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Surface topography of Acanthamoeba culbertsoni extracted from cerebrospinal fluid. 从脑脊液中提取的卡伯氏棘阿米巴表面形貌。
Health laboratory science Pub Date : 1978-10-01
T G Sarphie, D J Allen
{"title":"Surface topography of Acanthamoeba culbertsoni extracted from cerebrospinal fluid.","authors":"T G Sarphie,&nbsp;D J Allen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An axenic culture of Acanthamoeba culbertsoni, strain A-1, was obtained from the American Type Culture collection and injected, via the cisterna magna, into the subarachnoid space of dogs. Attention was focused on the pleomorphic surface specialization of the pathogens suspended in cerebrospinal fluid and compared with those from trypticase soy broth suspensions. Light and scanning electron microscopic comparisons of both inoculated and stock (control) specimens demonstrated similar surface features such as microvillous-like to bleb-like microappendages projecting from variably smooth to undulating membranous exteriors. This study introduces a potentially useful morphological technique that could possibly be of assistance in the more rapid diagnosis of these infections facilitated by SEM screening of the cerebrospinal fluid.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 4","pages":"235-41"},"PeriodicalIF":0.0,"publicationDate":"1978-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11945905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Documentation: the first step in laboratory management. 文件编制:实验室管理的第一步。
Health laboratory science Pub Date : 1978-10-01
L A Stanifer
{"title":"Documentation: the first step in laboratory management.","authors":"L A Stanifer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This article will try to point out an often over-looked tool of management that most laboratory technologists are familiar with. The laboratory technologists that suddenly find themselves as chief technologists or managers would do well to remember their bench training. Specifically, document everything you do. The article lists suggestions and examples pertaining to documentation, that chief technologists could well benefit from. Four specific examples of documentation forms are as follows, (1) Pre-employment evaluation interview, (2) Laboratory orientation check list, (3) Documentation of employee counseling, and (4) Report of corrective interview.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 4","pages":"227-34"},"PeriodicalIF":0.0,"publicationDate":"1978-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11945904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preservation and storage of pathogenic Neisseria. 病原奈瑟菌的保存与贮藏。
Health laboratory science Pub Date : 1978-10-01
R M Cody
{"title":"Preservation and storage of pathogenic Neisseria.","authors":"R M Cody","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pathogenic species of Neisseria, especially N. gonorrhoeae are notoriously difficult to maintain in a viable state for long storage periods. Frequent transfer of cultures on artificial media and storage at 4 to 8 C quickly leads to loss of viability and virulence and to changes in antigenicity. Freeze-drying has been reported as a convenient method for long-term storage of these organisms; however, many laboratories are not properly equipped to use this method. A simple, inexpensive, and efficient procedure is described for storage of N. gonorrhoeae or N. meningitidis for 12 and 18 months, respectively. Results of this work may provide the basis for an efficient method of transporting these organisms over long distances via mail.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 4","pages":"206-9"},"PeriodicalIF":0.0,"publicationDate":"1978-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11326025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wescodyne: lack of activity against poliovirus in the presence of organic matter. Wescodyne:在有机物存在的情况下对脊髓灰质炎病毒缺乏活性。
Health laboratory science Pub Date : 1978-07-01
A M Wallbank, M Drulak, L Poffenroth, C Barnes, C Kay, I Lebtag
{"title":"Wescodyne: lack of activity against poliovirus in the presence of organic matter.","authors":"A M Wallbank,&nbsp;M Drulak,&nbsp;L Poffenroth,&nbsp;C Barnes,&nbsp;C Kay,&nbsp;I Lebtag","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The National Institute of Health Guidelines for Recombinant DNA Research recommends 2% aqueous Wescodyne, an iodophore that is used in many hospitals and laboratories as a disinfectant, as a decontaminant for biological safety cabinets and 5% for a spill outside a cabinet. A contact time of 10 to 15 minutes was given for the 2% solution and 20 minutes was considered adequate for the 5% concentration. The results indicate: 1. Aqueous Wescodyne (5%) is ineffective when used for 80 minutes against poliovirus in a test mixture containing 8.5% bovine serum albumin (a mixture equivalent in protein concentration to the higher range in serum). 2. Wescodyne (10%) employed under the same conditions for 40 minutes is also ineffective. 3. Wescodyne (10% v/v) in 50% ethanol (w/w) was effective and this mixture, originally recommended for hand washing, should be considered for use in biohazard situations, particularly for decontamination of work surfaces and biological safety cabinets. These results are of significance for if a virucide cannot inactivate poliovirus one would be concerned about using the virucide against hepatitis B or SV40 viruses.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 3","pages":"133-7"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11427744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolism of glycosides by Pseudomonas maltophilia. 嗜麦芽假单胞菌对糖苷的代谢。
Health laboratory science Pub Date : 1978-07-01
S K Frank, V L Von Riesen
{"title":"Metabolism of glycosides by Pseudomonas maltophilia.","authors":"S K Frank,&nbsp;V L Von Riesen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A study of the abilities of 23 strains of Pseudomonas maltophilia to hydrolyze synthetically-prepared and naturally-occurring glycosides is presented. Direct detection of liberated aglycones was used to determine hydrolysis of the five most-commonly-used glycosides (amygdalin, arbutin, esculin, ONPG (o-nitrophenyl-beta-d-galactoside), and salicin). The capabilities of the strain for acid production from 17 glycoside substrates were also determined using a medium designed to minimize the production of acid-neutralizing end-products from peptones.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 3","pages":"168-73"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11465990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid identification of San Filippo disease (MPS III). 快速鉴定圣菲利波病(MPS III)。
Health laboratory science Pub Date : 1978-07-01
S Kelly, L Schedlbauer, E Leikhim
{"title":"Rapid identification of San Filippo disease (MPS III).","authors":"S Kelly,&nbsp;L Schedlbauer,&nbsp;E Leikhim","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Humbel's thin-layer chromatography of urine was a rapid, reliable means of identifying type of mucopolysaccharide storage disease in 30 patients. Over half had Hurler-Scheie or Hunter disease (MPS I-V or II); eight had San Filippo disease, of which four expressed the Type B form.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 3","pages":"123-7"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11370322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lead-erythrocyte protoporphyrin correlation in blood of exposed workers. 接触工人血铅与红细胞原卟啉的相关性。
Health laboratory science Pub Date : 1978-07-01
F Peter, R G Reynolds
{"title":"Lead-erythrocyte protoporphyrin correlation in blood of exposed workers.","authors":"F Peter,&nbsp;R G Reynolds","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A systematic survey of blood samples from 174 industrial workers occupationally exposed to lead fumes and lead dust, revealed some poor correlations between the lead (Pb) level and the free erythrocyte protoporphyrin (FEP) level. In addition to those workers who had an expected correlation (normal Pb-normal FEP or elevated Pb-elevated FEP) there were two groups with atypical correlations. In one group a normal Pb level was associated with an elevated FEP level and in the other group an elevated Pb level was associated with a normal FEP level. This shows that neither the Pb nor the FEP test can substitute for the other. Since the individual FEP response to Pb absorption is unpredictable, both tests should be carried out routinely. This would make it possible to determine simultaneously the degree of absorption (Pb level) and the degree of intoxication (FEP level) and would improve significantly the assessment of undue lead absorption of occupationally exposed workers. If, however, only one of these tests is used for screening the other test should always be carried out as a confirmatory test when the screening detects an elevated Pb or FEP level.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 3","pages":"144-9"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11901245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An indirect fluorescent antibody technique for Haemophilus ducreyi. 杜氏嗜血杆菌的间接荧光抗体技术。
Health laboratory science Pub Date : 1978-07-01
G A Denys, T A Chapel, C D Jeffries
{"title":"An indirect fluorescent antibody technique for Haemophilus ducreyi.","authors":"G A Denys,&nbsp;T A Chapel,&nbsp;C D Jeffries","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An indirect fluorescent antibody technique which allows the detection of antibody against or the recognition of Haemophilus ducreyi has been developed. Heat inactivated antiserum was reacted uith prepared smears of Haimophilus species and selected organisms commonly associated with penile ulcerations. In serum absorbed with Haemophilus sp no fluorescence was observed with 156 heterologous organisms, but all six strains of bacteriologically confirmed H. ducreyi gave significant reactions with the absorbed anti-WD-68 serum.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 3","pages":"128-32"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11568514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endemic Serratia marcescens in the Veterans Administration Hospital in Pittsburgh, Pa., 1971--1976. 宾夕法尼亚州匹兹堡退伍军人管理局医院的地方性粘质沙雷氏菌。, 1971 - 1976。
Health laboratory science Pub Date : 1978-07-01
A Brown, L Davis, R B Yee, B Postic
{"title":"Endemic Serratia marcescens in the Veterans Administration Hospital in Pittsburgh, Pa., 1971--1976.","authors":"A Brown,&nbsp;L Davis,&nbsp;R B Yee,&nbsp;B Postic","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The incidence of infections due to Serratia marcescens increased steadily at the Veterans Administration Hospital in Pittsburgh, Pennsylvania from 1970 to mid 1975 followed by a decline extending into 1977. One hundred eighty-four Serratia marcescens isolates were collected from 123 patients over a 4-month period in 1975 and were characterized by stereotype and antibiotic sensitivity. Three-fourths of the isolates were clinically significant. Among 24 stereotypes O-:H2 predominated in the urinary tract and O-H12 in respiratory secretions. Clusters of serotypes were on occasion identified in the GU Surgery Ward and the Intensive Care Unit. Uninary isolates showed remarkable resistance to antibiotics; only two thirds were susceptible to amikacin, the most effective agent in vitro. The patients from whom Serratia was isolated were older than the general hospital population (60 vs. 53.6 years of age), were hospitalized longer than the age-corrected controls (62 vs. 34.5 days) and two-thirds of thirds of them acquired the infection in the hospital. Uninary catheterization and prior antibiotic therapy were significant risk factors for urinary infections, and prior surgery contributed to recovery of Serratia from the respiratory tract. Serotype and antibiotype differences between isolates from the urinary and those from the respiratory tract suggest that strains evolved a site specificity.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 3","pages":"159-67"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11568515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proposed microbiologic guidelines for respiratory therapy equipment and materials. 呼吸治疗设备和材料微生物学指南建议。
Health laboratory science Pub Date : 1978-07-01
{"title":"Proposed microbiologic guidelines for respiratory therapy equipment and materials.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 3","pages":"177-9"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11568516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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