49最新文献

{"title":"The antimicrobial peptide gramicidin S alters proliferation and inhibits adhesion of L929 cell line fibroblasts","authors":"N. M. Alabedalkarim, V. P. Berest, N. M. Moiseieva, G. Bozhok, T. P. Bondarenko","doi":"10.26565/2075-3810-2023-49-04","DOIUrl":"https://doi.org/10.26565/2075-3810-2023-49-04","url":null,"abstract":"Background: Natural antimicrobial peptides are used in the fight against pathogens resistant to existing synthetic antibiotics. The non-specific mechanism of cytostatic action of antimicrobial peptides, in particular gramicidin S, against bacteria is also effective for damaging the cells of neoplasms. The existence of such a property in a registered antibiotic will indicate its antineoplastic potential and can be used to expand the spectrum of its therapeutic application. Aim of work is to clarify the possible antitumor effect of the antimicrobial peptide gramicidin S. Materials and Methods: Using the methods of confocal laser microscopy and light microscopy, the morphological and functional features of connective tissue cells under the influence of gramicidin S in the concentration range 0.5–50 μg/ml were studied using L929 fibroblasts cell culture. The cell area, nucleus area, and nucleus-to-cytoplasm ratio were determined. To study the migratory and proliferative activity of cells in vitro, the “scratch assay” was used, the confluency of the monolayer of cells was evaluated, morphometric studies were performed, and the relative area of the scratch was measured after 24, 48, and 72 hours. Results: The lytic effect of gramicidin S in a concentration of 50 μg/ml on L929 cells was established, in concentrations of 0.5 μg/ml and 5.0 μg/ml, the antibiotic increases the synthetic activity of cells and stimulates the proliferation of fibroblasts in a monolayer. Cell anisomorphism is more pronounced in the presence of 5.0 μg/ml gramicidin S added to the culturing medium during monolayer formation, while a one-third of the cells in the sample form a population that is morphologically different from other cells in the culture. The addition of gramicidin S at non-lytic concentrations of 0.5 and 5.0 μg/ml to unattached fibroblasts reliably inhibits monolayer formation. Under the influence of 5.0 μg/ml gramicidin S, the rate of monolayer formation is low, even despite the significant content of cells with a high nuclear-cytoplasmic ratio. The kinetics of filling the cell monolayer defect using the “scratch assay” shows that GS in concentrations of 0.5 and 5.0 μg/ml can control the migratory and proliferative properties of L929 cells. Conclusions: The effect of gramicidin S on the morphometric parameters of cells depends on the concentration of the peptide and the cell status in the culture. GS corrupts the adhesive properties of L929 fibroblasts in monolayer cell culture and the rate of cell monolayer formation. Cells at the stage of attachment and monolayer formation were most sensitive to non-lytic concentrations of GS. Inhibition of the adhesive properties of connective tissue cells by gramicidin S is a new non-canonical effect of a known antimicrobial drug, which may indicate the possibility of using gramicidin S as an anti-neoplasm agent.","PeriodicalId":127734,"journal":{"name":"49","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139350901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of salt stress and cooling regimes on the pigment composition of Dunaliella salina cells","authors":"N. Chernobai, N. Kadnikova, K. Vozovyk, L. Rozanov, I. F. Kovalenko","doi":"10.26565/2075-3810-2023-49-03","DOIUrl":"https://doi.org/10.26565/2075-3810-2023-49-03","url":null,"abstract":"Background: Microalgae are able to produce a significant amount of biologically significant substances. In connection with the growing popularity of microalgae, it is important to develop effective methods for storing cultures and creating strain banks. This will not only meet the needs of science and biotechnology for viable and sustainable crops, but will also solve the problem of biodiversity conservation. Objectives: study the effect of salt stress and cooling regimes on the pigment composition of microalgae Dunaliella salina cells in order to increase their safety after freezing-thawing. Materials and methods: The objects of the study were the unicellular green microalgae D. salina. Cultivation was carried out according to the standard method on nutrient media with different amounts of NaCl and trace elements. Adaptation to low temperatures was carried out by exposure of samples in the dark at temperature 4°C for 24 hours. Freezing was performed by placing 1 ml of the cell suspension in a 1.8 ml polypropylene cryogenic vial (Nunc, Sigma-Aldrich), cooled at a rate of 1 deg/min using a Mr. Frosty with following regimes: to -10°С, -40°С, -40°С followed by immersion to liquid nitrogen or direct immersion to liquid nitrogen (-196°С). Thawing was carried out in a water bath (37°С) with continuous shaking for 1–2 min. Microscopic examinations were carried out on an LSM-510 Meta laser scanning microscope (Carl Zeiss, Germany) upon excitation by a diode laser with a wavelength of 405 nm and 573 nm using a Nile Red stain. Results: It has been established that the formation of intracellular lipid globules and the synthesis of carotenoids in D. salina cells contribute to an increase in the concentration and number of motile cells after freezing-thawing. It has been shown that during rapid cooling, adaptive mechanisms do not have time to turn on in cells, and complete destruction of carotene-containing lipid globules occurs. Conclusions: Cryopreservation of D. salina cells should be carried out at a rate of 1 deg/min to -40°C, followed by immersion in liquid nitrogen and a mandatory stage of precultivation at 4°C for 24 hours. This approach allows the cells to adapt to a decrease in temperature, which contributes to the best result after freezing-thawing.","PeriodicalId":127734,"journal":{"name":"49","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139352107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular docking of human serum albumin with penicillin G determinants","authors":"N. V. Khmil, V. G. Kolesnikov","doi":"10.26565/2075-3810-2023-49-01","DOIUrl":"https://doi.org/10.26565/2075-3810-2023-49-01","url":null,"abstract":"Background: Human serum albumin (HSA) is the main pharmacokinetic effector of many medications, including penicillin G and its metabolites. An urgent problem of practical medicine is immediate hypersensitivity reactions caused by penicillin toxicity (about 8 % compared to other medications), accompanied by skin pathology, anaphylaxis, and fatality. Objectives: The aim of this study is to describe the structures of penicillin G determinants-HSA complexes and to identify favorable binding sites and the amino acid residues involved in the interaction. Material and Methods: The crystal structure of HSA (ID: 1AO6 from Protein Data Bank) (www.rcsb.org) was chosen as a docking target. Molecular docking methods (AutoDock Tools 1.5.7, AutoDock Vina 1.1.2) were used to gain insight into the interaction of HSA with the major (benzyl penicilloyl G, penicillanic acid) and minor (penicillamine, penicilloic acid, penilloic acid) determinants of penicillin G. Visualization of docking results was implemented in PyMol 2.5. The Protein Plus server (https://proteins.plus) was used to evaluate potential binding pockets. The PLIP tool (https://plip-tool.biotec.tu-dresden.de) was used to identify non-covalent interactions between HSA and its ligands. Results: The molecular docking data indicate that the major determinants of penicillin G are involved in the formation of hydrogen bonds with such HSA residues as Trp214, Arg218, His242, and Asn295; for the minor determinants — Asp108, His146, Tyr148, Ser193, Arg197, Gln204. Both types of determinants are located in the hydrophobic cavity of subdomains IIA and IB. Hydrophobic interactions are present mainly between penicillin G determinants and amino acid residues of subdomain IIIA, such as Ala350, Asp451, Tyr452, and Gln459. Conclusion: The study of penicillin G determinants-HSA complexes is important in the pathogenesis of antibiotic allergy. Identification of specific binding sites can be useful for the development and synthesis of new immunogenic antigens (complexes of major and minor determinants of penicillin G with HSA) that can stimulate the immune system and produce specific antibodies to prevent allergic reactions.","PeriodicalId":127734,"journal":{"name":"49","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass spectrometry study of ascorbyl palmitate as an agent for nanosomes formation","authors":"V. Pashynska, M. Kosevich, P. Kuzema, A. Gomory, L. Drahos","doi":"10.26565/2075-3810-2023-49-02","DOIUrl":"https://doi.org/10.26565/2075-3810-2023-49-02","url":null,"abstract":"Background. Study of properties and intermolecular interactions of biologically active compounds which can be used for the purposes of transmembrane drugs delivery is a topical task of modern molecular biophysics. Ascorbyl Palmitate (AP) as a fat-soluble form of vitamin C has recently attracted attention as a promising agent for formation of nanosomes for the “fat insoluble” drug molecules transfer through membranes. However, AP is not sufficiently characterized by up-to-date soft ionization mass spectrometric techniques. Objectives. The aim of the present work is to characterize AP and its intermolecular interactions by a number of mass spectrometric techniques: Electrospray Ionization (ESI), Laser Desorption/Ionization (LDI) and Matrix-Assisted Laser Desorption/Ionization (MALDI). The comparison of these techniques applicability to the study of AP intermolecular interactions as a drug delivery assisting agent is scheduled. Methods. ESI mass spectra are obtained with triple quadrupole Micromass Quattro mass spectrometer. LDI and MALDI experiments are performed by Autoflex II mass spectrometer. Results. In the ESI experiments in the positive ion mode abundant peaks of protonated and cationized AP molecules as well as the peaks of AP clusters nAP•H+ and nAP•Na+ (n=2÷4) are revealed in the mass spectra. This result testifies to the formation of stable noncovalent complexes of the AP molecules in the polar media and confirms the AP ability of formation nanosomes for drug delivery. Analysis of LDI and MALDI mass spectra of AP in positive and negative ion modes shows that in the presence of molecular ions of AP, the peaks of AP dimers or larger AP clusters are not recorded. The ESI probing of the model system containing AP and dipalmitoylphosphatidylcholine (DPPC) reveals stable AP•DPPC•H+ complex which models the AP intermolecular interactions with the phospholipid components of biomembranes and/or liposomes under AP functioning as a drug delivery assisting agent. Conclusions. The current study demonstrates the applicability of all tested mass spectrometric techniques for AP identification in solutions and solid phase, while for the purpose of examining of the AP noncovalent complexes formation and study of AP interactions with biomolecules the ESI is defined as the most effective technique.","PeriodicalId":127734,"journal":{"name":"49","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139362085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of IL-8 and IL-10 Levels in men with Irritable Bowel Syndrome","authors":"Suad M. Salman, Heba T. Yser","doi":"10.56714/bjrs.49.1.3","DOIUrl":"https://doi.org/10.56714/bjrs.49.1.3","url":null,"abstract":"Irritable Bowel Syndrome (IBS) is a common disorder of the large intestine. This study was designed to Estimation of interleukin 8 and 10 in human suffering from irritable bowel syndrome and the relationship with age and period of disease. A total of 88 men participated in this study, including 57 men as patient, and 31 as healthy, who were collected from Al-Fayhaa Hospital in Basrah. The patients was classified according to age into (30-40), (41-50) and (51-60), and according to the disease period into (1-5) and (6-10) years. The healthy were aged (30-60 years).IL-8 and IL-10 were measured using ELISA technique and The data statistical analysis software (One-way Anova) and Mann-Watny test. The results showed a significant increase of interleukin-8 (IL-8) for patients at P≤0.001, while there was a significant decrease in the level of interleukin-10 (IL-10) at P≤0.001. Regarding the age groups, the results did not show variation of IL-8 levels .while, the level of IL-10 in the (41-50 years) was significantly lower. As for the relationship between the duration of the disease there is no significant differences. The study concluded that inflammations play a role in inducing irritable bowel syndrome and the age group (41-50) was more affected.","PeriodicalId":127734,"journal":{"name":"49","volume":"412 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123142775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Genetic Algorithm for DNA Profile Matching","authors":"Nawal S. Jabir1, Zainab A. Kahlaf","doi":"10.56714/bjrs.49.1.2","DOIUrl":"https://doi.org/10.56714/bjrs.49.1.2","url":null,"abstract":"The DNA is used in forensic investigations to identify suspects and victims at crime scenes. However, manual matching of DNA profiles is difficult and error-prone, especially in large databases. In Iraq, technology for DNA matching is limited, making manual matching the only option. Regenerate. In this work, we propose a Genetic Algorithm (GA) for DNA dataset matching to provide simple and user-friendly software to be used by law enforcement agencies in Iraq. The genetic algorithm is a type of heuristic search method used in computing science and artificial intelligence. It is based on the theory of natural selection and evolutionary biology and is used to find the best solutions to search problems. Genetic algorithm is robust for searching through big, complicated datasets. Thus, in this paper, the GA is the algorithm of choice to achieve the goal of DNA matching search. The used dataset is actual data that have been collected from the Ministry of Interior at the Basra Investigation Center. Finally, the python simulation results show 100% accuracy where the proposed method managed to find the DNAs under consideration precisely.","PeriodicalId":127734,"journal":{"name":"49","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116031249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Internet of things usage models: A review and taxonomy","authors":"H. S. Hashim, Z. Hassan, Sulfeeza Bte Mohd Drus","doi":"10.56714/bjrs.49.1.11","DOIUrl":"https://doi.org/10.56714/bjrs.49.1.11","url":null,"abstract":"Academicians “are interested in researching the factors that influence users' acceptance or rejection of IoT technology due to they recognize that understanding the needs and acceptance of individuals is the first step in any collaborative activities from a central area. Many frameworks and models have been created to explain how users usage IoT technology, and these models include factors that may have an impact on user acceptability. An overview of ideas and concepts relating to user acceptability of IoT technology has been presented in this study. The literature that attempts to demonstrate how developers and academics foretell the level of usage IoT will achieve will be given special emphasis in the current review.This study analyzes technology adoption models pertinent to information systems and information technology studies on cutting-edge technologies like the Internet of Things. The study may be used to analyze the adoption and use of new technology. Additionally, it can be applied to enhance these theories and models when implementing new technology.”","PeriodicalId":127734,"journal":{"name":"49","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116061706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the Performance of Searchable Symmetric Encryption by Optimizing Locality","authors":"A. Alyousif, A. Yassin, Z. Abduljabbar, Ke Xu","doi":"10.56714/bjrs.49.1.9","DOIUrl":"https://doi.org/10.56714/bjrs.49.1.9","url":null,"abstract":"Both individuals and organizations prioritize data security and privacy, leading to an increasing focus on technological solutions that emphasize these aspects. Searchable symmetric encryption stands out as a prominent choice for secure storage and search of encrypted data on cloud servers, contributing to this objective. While searchable symmetric encryption offers numerous benefits, it also faces certain challenges, particularly when dealing with large databases. One significant challenge is poor performance, often attributed to poor locality. Visiting multiple locations can significantly increase the time required for data retrieval. Additionally, optimization methods aimed at improving locality can sometimes impact read efficiency or result in excessive storage of the encrypted index on the cloud server. In this paper, we present a scheme that effectively addresses these issues. We have enhanced the encrypted inverted index storage mechanism to improve information retrieval performance. Our scheme achieves optimal locality O(1) and optimal read efficiency in O(1), resulting in a significant increase in retrieval speed. Through experimentation with real-world data, we have demonstrated the practicality, accuracy, and security of our scheme.","PeriodicalId":127734,"journal":{"name":"49","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130996527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer activity of lavender oil and Newcastle disease virus on human glioblastoma: an in vitro study","authors":"Zainab K. Shaheen, Ali A. Al-Ali, Sarmad A. Al-Asadi","doi":"10.56714/bjrs.49.1.1","DOIUrl":"https://doi.org/10.56714/bjrs.49.1.1","url":null,"abstract":"Glioblastoma is one of the most prevalent brain tumors and was the leading cause of cancer death. Since this cancer is an aggressive, incredibly invasive, and neurologically debilitating tumor that does not respond well to chemotherapy, a group of researchers looked at alternative, more efficient therapies. The current study focused on using biological agents and their products (Lavender essential oil and Newcastle disease virus(NDV)) to test their toxicity against the human cerebral glioblastoma (AMGM-5) as a cancer cell model by 3-(4, 5- dimethylthiazol-2-yl-2-) 2.5- diphenyltetrazolium bromide (MTT) viability assay. In brief, the cells were exposed to several concentrations of lavender oil and serial multiplicity of infection (MOI) of NDV for 72 hours. The result shows significantly decreased cell viability treated with lavender oil and NDV in a concentration-dependent manner. The inhibitory concentration that kills 50% of the cells (IC50) of lavender oil was 4.471µg/ml, and the IC50 value of NDV was 0.00473MOI. Also, the morphological study was conducted by the traditional method by using hematoxylin & eosin (H&E) and apoptotic assay by using Acridine orange/Ethidium bromide (AO/EB), the changes are apoptosis and degeneration, as well as cell the emergence of necrotic cells. The compounds of lavender oil were probably responsible for the cytotoxicity of lavender oil. Our results revealed the antagonistic effect of combined essential oil and NDV. Essential oil and NDV have the same toxicity potential for glioblastoma.","PeriodicalId":127734,"journal":{"name":"49","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128860494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of gamma-irradiation on absorption spectrum of fluorescein dye","authors":"R. Abdullah, R. Abul-Hail, H. Badran","doi":"10.56714/bjrs.49.1.12","DOIUrl":"https://doi.org/10.56714/bjrs.49.1.12","url":null,"abstract":"In this study, the fluorescein dye was dissolved in the solvent Dimethyl Form amide (DMF). Weighed equivalent to 0.0664, 0.0996 and 0.132 gm from the material. Using 5mM of solvent, each weight of the substance was dissolved separately to obtain the concentration 60 and 80 mM respectively. The effects of both concentration and gamma radiation on optical spectrum of all samples were investigated at room temperature. After irradiation and within the different concentration percentage at fix dose (4.7 KGy), where the results showed increase absorbance with increase concentration (40, 60 and 80 mM). Also, it is found that the decrease absorbance spectrums at wavelength 460 and 487 nm with increase dose and the good stability absorbance spectrum with time. Because of this, the structures in question are regarded as an efficient material for carrying out real-time gamma radiation dosimetry at ambient temperature.","PeriodicalId":127734,"journal":{"name":"49","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115043651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}