Genes & development最新文献

Corrigendum: Ferredoxin reductase is critical for p53-dependent tumor suppression via iron regulatory protein 2 勘误：铁氧还蛋白还原酶通过铁调节蛋白2对p53依赖性肿瘤抑制至关重要

IF 10.5 1区生物学

Genes & development Pub Date : 2025-10-01 DOI: 10.1101/gad.353221.125

Yanhong Zhang, Yingjuan Qian, Jin Zhang, Wensheng Yan, Yong-Sam Jung, Mingyi Chen, Eric Huang, Kent Lloyd, Yuyou Duan, Jian Wang, Gang Liu, Xinbin Chen

引用次数: 0

An intrinsically disordered region of Drosha selectively promotes miRNA biogenesis independent of tissue-specific Microprocessor condensates Drosha的一个内在紊乱区域选择性地促进miRNA的生物发生，而不依赖于组织特异性微处理器凝聚物

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-29 DOI: 10.1101/gad.352815.125

Bing Yang, Brian J. Galletta, Rima M. Sakhawala, Nasser M. Rusan, Katherine McJunkin

{"title":"An intrinsically disordered region of Drosha selectively promotes miRNA biogenesis independent of tissue-specific Microprocessor condensates","authors":"Bing Yang, Brian J. Galletta, Rima M. Sakhawala, Nasser M. Rusan, Katherine McJunkin","doi":"10.1101/gad.352815.125","DOIUrl":"https://doi.org/10.1101/gad.352815.125","url":null,"abstract":"Precise control of miRNA biogenesis is of extreme importance, because misregulation of miRNAs underlies or exacerbates many disease states. The Microprocessor complex, composed of DROSHA and DGCR8, carries out the first cleavage step in canonical miRNA biogenesis. Despite recent advances in understanding the molecular mechanism of Microprocessor, the N-terminal region of DROSHA is less characterized due to its high intrinsic disorder. Here we demonstrate that Microprocessor forms condensates with properties consistent with liquid–liquid phase separation (LLPS) in select tissues in Caenorhabditis elegans. Although DRSH-1/Drosha recruitment to granules is only partially dependent on its intrinsically disordered regions (IDRs), one of these N-terminal IDRs is crucial for biogenesis of a subset of miRNAs and normal development. A cis region of IDR-dependent miRNAs confers IDR dependence to another miRNA, suggesting that the IDR recognizes sequences or structures in the miRNA primary transcript. Future studies will further elucidate the specificity of this interaction and the putative role of Microprocessor condensates.","PeriodicalId":12591,"journal":{"name":"Genes & development","volume":"45 1","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEF2C networks in heart tube development MEF2C网络在心脏管发展中的应用

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-25 DOI: 10.1101/gad.353315.125

Hassan Abdulrazzak, Mark Mercola

引用次数: 0

The two faces of MyoD: repressor and activator of gene expression during myogenesis MyoD的两面：肌发生过程中基因表达的抑制因子和激活因子

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-22 DOI: 10.1101/gad.353232.125

Carmen Birchmeier

引用次数: 0

Transcription quality control at the promoter-proximal checkpoint 启动子-近端检查点的转录质量控制

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-19 DOI: 10.1101/gad.352973.125

Daniel Blears, Jesper Q. Svejstrup

引用次数: 0

Mice carrying the homologous human shelterin POT1-L259S mutation linked to pulmonary fibrosis show a telomerase deficiency-like phenotype with telomere shortening with increasing mouse generations 携带与肺纤维化相关的同源人类庇护蛋白POT1-L259S突变的小鼠随着小鼠世代的增加，表现出端粒酶缺陷样表型，端粒缩短

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-15 DOI: 10.1101/gad.352855.125

Raúl Sánchez-Vázquez, Sonia Burgaz García-Oteyza, Rosa Serrano, Juana M. Flores, Paula Martínez, Maria A. Blasco

{"title":"Mice carrying the homologous human shelterin POT1-L259S mutation linked to pulmonary fibrosis show a telomerase deficiency-like phenotype with telomere shortening with increasing mouse generations","authors":"Raúl Sánchez-Vázquez, Sonia Burgaz García-Oteyza, Rosa Serrano, Juana M. Flores, Paula Martínez, Maria A. Blasco","doi":"10.1101/gad.352855.125","DOIUrl":"https://doi.org/10.1101/gad.352855.125","url":null,"abstract":"Pulmonary fibrosis is a lethal disease associated with damaging insults to the lung and with organismal aging. The presence of short and dysfunctional telomeres has been placed at the origin of this disease in a percentage of both familial and sporadic cases. Recently, a mutation in the telomere-binding protein protection of telomeres 1 in humans (hPOT1), the hPOT1 L259S mutation, was found in families with idiopathic pulmonary fibrosis. Here, we generated a Pot1aL261S knock-in mouse harboring the murine homologous hPOT1 L259S mutation. We found that the homozygous Pot1aL261S mice show shorter telomeres and degenerative pathologies in the intestine, testes, and lungs at old ages, a phenotype that is aggravated with increasing mouse generations, in striking analogy to the telomerase-deficient mouse models. Furthermore, we found that the POT1a-L261S mutant protein binds more strongly to TPP1 and to telomerase and impedes telomerase-dependent telomere lengthening in vivo. We show that telomerase activity at telomeres is reduced in the presence of POT1a-L261S, which behaves as a dominant negative mutant, thus providing a potential mechanism by which Pot1aL261S knock-in mice phenocopy the short telomere phenotype of the telomerase knockout model.","PeriodicalId":12591,"journal":{"name":"Genes & development","volume":"67 1","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Igf2 regulates early postnatal DPP4+ preadipocyte pool expansion Igf2调节出生后早期DPP4+前脂肪细胞池扩张

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-10 DOI: 10.1101/gad.352710.125

Irem Altun, Khanh Ho Diep Vo, Safal Walia, Xiaocheng Yan, Inderjeet Singh, Ruth Karlina, Viktorian Miok, Lingru Kang, Valentin Kenneth Reichenbach, Andreas Israel, Dominik Lutter, Fabiana Perrochi, Siegfried Ussar

引用次数: 0

A critical affinity window for IgSF proteins DIP-α and Dpr10 is required for proper motor neuron arborization IgSF蛋白DIP-α和Dpr10的关键亲和力窗口是运动神经元正常树突化所必需的

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-10 DOI: 10.1101/gad.352936.125

Davys H. Lopez, Kevin D. Rostam, Sumaira Zamurrad, Shuwa Xu, Richard S. Mann

引用次数: 0

Multiple human enhancer RNAs contain long translated open reading frames 多种人类增强子rna包含长翻译的开放阅读框

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-10 DOI: 10.1101/gad.352944.125

Pavel A. Vlasov, Koichi Ogami, Elizabeth Valenzuela, Risa Karakida Kawaguchi, Marko Jovanovic, James L. Manley

{"title":"Multiple human enhancer RNAs contain long translated open reading frames","authors":"Pavel A. Vlasov, Koichi Ogami, Elizabeth Valenzuela, Risa Karakida Kawaguchi, Marko Jovanovic, James L. Manley","doi":"10.1101/gad.352944.125","DOIUrl":"https://doi.org/10.1101/gad.352944.125","url":null,"abstract":"Enhancer RNAs (eRNAs) are transcribed by RNA polymerase IIduring enhancer activation but are typically rapidly degraded in the nucleus. During states of reduced RNA surveillance, however, eRNAs and other similar “noncoding” RNAs (including, e.g., upstream antisense RNAs) are stabilized, and some are exported to the cytoplasm and can even be found on polysomes. Here, we report unexpectedly that ∼12% of human intergenic eRNAs contain long open reading frames (>300 nt), many of which can be actively translated, as determined by ribosome profiling, and produce proteins that accumulate in cells, as shown by mass spectrometry (MS) data. Focusing on the largest of the encoded proteins, which we designated as eORFs, which can be up to ∼45 kDa, we found, remarkably, that most are highly basic, with pIs >11.5. This unusual chemistry reflects a striking overabundance of arginine residues and occurs despite a relative paucity of lysines. Exogenous expression of the 10 largest eORFs revealed that they accumulate stably in cells as full-length proteins, and most localize to the nucleus and associate with chromatin. Identification of interacting proteins by MS suggested possible roles for these proteins in several nuclear processes. The eORFs studied are well conserved among primates, though they are largely absent from other mammals. Notably, several contain human-specific C-terminal extensions and display properties suggestive of de novo gene birth. In summary, we have discovered that a fraction of human eRNAs can function as mRNAs, revealing a new and unexpected role for these transcripts.","PeriodicalId":12591,"journal":{"name":"Genes & development","volume":"31 1","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum: Reducing MYC's transcriptional footprint unveils a good prognostic gene signature in melanoma 勘误：减少MYC的转录足迹揭示了黑色素瘤良好预后的基因特征

IF 10.5 1区生物学

Genes & development Pub Date : 2025-09-01 DOI: 10.1101/gad.352999.125

Mariano F. Zacarías-Fluck, Daniel Massó-Vallés, Fabio Giuntini, Íñigo González-Larreategui, Jastrinjan Kaur, Sílvia Casacuberta-Serra, Toni Jauset, Sandra Martínez-Martín, Génesis Martín-Fernández, Erika Serrano del Pozo, Laia Foradada, Judit Grueso, Lara Nonell, Marie-Eve Beaulieu, Jonathan R. Whitfield, Laura Soucek

引用次数: 0