Hoppe-Seyler's Zeitschrift fur physiologische Chemie最新文献_第4页

A one-step liquid-chromatographic technique for the estimation of the deoxythymidine-5'-triphosphatase in human serum. 一步液相色谱法测定人血清中脱氧胸苷- 5′-三磷酸酶。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-10-01 DOI: 10.1515/bchm2.1984.365.2.1263

N Dahlmann

引用次数: 1

Circadian rhythm of acid phosphatase activity in rat liver microsomes and dependence of NADH 5 alpha-reductase on phosphatase activity. 大鼠肝微粒体酸性磷酸酶活性的昼夜节律及NADH 5 α -还原酶对磷酸酶活性的依赖性。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-10-01 DOI: 10.1515/bchm2.1984.365.2.1271

V Graef, S W Golf

引用次数: 2

Purification, characterization and sequence determination of a double-headed trypsin inhibitor peptide from Trichosanthes kirilowii (a Chinese medical herb). 栝楼双头胰蛋白酶抑制剂肽的纯化、鉴定及序列测定。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-10-01 DOI: 10.1515/bchm2.1984.365.2.1211

F L Tan, G D Zhang, J F Mu, N Q Lin, C W Chi

{"title":"Purification, characterization and sequence determination of a double-headed trypsin inhibitor peptide from Trichosanthes kirilowii (a Chinese medical herb).","authors":"F L Tan, G D Zhang, J F Mu, N Q Lin, C W Chi","doi":"10.1515/bchm2.1984.365.2.1211","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.2.1211","url":null,"abstract":"A double-headed trypsin inhibitor peptide was isolated and purified from the root of Trichosanthes kirilowii Maxim (Cucurbitaceae), a Chinese medical herb, by 2.5% trichloroacetic acid and heat treatment followed by affinity chromatography with immobilized trypsin and ion-exchange chromatography. This inhibitor, consisting of 41 amino-acid residues with three pairs of disulfide bonds was sequenced. Two active domains were found to be located at two disulfide loops composed of eight (Pos. 17-24) and nine (Pos. 29-37) amino-acid residues, respectively. It inhibits two molecules of trypsin simultaneously and might be regarded as the smallest double-headed trypsin inhibitor (Mr = 4575) so far known. The chemical modification of the inhibitor with cyclohexandione and citraconic anhydride showed that Arg20-Gly21 and Lys30-Leu31 corresponded to the two reactive sites, respectively. The discovery of the Trichosanthes inhibitor is of importance not only for the study on the structure-function relationship of proteinase inhibitor peptides but also for the search for low molecular mass inhibitors of clinical value among Chinese medical herbs.","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 10","pages":"1211-7"},"PeriodicalIF":0.0,"publicationDate":"1984-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.1211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17500748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Identification of a disialoganglioside (GD1a) containing terminal N-acetyl-9-O-acetylneuraminic acid in rat erythrocytes. 大鼠红细胞中含有末端n -乙酰-9- o -乙酰神经氨酸的双胞脂苷(GD1a)的鉴定。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-10-01 DOI: 10.1515/bchm2.1984.365.2.1247

D C Gowda, G Reuter, A K Shukla, R Schauer

引用次数: 26

Characterization of basic proteins of bull seminal plasma. 公牛精浆碱性蛋白的研究。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-10-01 DOI: 10.1515/bchm2.1984.365.2.1173

M Kemme, R Theil, M V Madiraju, S Scheit, K H Scheit

{"title":"Characterization of basic proteins of bull seminal plasma.","authors":"M Kemme, R Theil, M V Madiraju, S Scheit, K H Scheit","doi":"10.1515/bchm2.1984.365.2.1173","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.2.1173","url":null,"abstract":"We have employed high-performance liquid chromatography on reversed phase columns to analyse the major basic proteins from bull seminal plasma. The proteins were separated preparatively and characterized with respect to molecular mass, amino-acid composition as well as by means of immunodiffusion against specific antisera. The following proteins could be identified: bull seminal proteinase inhibitor II (BUSI II), two seminal RNAases, the seminal antimicrobial protein and proteolytic fragments, derived from it, and a hitherto unknown protein P6 of molecular mass 20 000 Da. Another unknown protein, P5, found to be formed during preparation of the basic protein fraction turned out to be a proteolytic fragment of protein P6 with a molecular mass of 8 750 Da for the polypeptide chain. Antisera against the isolated proteins were raised in rabbits and their specificity established. Single radial immunodiffusion was used to determine the concentration of the above basic proteins in bull seminal plasma: BUSI II (0.25 mg/ml), seminal RNAases (6.5 mg/ml) and protein P6 (2.9 mg/ml).","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 10","pages":"1173-81"},"PeriodicalIF":0.0,"publicationDate":"1984-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.1173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17500746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Extracellular lumazine from aggregating Dictyostelium discoideum cells. Influence of pH on its fluorescence. 聚集盘状网柱体细胞的胞外发光素。pH对其荧光的影响。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-10-01 DOI: 10.1515/bchm2.1984.365.2.1255

I Tatischeff, R Klein

引用次数: 15

[Hemoglobins of reptiles. Expression of alpha-D-genes in the turtles, Chrysemys picta bellii and Phrynops hilarii (Testudines)]. 爬行动物的血红蛋白。α - d基因在龟、赤蠵龟(Chrysemys picta bellii)和黄蠵龟(Phrynops hilarii)中的表达[j]。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-10-01 DOI: 10.1515/bchm2.1984.365.2.1163

K P Rücknagel, E Reischl, G Braunitzer

引用次数: 16

Effect of neutrophil cathepsin G on elastin degradation by neutrophil elastase. 中性粒细胞组织蛋白酶G对中性粒细胞弹性蛋白酶降解弹性蛋白的影响。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-09-01 DOI: 10.1515/bchm2.1984.365.2.1131

C F Reilly, Y Fukunaga, J C Powers, J Travis

引用次数: 32

Polyamine requirement for microbial protein synthesis: structural specificity in cell-free systems of Escherichia coli. 微生物蛋白质合成对多胺的需求:大肠杆菌无细胞系统的结构特异性。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-09-01 DOI: 10.1515/bchm2.1984.365.2.1155

R Praisler, E Männlein, H J Aschhoff, M Mach, W Kersten

{"title":"Polyamine requirement for microbial protein synthesis: structural specificity in cell-free systems of Escherichia coli.","authors":"R Praisler, E Männlein, H J Aschhoff, M Mach, W Kersten","doi":"10.1515/bchm2.1984.365.2.1155","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.2.1155","url":null,"abstract":"Cell-free protein synthesis was performed with synthetic or natural mRNA in an E. coli system containing physiological concentrations of Ca2, Mg2 and either one or both of the two natural polyamines of E. coli, spermidine and putrescine, or corresponding homologues. Putrescine does not permit poly(U)-dependent poly(Phe) synthesis unless spermidine or nor-spermidine is added. Spermidine supports homopeptide synthesis sufficiently well, its effect being stimulated by putrescine or homologous diamines with increasing chain length from 4 to 7 carbon atoms. Diaminopropane completely inhibits the spermidine-activated system in a competitive manner. Translation of MS2 phage RNA is supported by putrescine, the rate and quality (read through to the termination signal) of translation is optimized by spermidine or triamine homologues. MS2 phage RNA translation is supported by spermidine, putrescine has no further stimulatory effect but diaminoheptane enhances the rate of translation. In this case, however, premature chain termination does occur. The results indicate that spermidine is necessary for optimal poly(U) and MS2 phage RNA translation, that the aminopropyl moiety is important for its function and that the remaining side chain can be extended from C4 to C8. Putrescine may cooperate with spermidine but its chain length is rather critical, it cannot substitute for spermidine. The results indicate that the polyamines facilitate mRNA/tRNA/ribosome interactions in a specific manner.","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 9","pages":"1155-62"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.1155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17449547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Measurement of the rat urinary plasminogen activator (esterase A) by direct radioimmunoassay in urine and tissue. 尿和组织中大鼠尿纤溶酶原激活物(酯酶A)的直接放射免疫测定。

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-09-01 DOI: 10.1515/bchm2.1984.365.2.1137

J Chao, K Shimamoto, H S Margolius

{"title":"Measurement of the rat urinary plasminogen activator (esterase A) by direct radioimmunoassay in urine and tissue.","authors":"J Chao, K Shimamoto, H S Margolius","doi":"10.1515/bchm2.1984.365.2.1137","DOIUrl":"https://doi.org/10.1515/bchm2.1984.365.2.1137","url":null,"abstract":"Rat urinary esterase A, a plasminogen activator with kininogenase activity, was recently purified and characterized (J. Chao (1983) J. Biol. Chem. 258, 4434-4439). A sensitive radioimmunoassay for esterase A has been developed. This assay uses a rabbit antiserum in a final dilution of 1:160 000 and the purified enzyme was labelled with 125I using a lactoperoxidase method. It detects 80 pg of immunoreactive material per tube. This antiserum has some cross-reactivity with rat urinary kallikrein (approximately 5%) but a previously characterized tissue kallikrein antiserum has negligible cross-reactivity with the urinary esterase A in the assays. Therefore, kallikrein levels are measured simultaneously in all samples to obtain accurate levels of immunoreactive esterase A. Dilutions of urine or tissue homogenates showed complete parallelism with esterase A standard curves. No cross-reactivity with dog, human or monkey urine was seen. The recovery of esterase A from rat urine was 99.7 +/- 3.5%. Intra- and between-assay errors were 6.5 and 11.2%, respectively. Immunoreactive esterase A was measured and compared with kallikrein levels in rat urine, kidney, pancreas, submandibular gland, descending colon and ileum. The urinary esterase A excretion rate was reduced significantly in rats on a high sodium, compared with a low sodium diet, but not significantly increased above control by the latter. Nonetheless, a significant correlation between urinary kallikrein and esterase A excretion rate was present. This radioimmunoassay can now be used to measure esterase A levels in urine and tissue as questions have arisen about its regulation and functional significance.","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 9","pages":"1137-44"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.1137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17625215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9