复旦学报(医学版)最新文献_第2页

Biliary tract infection and risk factors of patients with malignant obstructive jaundice 恶性梗阻性黄疸患者胆道感染及危险因素分析

Fudan University Journal of Medical Sciences Pub Date : 2017-07-25 DOI: 10.3969/J.ISSN.1672-8467.2017.04.016

Qingmin Yang, Wentao Li

引用次数: 1

A clinical pathologic discussion of multiple cysts of pancreas, intermittent abdominal discomfort 胰腺多发囊肿、间歇性腹部不适的临床病理探讨

Fudan University Journal of Medical Sciences Pub Date : 2017-07-25 DOI: 10.3969/J.ISSN.1672-8467.2017.04.024

H. Gao, K. Jin, Xiao Hong Wang, Zhi Chen, R. Lu, Dan Huang, Xianjun Yu

引用次数: 0

Distinct mutations of MMR gene in colorectal cancer by targeted enrichment and high-throughput next generation sequencing 通过靶向富集和高通量下一代测序研究结直肠癌中MMR基因的不同突变

Fudan University Journal of Medical Sciences Pub Date : 2016-03-25 DOI: 10.3969/J.ISSN.1672-8467.2016.02.014

Kai Huang, Huijing Chen, Fangqi Liu, Ye Xu, X. Li, P. Nan

引用次数: 0

Patients with pelvic serous cancer have more side population(SP)cells and aldehyde dehydrogenase positive(ALDH~+)cells in ascites than in primary foci 盆腔浆液性癌患者腹水侧群(SP)细胞和醛脱氢酶阳性(ALDH~+)细胞多于原发灶

Fudan University Journal of Medical Sciences Pub Date : 2014-11-01 DOI: 10.3969/J.ISSN.1672-8467.2014.06.003

Xiaoxi Ma, Yi Li, Xianrong Zhou, R. Zang, Xiao Xi Sun, Hua Jiang

{"title":"Patients with pelvic serous cancer have more side population(SP)cells and aldehyde dehydrogenase positive(ALDH~+)cells in ascites than in primary foci","authors":"Xiaoxi Ma, Yi Li, Xianrong Zhou, R. Zang, Xiao Xi Sun, Hua Jiang","doi":"10.3969/J.ISSN.1672-8467.2014.06.003","DOIUrl":"https://doi.org/10.3969/J.ISSN.1672-8467.2014.06.003","url":null,"abstract":"Objective To investigate the relationship between pelvic serous cancer stemlike cells(CSC)and peritoneal metastasis. Methods Primary pelvic serous cancer tissues and ascites were obtained from 17 female patients without preoperative chemotherapy from July 2011 to July 2013.Side population(SP)cells and aldehyde dehydrogenase positive(ALDH+)cells were tested by Hoechst33342 staining and ALDH staining respectively to observe their expression difference and their relationship with pelvic metastasis. Results Among the detectable specimen,73.3%(11/15)were detected SP cells positive(percentage of SP cells0.15%),and 100%(10/10)ALDH+cells in either primary foci or ascites.Comparing the cells percentage from different sites in the same patients,73.3%(11/15)of the patients showed higher SP cells in ascites than those in primary tumors(P=0.01),whereas 90%(9/10)of the patiens showed higher ALDH+cells in ascites than those in primary tumors(P=003).Furthermore,patients with both SP positive and more ALDH+cells in ascites showed more metastatic sites than those with only SP positive or ALDH+cells(P0.05),however,they had little difference in metaststic rate(P0.05). Conclusions Higher percentage of SP cells and ALDH+cells in ascites indicates that pelvic serous CSC are related with peritoneal metastasis and disease progression.","PeriodicalId":12635,"journal":{"name":"Fudan University Journal of Medical Sciences","volume":"41 1","pages":"727-733"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70185182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between moesin expression and breast cancer metastasis moesin表达与乳腺癌转移的关系

Fudan University Journal of Medical Sciences Pub Date : 2013-11-01 DOI: 10.3969/J.ISSN.1672-8467.2013.06.009

X. Ni, Z. Shao

{"title":"Relationship between moesin expression and breast cancer metastasis","authors":"X. Ni, Z. Shao","doi":"10.3969/J.ISSN.1672-8467.2013.06.009","DOIUrl":"https://doi.org/10.3969/J.ISSN.1672-8467.2013.06.009","url":null,"abstract":"Objective To explore the internal relationship between moesin expression and breast cancer metastasis. Methods Immunohistochemistry(IHC),RT-PCR and Western blot were`used to identify moesin expression in breast cancer tissue samples and a series of human breast cancer cell lines with different metastatic potentials.RNAi technique combined with invasion and motility assay in vitro were also used to reveal the interrelationship between moesin deficiency and invasion and motility ability of breast cancer cells.Results IHC analysis showed that moesin were mainly distributed on the cell membrane and cytoplasm.Its expression was higher in metastatic breast cancer tissue samples than in non-metastatic ones(P=0.001,χ2=17.536).Western blot results revealed positive correlation between moesin expression and metastatic potential of breast cancer cells.The results of Transwell experiment displayed that moesin expression silencing remarkably inhibited invasion and motility ability of breast cancer cells,especially the cells with high metastatic potential.After moesin expression,the levels of MTDH mRNA and nm-23mRNA in breast cancer cells(P0.05)significantly decreased,while vimentin expression increased,and the expressions of E-cadherin and MMP-9decreased. Conclusions Moesin over-expression presents a positive correlation to breast cancer metastatic potential.Moesin may participate in the process of invasion and metastasis of breast cancer cells through influencing MTDH and nm-23,enhancing epithelial mesenchymal transition and increasing MMP-9secretion.","PeriodicalId":12635,"journal":{"name":"Fudan University Journal of Medical Sciences","volume":"40 1","pages":"679-684"},"PeriodicalIF":0.0,"publicationDate":"2013-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70185078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Anti-cancer effects of trypsin inhibitor targeting glucose-regulated protein 78 (GRP78) in colorectal cancer cells 靶向葡萄糖调节蛋白78 (GRP78)的胰蛋白酶抑制剂在结直肠癌细胞中的抗癌作用

Fudan University Journal of Medical Sciences Pub Date : 2013-03-01 DOI: 10.3969/J.ISSN.1672-8467.2013.02.004

Chao Zhao, Zongrui Li, Rong Fu, Ya Rui Zhao, Tonglin Shi, Zhuo-yu Li

引用次数: 0

Wnt signaling pathway regulates the expression and membrane translocation of GRP78 Wnt信号通路调控GRP78的表达和膜易位

Fudan University Journal of Medical Sciences Pub Date : 2012-09-01 DOI: 10.3969/J.ISSN.1672-8467.2012.05.007

Ya Rui Zhao, Zongrui Li, Y. Zhao, Chao Zhao, Rong Fu, Hanqing Li, Xingchen Wang, Zhuo-yu Li

引用次数: 0