{"title":"一步液相色谱法测定人血清中脱氧胸苷- 5′-三磷酸酶。","authors":"N Dahlmann","doi":"10.1515/bchm2.1984.365.2.1263","DOIUrl":null,"url":null,"abstract":"<p><p>Deoxythymidine-5'-triphosphatase (dTTPase) from human serum was separated by DEAE-cellulose chromatography from unspecific hydrolases in a single step. The method was adapted to a microscale and the enzymatic activity was determined for five different groups of patients including epithelial carcinoma, leukemia and three control groups. The result is that the purified dTTPase preparations of these groups reflect the situation obtained in the whole serum.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 10","pages":"1263-9"},"PeriodicalIF":0.0000,"publicationDate":"1984-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.1263","citationCount":"1","resultStr":"{\"title\":\"A one-step liquid-chromatographic technique for the estimation of the deoxythymidine-5'-triphosphatase in human serum.\",\"authors\":\"N Dahlmann\",\"doi\":\"10.1515/bchm2.1984.365.2.1263\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Deoxythymidine-5'-triphosphatase (dTTPase) from human serum was separated by DEAE-cellulose chromatography from unspecific hydrolases in a single step. The method was adapted to a microscale and the enzymatic activity was determined for five different groups of patients including epithelial carcinoma, leukemia and three control groups. The result is that the purified dTTPase preparations of these groups reflect the situation obtained in the whole serum.</p>\",\"PeriodicalId\":13015,\"journal\":{\"name\":\"Hoppe-Seyler's Zeitschrift fur physiologische Chemie\",\"volume\":\"365 10\",\"pages\":\"1263-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.1263\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hoppe-Seyler's Zeitschrift fur physiologische Chemie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/bchm2.1984.365.2.1263\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/bchm2.1984.365.2.1263","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A one-step liquid-chromatographic technique for the estimation of the deoxythymidine-5'-triphosphatase in human serum.
Deoxythymidine-5'-triphosphatase (dTTPase) from human serum was separated by DEAE-cellulose chromatography from unspecific hydrolases in a single step. The method was adapted to a microscale and the enzymatic activity was determined for five different groups of patients including epithelial carcinoma, leukemia and three control groups. The result is that the purified dTTPase preparations of these groups reflect the situation obtained in the whole serum.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
