HemaSphere最新文献

{"title":"Results of the prospective EORTC Children Leukemia Group study 58081 in precursor B- and T-cell acute lymphoblastic leukemia","authors":"Carine Domenech,&nbsp;Michal Kicinski,&nbsp;Barbara De Moerloose,&nbsp;Caroline Piette,&nbsp;Wadih A. Chahla,&nbsp;Laure Kornreich,&nbsp;Marlène Pasquet,&nbsp;Anne Uyttebroeck,&nbsp;Alexandre Theron,&nbsp;Marilyne Poirée,&nbsp;Chloé Arfeuille,&nbsp;Marleen Bakkus,&nbsp;Nathalie Grardel,&nbsp;Catherine Paillard,&nbsp;Claire Freycon,&nbsp;Frédéric Millot,&nbsp;Pauline Simon,&nbsp;Pierre Philippet,&nbsp;Claire Pluchart,&nbsp;Stefan Suciu,&nbsp;Pierre Rohrlich,&nbsp;Alina Ferster,&nbsp;Yves Bertrand,&nbsp;Hélène Cavé,&nbsp;for the Children's Leukemia Group (CLG) of the European Organization for Research and Treatment of Cancer (EORTC)","doi":"10.1002/hem3.70025","DOIUrl":"10.1002/hem3.70025","url":null,"abstract":"<p>Here, we report the results of the prospective cohort study EORTC-CLG 58081 and compare them to the control arm of the randomized phase 3 trial EORTC-CLG 58951, on which treatment recommendations were built. In both studies, patients aged 1–18 years with <i>BCR::ABL1</i> negative acute lymphoblastic leukemia of the B-lineage (B-ALL) or T-lineage (T-ALL) were treated using a BFM backbone without cranial irradiation. Similarly to the control arm of 58951, prednisolone (PRED) 60 mg/m²/day was used for induction therapy, but a few modifications were made. Dexamethasone (DXM) was used in average-risk 2 (AR2) T-ALL and B-ALL during induction, 10 and 6 mg/m²/day, respectively. Leucovorin rescue was delayed to 42 h instead of 36 h after initiation of high-dose methotrexate, and a postconsolidation MRD time point was added to stratify patients. Between 2011 and 2017, 835 patients were prospectively enrolled in the 58081 study. Overall, the 5-year event-free survival (EFS) was 84.8% versus 83.6% (hazard ratio [HR], 0.96 [95% confidence interval [CI]: 0.76–1.21]) for 58081 versus 58951 considered as a control group, respectively, 84.3% versus 84.9% (HR, 1.06 [99% CI: 0.75–1.49]) in B-ALL but 87.3% versus 76.6% (HR, 0.59 [99% CI: 0.28–1.24]) in T-ALL. The comparison between the two studies regarding EFS differed by risk group (<i>p</i> = 0.012). The HR was 2.15 (99% CI: 0.67–6.85) for very low-risk but 0.34 (99% CI: 0.13–0.89) for AR2. The particularly favorable results observed in the T-ALLs and AR2 subgroups suggest the benefit of using DXM in specific patient groups and highlight the importance of risk stratification.</p>","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 11","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11558101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic risk and survival of asymptomatic IgM monoclonal gammopathy: Results from a Spanish Multicenter Registry","authors":"David F. Moreno,&nbsp;Cristina Jiménez,&nbsp;Fernando Escalante,&nbsp;Elham Askari,&nbsp;Marta Castellanos-Alonso,&nbsp;Mario Arnao,&nbsp;Ángela Heredia,&nbsp;Miguel Á. Canales,&nbsp;Magdalena Alcalá,&nbsp;Arancha Bermúdez,&nbsp;Ana Saus Carreres,&nbsp;María Casanova,&nbsp;Luis Palomera,&nbsp;Cristina Motlló,&nbsp;Ricarda García-Sánchez,&nbsp;Pablo Ríos Rull,&nbsp;Ramón García-Sanz,&nbsp;Carlos Fernández de Larrea","doi":"10.1002/hem3.70029","DOIUrl":"10.1002/hem3.70029","url":null,"abstract":"<p>Asymptomatic IgM gammopathy encompasses IgM monoclonal gammopathy of undetermined significance (MGUS) and asymptomatic Waldenström macroglobulinemia (AWM), both having a risk of progression to symptomatic disease. Here, we assessed the risk of progression and the mortality of 956 patients with asymptomatic IgM gammopathy across 25 Spanish centers. After a median follow-up of 5.7 years, 156 patients progressed, most of them to symptomatic WM (SWM). The cumulative incidence of progression was 13% and 20% at 5 and 10 years, respectively. The serum IgM ≥10 g/L, bone marrow (BM) infiltration ≥20%, β2-microglobulin ≥3 mg/L, and albumin &lt;4 g/dL were the most potent predictors of disease progression in a multivariate Cox regression model, allowing the identification of three risk categories. The probability of progression to symptomatic disease at 5 years was 4.5%, 15.7%, and 42.8% for low-, intermediate-, and high-risk groups, respectively. In patients without a BM evaluation, the presence of none or 1 risk factor and 2 or 3 risk factors conferred a progression risk of 6% and 27% at 5 years, respectively. The model was independent of the presence of <i>MYD88</i> L265P, which conferred a negative impact only in AWM patients. The relative survival (RS) ratio at 5 years of asymptomatic patients was similar to the Spanish population, which contrasted with the 0.76 5-year RS of SWM patients. Overall, the Spanish Multicenter Model comprehensively describes the risk of progression of asymptomatic patients and shows that the excess mortality is increased only in the symptomatic stage of the disease.</p>","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 11","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11555297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causes of death among patients diagnosed with chronic lymphocytic leukemia: A population-based study in the Netherlands, 1996–2020","authors":"Lina van der Straten,&nbsp;Mark-David Levin,&nbsp;Manette A. W. Dinnessen,&nbsp;Otto Visser,&nbsp;Eduardus F. M. Posthuma,&nbsp;Jeanette K. Doorduijn,&nbsp;Anton W. Langerak,&nbsp;Arnon P. Kater,&nbsp;Avinash G. Dinmohamed","doi":"10.1002/hem3.70015","DOIUrl":"10.1002/hem3.70015","url":null,"abstract":"<p>Chronic lymphocytic leukemia (CLL) manifests heterogeneously with varying outcomes. This population-based study examined causes of death (CODs), as registered by the physician who established the death, among 20,588 CLL patients diagnosed in the Netherlands between 1996 and 2020. Utilizing cause-specific flexible parametric survival models, we estimated cause-specific hazard ratios (HRs) and cumulative incidences of death due to CLL, solid malignancies, other hematological malignancies, infections, and other causes. Our findings reveal CLL as the predominant COD, contributing to around 40% of relative mortality, with a declining 5-year death probability from 16.8% in 1996–2002 to 7.6% in 2010–2020. Also, deaths attributed to solid malignancies, other hematological malignancies, and other COD diminished over time, as evidenced by respective HRs (95% confidence interval) of 0.68 (0.60%–0.77%), 0.45 (0.38%–0.53%), and 0.77 (0.66%–0.90%). In summary, our comprehensive, population-based analysis underscores a noticeable reduction in CLL-attributed deaths and other competing causes over the studied period. Nonetheless, CLL is registered as the most prevalent cause of mortality among contemporary diagnosed patients with CLL, emphasizing the continued relevance of CLL-centric clinical strategies and research.</p>","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 11","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11555299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic-associated neutropenia is marked by the depletion of intestinal Lachnospiraceae and associated metabolites in pediatric patients","authors":"Josaura Fernandez-Sanchez,&nbsp;Rachel Rodgers,&nbsp;Arushana A. Maknojia,&nbsp;Nusrat Shaikh,&nbsp;Hannah Yan,&nbsp;Marlyd E. Mejia,&nbsp;Hope Hendricks,&nbsp;Robert R. Jenq,&nbsp;Pavan Reddy,&nbsp;Ritu Banerjee,&nbsp;Jeremy M. Schraw,&nbsp;Megan T. Baldridge,&nbsp;Katherine Y. King","doi":"10.1002/hem3.70038","DOIUrl":"10.1002/hem3.70038","url":null,"abstract":"<p>Prolonged antibiotic exposure causes dangerous hematologic side effects, including neutropenia, in up to 34% of patients. Murine studies established a link between the intestinal microbiota and hematopoiesis. To identify factors that predispose to neutropenia in pediatric patients, we evaluated changes in microbiota-derived metabolites and intestinal microbiota composition after prolonged courses of antibiotics. In this multi-center study, patients with infections requiring anticipated antibiotic treatment of two or more weeks were enrolled. Stool samples were obtained at the start and completion of antibiotics or at neutropenia onset (prospective arm). Some patients were enrolled in a retrospective arm in which a stool sample was collected at the time of neutropenia during antibiotic therapy and 2–4 weeks after completion of antibiotics with recovery of blood counts. We identified 10 patients who developed neutropenia on antibiotics and 29 controls matched for age, sex, race, and ethnicity. Clinical data demonstrated no association between neutropenia and the type of infection or antibiotic used; however, patients with neutropenia were admitted to the intensive care unit more often and received longer courses of antibiotics. Reduced intestinal microbiome richness and, specifically, decreased abundance of <i>Lachnospiraceae</i> family members correlated with neutropenia. Untargeted stool metabolomic profiling revealed several metabolites that were depleted exclusively in patients with neutropenia, including members of the urea cycle pathway, pyrimidine metabolism, and fatty acid metabolism that are known to be produced by <i>Lachnospiraceae</i>. Our study shows a relationship between intestinal microbiota disruption and abnormal hematopoiesis and identifies taxa and metabolites likely to contribute to microbiota-sustained hematopoiesis.</p>","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 11","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"30-Minute infusion of isatuximab in patients with newly diagnosed multiple myeloma: Results of a Phase 1b study analysis","authors":"Enrique M. Ocio,&nbsp;Aurore Perrot,&nbsp;Philippe Moreau,&nbsp;Maria-Victoria Mateos,&nbsp;Sara Bringhen,&nbsp;Joaquín Martínez-López,&nbsp;Lionel Karlin,&nbsp;Song-Yau Wang,&nbsp;Corina Oprea,&nbsp;Yi Li,&nbsp;Ercem Kodas,&nbsp;Jesus San-Miguel","doi":"10.1002/hem3.70041","DOIUrl":"10.1002/hem3.70041","url":null,"abstract":"&lt;p&gt;The addition of an anti-CD38 antibody to standard treatment regimens, in combination with an immunomodulatory drug or a proteasome inhibitor and dexamethasone, provides benefit to multiple myeloma (MM) patients in the relapsed/refractory setting (RRMM), as well as at an earlier disease stage in quadruplet combinations for transplant-eligible or non-eligible patients with newly diagnosed MM (NDMM).&lt;span&gt;&lt;sup&gt;1-3&lt;/sup&gt;&lt;/span&gt; Isatuximab (Isa) is approved in various countries in combination with pomalidomide-dexamethasone or carfilzomib-dexamethasone for the treatment of RRMM patients.&lt;span&gt;&lt;sup&gt;4-6&lt;/sup&gt;&lt;/span&gt;&lt;/p&gt;&lt;p&gt;Study findings in transplant-eligible NDMM patients demonstrated significant efficacy of Isa in quadruplet combinations with bortezomib-lenalidomide-dexamethasone (VRd) for induction therapy in the Phase 3 GMMG-HD7 trial (minimal residual disease [MRD] negativity: 50% with Isa-VRd vs 36% with VRd; &lt;i&gt;p&lt;/i&gt; = 0.00017), and with carfilzomib-lenalidomide-dexamethasone (KRd) for induction/consolidation treatment in the Phase 3 IsKia/EMN24 trial (MRD negativity after consolidation: 77% with Isa-KRd vs 67% with KRd; &lt;i&gt;p&lt;/i&gt; = 0.049), without new safety signals.&lt;span&gt;&lt;sup&gt;7, 8&lt;/sup&gt;&lt;/span&gt;&lt;/p&gt;&lt;p&gt;In transplant-ineligible NDMM patients, significant PFS benefit (hazard ratio [HR], 0.60; 98.5% confidence interval [CI], 0.41–0.88; &lt;i&gt;p&lt;/i&gt; &lt; 0.001) and deep, sustained responses were reported with Isa in combination with VRd followed by Isa-Rd versus VRd followed by Rd, in a prespecified interim analysis of the Phase 3 IMROZ trial, with a manageable safety profile.&lt;span&gt;&lt;sup&gt;9&lt;/sup&gt;&lt;/span&gt; In the Phase 3 BENEFIT trial, the addition of weekly bortezomib to Isa-Rd (reduced-dose VRd) induced a significant improvement in MRD negativity at 18 months versus Isa-Rd (53% vs. 26%, &lt;i&gt;p&lt;/i&gt; &lt; 0.0001).&lt;span&gt;&lt;sup&gt;10&lt;/sup&gt;&lt;/span&gt;&lt;/p&gt;&lt;p&gt;The evaluation of Isa with either bortezomib-cyclophosphamide-dexamethasone (VCd) or VRd has shown safety and efficacy of these quadruplet regimens in the Phase 1b trial TCD13983 (NCT02513186) in transplant-ineligible NDMM patients (all cohorts) or patients with no immediate intent for autologous stem cell transplantation included in Isa-VRd/Part-B, as previously reported.&lt;span&gt;&lt;sup&gt;11, 12&lt;/sup&gt;&lt;/span&gt;&lt;/p&gt;&lt;p&gt;To enhance the convenience of long-term treatment with IV Isa for patients and healthcare providers, by improving the current duration of Isa infusion (75 min), we prospectively evaluated the feasibility, safety, and tolerability of a novel, fast, 30-min infusion method for Isa in patients who were on maintenance therapy in the TCD13983 study.&lt;/p&gt;&lt;p&gt;All patients on maintenance treatment, regardless of treatment cohort, were switched to 30-min infusion with Isa at 10 mg/kg (250-mL fixed-volume infusion). Details on study treatments before switching, premedications, and patient characteristics are provided in Supporting Information and Supporting Information S1: Figure S1. To accelerate the infusions to a target durat","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 11","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11538320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to diagnose acid sphingomyelinase deficiency (ASMD) and Niemann–Pick disease type C from bone marrow and peripheral blood smears","authors":"Sandrine Girard,&nbsp;Magali Pettazzoni,&nbsp;Roseline Froissart,&nbsp;Cécile Pagan,&nbsp;Thomas Boyer,&nbsp;Stephanie Dulucq,&nbsp;Valérie Gonçalves Monteiro,&nbsp;Nicolas Lechevalier,&nbsp;Marie Loosveld,&nbsp;Camille Lours,&nbsp;Caroline Mayeur-Rousse,&nbsp;Mélanie Pannetier,&nbsp;Caroline Peillon,&nbsp;Maria-Alessandra Rosenthal,&nbsp;Sonnthida Sep Hieng,&nbsp;Catherine Trichet,&nbsp;Lucile Baseggio,&nbsp;on behalf the French-Speaking Cellular Haematology Group (GFHC)","doi":"10.1002/hem3.70042","DOIUrl":"10.1002/hem3.70042","url":null,"abstract":"&lt;p&gt;Lysosomal storage diseases (LSD) are inborn errors of metabolism disorders characterized by a defect in a lysosomal enzyme, transporter, or cofactor. Niemann–Pick diseases are classified into two distinct disorders: acid sphingomyelinase deficiency (ASMD) historically known as Niemann–Pick disease types A, AB, and B, and Niemann-Pick disease type C (NPC).&lt;span&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/span&gt; ASMD is a rare autosomal recessive LSD, caused by pathogenic variants in the ASM-encoding &lt;i&gt;SMPD1&lt;/i&gt; gene (OMIM#607608).&lt;span&gt;&lt;sup&gt;2&lt;/sup&gt;&lt;/span&gt; It results in the accumulation of sphingomyelin and other lipids, primarily in the liver, spleen, lung, bone marrow, lymph nodes, and central nervous system.&lt;span&gt;&lt;sup&gt;3&lt;/sup&gt;&lt;/span&gt; Depending on their clinical phenotype, three different subtypes have been reported: A (severe infantile neurovisceral form), AB (chronic neurovisceral form), and B (chronic visceral form), with a continuum spectrum.&lt;span&gt;&lt;sup&gt;1-4&lt;/sup&gt;&lt;/span&gt; NPC is an autosomal recessive LSD caused by the defective function of one of two proteins, NPC1 or NPC2. It results from mutations in the corresponding genes (OMIM#257220 and OMIM#607625). These two proteins act in sequence to regulate the egress of endocytosed nonesterified cholesterol from the late endosomal/lysosomal compartment. NPC manifests as a neurovisceral disease with a highly heterogeneous clinical spectrum.&lt;span&gt;&lt;sup&gt;5&lt;/sup&gt;&lt;/span&gt; The prognosis of NPC is correlated with the age of onset of neurological symptoms, with four neurological forms defined: early infantile, late infantile, juvenile, and adolescent-adult. The diagnosis of ASMD and NPC is difficult because these diseases are heterogeneous and may share clinical features with other LSD such as Gaucher disease, especially when splenomegaly is present.&lt;span&gt;&lt;sup&gt;5&lt;/sup&gt;&lt;/span&gt; Some cytological abnormalities have been reported in bone marrow (BM) and peripheral blood (PB) smears from ASMD and NPC patients, which could help to guide the more specific analysis such as enzymatic activity, biomarkers measurement, and genetic testing.&lt;span&gt;&lt;sup&gt;6&lt;/sup&gt;&lt;/span&gt; However, the cytological data available in the literature are rather limited, often described in single case reports, and do not distinguish the different forms of ASMD and NPC. This work aims to report the cytological features of BM and PB in a retrospective study of 30 French cases from 28 families with ASMD types A [&lt;i&gt;n&lt;/i&gt; = 5], AB [&lt;i&gt;n&lt;/i&gt; = 3], B [&lt;i&gt;n&lt;/i&gt; = 16], and NPC [&lt;i&gt;n&lt;/i&gt; = 6], to improve knowledge and define recommendations to assist in diagnosis.&lt;/p&gt;&lt;p&gt;The diagnosis of cases was based on biochemical analysis, specifically either a deficiency in acid sphingomyelinase activity in blood and/or an abnormal plasma biomarkers profile (i.e., lysosphingomyelin, lysosphingomyelin509/&lt;i&gt;N&lt;/i&gt;-palmitoyl-O-phosphocholineserine, and oxysterols), confirmed by specific gene analysis, except for two suspected NPC patients for whom the genetic study was inconclusive and identified a vari","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 11","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11538321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GPRASP protein deficiency triggers lymphoproliferative disease by affecting B-cell differentiation","authors":"Antonio Morales-Hernández,&nbsp;Emilia Kooienga,&nbsp;Heather Sheppard,&nbsp;Gabriela Gheorghe,&nbsp;Claire Caprio,&nbsp;Ashley Chabot,&nbsp;Shannon McKinney-Freeman","doi":"10.1002/hem3.70037","DOIUrl":"10.1002/hem3.70037","url":null,"abstract":"<p><i>Gprasp1</i> and <i>Gprasp2</i> encode proteins that control the stability and cellular trafficking of CXCR4, a master regulator of hematopoiesis whose dynamic regulation is required for appropriate trafficking of B-cells in the germinal center (GC). Here, we report that <i>Gprasp1</i> and <i>Gprasp2</i>-deficient B-cells accumulate in the GC and show transcriptional abnormalities, affecting the mechanisms controlling <i>Aicda</i> expression and exposing them to excessive somatic hypermutation. Consequently, about 30% of mice transplanted with <i>Gprasp</i>-deficient hematopoietic stem and progenitor cells developed a biologically aggressive and fatal B-cell hyperproliferative disease by 20–50 weeks posttransplant. Histological and molecular profiling reveal that <i>Gprasp1-</i> and <i>Gprasp2-</i>deficient neoplasms morphologically resemble human high-grade B-cell lymphomas of germinal center origin with shared morphologic features of both Burkitt Lymphoma (BL) and diffuse large B-cell lymphoma (DLBCL), and molecular features consistent with DLBCL, as well as elevated mutational burden and heterogenous transcriptional and mutational signature. Thus, reduced <i>Gprasp1</i> and <i>Gprasp2</i> gene expression perturbs B-cell maturation and increases the risk of B-cell neoplasms of germinal center origin. As this model recapitulates the essential features of the heterogenous group of human hematopoietic malignancies, it could be a powerful tool to interrogate the mechanisms of lymphomagenesis for these cancers.</p>","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 11","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial retention in mature red blood cells from patients with sickle cell disease is associated with stress erythropoiesis but not with proinflammatory state","authors":"Marc Romana,&nbsp;Sandrine Laurance,&nbsp;Marie-Dominique Hardy-Dessources,&nbsp;Laetitia Claer,&nbsp;Sylvie Ravion,&nbsp;Karim Dorgham,&nbsp;Yohann Garnier,&nbsp;Lea Kuznicki,&nbsp;Vanessa Tarer,&nbsp;Benoit Tressières,&nbsp;Sophie D. Lefevre,&nbsp;Veronique Baccini,&nbsp;Mariano A. Ostuni,&nbsp;Caroline Le Van Kim,&nbsp;Maryse Etienne-Julan","doi":"10.1002/hem3.70030","DOIUrl":"https://doi.org/10.1002/hem3.70030","url":null,"abstract":"&lt;p&gt;Sickle cell disease (SCD) is a hemoglobinopathy characterized by the occurrence of vaso-occlusive events, severe chronic hemolytic anemia, and ultimately chronic complications and end-organ damages.&lt;span&gt;&lt;sup&gt;1-3&lt;/sup&gt;&lt;/span&gt; SCD pathophysiology has been shown to be extremely complex, resulting from microcirculatory dysfunctions associated with altered vaso-regulation and activation of inflammation cascades responsible of sterile inflammatory state, endothelial and neutrophil activation, and release of neutrophil extracellular trap (NET).&lt;span&gt;&lt;sup&gt;1, 4-6&lt;/sup&gt;&lt;/span&gt; More recently, a dysfunctional erythropoiesis has been described in SS patients characterized by high level of reticulocytes, increased apoptosis at the later stage of erythropoiesis, and abnormal retention of mitochondria in red blood cells (RBCs).&lt;span&gt;&lt;sup&gt;7-13&lt;/sup&gt;&lt;/span&gt; It is noteworthy that the functionality of these mitochondria in mature sickle RBCs remains controversial&lt;span&gt;&lt;sup&gt;11, 12&lt;/sup&gt;&lt;/span&gt; and mechanisms responsible for the mitochondrial retention during erythropoiesis have not been identified. Besides these unanswered points, several groups reported &lt;i&gt;in vitro&lt;/i&gt; evidence that plasma mitochondrial DNA released by hemolysis of these abnormal RBCs could trigger type I interferon production&lt;span&gt;&lt;sup&gt;12&lt;/sup&gt;&lt;/span&gt; and NET release in SCD patients.&lt;span&gt;&lt;sup&gt;13&lt;/sup&gt;&lt;/span&gt; Altogether, these studies suggested that mitochondrial DNA from sickle mature RBCs could play a key role in the proinflammatory state associated with the disease.&lt;/p&gt;&lt;p&gt;In the present study, we characterized mature RBCs retaining mitochondria in a large cohort of the two main SCD genotypes, that is, SS and SC adult patients (71 and 40 patients, respectively) compared to 21 AA control individuals. We analyzed associations between mitochondria retention and hemolysis as well as inflammation markers (see patients and methods in Supporting Information and Supporting Information S1: Table 1 for the biological and demographic parameters).&lt;/p&gt;&lt;p&gt;Mitochondria presence in mature RBCs, total, and stress reticulocytes was assessed using flow cytometry (CD71/TO and/or MitoTracker Deep Red (MTKDR) staining) (Figure 1A). SS patients exhibited significant higher percentage of total circulating reticulocytes (5.0% ± 2.2%) compared to AA healthy donors (1.1% ± 0.4%), with a significant intermediate phenotype for SC patients (3.6% ± 1.7%) (Figure 1Bi). SS patients presented significant high levels of stress reticulocytes (2.6% ± 1.2%) compared to very low level observed in AA healthy donors (0.14 ± 0.09) while SC patients exhibited significant intermediate level (1.8% ± 1.0%) (Figure 1Bii). We did not observe significant difference of total and stress reticulocyte percentages between hydroxyurea (HU)-treated and nontreated SS patients (Figure 1Biii,iv). Percentage of mitochondria&lt;sup&gt;+&lt;/sup&gt;-total reticulocytes was significantly higher in SS patients (25.0% ± 13.2%) compared to AA healthy donors (11.9% ± 8.","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 11","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/hem3.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142525355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abstract Book","authors":"","doi":"10.1002/hem3.70012","DOIUrl":"https://doi.org/10.1002/hem3.70012","url":null,"abstract":"&lt;p&gt;Alex F. Herrera&lt;sup&gt;1&lt;/sup&gt;, Michael Leblanc&lt;sup&gt;2&lt;/sup&gt;, Sharon M. Castellino&lt;sup&gt;3&lt;/sup&gt;, Hongli Li&lt;sup&gt;2&lt;/sup&gt;, Sarah Rutherford&lt;sup&gt;4&lt;/sup&gt;, Andrew Evens&lt;sup&gt;5&lt;/sup&gt;, Kelly Davison&lt;sup&gt;6&lt;/sup&gt;, Angela Punnett&lt;sup&gt;7&lt;/sup&gt;, Susan K. Parsons&lt;sup&gt;8&lt;/sup&gt;, Sairah Ahmed&lt;sup&gt;9&lt;/sup&gt;, Carla Casulo&lt;sup&gt;10&lt;/sup&gt;, Nancy L. Bartlett&lt;sup&gt;11&lt;/sup&gt;, Joseph Tuscano&lt;sup&gt;12&lt;/sup&gt;, Matthew Mei&lt;sup&gt;1&lt;/sup&gt;, Brian Hess&lt;sup&gt;13&lt;/sup&gt;, Ryan Jacobs&lt;sup&gt;14&lt;/sup&gt;, Hayder Saeed&lt;sup&gt;15&lt;/sup&gt;, Pallawi Torka&lt;sup&gt;16&lt;/sup&gt;, Boyu Hu&lt;sup&gt;17&lt;/sup&gt;, Craig H. Moskowitz&lt;sup&gt;18&lt;/sup&gt;, Supreet Kaur&lt;sup&gt;19&lt;/sup&gt;, Gaurav Goyal&lt;sup&gt;20&lt;/sup&gt;, Christopher Forlenza&lt;sup&gt;16&lt;/sup&gt;, Andrew Doan&lt;sup&gt;21&lt;/sup&gt;, Adam Lamble&lt;sup&gt;22&lt;/sup&gt;, Pankaj Kumar&lt;sup&gt;23&lt;/sup&gt;, Saeeda Chowdury&lt;sup&gt;24&lt;/sup&gt;, Brett Brinker&lt;sup&gt;25&lt;/sup&gt;, Namita Sharma&lt;sup&gt;26&lt;/sup&gt;, Avina Singh&lt;sup&gt;27&lt;/sup&gt;, Kristie Blum&lt;sup&gt;28&lt;/sup&gt;, Anamarija Perry&lt;sup&gt;29&lt;/sup&gt;, Alexandra Kovach&lt;sup&gt;21&lt;/sup&gt;, David Hodgson&lt;sup&gt;30&lt;/sup&gt;, Louis Constine&lt;sup&gt;10&lt;/sup&gt;, Lale Kostakoglu&lt;sup&gt;31&lt;/sup&gt;, Anca Prica&lt;sup&gt;30&lt;/sup&gt;, Hildy Dillon&lt;sup&gt;32&lt;/sup&gt;, Richard F. Little&lt;sup&gt;33&lt;/sup&gt;, Margaret A. Shipp&lt;sup&gt;34&lt;/sup&gt;, Michael Crump&lt;sup&gt;30&lt;/sup&gt;, Brad S. Kahl&lt;sup&gt;11&lt;/sup&gt;, John Leonard&lt;sup&gt;4&lt;/sup&gt;, Sonali Smith&lt;sup&gt;35&lt;/sup&gt;, Kara M. Kelly&lt;sup&gt;36&lt;/sup&gt;, Jonathan W. Friedberg&lt;sup&gt;10&lt;/sup&gt;&lt;/p&gt;&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;City of Hope, &lt;sup&gt;2&lt;/sup&gt;SWOG Statistics and Data Management Center, &lt;sup&gt;3&lt;/sup&gt;Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, &lt;sup&gt;4&lt;/sup&gt;Weill Cornell Medicine, &lt;sup&gt;5&lt;/sup&gt;Rutgers Cancer Institute of New Jersey, &lt;sup&gt;6&lt;/sup&gt;McGill University Health Center, &lt;sup&gt;7&lt;/sup&gt;SickKids Hospital, &lt;sup&gt;8&lt;/sup&gt;Tufts Medical Center, &lt;sup&gt;9&lt;/sup&gt;MD Anderson Cancer Center, &lt;sup&gt;10&lt;/sup&gt;University of Rochester, &lt;sup&gt;11&lt;/sup&gt;Washington University in St. Louis, &lt;sup&gt;12&lt;/sup&gt;UC Davis, &lt;sup&gt;13&lt;/sup&gt;Medical University of South Carolina, &lt;sup&gt;14&lt;/sup&gt;Levine Cancer Institute, &lt;sup&gt;15&lt;/sup&gt;Moffitt Cancer Center, &lt;sup&gt;16&lt;/sup&gt;Memorial Sloan Kettering Cancer Center, &lt;sup&gt;17&lt;/sup&gt;Huntsman Cancer Institute, University of Utah, &lt;sup&gt;18&lt;/sup&gt;University of Miami, &lt;sup&gt;19&lt;/sup&gt;University of Texas at San Antonio, &lt;sup&gt;20&lt;/sup&gt;University of Alabama at Birmingham, &lt;sup&gt;21&lt;/sup&gt;Children's Hospital of Los Angeles, &lt;sup&gt;22&lt;/sup&gt;Seattle Children's Hospital, &lt;sup&gt;23&lt;/sup&gt;Illinois Cancer Care, &lt;sup&gt;24&lt;/sup&gt;Prisma Health Cancer Institute, &lt;sup&gt;25&lt;/sup&gt;Cancer &amp; Hematology Center, &lt;sup&gt;26&lt;/sup&gt;Geisinger Community Medical Center, &lt;sup&gt;27&lt;/sup&gt;Fairview Ridges Hospital, &lt;sup&gt;28&lt;/sup&gt;Emory University, Winship Cancer Institute, &lt;sup&gt;29&lt;/sup&gt;University of Michigan, &lt;sup&gt;30&lt;/sup&gt;Princess Margaret Cancer Centre, &lt;sup&gt;31&lt;/sup&gt;University of Virginia, &lt;sup&gt;32&lt;/sup&gt;SWOG Cancer Research Network, &lt;sup&gt;33&lt;/sup&gt;National Cancer Institute, &lt;sup&gt;34&lt;/sup&gt;Dana-Farber Cancer Institute, &lt;sup&gt;35&lt;/sup&gt;University of Chicago, &lt;sup&gt;36&lt;/sup&gt;Roswell Park Comprehensive Cancer Center&lt;/p&gt;&lt;p&gt;&lt;b&gt;Figure 1:&lt;/b&gt; Progression-Free Survival in in Modified Intent-to-treat Analysis Set.&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;&lt;b","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 S2","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/hem3.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term outcome of 2-year survivors after allogeneic hematopoietic cell transplantation for acute leukemia","authors":"Marion Larue,&nbsp;Myriam Labopin,&nbsp;Thomas Schroeder,&nbsp;Xiao-jun Huang,&nbsp;Igor W. Blau,&nbsp;Johannes Schetelig,&nbsp;Arnold Ganser,&nbsp;Rose-Marie Hamladji,&nbsp;Wolfgang Bethge,&nbsp;Nicolaus Kröger,&nbsp;Gerard Socié,&nbsp;Urpu Salmenniemi,&nbsp;Henrik Sengeloev,&nbsp;Bhagirathbhai Dholaria,&nbsp;Bipin N. Savani,&nbsp;Arnon Nagler,&nbsp;Fabio Ciceri,&nbsp;Mohamad Mohty","doi":"10.1002/hem3.70026","DOIUrl":"10.1002/hem3.70026","url":null,"abstract":"<p>Information on late complications in patients with acute leukemia who have undergone allogeneic hematopoietic cell transplantation (HCT) is limited. We performed a left-truncated analysis of long-term survival in patients with acute leukemia who were alive and disease-free 2 years after HCT. We included 2701 patients with acute lymphoblastic leukemia (ALL) and 9027 patients with acute myeloid leukemia (AML) who underwent HCT between 2005 and 2012. The 10-year overall survival (OS) rate was 81.3% for ALL and 76.2% for AML, with the main causes of late mortality being relapse (ALL-33.9%, AML-44.9%) and chronic graft-versus-host disease (ALL-29%, AML-18%). At 10 years, HCT-related mortality was 16.8% and 20.4%, respectively. Older age and unrelated donor transplantation were associated with a worse prognosis for both types of leukemia. In addition, transplantation in the second or third complete remission and peripheral blood HSC for ALL are associated with worse outcomes. Similarly, adverse cytogenetics, female donor to male patient combination, and reduced intensity conditioning in AML contribute to poor prognosis. We conclude that 2-year survival in remission after HCT for acute leukemia is encouraging, with OS of nearly 80% at 10 years. However, the long-term mortality risk of HCT survivors remains significantly higher than that of the age-matched general population. These findings underscore the importance of tailoring transplantation strategies to improve long-term outcomes in patients with acute leukemia undergoing HCT.</p>","PeriodicalId":12982,"journal":{"name":"HemaSphere","volume":"8 10","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11494155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}