Genes & genetic systems最新文献_第10页

A new allele of engrailed, enNK14, causes supernumerary spermathecae in Drosophila melanogaster. 在黑腹果蝇中，一个新的enk14等位基因导致了多精。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-03-09 DOI: 10.1266/ggs.21-00030

Yasuko Kato, Akiko Sawada, Kazuki Tonai, Hisashi Tatsuno, T. Uenoyama, M. Itoh

{"title":"A new allele of engrailed, enNK14, causes supernumerary spermathecae in Drosophila melanogaster.","authors":"Yasuko Kato, Akiko Sawada, Kazuki Tonai, Hisashi Tatsuno, T. Uenoyama, M. Itoh","doi":"10.1266/ggs.21-00030","DOIUrl":"https://doi.org/10.1266/ggs.21-00030","url":null,"abstract":"A spontaneous mutation, enNK14, was a new allele of engrailed (en) in Drosophila melanogaster. Females of enNK14 have three spermathecae, instead of two in wild type, under a wide range of developmental temperatures, while the males show no abnormal phenotype. Spermathecae of the mutant female can accept inseminated sperms, albeit with a delay of at least an hour until full acceptance compared with wild type. The time course of decrease in the number of stored sperms was thoroughly similar between the mutant and wild type. enNK14 females produced fewer progeny than wild type females despite storing a larger number of sperms. The delay of sperm entry and lower fecundity suggested some functional defects in secretory products of the spermathecae. In addition, some spermathecae in the mutant were accompanied by a mass of brown pigments in the adipose tissue surrounding the capsule. Six contiguous amino acids, Ser340-Ala345, were replaced by one Thr in enNK14. In another mutant, enspt, Ser325 was also shown to be substituted by a Cys. These amino acid changes were located within a serine-rich region, in which Ser325, Ser340 and Thr341 were suggested as targets of Protein Kinase C by an in silico analysis. The splicing pattern of en mRNA did not differ between enNK14 and wild type in embryo, larva, pupa or adult. Our results suggest that en plays an important role in determining the number of spermathecae as well as in sperm storage function in the Drosophila female.","PeriodicalId":12690,"journal":{"name":"Genes & genetic systems","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2022-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47283832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity and population structure of the long-tailed hamster Cricetulus longicaudatus in Shanxi Province, China. 山西省长尾仓鼠遗传多样性及种群结构研究

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-02-23 Epub Date: 2022-01-09 DOI: 10.1266/ggs.20-00060

Xin'gen Yang, Tinglin Wang, Hongfang Guo, Jing Yang, Bo Zou, Jianzhen Zhang

{"title":"Genetic diversity and population structure of the long-tailed hamster Cricetulus longicaudatus in Shanxi Province, China.","authors":"Xin'gen Yang, Tinglin Wang, Hongfang Guo, Jing Yang, Bo Zou, Jianzhen Zhang","doi":"10.1266/ggs.20-00060","DOIUrl":"https://doi.org/10.1266/ggs.20-00060","url":null,"abstract":"The long-tailed hamster Cricetulus longicaudatus is a dominant rodent in farmland of Shanxi Province, China, but little is known about its genetic diversity and population structure. In this study, the genomic DNAs of individuals from 13 populations captured in different fields of Shanxi were extracted and amplified by six pairs of microsatellite primers and by universal primers for mtDNA COI gene sequences. Our data revealed significant departure from Hardy-Weinberg equilibrium in four of the 13 populations. In all 13 populations, the mean observed heterozygosity was significantly lower than the expected heterozygosity. Meanwhile, the mean inbreeding coefficient was statistically significant, which indicated non-random mating within populations. The pairwise genetic distance and natural logarithm of linear geographical distance were not significantly correlated for any C. longicaudatus populations. However, the correlation between genetic distance and resistance distance based on mountain landscape was significant, suggesting that the mountain landscape is an important factor affecting gene flow of C. longicaudatus. Pairwise FST analysis of population structure showed moderate to high genetic differentiation among populations, and all individuals could be divided into two gene clusters. Phylogenetic analysis based on COI sequences also showed that many individuals originated from a single haplotype, suggesting the existence of gene exchange among these populations at some time in the past. Our research should provide a scientific basis for the analysis of genetic differentiation and gene flow among populations of C. longicaudatus.","PeriodicalId":12690,"journal":{"name":"Genes & genetic systems","volume":"96 5","pages":"237-246"},"PeriodicalIF":1.1,"publicationDate":"2022-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39685139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for quick identification of the Japanese salamander Hynobius tokyoensis. 环介导等温扩增(LAMP)快速鉴定东京火蜥蜴方法的建立与评价。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-02-23 Epub Date: 2021-11-07 DOI: 10.1266/ggs.21-00046

Tomoya Suzuki, Kanto Nishikawa, Yukuto Sato, Mamoru Toda

{"title":"Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for quick identification of the Japanese salamander Hynobius tokyoensis.","authors":"Tomoya Suzuki, Kanto Nishikawa, Yukuto Sato, Mamoru Toda","doi":"10.1266/ggs.21-00046","DOIUrl":"https://doi.org/10.1266/ggs.21-00046","url":null,"abstract":"Species identification using molecular techniques has recently become common for various taxa. Loop-mediated isothermal amplification (LAMP) is one of the easiest and least expensive molecular identification methods. Although few studies have developed LAMP assays for amphibians, we believe that LAMP is also useful for identifying endangered amphibians. Hynobius tokyoensis and H. lichenatus occur in Honshu, Japan, and have parapatric distributions. They are similar morphologically, especially at early developmental stages, including eggs and larvae. Hynobius tokyoensis has been listed as a national endangered species in Japan since 2020, and unambiguous identification of these species is therefore important for their conservation and management. In this study, we developed a LAMP primer set for the mitochondrial cytochrome b region to detect H. tokyoensis, and we evaluated the LAMP assay using total genomic DNA from four H. tokyoensis and three H. lichenatus individuals from across most of their ranges. Our LAMP primer set could distinguish these two species. This study should help to establish LAMP assays for other endangered species and morphologically similar species.","PeriodicalId":12690,"journal":{"name":"Genes & genetic systems","volume":"96 5","pages":"247-252"},"PeriodicalIF":1.1,"publicationDate":"2022-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39685854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development and characterization of microsatellite markers for Rhododendron purdomii (Ericaceae) using next-generation sequencing. 利用新一代测序技术开发和鉴定杜鹃花微卫星标记。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-02-23 Epub Date: 2021-12-15 DOI: 10.1266/ggs.21-00031

Ningning Zhang, Mengyun Qin, Shixin Zhu, Ziyang Huang, Hao Dong, Yang Yang, Lili Yang, Yang Lu

{"title":"Development and characterization of microsatellite markers for Rhododendron purdomii (Ericaceae) using next-generation sequencing.","authors":"Ningning Zhang, Mengyun Qin, Shixin Zhu, Ziyang Huang, Hao Dong, Yang Yang, Lili Yang, Yang Lu","doi":"10.1266/ggs.21-00031","DOIUrl":"https://doi.org/10.1266/ggs.21-00031","url":null,"abstract":"Rhododendron purdomii (Ericaceae) is an endangered ornamental species endemic to the Qinling Mountains of China. Due to the impact of climate change and human disturbance, R. purdomii is threatened by habitat loss, and conservation of this species is urgently needed. In this study, we developed and characterized 13 novel microsatellite markers for R. purdomii based on next-generation sequencing data. For the 13 microsatellite markers in three R. purdomii populations, the number of alleles ranged from two to 12, the number of effective alleles was from 1.000 to 8.892, Shannon's information index was from 0.000 to 2.320, and the observed and expected heterozygosity were from 0.000 to 1.000 and from 0.000 to 0.888, respectively. Private alleles were found in all three populations. Moderate differentiation between population pairs was indicated by pairwise FST values. The microsatellite markers developed in this study will provide opportunities for examining the genetic diversity and population structure of R. purdomii and contribute to the effective conservation of this species.","PeriodicalId":12690,"journal":{"name":"Genes & genetic systems","volume":"96 5","pages":"253-257"},"PeriodicalIF":1.1,"publicationDate":"2022-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39841107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Silencing and anti-silencing mechanisms that shape the epigenome in plants. 形成植物表观基因组的沉默和反沉默机制。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-02-23 Epub Date: 2021-10-29 DOI: 10.1266/ggs.21-00041

Soichi Inagaki

引用次数: 2

Heat memory in plants: histone modifications, nucleosome positioning and miRNA accumulation alter heat memory gene expression. 植物的热记忆:组蛋白修饰、核小体定位和miRNA积累改变热记忆基因的表达。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-02-23 Epub Date: 2021-09-16 DOI: 10.1266/ggs.21-00040

Nobutoshi Yamaguchi

{"title":"Heat memory in plants: histone modifications, nucleosome positioning and miRNA accumulation alter heat memory gene expression.","authors":"Nobutoshi Yamaguchi","doi":"10.1266/ggs.21-00040","DOIUrl":"https://doi.org/10.1266/ggs.21-00040","url":null,"abstract":"Plant adaptation to high temperature, often referred to as heat acclimation, is a process in which exposure to moderately high temperatures increases a plant's tolerance to subsequent (normally) lethal high temperatures. Plants store heat experience information (heat memory) obtained from previous exposure to high temperatures for several days and develop future temperature responsiveness. However, our understanding of heat acclimation is very limited. In the model plant Arabidopsis thaliana, changes in the expression patterns of heat memory genes play a central role in regulating plant survival and adaptation to recurring heat stress. Heat stress-related transcription factors and histone-modifying enzymes function in the sensitized expression of heat memory genes via the deposition and removal of histone modifications. Chromatin-remodeling complexes and miRNA accumulation also trigger the sustained expression of heat memory genes. In this review, I describe studies of heat acclimation that have provided important insights into the molecular mechanisms that lead to flexible and reversible gene expression upon heat stress in plants.","PeriodicalId":12690,"journal":{"name":"Genes & genetic systems","volume":"96 5","pages":"229-235"},"PeriodicalIF":1.1,"publicationDate":"2022-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39422220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Spatiotemporal regulation and roles of reproductive phasiRNAs in plants. 植物生殖相rna的时空调控及其作用。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-02-23 Epub Date: 2021-11-11 DOI: 10.1266/ggs.21-00042

Reina Komiya

引用次数: 4

The regulatory mechanisms that make mature sperm cells in the mouse. 小鼠成熟精子细胞产生的调控机制。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-02-01 DOI: 10.1266/ggs.97.1

K. Ichiyanagi

{"title":"The regulatory mechanisms that make mature sperm cells in the mouse.","authors":"K. Ichiyanagi","doi":"10.1266/ggs.97.1","DOIUrl":"https://doi.org/10.1266/ggs.97.1","url":null,"abstract":"Copyright: ©2022 The Author(s). This is an open access article distributed under the terms of the Creative Commons BY 4.0 International (Attribution) License (https://creativecommons.org/ licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited. In multicellular organisms that reproduce sexually, gametes are the sole type of cells that convey genetic information to the next generation. In mice, the germ cell lineage appears at the epiblast stage of embryogenesis, and is separated from the somatic lineage. The emerging germ cells are called primordial germ cells or PGCs, where methylation in the genomic DNA eventually becomes almost completely erased, transiently making their epigenome profoundly different from that of the somatic cells. Then, in sperm cell precursors called prospermatogonia in embryonic testes, de novo DNA methylation takes place genome-wide. These cells subsequently become spermatogonia with the stem cell activity to proliferate in response to external signals, which underlies the life-long production of spermatozoa. Spermatogonia are unipotent stem cells, and a fraction of them differentiate into spermatocytes, in which the transcriptomic program is switched to execute the special cell division process called meiosis. Meiosis in males produces haploid spermatids, which then differentiate into spermatozoa having specific morphology and highly condensed chromatin. All of these consecutive processes are orchestrated by a number of genes, and a failure in any of them results in infertility. In this issue, we have four review articles written by leading young scientists in mammalian germ cell biology. Kenjiro Shirane describes how DNA methylation The regulatory mechanisms that make mature sperm cells in the mouse","PeriodicalId":12690,"journal":{"name":"Genes & genetic systems","volume":"97 1 1","pages":"1"},"PeriodicalIF":1.1,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43743535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subject Index (vol. 97, 2022). 主题索引(卷97,2022)。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-01-01 DOI: 10.1266/ggs.97.325

引用次数: 0

GGS Prize 2022. 2022年GGS奖。

IF 1.1 4区生物学

Genes & genetic systems Pub Date : 2022-01-01 DOI: 10.1266/ggs.97.219

引用次数: 0