Hoppe-Seyler's Zeitschrift fur physiologische Chemie最新文献

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Endogenous lectins of bovine pancreas. 牛胰腺内源性凝集素。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-06-01 DOI: 10.1515/bchm2.1984.365.1.633
H J Gabius, R Engelhardt, F Cramer
{"title":"Endogenous lectins of bovine pancreas.","authors":"H J Gabius,&nbsp;R Engelhardt,&nbsp;F Cramer","doi":"10.1515/bchm2.1984.365.1.633","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.633","url":null,"abstract":"<p><p>Affinity chromatography of salt and detergent extracts from bovine pancreas on glycosylated or glycoprotein-linked Sepharose 4B resulted in purification of different carbohydrate-binding proteins. Three species of proteins with molecular masses of 16 kDa, 35 kDa and 64 kDa exhibiting specificity for beta-galactosides, but none with preferential specificity for alpha-galactosides, were isolated from salt and detergent extracts. No Ca2+ was required for binding. Mannan-binding proteins of 37 kDa, 47 kDa and 94 kDa without Ca2+-requirement were only found in the salt extract. No other mannan-binding activity could be detected. Fucose-binding proteins of 34 kDa, 62 kDa and 70 kDa exhibiting Ca2+-requirement for binding were present in the salt extract and two proteins with 62 kDa and 70 kDa in detergent extract. The different fractions showed agglutination activity when assayed with rabbit erythrocytes. Thus they can be defined as lectins.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 6","pages":"633-8"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.633","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17536430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Evidence for the association of core histones with poly(U) to nucleosome-like complexes. 核心组蛋白与聚(U)核小体样复合物关联的证据。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-06-01 DOI: 10.1515/bchm2.1984.365.1.661
H H Klump, H Falk
{"title":"Evidence for the association of core histones with poly(U) to nucleosome-like complexes.","authors":"H H Klump,&nbsp;H Falk","doi":"10.1515/bchm2.1984.365.1.661","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.661","url":null,"abstract":"<p><p>As was shown in a recent paper [Klump, H. & Hütig, H. (1980) Ber. Bunsenges. Phys. Chem. 84, 250-253] by one of the authors (H.K.), nucleosome-like histone complexes can be formed on ribopolynucleotides as well as on deoxyribopolynucleotides. The conditions used for complex formation have to be such that single strands and triple strands as secondary structures of the polynucleotides employed are excluded under all ionic-strength conditions. There is clear evidence however for ordered poly(U)/histone complexes. In this paper we further characterize these poly(U) complexes and present electron micrographs of both DNA histone complexes and poly(U) histone complexes to show their close resemblance.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 6","pages":"661-5"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.661","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17536431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunological correspondence between arthropod hemocyanin subunits. I. Scorpion (Leiurus, Androctonus) and spider (Eurypelma, Cupiennius) hemocyanin. 节肢动物血青素亚基之间的免疫对应关系。蝎子(Leiurus, Androctonus)和蜘蛛(Eurypelma, Cupiennius)的血青素。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-06-01 DOI: 10.1515/bchm2.1984.365.1.619
J Markl, W Gebauer, R Runzler, I Avissar
{"title":"Immunological correspondence between arthropod hemocyanin subunits. I. Scorpion (Leiurus, Androctonus) and spider (Eurypelma, Cupiennius) hemocyanin.","authors":"J Markl,&nbsp;W Gebauer,&nbsp;R Runzler,&nbsp;I Avissar","doi":"10.1515/bchm2.1984.365.1.619","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.619","url":null,"abstract":"<p><p>The hemocyanins of the scorpions Leiurus quinquestriatus and Androctonus australis, the tarantula Eurypelma californicum (all 24-mers), and the lycosid spider Cupiennius salei (dodecamer) were dissociated into subunits, the subunits isolated and studied by two-dimensional immunoelectrophoresis for interspecific cross-reactivities. Androctonus hemocyanin yielded a pattern of 8 subunit types in agreement with data from Lamy et al. (1979, Arch. Biochem. Biophys. 193, 140-149). Leiurus hemocyanin is also composed of 8 immunologically distinct subunits which could be assigned to the pattern of Androctonus in a subunit-to-subunit correlation. The subunit designations 1 to 6 of Lamy et al. could be adopted for both scorpion hemocyanins; however, in the present communication, Lamy's subunits 3A/3B are designated as 3'/3\", because we could not unequivocally decide if 3' = 3A and 3\" = 3B or vice versa. The 7 subunit types a to g of Eurypelma hemocyanin could be correlated with the scorpion hemocyanin subunits as follows: a = 3', b = 5B, c = 3C, d = 5A, e = 6, f = 2, g = 4. Additional cross-reactivities were detected between e/4, and f/5A, respectively. No subunit of Eurypelma hemocyanin is homologous to scorpion 3\", which could not be precipitated by anti-Eurypelma antiserum. Antiserum against Cupiennius hemocyanin precipitated subunit f of Eurypelma and subunits 2 and 5A of scorpion hemocyanin. The published models of quaternary structure and a possible subunit phylogeny of arachnidan hemocyanins are discussed in view of the present results.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 6","pages":"619-31"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17536930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Primary structure, biochemical and physiological aspects of hemoglobin from South American lungfish (Lepidosiren paradoxus, Dipnoi). 南美肺鱼血红蛋白的初级结构、生化和生理方面。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-06-01 DOI: 10.1515/bchm2.1984.365.1.639
K Rodewald, A Stangl, G Braunitzer
{"title":"Primary structure, biochemical and physiological aspects of hemoglobin from South American lungfish (Lepidosiren paradoxus, Dipnoi).","authors":"K Rodewald,&nbsp;A Stangl,&nbsp;G Braunitzer","doi":"10.1515/bchm2.1984.365.1.639","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.639","url":null,"abstract":"<p><p>The South American Lungfish has only one hemoglobin component. The complete amino-acid sequence of this hemoglobin is presented. A large quantity of carbonate dehydratase from the lungfish erythrocytes was also isolated. The carboxymethylated chains, obtained by separation of globin on DEAE-Sephacel, were submitted to tryptic digestion and chemical cleavage. The isolation of tryptic peptides was achieved either by Dowex-50 chromatography or by high performance liquid chromatography. The alignment of peptides was performed by homology with the previously established sequences of the carp and goldfish hemoglobins. The overlapping peptides confirmed this sequence. The alpha chains have 143 residues, the beta chains 147. The relation between the primary structure and the physiological properties of lungfish hemoglobin are discussed.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 6","pages":"639-49"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.639","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17157637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Tartrate-inhibitable acid phosphatase. Purification from placenta, characterization and subcellular distribution in fibroblasts. 酒石酸抑制酸性磷酸酶。胎盘的纯化,成纤维细胞的特性和亚细胞分布。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-06-01 DOI: 10.1515/bchm2.1984.365.1.651
V Gieselmann, A Hasilik, K von Figura
{"title":"Tartrate-inhibitable acid phosphatase. Purification from placenta, characterization and subcellular distribution in fibroblasts.","authors":"V Gieselmann,&nbsp;A Hasilik,&nbsp;K von Figura","doi":"10.1515/bchm2.1984.365.1.651","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.651","url":null,"abstract":"<p><p>Tartrate-inhibitable acid phosphatase was purified to apparent homogeneity from human placenta. The enzyme is composed of two subunits with an apparent molecular mass of 48 kDa. Each subunit carries one oligosaccharide of the high-mannose/hybride type. The purified enzyme has an isoelectric point of pH 6.2. It cleaves phosphomonoester bonds at acid pH, is competitively inhibited by L-tartrate, Ki = 0.51 microM, and phosphate, Ki = 0.8mM. A monospecific antiserum raised against the purified placental enzyme precipitated 62% and 85% of the tartrate-inhibitable acid phosphatase present in extracts of placenta and fibroblasts, respectively. By means of subcellular fractionation and immunoprecipitation it was shown that the majority of tartrate-inhibitable acid phosphatase is located in lysosomes in normal and mucolipidosis II fibroblasts. In the human Hep G-2 hepatoma cells a significant fraction of the enzyme appears to be associated with non-lysosomal organelles.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 6","pages":"651-60"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.651","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17157638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
Pterins and the regulation of lymphocyte activation on the mode of xanthopterin action. 黄蝶素与淋巴细胞活化对黄蝶素作用模式的调节。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-06-01 DOI: 10.1515/bchm2.1984.365.1.667
I Ziegler
{"title":"Pterins and the regulation of lymphocyte activation on the mode of xanthopterin action.","authors":"I Ziegler","doi":"10.1515/bchm2.1984.365.1.667","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.667","url":null,"abstract":"<p><p>Some intermediates of pterin anabolism amplify the lectin-induced lymphocyte stimulation while the catabolites xanthopterin and isoxanthopterin terminate their proliferation (Ziegler, I. et al., Cancer Res. 43, 5356 (1983). In the present investigation, we analysed the effect of xanthopterin on total RNA synthesis and on DNA synthesis in both concanavalin A-stimulated lymphocytes and in the lymphoblastoid cell line L 1210. The time courses at various inhibitor concentrations indicated that xanthopterin inhibits RNA synthesis prior to DNA synthesis. Further analysis of the RNA species was performed by double-labeling and subsequent polyacrylamide-gel electrophoresis. Pulse and pulse-chase experiments revealed that an inhibition of 45 S pre-RNA is closer to the target of xanthopterin inhibition than is DNA synthesis.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 6","pages":"667-73"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.667","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17271571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Unexpected occurrence of the Ca 19-9 tumor marker in normal human seminal plasma. 正常人精浆中Ca 19-9肿瘤标志物的意外出现。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-06-01 DOI: 10.1515/bchm2.1984.365.1.613
G Uhlenbruck, U van Meensel-Maene, F G Hanisch, C Dienst
{"title":"Unexpected occurrence of the Ca 19-9 tumor marker in normal human seminal plasma.","authors":"G Uhlenbruck,&nbsp;U van Meensel-Maene,&nbsp;F G Hanisch,&nbsp;C Dienst","doi":"10.1515/bchm2.1984.365.1.613","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.613","url":null,"abstract":"<p><p>A monoclonal antibody with anti-sialyl-lacto-N-fucopentaose II specificity, originally thought to be a specific tumor marker in pancreas- and gastrointestinal tumors, reacts strongly with fucose-rich glycoproteins from human seminal plasma obtained by phenol/saline extraction. They are presumed to come from the prostatic gland secretion. Because of the specificity of this monoclonal antibody it can be deduced that the sialyl-Lea-sequence: N-acetyl-alpha-neuraminyl-(2----3)-beta-D-galactopyranosyl-(1--- -3)-[alpha-L-fucopyranosyl-(1----4)]-2-acetamido-2-deoxy-beta-D-gluco pyranosyl-(1----3)-D-galactopyranosyl is present on these glycoproteins. This is also supported by our carbohydrate analysis.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 6","pages":"613-7"},"PeriodicalIF":0.0,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.613","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17648762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Isolation and amino-acid sequence determination of monkey insulin and proinsulin. 猴胰岛素和胰岛素原的分离及氨基酸序列测定。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-05-01 DOI: 10.1515/bchm2.1984.365.1.571
V K Naithani, G J Steffens, H S Tager, G Buse, A H Rubenstein, D F Steiner
{"title":"Isolation and amino-acid sequence determination of monkey insulin and proinsulin.","authors":"V K Naithani,&nbsp;G J Steffens,&nbsp;H S Tager,&nbsp;G Buse,&nbsp;A H Rubenstein,&nbsp;D F Steiner","doi":"10.1515/bchm2.1984.365.1.571","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.571","url":null,"abstract":"<p><p>Insulin has been isolated and purified from rhesus monkey pancreas by means of acid-ethanol extraction, gel filtration and ion exchange chromatography. The complete amino-acid sequence of the hormone has been determined by amino-acid analysis of the oxidized A- and B-chains, by end group determination, by the identification of the C-terminal residues (AsnA21 and ThrB30) by carboxypeptidase A digestion and by Edman degradation of the S-carboxymethylated A- and B-chains. The 51-residue monkey insulin was shown to be identical to human insulin. From the known insulin and C-peptide sequence the primary sequence of monkey proinsulin has been proposed.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 5","pages":"571-5"},"PeriodicalIF":0.0,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.571","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17441403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Calcium binding to the "stone protein" isolated from pancreatic stones of patients with chronic calcified pancreatitis. 慢性钙化胰腺炎患者胰腺结石中分离的“结石蛋白”与钙的结合。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-05-01 DOI: 10.1515/bchm2.1984.365.1.549
J Lohse, R Kraemer
{"title":"Calcium binding to the \"stone protein\" isolated from pancreatic stones of patients with chronic calcified pancreatitis.","authors":"J Lohse,&nbsp;R Kraemer","doi":"10.1515/bchm2.1984.365.1.549","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.549","url":null,"abstract":"<p><p>Calcium binding to a new secretory protein present in all layers of human pancreatic stones has been studied. This protein with a molecular mass of 13,500 Da has about 4 equivalent binding sites for calcium. Maximum calcium binding was independent of pH and temperature, but the affinity of calcium to this protein increased with decreasing pH and increasing temperature, revealing maximum affinity at pH 7.3 and 37 degrees C. It is concluded that the calcium affinity could explain the presence of the protein in all layers of human pancreatic stones.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 5","pages":"549-54"},"PeriodicalIF":0.0,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.549","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
The primary structure of the hemoglobin of the mole rat (Spalax ehrenbergi, rodentia, chromosome species 60). 鼹鼠血红蛋白的初级结构(Spalax ehrenbergi,啮齿目,染色体种类60)。
Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-05-01 DOI: 10.1515/bchm2.1984.365.1.531
T Kleinschmidt, E Nevo, G Braunitzer
{"title":"The primary structure of the hemoglobin of the mole rat (Spalax ehrenbergi, rodentia, chromosome species 60).","authors":"T Kleinschmidt,&nbsp;E Nevo,&nbsp;G Braunitzer","doi":"10.1515/bchm2.1984.365.1.531","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.1.531","url":null,"abstract":"<p><p>Mole rat (Spalax ehrenbergi) hemoglobin consists of only one component. The complete amino-acid sequence of the alpha- and beta-chains of the species with the diploid chromosome number of 60 is presented. Following chain separation by chromatography on carboxymethyl cellulose CM-52, the primary structures were established by automatic Edman degradation on the chains, on the tryptic peptides, and on a peptide obtained by acid hydrolysis of the Asp-Pro bond in beta-chains. The alignment of the peptides was performed by homology with human alpha- and beta-chains. The comparison showed an exchange of 23 residues in the alpha-chains and 26 in the beta-chains. One substitution in the beta-chains concerns the surrounding of the heme. We found two exchanges in each chain in the alpha 1 beta 1-subunit interface and one in the beta-chain alpha 1 beta 2-contact points. Though all binding sites for 2,3-bisphosphoglycerate are unchanged, the mole rat blood has a high oxygen affinity as a part of adaptation to subterranean life under hypoxia and hypercapnia. A comparison of the sequence with known X-ray models of hemoglobins may give an interpretation of this fact. The primary structure of the mole rat hemoglobin shows more similarities with surface rodents, than with the mole, another small mammal, adapted to hypoxia in subterranean tunnels. The adaptation to hypoxia in mole rat and mole must be due to different mechanisms.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 5","pages":"531-7"},"PeriodicalIF":0.0,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.1.531","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17527019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
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