Hoppe-Seyler's Zeitschrift fur physiologische Chemie最新文献_第6页

Nicotinic acid metabolism. Dimethylmaleate hydratase. 烟酸代谢。Dimethylmaleate水合酶。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-08-01 DOI: 10.1515/bchm2.1984.365.2.847

A Kollmann-Koch, H Eggerer

{"title":"Nicotinic acid metabolism. Dimethylmaleate hydratase.","authors":"A Kollmann-Koch, H Eggerer","doi":"10.1515/bchm2.1984.365.2.847","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.2.847","url":null,"abstract":"The partial enrichment of a new enzyme, dimethylmaleate hydratase from Clostridium barkeri and some of its characteristics are described. The unstable and oxygen-sensitive hydratase depends on ferrous ions and is induced during growth of C. barkeri on nicotinic acid. The enzyme uses both dimethylmaleate and the hydration product, 2,3-dimethylmalate, as substrates to establish an equilibrium that is 70% in favour of the latter acid; dimethylfumarate is not attacked. A 2,3-dimethyl[3-3H]malate specimen was prepared from dimethylmaleate with the hydratase in tritiated water. Based on proton attack at the re-face of the double bond, experimental results indicate the (2R,3S)-configuration for this malate. The hydration reaction takes an anti-course. The tritium label was lost in the sequence (2R,3S)-2,3-dimethyl[3-3H]malate----(R)-[2-3H1]-propionate----(2R) - [2-3H1]propionyl-CoA----(2S)-methylmalonyl-CoA. This result confirms the stereochemical course of the 2,3-dimethylmalate lyase reaction, inversion of configuration, by an independent approach. The hydratase reaction completes the degradation scheme of nicotinic acid by C. barkeri. The pathway is briefly reviewed.","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 8","pages":"847-57"},"PeriodicalIF":0.0,"publicationDate":"1984-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17547206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Measurement of urinary 7,8-dihydro-6-hydroxylumazine in healthy and in Ehrlich ascites tumour-bearing mice. 健康小鼠和埃利希腹水荷瘤小鼠尿中7,8-二氢-6-羟脲嘧啶的测定。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-08-01 DOI: 10.1515/bchm2.1984.365.2.895

H Lutz, D Fuchs, L Fuith, A Hausen, G Reibnegger, H Wachter

引用次数: 4

Processing and transport of lysosomal enzymes in human monocyte line U937. 人单核细胞系U937溶酶体酶的加工和转运。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-08-01 DOI: 10.1515/bchm2.1984.365.2.867

D K Gupta, A Schmidt, K von Figura, A Hasilik

{"title":"Processing and transport of lysosomal enzymes in human monocyte line U937.","authors":"D K Gupta, A Schmidt, K von Figura, A Hasilik","doi":"10.1515/bchm2.1984.365.2.867","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.2.867","url":null,"abstract":"Precursors of cathepsin D and beta-hexosaminidase synthesized in the U937 monocyte line are processed to mature forms with similar kinetics as in fibroblasts. In U937 cells the processing of the precursor of the beta-chain of beta-hexosaminidase, however, results in a larger fragment that resembles a processing intermediate in fibroblasts. This difference is explained by differences in the equipment of the cells with proteinases, since cross-feeding of the precursors to the cells results in a processing characteristic for the recipient cell type. In sucrose gradients the precursors are found partly in a low- and partly in a high-density region. Mature polypeptides and activity of lysosomal enzymes fractionate mainly in the higher density region. In U937 cells the transport and maturation of endogenous lysosomal enzymes are less sensitive to bases (NH4Cl, chloroquine, tilorone) and to antibody against the mannose 6-phosphate specific receptors than in fibroblasts. A small portion of enzymes released from U937 cells contains the markers recognized by the mannose-6-phosphate specific receptors. U937 cells express these receptors and utilize them for transport of endogenous and exogenous lysosomal enzymes. It appears, however, that a fraction of lysosomal enzymes is transported in U937 cells independent of the mannose-6-phosphate-specific receptors.","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 8","pages":"867-76"},"PeriodicalIF":0.0,"publicationDate":"1984-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.867","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17301676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

The light-harvesting polypeptides of Rhodopseudomonas sphaeroides R-26.1. II. Conformational analyses by attenuated total reflection infrared spectroscopy and the possible molecular structure of the hydrophobic domain of the B 850 complex. 球形红假单胞菌R-26.1的捕光多肽。2衰减全反射红外光谱构象分析及b850配合物疏水结构域可能的分子结构。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-07-01

R Theiler, H Zuber

引用次数: 0

Characterization of fibronectin on human spermatozoa. 人精子纤维连接蛋白的特性。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-07-01 DOI: 10.1515/bchm2.1984.365.2.757

M Vuento, P Kuusela, M Virkki, A Koskimies

引用次数: 31

On the role of the pancreatic secretory trypsin inhibitor as an inactivator of trypsin-alpha 2-macroglobulin complexes in acute pancreatitis. 胰腺分泌性胰蛋白酶抑制剂作为胰蛋白酶- α 2巨球蛋白复合物失活剂在急性胰腺炎中的作用。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-07-01 DOI: 10.1515/bchm2.1984.365.2.751

G Balldin, A Borgström, W H Marks, K Ohlsson

{"title":"On the role of the pancreatic secretory trypsin inhibitor as an inactivator of trypsin-alpha 2-macroglobulin complexes in acute pancreatitis.","authors":"G Balldin, A Borgström, W H Marks, K Ohlsson","doi":"10.1515/bchm2.1984.365.2.751","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.2.751","url":null,"abstract":"High levels of immunoreactive pancreatic secretory trypsin inhibitor (PSTI) were demonstrated in the serum and peritoneal exudates of patients suffering from acute pancreatitis. Trypsin-like immunoreactivity in these fluids was found in complex with alpha 1-antitrypsin and in complex with alpha 2-macroglobulin and also as a free peak correlating to free trypsin(ogen). No trypsin-PSTI complexes or PSTI were demonstrated in the macroglobulin fraction of the peritoneal exudates. Saturated and partially saturated trypsin-alpha 2-macroglobulin complexes were prepared in vitro. PSTI was able to partially inhibit the BzArgNan-cleaving activity of both types of complexes in a slow dose-dependent non-linear reaction. Equilibrium was reached in each case within 1 h, but total inhibition was not reached even with large amounts of PSTI. Partially saturated trypsin-alpha 2-macroglobulin complexes were inhibited more readily than saturated complexes. The results support the concept of PSTI acting as a strictly local inhibitor of trypsin in compartments lacking plasma protease inhibitors.","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 7","pages":"751-6"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17271572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The light-harvesting polypeptides of Rhodospirillum rubrum. I. The amino-acid sequence of the second light-harvestng polypeptide B 880-beta (B 870-beta) of Rhodospirillum rubrum S 1 and the carotenoidless mutant G-9+. carotenoidless mutant G-9+. 红红螺旋菌的捕光多肽。1 .红红螺旋藻s1和无类胡萝卜素突变体G-9+第二光集多肽B 880- β (B 870- β)的氨基酸序列。无类胡萝卜素突变体G-9+。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-07-01 DOI: 10.1515/bchm2.1984.365.2.675

R A Brunisholz, F Suter, H Zuber

{"title":"The light-harvesting polypeptides of Rhodospirillum rubrum. I. The amino-acid sequence of the second light-harvestng polypeptide B 880-beta (B 870-beta) of Rhodospirillum rubrum S 1 and the carotenoidless mutant G-9+. carotenoidless mutant G-9+.","authors":"R A Brunisholz, F Suter, H Zuber","doi":"10.1515/bchm2.1984.365.2.675","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.2.675","url":null,"abstract":"The light-harvesting complex B 880 from Rhodospirillum rubrum S 1 (wild type) and B 870 from the carotenoidless mutant G-9+ was shown to consist mainly of an organic solvent-(chloroform/methanol-) soluble and an organic solvent-insoluble polypeptide. The isolation and separation of these two low-molecular-mass polypeptides (Mr 6101 and Mr 6079) were achieved by a two-step extraction procedure of chromatophores using in the first step chloroform/methanol containing 0.1M ammonium acetate. Following Sephadex LH-60 chromatography of this first extract a light-harvesting polypeptide (B 870-alpha) was isolated and its complete amino acid sequence was determined (R. Brunisholz et al. (1981) FEBS Lett. 129/1, 150-154, B 880-alpha: G. Gogel et al. (1983) Biochim. Biophys. Acta 746, 32-39). Upon reextraction of the chromatophore pellet with chloroform/methanol/ammonium acetate containing in addition acetic acid a second low-molecular-mass polypeptide (B 880-beta of B 870-beta) was generated. The complete amino acid sequences of the chloroform/methanol-insoluble light-harvesting polypeptide of Rs. rubrum S 1 (B 880-beta) and of Rs. rubrum G-9+ (B 870-beta) were determined. They are identical and consist of 54 amino acid residues. The conserved histidine residue within the hydrophobic stretch raises more evidence for ligand complexation of bacteriochlorophyll to this specific histidine residue which therefore possibly plays the key role in pigment-protein interactions. Both polypeptides (B 880-alpha and B 880-beta) are part of the light-harvesting complex B 880 in an apparent ratio of 1:1. Based on the primary structure data a possible arrangement of both light-harvesting polypeptides within the membrane will be discussed.","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 7","pages":"675-88"},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.675","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17493452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 75

Demonstration of a new acrosin inhibitor in human seminal plasma. 在人精浆中发现一种新的交叉酶抑制剂。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-07-01 DOI: 10.1515/bchm2.1984.365.2.819

M L Huhtala

引用次数: 33

Direct effect of androgens on progesterone binding and metabolism in rat testis microsomes. 雄激素对大鼠睾丸微粒体孕酮结合及代谢的直接影响。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-07-01 DOI: 10.1515/bchm2.1984.365.2.773

N Kühn-Velten, T Bunse, N Schürer, W Staib

引用次数: 7

Phosphate-haemoglobin interaction. The primary structure of the haemoglobin of the African elephant (Loxodonta africana, Proboscidea): asparagine in position 2 of the beta-chain. Phosphate-haemoglobin交互。非洲象(Loxodonta africana, Proboscidea)血红蛋白的初级结构:-链第2位的天冬酰胺。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-07-01 DOI: 10.1515/bchm2.1984.365.2.743

G Braunitzer, A Stangl, B Schrank, C Krombach, H Wiesner

引用次数: 6