Genomics, Proteomics & Bioinformatics最新文献_第8页

Gut Microbiome Variation Along A Lifestyle Gradient Reveals Threats Faced by Asian Elephants 生活方式梯度的肠道微生物组变化揭示了亚洲象面临的威胁

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2023-02-01 DOI: 10.1016/j.gpb.2023.04.003

Chengbo Zhang , Zhenghan Lian , Bo Xu , Qingzhong Shen , Mingwei Bao , Zunxi Huang , Hongchen Jiang , Wenjun Li

{"title":"Gut Microbiome Variation Along A Lifestyle Gradient Reveals Threats Faced by Asian Elephants","authors":"Chengbo Zhang , Zhenghan Lian , Bo Xu , Qingzhong Shen , Mingwei Bao , Zunxi Huang , Hongchen Jiang , Wenjun Li","doi":"10.1016/j.gpb.2023.04.003","DOIUrl":"10.1016/j.gpb.2023.04.003","url":null,"abstract":"<div>The gut microbiome is closely related to host nutrition and health. However, the relationships between gut microorganisms and host lifestyle are not well characterized. In the absence of confounding geographic variation, we defined clear patterns of variation in the gut microbiomes of Asian elephants (AEs) in the Wild Elephant Valley, Xishuangbanna, China, along a lifestyle gradient (completely captive, semicaptive, semiwild, and completely wild). A phylogenetic analysis using the 16S rRNA gene sequences highlighted that the microbial diversity decreased as the degree of captivity increased. Furthermore, the results showed that the bacterial taxon WCHB1-41_c was substantially affected by lifestyle variations. qRT-PCR analysis revealed a paucity of genes related to butyrate production in the gut microbiome of AEs with a completely wild lifestyle, which may be due to the increased unfavorable environmental factors. Overall, these results demonstrate the distinct gut microbiome characteristics among AEs with a gradient of lifestyles and provide a basis for designing strategies to improve the well-being or conservation of this important animal species.</div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"21 1","pages":"Pages 150-163"},"PeriodicalIF":9.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9884923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Gut Microbiome in Colorectal Cancer: Clinical Diagnosis and Treatment 癌症大肠癌肠道微生物组的临床诊断与治疗

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2023-02-01 DOI: 10.1016/j.gpb.2022.07.002

Yali Liu, Harry Cheuk-Hay Lau, Wing Yin Cheng, Jun Yu

{"title":"Gut Microbiome in Colorectal Cancer: Clinical Diagnosis and Treatment","authors":"Yali Liu, Harry Cheuk-Hay Lau, Wing Yin Cheng, Jun Yu","doi":"10.1016/j.gpb.2022.07.002","DOIUrl":"10.1016/j.gpb.2022.07.002","url":null,"abstract":"<div>Colorectal cancer (CRC) is one of the most frequently diagnosed cancers and the leading cause of cancer-associated deaths. Epidemiological studies have shown that both genetic and environmental risk factors contribute to the development of CRC. Several metagenomic studies of CRC have identified gut dysbiosis as a fundamental risk factor in the evolution of colorectal malignancy. Although enormous efforts and substantial progresses have been made in understanding the relationship between human gut microbiome and CRC, the precise mechanisms involved remain elusive. Recent data have shown a direct causative role of the gut microbiome in DNA damage, inflammation, and drug resistance in CRC, suggesting that modulation of gut microbiome could act as a powerful tool in CRC prevention and therapy. Here, we provide an overview of the relationship between gut microbiome and CRC, and explore relevant mechanisms of colorectal tumorigenesis. We next highlight the potential of bacterial species as clinical biomarkers, as well as their roles in therapeutic response. Factors limiting the clinical translation of gut microbiome and strategies for resolving current challenges are further discussed.</div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"21 1","pages":"Pages 84-96"},"PeriodicalIF":9.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9939253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Application of Microbiome in Forensics 微生物组在法医学中的应用

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2023-02-01 DOI: 10.1016/j.gpb.2022.07.007

Jun Zhang , Wenli Liu , Halimureti Simayijiang , Ping Hu , Jiangwei Yan

引用次数: 10

Integration of Computational Analysis and Spatial Transcriptomics in Single-cell Studies 计算分析与空间转录组学在单细胞研究中的整合

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2023-02-01 DOI: 10.1016/j.gpb.2022.06.006

Ran Wang , Guangdun Peng , Patrick P.L. Tam , Naihe Jing

引用次数: 9

Comprehensive Analysis of Ubiquitously Expressed Genes in Humans from A Data-driven Perspective 基于数据驱动的人类普遍表达基因综合分析

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2023-02-01 DOI: 10.1016/j.gpb.2021.08.017

Jianlei Gu , Jiawei Dai , Hui Lu , Hongyu Zhao

{"title":"Comprehensive Analysis of Ubiquitously Expressed Genes in Humans from A Data-driven Perspective","authors":"Jianlei Gu , Jiawei Dai , Hui Lu , Hongyu Zhao","doi":"10.1016/j.gpb.2021.08.017","DOIUrl":"10.1016/j.gpb.2021.08.017","url":null,"abstract":"<div>Comprehensive characterization of spatial and temporal gene expression patterns in humans is critical for uncovering the regulatory codes of the human genome and understanding the molecular mechanisms of human diseases. Ubiquitously expressed genes (UEGs) refer to the genes expressed across a majority of, if not all, phenotypic and physiological conditions of an organism. It is known that many human genes are broadly expressed across tissues. However, most previous UEG studies have only focused on providing a list of UEGs without capturing their global expression patterns, thus limiting the potential use of UEG information. In this study, we proposed a novel data-driven framework to leverage the extensive collection of ∼ 40,000 human transcriptomes to derive a list of UEGs and their corresponding global expression patterns, which offers a valuable resource to further characterize human transcriptome. Our results suggest that about half (12,234; 49.01%) of the human genes are expressed in at least 80% of human transcriptomes, and the median size of the human transcriptome is 16,342 genes (65.44%). Through gene clustering, we identified a set of UEGs, named LoVarUEGs, which have stable expression across human transcriptomes and can be used as internal reference genes for expression measurement. To further demonstrate the usefulness of this resource, we evaluated the global expression patterns for 16 previously predicted disallowed genes in islet beta cells and found that seven of these genes showed relatively more varied expression patterns, suggesting that the repression of these genes may not be unique to islet beta cells.</div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"21 1","pages":"Pages 164-176"},"PeriodicalIF":9.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9899356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The First High-quality Reference Genome of Sika Deer Provides Insights into High-tannin Adaptation 梅花鹿的第一个高质量参考基因组为高单宁适应提供了见解

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2023-02-01 DOI: 10.1016/j.gpb.2022.05.008

Xiumei Xing , Cheng Ai , Tianjiao Wang , Yang Li , Huitao Liu , Pengfei Hu , Guiwu Wang , Huamiao Liu , Hongliang Wang , Ranran Zhang , Junjun Zheng , Xiaobo Wang , Lei Wang , Yuxiao Chang , Qian Qian , Jinghua Yu , Lixin Tang , Shigang Wu , Xiujuan Shao , Alun Li , Fuhe Yang

{"title":"The First High-quality Reference Genome of Sika Deer Provides Insights into High-tannin Adaptation","authors":"Xiumei Xing , Cheng Ai , Tianjiao Wang , Yang Li , Huitao Liu , Pengfei Hu , Guiwu Wang , Huamiao Liu , Hongliang Wang , Ranran Zhang , Junjun Zheng , Xiaobo Wang , Lei Wang , Yuxiao Chang , Qian Qian , Jinghua Yu , Lixin Tang , Shigang Wu , Xiujuan Shao , Alun Li , Fuhe Yang","doi":"10.1016/j.gpb.2022.05.008","DOIUrl":"10.1016/j.gpb.2022.05.008","url":null,"abstract":"<div>Sika deer are known to prefer oak leaves, which are rich in tannins and toxic to most mammals; however, the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear. In identifying the mechanism responsible for the tolerance of a highly toxic diet, we have made a major advancement by explaining the genome of sika deer. We generated the first high-quality, chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments. Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food, especially the expansion of the UGT family 2 subfamily B of UGT genes. The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation. Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals.</div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"21 1","pages":"Pages 203-215"},"PeriodicalIF":9.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9885133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Survey on Methods for Predicting Polyadenylation Sites from DNA Sequences, Bulk RNA-seq, and Single-cell RNA-seq 从DNA序列、体RNA-seq和单细胞RNA-seq预测多聚腺苷酸化位点的方法综述

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2023-02-01 DOI: 10.1016/j.gpb.2022.09.005

Wenbin Ye , Qiwei Lian , Congting Ye , Xiaohui Wu

{"title":"A Survey on Methods for Predicting Polyadenylation Sites from DNA Sequences, Bulk RNA-seq, and Single-cell RNA-seq","authors":"Wenbin Ye , Qiwei Lian , Congting Ye , Xiaohui Wu","doi":"10.1016/j.gpb.2022.09.005","DOIUrl":"10.1016/j.gpb.2022.09.005","url":null,"abstract":"<div>Alternative polyadenylation (APA) plays important roles in modulating mRNA stability, translation, and subcellular localization, and contributes extensively to shaping eukaryotic transcriptome complexity and proteome diversity. Identification of poly(A) sites (pAs) on a genome-wide scale is a critical step toward understanding the underlying mechanism of APA-mediated gene regulation. A number of established computational tools have been proposed to predict pAs from diverse genomic data. Here we provided an exhaustive overview of computational approaches for predicting pAs from DNA sequences, bulk RNA sequencing (RNA-seq) data, and single-cell RNA sequencing (scRNA-seq) data. Particularly, we examined several representative tools using bulk RNA-seq and scRNA-seq data from peripheral blood mononuclear cells and put forward operable suggestions on how to assess the reliability of pAs predicted by different tools. We also proposed practical guidelines on choosing appropriate methods applicable to diverse scenarios. Moreover, we discussed in depth the challenges in improving the performance of pA prediction and benchmarking different methods. Additionally, we highlighted outstanding challenges and opportunities using new machine learning and integrative multi-omics techniques, and provided our perspective on how computational methodologies might evolve in the future for non-3′ untranslated region, tissue-specific, cross-species, and single-cell pA prediction.</div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"21 1","pages":"Pages 67-83"},"PeriodicalIF":9.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/97/main.PMC10372920.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9953906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Genomic Variations in the Tea Leafhopper Reveal the Basis of Its Adaptive Evolution 叶蝉的基因组变异揭示了其适应性进化的基础

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2022-12-01 DOI: 10.1016/j.gpb.2022.05.011

Qian Zhao , Longqing Shi , Weiyi He , Jinyu Li , Shijun You , Shuai Chen , Jing Lin , Yibin Wang , Liwen Zhang , Guang Yang , Liette Vasseur , Minsheng You

{"title":"Genomic Variations in the Tea Leafhopper Reveal the Basis of Its Adaptive Evolution","authors":"Qian Zhao , Longqing Shi , Weiyi He , Jinyu Li , Shijun You , Shuai Chen , Jing Lin , Yibin Wang , Liwen Zhang , Guang Yang , Liette Vasseur , Minsheng You","doi":"10.1016/j.gpb.2022.05.011","DOIUrl":"10.1016/j.gpb.2022.05.011","url":null,"abstract":"<div>Tea green leafhopper (TGL), Empoasca onukii, is of biological and economic interest. Despite numerous studies, the mechanisms underlying its adaptation and evolution remain enigmatic. Here, we use previously untapped genome and population genetics approaches to examine how the pest adapted to different environmental variables and thus has expanded geographically. We complete a chromosome-level assembly and annotation of the E. onukii genome, showing notable expansions of gene families associated with adaptation to chemoreception and detoxification. Genomic signals indicating balancing selection highlight metabolic pathways involved in adaptation to a wide range of tea varieties grown across ecologically diverse regions. Patterns of genetic variations among 54 E. onukii samples unveil the population structure and evolutionary history across different tea-growing regions in China. Our results demonstrate that the genomic changes in key pathways, including those linked to metabolism, circadian rhythms, and immune system functions, may underlie the successful spread and adaptation of E. onukii. This work highlights the genetic and molecular basis underlying the evolutionary success of a species with broad economic impacts, and provides insights into insect adaptation to host plants, which will ultimately facilitate more sustainable pest management.</div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"20 6","pages":"Pages 1092-1105"},"PeriodicalIF":9.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9534744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genome Assembly and Population Resequencing Reveal the Geographical Divergence of Shanmei (Rubus corchorifolius) 山眉(Rubus corchorifolius)基因组组装和种群重测序揭示其地理分化

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2022-12-01 DOI: 10.1016/j.gpb.2022.05.003

Yinqing Yang , Kang Zhang , Ya Xiao , Lingkui Zhang , Yile Huang , Xing Li , Shumin Chen , Yansong Peng , Shuhua Yang , Yongbo Liu , Feng Cheng

{"title":"Genome Assembly and Population Resequencing Reveal the Geographical Divergence of Shanmei (Rubus corchorifolius)","authors":"Yinqing Yang , Kang Zhang , Ya Xiao , Lingkui Zhang , Yile Huang , Xing Li , Shumin Chen , Yansong Peng , Shuhua Yang , Yongbo Liu , Feng Cheng","doi":"10.1016/j.gpb.2022.05.003","DOIUrl":"10.1016/j.gpb.2022.05.003","url":null,"abstract":"<div>Rubus corchorifolius (Shanmei or mountain berry, 2n = 14) is widely distributed in China, and its fruits possess high nutritional and medicinal values. Here, we reported a high-quality chromosome-scale genome assembly of Shanmei, with contig size of 215.69 Mb and 26,696 genes. Genome comparison among Rosaceae species showed that Shanmei and Fupenzi (Rubus chingii Hu) were most closely related, followed by blackberry (Rubus occidentalis), and that environmental adaptation-related genes were expanded in the Shanmei genome. Further resequencing of 101 samples of Shanmei collected from four regions in the provinces of Yunnan, Hunan, Jiangxi, and Sichuan in China revealed that among these samples, the Hunan population of Shanmei possessed the highest diversity and represented the more ancestral population. Moreover, the Yunnan population underwent strong selection based on the nucleotide diversity, linkage disequilibrium, and historical effective population size analyses. Furthermore, genes from candidate genomic regions that showed strong divergence were significantly enriched in the flavonoid biosynthesis and plant hormone signal transduction pathways, indicating the genetic basis of adaptation of Shanmei to the local environment. The high-quality assembled genome and the variome dataset of Shanmei provide valuable resources for breeding applications and for elucidating the genome evolution and ecological adaptation of Rubus species.</div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"20 6","pages":"Pages 1106-1118"},"PeriodicalIF":9.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9912840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Revisiting the Evolutionary History of Pigs via De Novo Mutation Rate Estimation in A Three-generation Pedigree 通过对三代家系的从头突变率估计来回顾猪的进化史

IF 9.5 2区生物学

Genomics, Proteomics & Bioinformatics Pub Date : 2022-12-01 DOI: 10.1016/j.gpb.2022.02.001

Mingpeng Zhang, Qiang Yang, Huashui Ai, Lusheng Huang

{"title":"Revisiting the Evolutionary History of Pigs via De Novo Mutation Rate Estimation in A Three-generation Pedigree","authors":"Mingpeng Zhang, Qiang Yang, Huashui Ai, Lusheng Huang","doi":"10.1016/j.gpb.2022.02.001","DOIUrl":"10.1016/j.gpb.2022.02.001","url":null,"abstract":"<div>The mutation rate used in the previous analyses of pig evolution and demographics was cursory and hence invited potential bias in inferring evolutionary history. Herein, we estimated the de novo mutation rate of pigs as 3.6 × 10−9 per base per generation using high-quality whole-genome sequencing data from nine individuals in a three-generation pedigree through stringent filtering and validation. Using this mutation rate, we re-investigated the evolutionary history of pigs. The estimated divergence time of ∼ 10 kiloyears ago (KYA) between European wild and domesticated pigs was consistent with the domestication time of European pigs based on archaeological evidence. However, other divergence events inferred here were not as ancient as previously described. Our estimates suggest that Sus speciation occurred ∼ 1.36 million years ago (MYA); European wild pigs split from Asian wild pigs only ∼ 219 KYA; and south and north Chinese wild pigs split ∼ 25 KYA. Meanwhile, our results showed that the most recent divergence event between Chinese wild and domesticated pigs occurred in the Hetao Plain, northern China, approximately 20 KYA, supporting the possibly independent domestication in northern China along the middle Yellow River. We also found that the maximum effective population size of pigs was ∼ 6 times larger than estimated before. An archaic migration from other Sus species originating ∼ 2 MYA to European pigs was detected during western colonization of pigs, which may affect the accuracy of previous demographic inference. Our de novo mutation rate estimation and its consequences for demographic history inference reasonably provide a new vision regarding the evolutionary history of pigs.</div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"20 6","pages":"Pages 1040-1052"},"PeriodicalIF":9.5,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/af/3f/main.PMC10225487.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9534268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6