Hanieh Gholamalizadeh, Vafa Baradaran Rahimi, Vahid Reza Askari
{"title":"Orphan G Protein-Coupled Receptors: A Novel Research Frontier in Autism and Associated Disorders","authors":"Hanieh Gholamalizadeh, Vafa Baradaran Rahimi, Vahid Reza Askari","doi":"10.1002/ardp.70018","DOIUrl":"https://doi.org/10.1002/ardp.70018","url":null,"abstract":"<div>\u0000 \u0000 <p>G-protein coupled receptors (GPCRs) are a diverse group of membrane proteins crucial for transmitting extracellular signals into cells, regulating numerous neurological processes. Among GPCRs, orphan receptors, whose natural ligands and functions remain unidentified, have garnered increasing research attention. Olfactory receptors (ORs), the most diverse GPCR family, are widely expressed in the central nervous system (CNS) and are implicated in autism spectrum disorder (ASD) and related behavioral and psychiatric conditions. This review highlights the altered expression of specific orphan and olfactory GPCRs—such as RORα, RORβ, GPR37, GPR62, OR1C1, and OR52M1—and their roles in ASD development. It explores their mechanisms of action, neural network interactions, and contributions to ASD etiology, as well as associated disorders like ADHD, schizophrenia, epilepsy, anxiety, depression, and sleep disorders. The review also addresses challenges in orphan GPCR research, such as identifying ligands and understanding their physiological roles, while emphasizing their potential as therapeutic targets. By summarizing preclinical and clinical findings, this review underscores the emerging significance of orphan receptors in ASD, offering a foundation for future research to advance understanding and develop innovative treatments for autism and related conditions.</p></div>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alessia Costa, Murat Bozdag, Gioele Renzi, Barbara Rani, Maria Beatrice Passani, Andrea Angeli, Gustavo Provensi, Fabrizio Carta, Claudiu T. Supuran
{"title":"Hitting Two Birds With One Stone: Dual Modulation of Brain Carbonic Anhydrases and Histone Deacetylases Boosts Memory Consolidation","authors":"Alessia Costa, Murat Bozdag, Gioele Renzi, Barbara Rani, Maria Beatrice Passani, Andrea Angeli, Gustavo Provensi, Fabrizio Carta, Claudiu T. Supuran","doi":"10.1002/ardp.70020","DOIUrl":"https://doi.org/10.1002/ardp.70020","url":null,"abstract":"<p>Cognitive impairments, characterized by deficits in one or more cognitive domains, are common in several pathological conditions but remain inadequately addressed by available pharmacological treatments. Given the complexity and multifaceted mechanisms underpinning these deficits, multi-targeted directed ligands are emerging as promising strategies for developing more effective therapies. In this study, we reported the design, synthesis as well as In Vitro and Ex Vivo assessment of prototypic molecular scaffolds that activate the carbonic anhydrase (CA; EC 4.2.1.1) and inhibit the histone deacetylase (HDAC; EC 3.5.1.98) metalloenzymes. By using the novel object recognition paradigm, we found that these compounds significantly enhanced memory consolidation at doses 10 times lower than single-target reference compounds. Taken together, these results suggest that the dual modulation of CA and HDAC activities by means of a single hybrid molecular entity represents an innovative approach for the management of cognitive symptoms associated with neurodegenerative, neurodevelopment, and psychiatric disorders.</p>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ardp.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Therapeutic Potential of Berberine in Lung Cancer","authors":"Zohreh Jafari, Shokouh Honarmand, Zeinab Abbasirad, Sahar Sadeghi, Ashkan Bigham","doi":"10.1002/ardp.70013","DOIUrl":"https://doi.org/10.1002/ardp.70013","url":null,"abstract":"<div>\u0000 \u0000 <p>Lung cancer has the second-highest incidence rate after breast cancer and remains the leading cause of cancer-related mortality. The 1-year survival rate for lung cancer patients is below 50%, highlighting the urgent need for novel therapeutic strategies and drug development. Phytochemicals and their derivatives have been widely explored for their anticancer properties, serving as chemotherapeutic agents against various types of cancer. One of these herbal compounds, berberine (BBR), a quaternary isoquinoline alkaloid, has shown significant promise in preclinical studies and is currently undergoing clinical trials for cancer treatment. BBR exhibits diverse biological activities, contributing to its anticancer potential, including antioxidant, antidiarrheal, antidiabetic, antimicrobial, and so on. However, despite its multifunctional therapeutic potential, BBR faces several limitations, hindering its clinical application, like poor bioavailability, low tissue uptake, a short plasma half-life, and rapid metabolic elimination. To address these challenges, various targeted drug delivery approaches have been developed to improve its efficacy. This review aims to provide a comprehensive overview of drug delivery strategies designed to encapsulate BBR for enhanced lung cancer therapy, highlighting the most recent advancements in the field. Moreover, the molecular structure of BBR and the biological pathways it targets to inhibit lung cancer progression are discussed in detail. Finally, BBR-encapsulated nanocarriers specifically developed for lung cancer therapy are evaluated in terms of their benefits, limitations, and overall therapeutic potential.</p>\u0000 </div>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aripiprazole as an Inhibitor of the EGFR/PI3K/AKT Signaling Pathway and Sensitizer of MPA Suppressed Progestin-Resistant Endometrial Cancer Cells","authors":"Wen Gan, Xinyu Liu, Yikang Zhou, Wei Gu, Xiaohu Liu, Lingyi Song, Jian Li, Yudong Wang, Fei Mao","doi":"10.1002/ardp.70017","DOIUrl":"https://doi.org/10.1002/ardp.70017","url":null,"abstract":"<div>\u0000 \u0000 <p>Drug repurposing, which involves applying existing pharmaceuticals to new therapeutic areas, offers several advantages, including increased efficiency, reduced costs, and lower risks. The research aimed to investigate the molecular mechanisms underlying the new application of the antipsychotic drug aripiprazole (APZ) in the treatment of endometrial cancer (EC), as well as its synergistic efficacy when used in combination with progestin. The cell viability assay and proliferation assay demonstrated that APZ exerted a significant inhibitory effect on the proliferation of ISK and KLE cells. Moreover, APZ was found to induce cell apoptosis prominently by flow cytometry. Network pharmacology analysis indicated that the anti-EC effects of APZ were mediated via the epidermal growth factor receptor (EGFR)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway, which was subsequently confirmed by Western blot analysis. Furthermore, APZ significantly enhanced the proliferation inhibitory effect of medroxyprogesterone acetate (MPA) against progestin-resistant KLE cells, displaying remarkable synergistic activity. These findings position APZ as a promising therapeutic candidate for EC, with potential utility both as a monotherapy and in combination with MPA, offering new avenues for EC treatment strategies.</p></div>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synthesis, Characterization and Evaluation of Antioxidant and Antimicrobial Activities of Novel Isatin Derivatives","authors":"Komal Rathi, Divya Kumari, Priyanka Yadav, Varun Rawat, Pracheta Janmeda, Ved Prakash Verma","doi":"10.1002/ardp.70014","DOIUrl":"https://doi.org/10.1002/ardp.70014","url":null,"abstract":"<div>\u0000 \u0000 <p>The pursuit of multifunctional therapeutic agents remains crucial in combating oxidative stress and microbial resistance. In this study, we synthesized and characterized a diverse series of novel isatin-based derivatives (<b>12a–z</b>), exploring their antioxidant and antimicrobial potential. Notably, compound <b>12c</b> demonstrated remarkable antioxidant efficacy among all isatin-based derivatives, with an IC₅₀ value of 9.79 ± 0.168 µg/mL in the DPPH radical scavenging assay, positioning it as a potent radical scavenger comparable to the standard (ascorbic acid). Furthermore, antimicrobial screening identified compounds <b>12a</b> and <b>12b</b> as standout candidates, showcasing strong antibacterial and antifungal properties through disc-diffusion and broth dilution methods. These promising bioactivities underscore the potential of <b>12a</b>, <b>12b</b> and <b>12c</b> as therapeutic agents, meriting further investigation for their application in oxidative stress-related conditions and infectious disease management. This study lays the groundwork for future development of isatin derivatives as pharmacologically relevant molecules in both antioxidant and antimicrobial domains.</p></div>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmet Fatih Sahin, Simone Carradori, Alessia Ricci, Muhammad Rashad, Atilla Akdemir
{"title":"The Quest for Ligands Against Kinesin Motor Protein Eg5","authors":"Ahmet Fatih Sahin, Simone Carradori, Alessia Ricci, Muhammad Rashad, Atilla Akdemir","doi":"10.1002/ardp.70010","DOIUrl":"https://doi.org/10.1002/ardp.70010","url":null,"abstract":"<div>\u0000 \u0000 <p>The Kinesin Eg5 motor protein is essential for the formation of the mitotic spindles and chromosome segregation, which is necessary for cell division. The inhibition of Eg5 has emerged as a potential therapeutic strategy for inhibiting the uncontrolled proliferation of cancer cells. This study aims to identify inhibitors targeting the allosteric site I of Eg5. To this end, a hierarchical virtual screening was applied to screen the ZINC20-Anodyne compound library comprising approximately 11 million druglike compounds. The procedure includes a shape similarity screening, a pharmacophore screening, docking studies with subsequent re-scorings, molecular dynamics simulations, and Molecular Mechanics-Generalized Born Surface Area (MM-GBSA) binding free energy calculations and resulted in the selection of four compounds for Eg5 inhibition assays. One compound displays an IC<sub>50</sub> value of 29 µM, while the remaining three compounds show weaker inhibition of Kinesin Eg5. As such, structurally novel Kinesin Eg5 inhibitors have been obtained for further hit-to-lead optimization programs.</p>\u0000 </div>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mine Ensoy, Damla Nur Parıltı, Ayşe Hale Alkan, Kübra Nur Kaplan İlhan, Pelin Mutlu, Bala Gür Dedeoğlu, Demet Cansaran-Duman
{"title":"Evernic Acid: A Low-Toxic and Selective Alternative to Chemotherapeutic Agents in the Treatment of Ovarian Cancer","authors":"Mine Ensoy, Damla Nur Parıltı, Ayşe Hale Alkan, Kübra Nur Kaplan İlhan, Pelin Mutlu, Bala Gür Dedeoğlu, Demet Cansaran-Duman","doi":"10.1002/ardp.70015","DOIUrl":"https://doi.org/10.1002/ardp.70015","url":null,"abstract":"<p>Evernic acid (EA) has emerged as a potential therapeutic agent with its low toxicity and anticancer properties. In this study, the anticancer effect of EA on ovarian cancer cell lines and normal ovarian surface epithelial cells (OSE) was evaluated. The antiproliferative effect of EA was evaluated by xCELLigence Real-Time Cell analysis, colony formation assay, and acridine orange and DAPI staining methods. Genotoxicity analysis was performed by comet assay. The effect of EA on cell migration was analyzed by wound healing assay. The potential of EA to induce apoptosis was also determined by evaluating the changes in gene and protein expression levels by qRT-PCR and Western blot analysis, respectively. EA was found to be a promising potential therapeutic agent for ovarian cancer without showing significant cytotoxic effect on normal cells. Furthermore, EA decreased the ability of ovarian cancer cells for migration, increased the rate of apoptosis by inhibiting BIRC5 and activating CASP3, triggered cell cycle arrest in the G2/M phase, and caused a decrease in mitochondrial membrane potential and genotoxic effects. The results have shown that EA could be an effective candidate molecule for ovarian cancer treatment.</p>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ardp.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Design and Synthesis of O-Dialkylaminoalkyl Substituted Urolithin Derivatives: DNA Topoisomerase IIα Inhibition With Promising Antiproliferative Activity","authors":"Xintong Li, Xiaochun Zhang, Chan Yin, Chunhua Lu, Yuemao Shen","doi":"10.1002/ardp.70009","DOIUrl":"https://doi.org/10.1002/ardp.70009","url":null,"abstract":"<div>\u0000 \u0000 <p>Based on the pharmacophore structural characteristics of topoisomerase II (TopoII) inhibitors: (i) planar polyaromatic skeleton; (ii) the cation core; (iii) a groove-binding side chain moiety, 16 urolithin derivatives with <i>O</i>-dialkylaminoalkyl substitutions were designed and synthesized. Most of the synthesized compounds showed improved TopoIIα inhibitory and antiproliferative activities. <b>20</b> with the best TopoIIα inhibitory activity also exhibited excellent antiproliferative activity with IC<sub>50</sub> values of 0.91 ± 0.01, 1.93 ± 0.04, and 2.84 ± 0.34 μM against the MDA-MB-231, HeLa, and A549 cell lines, being about 12.55, 3.95, and 2.17 times more active than VP-16 (IC<sub>50</sub> 11.42 ± 0.82, 7.63 ± 0.46, and 6.15 ± 0.43 μM, respectively). Meanwhile, <b>20</b> exhibited weak toxicity to normal cells. In addition, <b>20</b> exerted anti-migration and anti-invasion activity against MDA-MB-231 cells. Our results supported that <b>20</b> might act as TopoIIα inhibitor with the potential to become a new type of antitumor drug lead.</p></div>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Erratum: Targeting the Isoprenoid Pathway in Choleste Biosynthesis: An Approach to Identify Isoprenoid Biosynthesis Inhibitors","authors":"","doi":"10.1002/ardp.70016","DOIUrl":"https://doi.org/10.1002/ardp.70016","url":null,"abstract":"<p>by Maximilian Liebl, Florian Olander, Christoph Müller</p><p>In the title of the article published as <i>Arch. Pharm</i>. 2025;358:e2400807, https://doi.org/10.1002/ardp.202400807, the word cholesterol was misrepresented as cholestero. The correct title is:</p><p>Targeting the isoprenoid pathway in cholesterol biosynthesis: An approach to identify isoprenoid biosynthesis inhibitors</p>","PeriodicalId":128,"journal":{"name":"Archiv der Pharmazie","volume":"358 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ardp.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}