Indian journal of biochemistry & biophysics最新文献_第6页

Lys-413 of S-phase mRNA cycling sequence binding protein from Leishmania donovani (LdCSBP) is modified through monoubiquitination that is responsible for inhibition of its riboendonuclease activity. 多诺瓦利什曼原虫(Leishmania donovani, LdCSBP) s期mRNA循环序列结合蛋白Lys-413通过单泛素化修饰，负责抑制其核糖核酸酶活性。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Kasturi Guha, Sneha Das, Partha Saha

{"title":"Lys-413 of S-phase mRNA cycling sequence binding protein from Leishmania donovani (LdCSBP) is modified through monoubiquitination that is responsible for inhibition of its riboendonuclease activity.","authors":"Kasturi Guha, Sneha Das, Partha Saha","doi":"","DOIUrl":"","url":null,"abstract":"In addition to well-known process of proteasome-mediated degradation of polyubiquitinated proteins, monoubiquitination of proteins is also an important post-translational modification that regulates various non-degradative cellular processes like protein trafficking, cellular signalling, DNA replication and DNA repair. We have previously characterized a multi-domain cycling sequence binding protein LdCSBP from Leishmania donovani, which binds specifically to a conserved CAUAGAAG octamer containing RNAs via its uniquely arranged CCCH type Zn-fingers and degrades them using its Smr endonuclease domain, indicative of its potential role in the turnover of the S-phase mRNAs. Remarkably, its riboendonuclease activity is inhibited due to the incorporation of a monoubiquitin residue in the ZnF domain, though the target Lys residue remains unknown. Here, we report through systematic mutation of Lys residue to Ala that Lys-413 in LdCSBP is the site of monoubiquitination. However, the amino acid motif around the target Lys in LdCSBP is not consensus with any previously known monoubiquitination site, though partial homology is observed with a subset of recently identified mammalian ubiquitination target sites. Interestingly, Lys-413 of LdCSBP is conserved in the homologous annotated proteins from the related kinetoplastida parasites, suggesting similar monoubiquitination-mediated regulation of RNA endonuclease activity in the organisms.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"51 6","pages":"559-66"},"PeriodicalIF":1.4,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33172605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin signaling network in cancer. 癌症中的胰岛素信号网络。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Alpana Ray, Mohamed Alalem, Bimal K Ray

引用次数: 0

Involvement of the early growth response protein 1 in vascular pathophysiology: an overview. 早期生长反应蛋白1在血管病理生理中的参与:综述。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Estelle R Simo Cheyou, Viktoria Youreva, Ashok K Srivastava

{"title":"Involvement of the early growth response protein 1 in vascular pathophysiology: an overview.","authors":"Estelle R Simo Cheyou, Viktoria Youreva, Ashok K Srivastava","doi":"","DOIUrl":"","url":null,"abstract":"Hyperactivation of proliferative and growth promoting pathways underlies the progression of vessel remodeling, leading to vascular dysfunction. An upregulation of early growth response protein 1 (Egr-1), a zinc finger transcription factor has been observed in several models of vascular diseases. In the vasculature, Egr-1 expression can be induced by multiple hormonal, metabolic and external stimuli, such as growth factors, cytokines, reactive oxygen species, hyperglycaemia and stretch-induced stress. The structure of the Egr-1 promoter allows both its auto-regulation and its binding with several regulatory transcription cofactors like the serum response factor and the cAMP response element binding protein. Pharmacological and genetic studies have revealed the involvement of several signaling pathways that contribute to the expression of Egr-1. Among them, the mitogen-activated protein kinase pathway has emerged as a predominant signaling cascade that regulates Egr-1 transcription in response to various stimuli. Moreover, targeted deletion of Egr-1 by DNAzymes, antisense oligonucleotides or RNA interference has also helped in defining the importance of Egr-1 in the pathophysiology of vascular diseases. Neointimal formation and expression of genes directly linked with proinflammatory processes have been demonstrated to be enhanced by Egr-1 expression and activity. This review provides an overview on the signaling components implicated in Egr-1 expression and discusses its potential involvement in vascular pathophysiology.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"51 6","pages":"457-66"},"PeriodicalIF":1.4,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33054218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phospholipid remodeling and eicosanoid signaling in colon cancer cells. 结肠癌细胞中的磷脂重塑和类二十烷信号传导。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Siddhartha Das, Leobarda Robles Martinez, Suparna Ray

引用次数: 0

Challenges of ionizing radiation in tumor treatment and role of angiogenesis. 电离辐射在肿瘤治疗中的挑战和血管生成的作用。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Subir Kumar Das

引用次数: 0

Herbicide paraquat induces sex-specific variation of neuroinflammation and neurodegeneration in Drosophila melanogaster. 除草剂百草枯诱导黑腹果蝇神经炎症和神经变性的性别特异性变异。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Shauryabrota Dalui, Arindam Bhattacharyya

{"title":"Herbicide paraquat induces sex-specific variation of neuroinflammation and neurodegeneration in Drosophila melanogaster.","authors":"Shauryabrota Dalui, Arindam Bhattacharyya","doi":"","DOIUrl":"","url":null,"abstract":"There are several reports on herbicide paraquat (PQ)-induced Parkinsonian-like pathology in different animal models, including Drosophila melanogaster. Also, the role of some inflammatory factors, such as nitric oxide is reported in PQ-induced neuroinflammation of Drosophila. Although invertebrate model is valuable to study the conserved inflammatory pathway at the time of neurodegeneration, but neuroinflammation during PQ-mediated neurodegeneration has not been studied explicitly in Drosophila. In this study, the inflammatory response was examined in Drosophila model during PQ-induced neurodegeneration. We found that after exposure to PQ, survivability and locomotion ability were affected in both sexes of Drosophila. Behavioural symptoms indicated similar physiological features of Parkinson's disease (PD) in different animal models, as well as in humans. Our study revealed alteration in proinflamatory factor, TNF-α and Eiger (the Drosophila homologue in TNF superfamily) was changed in PQ-treated Drosophila both at protein and mRNA level during neurodegeneration. To ensure the occurrence of neurodegeneration, tyrosine hydroxylase (TH) positive neuronal cell loss was considered as a hallmark of PD in the fly brain. Thus, our result revealed the conserved inflammatory events in terms of expression of TNF-α and Eiger present during a sublethal dose of PQ-administered neurodegeneration in male and female Drosophila with significant variation in proinflammatory factor level among both the sexes.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"51 6","pages":"567-73"},"PeriodicalIF":1.4,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33172606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms for the defects in phospholipid signal transduction in diabetic cardiomyopathy. 糖尿病性心肌病中磷脂信号转导缺陷的机制。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Paramjit S Tappia, Naranjan S Dhalla

引用次数: 0

Implications of fundamental signalling alterations in diabetes mellitus-associated cardiovascular disease . 糖尿病相关心血管疾病基本信号改变的意义

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Pitchai Balakumar

引用次数: 0

TLR4-mediated activation of MyD88 signaling induces protective immune response and IL-10 down-regulation in Leishmania donovani infection. tlr4介导的MyD88信号激活诱导多诺瓦利什曼原虫感染的保护性免疫反应和IL-10下调

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Joydeep Paul, Kshudiram Naskar, Sayan Chowdhury, Md Nur Alam, Tapati Chakraborti, Tripti De

{"title":"TLR4-mediated activation of MyD88 signaling induces protective immune response and IL-10 down-regulation in Leishmania donovani infection.","authors":"Joydeep Paul, Kshudiram Naskar, Sayan Chowdhury, Md Nur Alam, Tapati Chakraborti, Tripti De","doi":"","DOIUrl":"","url":null,"abstract":"In visceral leishmaniasis, a fragmentary IL-12 driven type 1 immune response along with the expansion of IL-10 producing T-cells correlates with parasite burden and pathogenesis. Successful immunotherapy involves both suppression of IL-10 production and enhancement of IL-12 and nitric oxide (NO) production. As custodians of the innate immunity, the toll-like receptors (TLRs) constitute the first line of defense against invading pathogens. The TLR-signaling cascade initiated following innate recognition of microbes shapes the adaptive immune response. Whereas numerous studies have correlated parasite control to the adaptive response in Leishmania infection, growing body of evidence suggests that the activation of the innate immune response also plays a pivotal role in disease pathogenicity. In this study, using a TLR4 agonist, a Leishmania donovani (LD) derived 29 kDa β 1,4 galactose terminal glycoprotein (GP29), we demonstrated that the TLR adaptor myeloid differentiation primary response protein-88 (MyD88) was essential for optimal immunity following LD infection. Treatment of LD-infected cells with GP29 stimulated the production of IL-12 and NO while suppressing IL-10 production. Treatment of LD-infected cells with GP29 also induced the degradation of IKB and the nuclear translocation of NF-κB, as well as rapid phosphorylation of p38 MAPK and p54/56 JNK. Knockdown of TLR4 or MYD88 using siRNA showed reduced inflammatory response to GP29 in LD-infected cells. Biochemical inhibition of p38 MAPK, JNK or NF-κB, but not p42/44 ERK, reduced GP29-induced IL-12 and NO production in LD-infected cells. These results suggested a potential role for the TLR4-MyD88-IL-12 pathway to induce adaptive immune responses to LD infection that culminated in an effective control of intracellular parasite replication.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"51 6","pages":"531-41"},"PeriodicalIF":1.4,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33172602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preface. London tap water experiment. 前言。伦敦自来水实验。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2014-12-01

Chakraborti Sajal

引用次数: 0