Indian Journal of Microbiology Research最新文献_第2页

Incidence density rate of multidrug-resistant organism (MDRO) at a tertiary care teaching hospital: A retrospective cross-sectional study 一家三级医疗教学医院的多重耐药菌 (MDRO) 发病率：回顾性横断面研究

Indian Journal of Microbiology Research Pub Date : 2024-07-15 DOI: 10.18231/j.ijmr.2024.020

Rupal Patel, Pinal Joshi, Naimika Patel, Chirag Modi

{"title":"Incidence density rate of multidrug-resistant organism (MDRO) at a tertiary care teaching hospital: A retrospective cross-sectional study","authors":"Rupal Patel, Pinal Joshi, Naimika Patel, Chirag Modi","doi":"10.18231/j.ijmr.2024.020","DOIUrl":"https://doi.org/10.18231/j.ijmr.2024.020","url":null,"abstract":": Multidrug-resistant organisms (MDROs) has become a major public health concern. Rise of MDROs and their negative consequences on patient’s survival and health care costs need the assessment of their burden and implementation of practices to control their emergence and spread. There is a lack of literature mentioning incidence density rates (IDR) of various MDRO infections in Indian hospitals. Therefore, the study was conducted to determine the infection IDR and trend of various MDROs at a tertiary care teaching hospital. It will serve as a benchmark data for similar facilities using the same surveillance definitions and methodology. : A retrospective cross-sectional study was conducted using culture data of ten years (January 2014 to December 2023) at Shree Krishna Hospital, Karamsad. MDRO studied included Methicillin-resistant (MRSA), V (VRE), (CRE), Multidrug-resistant (MDR) spp. and MDR . A total of 7311 MDRO were detected from 1321793 in-patients, showing the overall IDR of 5.53 per 1000 patients-days. CRE (n = 3056, 42%) was the most common MDRO. The overall IDR per 1000 patient-days of MRSA, VRE, CRE, MDR- ., and MDR- was 0.81, 0.23, 2.31, 1.29 and 0.89 respectively. Overall MDRO IDR has increased from 4.20 to 8.77 from 2014 to 2023. An increasing IDR was observed for MRSA (0.57 to 1.48) and MDR- (0.43 to 1.60). Around 80% of the . were MDR as compared to other MDRO. MDROs have been identified as a significant problem at our institute showing an increasing trend. We recommend following stringent infection control practices including isolation protocols as well as adherence to hospital antibiotic policy to reduce the burden of MDROs.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141646127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Outbreak of Mpox - an emerging epidemic and a warning to the world 痘疹的爆发--一种新出现的流行病和对世界的警示

Indian Journal of Microbiology Research Pub Date : 2024-07-15 DOI: 10.18231/j.ijmr.2024.023

Lakshmi Krishnasamy

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Important antiviral properties of Streptomyces species compounds 链霉菌化合物的重要抗病毒特性

Indian Journal of Microbiology Research Pub Date : 2024-05-15 DOI: 10.18231/j.ijmr.2024.002

Ratnesh Kumar, Maneesh Kumar, Suman Kumar, Mithilesh Kumar Jha, Shashank Nand Tiwari, Pratima Gupta

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Genomic perspective of Salmonella enterica isolated from farm animals in Sinaloa, Mexico 从基因组角度看墨西哥锡那罗亚州农场动物中分离出的肠炎沙门氏菌

Indian Journal of Microbiology Research Pub Date : 2024-05-15 DOI: 10.18231/j.ijmr.2024.003

N. C. Campo, J. R. Aguirre-Sánchez, González-López Irvin, Rogelio Prieto-Alvarado, C. Chaidez

{"title":"Genomic perspective of Salmonella enterica isolated from farm animals in Sinaloa, Mexico","authors":"N. C. Campo, J. R. Aguirre-Sánchez, González-López Irvin, Rogelio Prieto-Alvarado, C. Chaidez","doi":"10.18231/j.ijmr.2024.003","DOIUrl":"https://doi.org/10.18231/j.ijmr.2024.003","url":null,"abstract":"Farm animals play a crucial role as a primary source of sustenance for human consumption. However continues to be one of the major food-borne pathogens from a public health standpoint. Its persistent global concern surrounding stems from its capability to induce foodborne illnesses. Unraveling the genetic characteristics of this pathogen stands as a pivotal step, shedding light on the intricate biology of and aiming to mitigate its prevalence. Within this context, our study aims to delve into the genomic traits and population structure of Mexican isolates derived from farm animals. We performed next-generation sequencing across 72 genomes. Genomic data was analyzed to determine virulence and antibiotic resistance markers. In addition, a phylogenetic tree and a spanning tree was constructed. The study revealed a diversity of 18 serovars linked to a singular ST, with prominent serovars being Oranienburg, Give, and Saintpaul. Across all isolates, an extensive array of virulence-related genes was identified. Interestingly, 95% of the isolates displayed exclusive resistance to aminoglycosides, while the remaining exhibited multidrug resistance to tetracycline and chloramphenicol. Notably, a substantial prevalence of prophages in the genomes was observed, accounting for 94% and totaling 183 sequences. The serovar Give stood out with the highest number of sequences, featuring Vibrio X29 and Escher RCS47 as the most prevalent phages. This comprehensive analysis provides valuable insights into the intricate world of , paving the way for enhanced understanding and targeted interventions.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140974479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A study on clinico-demographic and microbiological profile of surgical site infection (SSI) in a tertiary care hospital, Kolkata: An alarming trend 加尔各答一家三级医院手术部位感染 (SSI) 的临床-人口学和微生物学概况研究：令人担忧的趋势

Indian Journal of Microbiology Research Pub Date : 2024-05-15 DOI: 10.18231/j.ijmr.2024.005

Swagata Ganguly Bhattacharjee, Ankita Banik, Sharanya Haldar, Suman Kundu, Somnath Bhunia, Kishor Kumar Behera, Cizarina Roy, J. Dey

{"title":"A study on clinico-demographic and microbiological profile of surgical site infection (SSI) in a tertiary care hospital, Kolkata: An alarming trend","authors":"Swagata Ganguly Bhattacharjee, Ankita Banik, Sharanya Haldar, Suman Kundu, Somnath Bhunia, Kishor Kumar Behera, Cizarina Roy, J. Dey","doi":"10.18231/j.ijmr.2024.005","DOIUrl":"https://doi.org/10.18231/j.ijmr.2024.005","url":null,"abstract":"The Centres for Disease Control and Prevention (CDC) estimates surgical site infections SSI is major contributor of healthcare associated infections (HAI). Multidrug resistant (MDR) Gram-negative bacilli are emerging pathogens. This study aimed to determine the magnitude of SSI and identify predominant pathogens with their antimicrobial susceptibility patterns. It is a hospital-based descriptive cross-sectional study including 2070 suspected SSI specimens from 25809 surgeries between 1July 2021 to 30 June 2023. Organisms’ identification and AST was done by both conventional and automated methods. Data was collected and analysed on MS-Excel sheet with various charts and tables. In our study SSI rate was 6.3%, much higher than previous study (2.83%) from this institution. SSI rate was highest in plastic surgery (8.2%). Major pathogens of SSI were Gram-negative bacilli e.g., (26.34%), (25.59%) and (74.69%) was predominant among Gram-positive cocci. Gram-negative bacilli including enterobacterales and non-fermenter and showed resistance to major classes of broad- spectrum antibiotics. Methicillin resistance (MRSA) was 43.9%, which indicates need to improve infection control practices. Our study showed significant higher proportion of SSI as compared to previous studies from the same institute with alarming number of isolated MDR Gram-negative bacilli. So, this study focusses the need of robust infection control practices and strict implementation of antimicrobial stewardship to overcome challenges of antimicrobial resistance.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140977152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A fatal case of cupriavidus pauculus causing septicemia after duodenal perforation: Case report from university teaching hospital 十二指肠穿孔后引起脓毒血症的致命病例：大学教学医院的病例报告

Indian Journal of Microbiology Research Pub Date : 2024-05-15 DOI: 10.18231/j.ijmr.2024.011

Sheetal Verma, Nishtha Singh, Amber Azim, V. Venkatesh, Armin Ahmad

引用次数: 0

Identification of Trichophyton benhamiae by MALDI-TOF Mass Spectrometry. First report in Peru 通过 MALDI-TOF 质谱法鉴定毛癣菌。秘鲁首次报告

Indian Journal of Microbiology Research Pub Date : 2024-05-15 DOI: 10.18231/j.ijmr.2024.010

Luis Alvarado, M. Quiroz-Reyna, G. Quiroz-Chunga, William Castillo-Aguilar, F. Quedo-Salazar

引用次数: 0

Emerging trends in urinary tract infection pathogens: Insights from K. oxytoca and M. morganii in Indian settings 尿路感染病原体的新趋势：从印度的 K. oxytoca 和 M. morganii 中得到的启示

Indian Journal of Microbiology Research Pub Date : 2024-05-15 DOI: 10.18231/j.ijmr.2024.004

Anju Dhar, Soni Gupta, Deepali Ghoel, Rupesh Dingankar

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Antibiotic stewardship program: challenging threat of antimicrobial resistance 抗生素管理计划：抗菌药耐药性的威胁与挑战

Indian Journal of Microbiology Research Pub Date : 2024-05-15 DOI: 10.18231/j.ijmr.2024.001

Purav G Patel

引用次数: 0

Evaluation of the time, labor, and money required for manual and automated nucleic acid (RNA) isolation for the detection of SARS-COV-2 by QRT-PCR using the qiamp viral RNA mini kit and kingfisher flex 使用 qiamp 病毒 RNA mini 试剂盒和 kingfisher flex 通过 QRT-PCR 检测 SARS-COV-2 所需的人工和自动核酸 (RNA) 分离时间、人力和资金评估

Indian Journal of Microbiology Research Pub Date : 2024-05-15 DOI: 10.18231/j.ijmr.2024.009

Chetana Roat, Nilay Harshadkumar Dave

{"title":"Evaluation of the time, labor, and money required for manual and automated nucleic acid (RNA) isolation for the detection of SARS-COV-2 by QRT-PCR using the qiamp viral RNA mini kit and kingfisher flex","authors":"Chetana Roat, Nilay Harshadkumar Dave","doi":"10.18231/j.ijmr.2024.009","DOIUrl":"https://doi.org/10.18231/j.ijmr.2024.009","url":null,"abstract":"We undertook this cross-sectional investigation to assess the time, manpower, and average run cost per sample using manual Qiamp Viral RNA micro kit (Qiagen) and automated kingfisher flex instrument extraction methods for SARS-Cov-2 identification.The study used 120 Viral Transport Media-collected nasopharyngeal/ oropharyngeal swabs.Magnetic bead-based RNA extraction was performed using the Thermo Fisher Scientific kingfisher flex instrument and manual Extraction was Silica membrane-based Qiagen spin column kits. The TaqPath™ COVID-19 Combo Kit from Thermo Fisher Scientific was used for detecting SARS-CoV-2 target genes.Human technique took 40 minutes longer than automation. It cost more to automate than to manually labor. These disparities in time, effort, and cost affect laboratory operations, offering pros and cons for each method. This suggests that positive or negative was consistent regardless of viral load or RNA concentration.The study found that automated RNA extraction yielded better results compared to manual extraction. The automated sample processing system saved time, people, and money. In resource-limited or low-throughput labs, manual extraction may be preferable. Manual methods are laborious, require more hands-on time, and risk cross-contamination and technical blunders.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140973124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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