width, and fusion under both innervated and non-innervated conditions. A combination of tensile loading and nanofiber alignment increased myocyte fusion, suggesting these parameters work together to expedite and enhance myofiber formation and maturation. Overall, this multi-faceted paradigm, featuring biomechanical loading, substrate topology, and innervation, mimics key features of the developmental microenvironment experienced by myocytes in vivo. These findings may facilitate more sophisticated studies on muscle development, function, and responses to trauma while also elucidating principles to support the fabrication of engineered muscle to repair major muscle defects.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Directed Evolution of Transcriptional Factor BreR for Improved Substrate Specificity Toward Deoxycholic Acid and Ursodeoxycholic Acid 转录因子BreR定向进化提高对脱氧胆酸和熊脱氧胆酸的底物特异性

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-09 DOI: 10.1002/biot.70058

Jianping Xu, Jian Zhang, Xiaotong Wang, Yue Hao, Jinghai Wang, Baiyu Su, Jingyan Yin, Zhiguo Wang, Qian Wang, Qingsheng Qi

{"title":"Directed Evolution of Transcriptional Factor BreR for Improved Substrate Specificity Toward Deoxycholic Acid and Ursodeoxycholic Acid","authors":"Jianping Xu, Jian Zhang, Xiaotong Wang, Yue Hao, Jinghai Wang, Baiyu Su, Jingyan Yin, Zhiguo Wang, Qian Wang, Qingsheng Qi","doi":"10.1002/biot.70058","DOIUrl":"10.1002/biot.70058","url":null,"abstract":"<div>\u0000 \u0000 Bile acids are endogenous organic acids in mammals that are crucial for digestion and human health. Diverse bile acids serve distinct roles in physiological processes. Deoxycholic acid (DCA) is acknowledged as a biomarker linked to liver and gallbladder disorders, while ursodeoxycholic acid (UDCA) serves as a therapeutic agent for gallstones and various biliary conditions. In this study, we developed a high-throughput screening apparatus utilizing growth coupling and fluorescence sorting to evolve DCA biosensor BreR, and altered its major substrate-binding site to yield mutant I125P, which significantly enhanced the specificity of its DCA response. Simultaneously, we evolved the BreR mutant in response to UDCA. The introduction of 50 mM UDCA resulted in a 235.7% rise in fluorescence intensity, so offering a novel technique for the expedited detection of UDCA. This work establishes an engineering strategy for reprogramming biosensor specificity and provides a foundational platform for UDCA biosensor deployment.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining Acid Stripping With Photobleaching for Sequential Single-Channel Fluorescent Western Blot 酸剥离与光漂白相结合的顺序单通道荧光Western Blot研究

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-09 DOI: 10.1002/biot.70056

Qiuyu Chen, Wenqing Gan, Dawei Shi, Ruibao Ren, Yan Cui

{"title":"Combining Acid Stripping With Photobleaching for Sequential Single-Channel Fluorescent Western Blot","authors":"Qiuyu Chen, Wenqing Gan, Dawei Shi, Ruibao Ren, Yan Cui","doi":"10.1002/biot.70056","DOIUrl":"10.1002/biot.70056","url":null,"abstract":"<div>\u0000 \u0000 Western blot (WB) is widely used for detecting specific proteins and their posttranslational modifications. The prevailing detection methods rely on either chemiluminescence or fluorescence. Fluorescence offers more stable signals, improves quantitative accuracy, and enables multiplex detection across various channels. Among these, the 800-nm near-infrared channel is preferred for its low background, superior signal-to-noise ratio, and compatibility with the inexpensive blocking agent, non-fat milk. In WB, it is often necessary to detect proteins with similar molecular weights or different forms of the same protein on the same blot. However, an optimal method for sequential probing using only the 800-nm channel has not been reported. Here, we demonstrate that combining 10% acetic acid (AA) stripping with photobleaching effectively eliminates 800-nm fluorophore signals from probed blots, enabling sequential probing within the same channel. The 10% AA solution disrupts antigen-antibody binding without causing protein loss from nitrocellulose membranes, achieving over 90% stripping efficiency for most tested antibodies and reducing potential steric hindrance during reprobing. Additionally, illumination with 800-nm light photobleaches residual signals. This approach provides a solution for sequential fluorescent WB using the optimal channel, delivering enhanced reliability and accuracy compared with chemiluminescent methods. Furthermore, this strategy can be extended to other imaging channels as needed.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the Spectrum: Modified Bacteriocin Nisin as a Trojan Horse Against Gram-Negative Salmonella Typhimurium and Escherichia coli 扩大光谱：改良细菌素Nisin作为抗革兰氏阴性鼠伤寒沙门菌和大肠杆菌的特洛伊木马

IF 3.1 3区生物学

Biotechnology Journal Pub Date : 2025-06-09 DOI: 10.1002/biot.70019

Lucia Lanza, Ruth E. Masias, Miriam C. Chalón, Mauricio Cattaneo, Augusto Bellomio

{"title":"Expanding the Spectrum: Modified Bacteriocin Nisin as a Trojan Horse Against Gram-Negative Salmonella Typhimurium and Escherichia coli","authors":"Lucia Lanza, Ruth E. Masias, Miriam C. Chalón, Mauricio Cattaneo, Augusto Bellomio","doi":"10.1002/biot.70019","DOIUrl":"10.1002/biot.70019","url":null,"abstract":"<div>\u0000 \u0000 Nisin is an antimicrobial peptide widely used as a biopreservative to extend shelf life and control foodborne pathogens, particularly Gram-positive bacteria such as Listeria monocytogenes. However, its efficacy against Gram-negative bacteria is limited due to its inability to penetrate their outer membrane and reach its target, lipid II, in the inner membrane. Agents that alter the outer membrane's permeability can overcome this limitation, enabling nisin to access the periplasm and expand its antimicrobial spectrum. In this study, we aimed to extend nisin's spectrum of action towards Gram-negative bacteria by fusing it with salmochelin, a siderophore produced by Salmonella. Siderophores capture extracellular iron and enter cells through specific receptors. The nisin-salmochelin conjugate was synthesized through an external chemical reaction, yielding a compound with activity against Gram-positive and Gram-negative bacteria. Among the Gram-negative bacteria, notable strains include Salmonella enterica Newport, S. enterica Typhimurium, Escherichia coli O157, E. coli EC3 (uropathogenic isolate), and Providencia rettgeri. This novel conjugate exhibits broad-spectrum activity against foodborne pathogens, significantly enhancing nisin's potential as a food biopreservative and expanding its clinical applications.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0