In Vitro最新文献_第4页

Modulation of differentiation in vitro. II. Influence of cell spreading and surface events on myogenesis. 体外分化的调节。2细胞扩散和表面事件对肌发生的影响。

In Vitro Pub Date : 1984-09-01 DOI: 10.1007/BF02618874

H Sénéchal, J P Wahrmann, D Delain, A Macieira-Coelho

引用次数: 4

A serum-free culture system for studying solute exchanges in the choroid plexus. 研究脉络膜丛溶质交换的无血清培养系统。

In Vitro Pub Date : 1984-09-01 DOI: 10.1007/BF02618877

W F Agnew, R B Alvarez, T G Yuen, S B Abramson, D Kirk

{"title":"A serum-free culture system for studying solute exchanges in the choroid plexus.","authors":"W F Agnew, R B Alvarez, T G Yuen, S B Abramson, D Kirk","doi":"10.1007/BF02618877","DOIUrl":"https://doi.org/10.1007/BF02618877","url":null,"abstract":"Organ cultures of choroid plexus tissues from the lateral ventricle of juvenile rats have been maintained for periods up to 7 wk in a chemically defined, serum-free media. Of several media and various supplements evaluated, the best growth and survival was obtained with the Pasadena Foundation for Medical Research-4 media supplemented with three hormones: epidermal growth factor, insulin, and hydrocortisone. Autoradiographic studies demonstrated that the epithelial cells incorporated [3H]leucine and [3H]thymidine indicating active protein and DNA synthesis, respectively. The organ cultures were characterized by bulbous, vesicular outgrowths from the choroidal villi explants. The fluid-filled lumina of the vesicles reached diameters of 900 microns and were easily accessed by micropipettes. The walls of the vesicles were composed of single layers of epithelial cells in which the ultrastructural features in the in vivo tissue were well maintained. The in vivo polarity (apical end toward the media and basilar end of the cells toward the luminal cavity) was also maintained. This morphologically stable in vitro system seems to be a promising model for investigation of secretory mechanisms of choroidal tissue.","PeriodicalId":13317,"journal":{"name":"In Vitro","volume":"20 9","pages":"712-22"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02618877","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17557673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparative use of fructose and glucose in human liver and fibroblastic cell cultures. 果糖和葡萄糖在人肝脏和成纤维细胞培养中的比较应用。

In Vitro Pub Date : 1984-09-01 DOI: 10.1007/BF02618875

B Delhotal, F Lemonnier, M Couturier, C Wolfrom, M Gautier, A Lemonnier

{"title":"Comparative use of fructose and glucose in human liver and fibroblastic cell cultures.","authors":"B Delhotal, F Lemonnier, M Couturier, C Wolfrom, M Gautier, A Lemonnier","doi":"10.1007/BF02618875","DOIUrl":"https://doi.org/10.1007/BF02618875","url":null,"abstract":"The effect of fructose as a substitute for glucose in cell culture media was investigated in human skin fibroblast and liver cell cultures. Cells were grown for between 2 and 10 days in identical flasks in four different media, containing 5.5 mmol X 1-1 and 27.5 mmol X 1-1 glucose and fructose, respectively. In the presence of fructose, cell growth was stimulated, but less in liver cells than fibroblasts. At Day 6, increases were observed in [3H]thymidine incorporation, protein levels, and amino acid consumption, and a reduction was noted in ATP levels. In media containing 5.5 mmol X 1-1 glucose or fructose, consumption of fructose was four times lower than that of glucose at Day 3 and did not rise until Day 6. In fructose media, the lactate production was very low (four to five times less than that of glucose) and the pH values were always higher. Some findings were different for the fibroblasts and liver cells, owing to the specific characteristics of these two cell types in culture; this applied especially to the effects of glucose and fructose concentrations of 27.5 mmol X 1-1. Several possible explanations for the stimulation of cell growth in fructose medium were discussed.","PeriodicalId":13317,"journal":{"name":"In Vitro","volume":"20 9","pages":"699-706"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02618875","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17557672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Fish cell lines: establishment and characterization of nine cell lines from salmonids. 鱼类细胞系:九种鲑鱼细胞系的建立和鉴定。

In Vitro Pub Date : 1984-09-01 DOI: 10.1007/BF02618871

C N Lannan, J R Winton, J L Fryer

引用次数: 188

Membrane potentials, electrolyte contents, cell pH, and some enzyme activities of fibroblasts. 膜电位、电解质含量、细胞pH和成纤维细胞的一些酶活性。

In Vitro Pub Date : 1984-09-01 DOI: 10.1007/BF02618872

Y C Yen-Chow, S Y Chow, W S Jee, D M Woodbury

{"title":"Membrane potentials, electrolyte contents, cell pH, and some enzyme activities of fibroblasts.","authors":"Y C Yen-Chow, S Y Chow, W S Jee, D M Woodbury","doi":"10.1007/BF02618872","DOIUrl":"https://doi.org/10.1007/BF02618872","url":null,"abstract":"The resting membrane potential of the cultured fibroblasts derived from rabbit subcutaneous tissues was -10.2 +/- 0.20 mV (n = 390). This potential was affected by the potassium concentration in the culture medium, but not by other chemical or hormonal preparations, such as dibutyryladenosine 3',5'-cyclic monophosphate (0.5 to 5.0 mmol/l), sodium fluoride (10(-5) to 10(-4) M), hydrocortisone (10(-7) to 10(-6) M), parathyroid extract (0.5 to 1.0 U/ml), or thyrotrophin (5 to 10 mU/ml). The Na+, K+, and Cl- concentrations of the cultured fibroblasts were 35.4, 85.7, and 22.6 mmol/l cell water, respectively. The water and protein contents of these cells were 82.1 and 9.18 g/100-g cells, respectively. The intracellular pH of fibroblasts as determined by [14C] dimethyloxazolidine-2, 4-dione, and 3H2O ranged between 6.9 and 7.1 when the pH of the culture medium was maintained at 7.4. The activities of Na+, K+-, HCO3(-)-, and Ca++, Mg++-ATPases in these cultured cells were 19.0 +/- 2.1, 13.6 +/- 2.1, and 6.6 +/- 1.2 nmol pi/mg protein per minute, respectively, and the carbonic anhydrase activity was 0.054 U/mg protein. Calculations based on the values for the membrane potential and the electrolyte concentrations observed in this study indicate that Na+, K+, Cl-, and H+ are not distributed according to their electrochemical gradients across the cell membrane. Na+, Cl-, and H+ are actively transported out of the cells and K+ into the cells.","PeriodicalId":13317,"journal":{"name":"In Vitro","volume":"20 9","pages":"677-84"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02618872","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17302850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Sensitivity of human pancreatic carcinoma cell line (MIA PaCa-2) to Bisantrene and Theprubicin in vitro. 人胰腺癌细胞系(MIA PaCa-2)对双蒽醌和普鲁比星的体外敏感性。

In Vitro Pub Date : 1984-09-01 DOI: 10.1007/BF02618873

G Fountzilas, L O Lim, A A Yunis

引用次数: 1

Cultured hepatoma cells for the study of enzyme regulation: induction of ornithine decarboxylase by insulin and asparagine. 培养肝癌细胞的酶调节研究:胰岛素和天冬酰胺诱导鸟氨酸脱羧酶。

In Vitro Pub Date : 1984-09-01 DOI: 10.1007/BF02618878

V R Potter, T R Evanson, D P Gayda, J A Gurr

引用次数: 1

Changes in serum influence the fatty acid composition of established cell lines. 血清变化影响已建立细胞系的脂肪酸组成。

In Vitro Pub Date : 1984-09-01 DOI: 10.1007/BF02618879

L L Stoll, A A Spector

{"title":"Changes in serum influence the fatty acid composition of established cell lines.","authors":"L L Stoll, A A Spector","doi":"10.1007/BF02618879","DOIUrl":"https://doi.org/10.1007/BF02618879","url":null,"abstract":"The fatty acid composition of different kinds of commercially available serum used to supplement cell culture media differs widely. As compared with fetal bovine serum, horse and bovine calf serum have a very high content of linoleic acid (18:2) and are low in arachidonic acid (20:4). (Fatty acids are abbreviated as number of carbon atoms:number of double bonds). Swine serum contains substantial amounts of both 18:2 and 20:4. Only fetal bovine serum contains more than 1% docosahexaenoic acid (22:6). Considerable differences in fatty acid composition occur when cells are grown in media containing any of these different serum supplements. The 18:2 and 20:4 content of 3T3 mouse fibroblast phospholipids is highest when the medium contains horse serum, intermediate with bovine calf serum, and lowest with swine or fetal bovine serum. Likewise, the highest phospholipid 18:2 content in Madin-Darby canine kidney cells (MDCK) occurs when the medium contains horse serum. With MDCK cells, however, growth in swine serum produces the highest 20:4 content. The 3T3 cell phospholipids accumulate more than 1% 22:6 only when the medium contains fetal bovine serum, whereas in no case do the MDCK cell phospholipids accumulate appreciable amounts of 22:6. The fact that the cellular fatty acid composition is likely to change should be taken into account when changes are contemplated in the serum used to grow established cell lines.","PeriodicalId":13317,"journal":{"name":"In Vitro","volume":"20 9","pages":"732-8"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02618879","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17557674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 64

On disturbance of homeostasis in organ-cultured tissue. 器官培养组织内稳态紊乱的研究。

In Vitro Pub Date : 1984-08-01 DOI: 10.1007/BF02619609

K Norrby, S Bergström, P Druvefors

{"title":"On disturbance of homeostasis in organ-cultured tissue.","authors":"K Norrby, S Bergström, P Druvefors","doi":"10.1007/BF02619609","DOIUrl":"https://doi.org/10.1007/BF02619609","url":null,"abstract":"The intact membranous rat mesentery was cultured in Eagle's minimum essential medium containing no serum or only low concentrations of serum. The procedure is in some important respects superior to previous organ culture techniques. To estimate the extent of disturbance of homeostasis of the tissue in culture, the spontaneous mast-cell histamine release was quantitated after preculture preparation of the specimens and after different intervals in culture. Also, the proliferation of fibroblasts and mesothelial cells that predominate in the mesentery was assessed at 48 h by cytofluorometric quantitation of DNA in single-tissue cells. Spontaneous histamine release was time dependent during cultivation, amounting to ca. 50% at 48 h, and was affected by the medium used for moistening the tissue before cultivation. Culturing also brought about great spontaneous increase in the proliferation of fibroblasts and mesothelial cells, the rate being related to the concentration of serum. Addition of the mast-cell secretagogues 48/80 or polymyxin B at 1 h caused rapid release of 50 to 60% of the histamine and was followed by augmented proliferation in the serum-containing media. The spontaneous increase of cell proliferation in tissue culture may be causally related to mast-cell secretion. Further studies are needed to define factors influencing the spontaneous mast-cell secretion and the mast-cell-dependent mitogenesis in normal tissue cells.","PeriodicalId":13317,"journal":{"name":"In Vitro","volume":"20 8","pages":"607-14"},"PeriodicalIF":0.0,"publicationDate":"1984-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02619609","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17273256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture conditions induce the appearance of immortalized C3H mouse cell lines. 培养条件诱导C3H小鼠永生化细胞系的出现。

In Vitro Pub Date : 1984-08-01 DOI: 10.1007/BF02619607

L Curatolo, E Erba, L Morasca

引用次数: 9