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SEARCH AND CHARACTERIZATION OF ELECTRONOACEPTORS FOR L-GALACTONE-1,4-LACTONE DEHYDROGENASE FROM ARABIDOPSIS THALIANA 拟南芥中l -半乳糖-1,4-内酯脱氢酶电子受体的寻找与表征
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-96-99
E. Kudryashova
{"title":"SEARCH AND CHARACTERIZATION OF ELECTRONOACEPTORS FOR L-GALACTONE-1,4-LACTONE DEHYDROGENASE FROM ARABIDOPSIS THALIANA","authors":"E. Kudryashova","doi":"10.37747/2312-640x-2021-19-96-99","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-96-99","url":null,"abstract":"Natural (cytochrome C) and artificial electron acceptors (1,4-benzoquinone, phenazine methosulfate, tetramethylphenylenediamine) were characterized, and the possibility of using the acceptors to determine the enzymatic activity of L-galactone-1,4-lactone dehydrogenase of Arabidopsis Thaliana in an aqueous solution and in a micellar system was investigated.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75441401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PREPARATION OF A LIPOSOMAL CONJUGATE OF THE MUTANT EXTRACELLULAR DOMAIN OF THE MOUSE PD-1 RECEPTOR 小鼠pd-1受体胞外结构域突变体脂质体偶联物的制备
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-91-92
D. Ryazantsev, E. Svirshchevskaya, E. Vodovozova
{"title":"PREPARATION OF A LIPOSOMAL CONJUGATE OF THE MUTANT EXTRACELLULAR DOMAIN OF THE MOUSE PD-1 RECEPTOR","authors":"D. Ryazantsev, E. Svirshchevskaya, E. Vodovozova","doi":"10.37747/2312-640x-2021-19-91-92","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-91-92","url":null,"abstract":"Liposomal conjugate of the mutant extracellular domain of the mouse PD-1 receptor obtained to inhibit the PD-1/PD-L1 signaling pathway.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73251817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DESIGN OF A NEW ETHYLENE PLANT HORMONE SENSOR BASED ON GENOME-WIDE DATA ANALYSIS OF EIN3 TRANSCRIPTION FACTOR BINDING 基于ein3转录因子结合全基因组数据分析的新型乙烯植物激素传感器设计
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-199-200
V. Levitsky, D. Oshchepkov, E. Zemlyanskaya
{"title":"DESIGN OF A NEW ETHYLENE PLANT HORMONE SENSOR BASED ON GENOME-WIDE DATA ANALYSIS OF EIN3 TRANSCRIPTION FACTOR BINDING","authors":"V. Levitsky, D. Oshchepkov, E. Zemlyanskaya","doi":"10.37747/2312-640x-2021-19-199-200","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-199-200","url":null,"abstract":"A new structural variant of the transcription factor EIN3 binding site has been obtained. This structural variant is strongly associated with the transcriptional response to ethylene and is preferred for EIN3 binding. Based on this structural variant, a new genetic ethylene sensor was developed.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76008906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ELECTROCHEMICAL ACETYLCHOLINESTERASE SENSORS BASED ON CARBON NANOMAERIALS FOR THE DETERMINATION OF DRUGS AND INSECTICIDES 基于碳纳米材料的电化学乙酰胆碱酯酶传感器用于药物和杀虫剂的测定
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-231-232
R. Shamagsumova
{"title":"ELECTROCHEMICAL ACETYLCHOLINESTERASE SENSORS BASED ON CARBON NANOMAERIALS FOR THE DETERMINATION OF DRUGS AND INSECTICIDES","authors":"R. Shamagsumova","doi":"10.37747/2312-640x-2021-19-231-232","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-231-232","url":null,"abstract":"Operational and analytical characteristics of electrochemical biosensors based on acetylcholinesterase have been considered for the determination of enzyme inhibitors. As was shown, application of the mixtures of carbon nanomaterials increases sensitivity of determination over that of single component coatings. Biosensors were tested on determination of reversible inhibitors in urea and blood serum.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76116807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MODELING REACTION MECHANISM OF GLUTAMATE CARBOXYPEPTIDASE – A MEMBER OF THE POLYENZYME CHAIN OF N-ACETYLASPARTATE 模拟n -乙酰天冬氨酸多酶链成员谷氨酸羧肽酶的反应机理
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-117-118
M. Khrenova, A. V. Krivitskaya
{"title":"MODELING REACTION MECHANISM OF GLUTAMATE CARBOXYPEPTIDASE – A MEMBER OF THE POLYENZYME CHAIN OF N-ACETYLASPARTATE","authors":"M. Khrenova, A. V. Krivitskaya","doi":"10.37747/2312-640x-2021-19-117-118","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-117-118","url":null,"abstract":"Computational methods of molecular modeling are used to simulate the reaction mechanism of hydrolysis of N-acetyl-L-aspartyl-L-glutamate catalyzed by glutamate carboxypeptidase II to N-acetylaspartate and glutamate.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79361922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PLANT-DERIVED VESICLES AS CARRIES OF EXOGENOUS FUNCTIONAL BIOMOLECULES 植物源性囊泡作为外源功能性生物分子的载体
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-59-61
R. Kamyshnsky, E. Varfolomeeva, S. Landa, Y. Kil, E. Komarova, E. Putevich, A. Konevega, T. Shtam
{"title":"PLANT-DERIVED VESICLES AS CARRIES OF EXOGENOUS FUNCTIONAL BIOMOLECULES","authors":"R. Kamyshnsky, E. Varfolomeeva, S. Landa, Y. Kil, E. Komarova, E. Putevich, A. Konevega, T. Shtam","doi":"10.37747/2312-640x-2021-19-59-61","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-59-61","url":null,"abstract":"The efficiency of delivery of exogenous functional biomolecules to human cells using plant-derived vesicles has been demonstrated in vitro. Also the distribution of loaded plant vesicles in the tissues was studied in vivo.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81499858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RUSSIAN SCHOOL-CONFERENCE "ECOTOXICOLOGY" - A KEY ELEMENT FOR ATTRACTING STUDENTS TO SOLVE INNOVATIVE TASKS OF ENVIRONMENTAL PROTECTION BIOTECHNOLOGY 俄罗斯学校会议“生态毒理学”-吸引学生解决环境保护生物技术创新任务的关键因素
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-371-373
V. A. Alferov, K. Osina, A. Boronin
{"title":"RUSSIAN SCHOOL-CONFERENCE \"ECOTOXICOLOGY\" - A KEY ELEMENT FOR ATTRACTING STUDENTS TO SOLVE INNOVATIVE TASKS OF ENVIRONMENTAL PROTECTION BIOTECHNOLOGY","authors":"V. A. Alferov, K. Osina, A. Boronin","doi":"10.37747/2312-640x-2021-19-371-373","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-371-373","url":null,"abstract":"The leading forms of recruiting the gifted youth to solution of innovative tasks are scientific workshops and conferences. The Russian Young Scientist School-Conference “Ecotoxicology” has been held in Tula State University since 2006, and it plays an important role in training biotechnologists in environmental field of research.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"487 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84409811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PREPARATION OF CATALASE CROSS-LINKED ENZYME AGGREGATES ON THE BASE OF VATERITE MATRIX 在水晶石基质上制备过氧化氢酶交联酶聚集体
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-106-108
N. L. Eremeev, N. G. Balabushevich, D. Volodkin, N. Klyachko
{"title":"PREPARATION OF CATALASE CROSS-LINKED ENZYME AGGREGATES ON THE BASE OF VATERITE MATRIX","authors":"N. L. Eremeev, N. G. Balabushevich, D. Volodkin, N. Klyachko","doi":"10.37747/2312-640x-2021-19-106-108","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-106-108","url":null,"abstract":"A procedure has been developed for the synthesis of cross-linking aggregates of catalase by treatment with glutaraldehyde incorporated into the pores of the vaterite matrix and subsequent dissolution of the inorganic core. The resulting aggregates have a spherical shape with a narrow particle size distribution and a high specific activity of the enzyme.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85926317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MAGNETIC MIP TETRACYCLINE NANOPARTICLES – SYNTHESIS AND APPLICATION IN PIEZOELECTRIC SENSORS 磁性四环素纳米颗粒的合成及其在压电传感器中的应用
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-42-44
E. V. Bizina, T. Ermolaeva
{"title":"MAGNETIC MIP TETRACYCLINE NANOPARTICLES – SYNTHESIS AND APPLICATION IN PIEZOELECTRIC SENSORS","authors":"E. V. Bizina, T. Ermolaeva","doi":"10.37747/2312-640x-2021-19-42-44","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-42-44","url":null,"abstract":"Magnetic polymers with tetracycline molecular imprints have been synthesized. The conditions for the formation of a recognition layer of a piezoelectric sensor based on Fe3O4@MIP particles have been studied. It is shown that the use of the developed nanomaterial makes it possible to reduce the time required to prepare the sensor for analysis by 25 times in comparison with traditional methods.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"131 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77092049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
BIOSENSING AND MEDICAL DIAGNOSTICS BASED ON ULTRA SENSITIVE OPTICAL AND MAGNETIC MEASUREMENTS 基于超灵敏光学和磁测量的生物传感和医学诊断
http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-238-240
A. Orlov, V. A. Bragina, N.V. Gyteneva, M. Nikitin, B. G. Gorshkov
{"title":"BIOSENSING AND MEDICAL DIAGNOSTICS BASED ON ULTRA SENSITIVE OPTICAL AND MAGNETIC MEASUREMENTS","authors":"A. Orlov, V. A. Bragina, N.V. Gyteneva, M. Nikitin, B. G. Gorshkov","doi":"10.37747/2312-640x-2021-19-238-240","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-238-240","url":null,"abstract":"Highly sensitive methods of optical and magnetic measurements have been developed and tested for analytical applications, medical diagnostics, food safety control, etc. The achieved detection limits are unique for express assays: DNA - 6 × 10-19 mol (without amplification), haptens - 16 fg/ml, protein biomarkers - 4 pg/ml.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80843978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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