General physiology and biophysics最新文献_第4页

Neuroprotective effects of coffee-derived exosome-like nanoparticles against Aβ-induced neurotoxicity. 咖啡外泌体纳米颗粒对Aβ诱导的神经毒性的神经保护作用

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024025

Meric A Esmekaya, Burhan Ertekin

{"title":"Neuroprotective effects of coffee-derived exosome-like nanoparticles against Aβ-induced neurotoxicity.","authors":"Meric A Esmekaya, Burhan Ertekin","doi":"10.4149/gpb_2024025","DOIUrl":"https://doi.org/10.4149/gpb_2024025","url":null,"abstract":"The present study aimed to provide experimental evidence that CDELNs (coffee-derived exosome-like nanoparticles) may be a candidate for the treatment or prevention of amyloid-β (Aβ)-induced Alzheimer's disease (AD). An in vitro Alzheimer's model was created with Aβ-induced toxicity in mouse hippocampal neuronal cells (HT-22). Aβ(1-42)-exposed cells were treated with different concentrations of CDELNs (1-50 μg/ml) and the viability of cells was analyzed. The change in the mitochondrial membrane potential (ΔΨm) of cells was also determined. CDELNs treatment increased the viability of Aβ(1-42 )-toxicity-induced HT-22 cells significantly. The increase in the viability of Aβ(1-42)-toxicity-induced cells was correlated with an improvement in ΔΨm. CDELNs treatment restored the dissipated ΔΨm. These results suggested that CDELNs protect neuronal cells against Aβ(1-42)-induced neurotoxicity by repairing mitochondrial dysfunction. CDELNs might be a useful neuroprotective agent for the treatment or prevention of Aβ-induced AD. Further animal and clinical studies should be carried out to investigate the neuroprotective potential of CDELNs against Aβ-induced AD.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"535-543"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extremely low frequency pulsed magnetic field inhibits myocardial damage and apoptosis in rats with CLP-induced sepsis: A histopathological and immunohistochemical evaluation. 极低频脉冲磁场可抑制 CLP 诱导败血症大鼠的心肌损伤和细胞凋亡：组织病理学和免疫组织化学评估。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024029

Serkan Gürgül, Fikret Gevrek, Serkan Yelli, Fatma B Şeker, Can Demirel

{"title":"Extremely low frequency pulsed magnetic field inhibits myocardial damage and apoptosis in rats with CLP-induced sepsis: A histopathological and immunohistochemical evaluation.","authors":"Serkan Gürgül, Fikret Gevrek, Serkan Yelli, Fatma B Şeker, Can Demirel","doi":"10.4149/gpb_2024029","DOIUrl":"https://doi.org/10.4149/gpb_2024029","url":null,"abstract":"We aimed to investigate whether pulsed magnetic fields (PMFs) (1 mT) may have preventive effects on myocardial damage and apoptosis in rats with sepsis. Twenty-eight adult Wistar albino rats were evenly distributed among four experimental groups, each consisting of seven rats: SH, LF-PMF, HF-PMF, and CLP. Sepsis induction was carried out via the cecal ligation and puncture (CLP) method, while rats in the LF-PMF and HF-PMF groups were exposed to 7.5 Hz and 15 Hz PMF, respectively, for duration of 24 hours. Following the removal of heart tissue, histological techniques were employed for the analysis. Histological scoring of apoptosis-related Bax, Bcl-2, and Acas-3 proteins as well as cTnI were performed in the heart tissue. The myocardial damage score significantly increased in the CLP group compared to the SH group (p < 0.05). Significant decreases were observed in Bcl-2 and cTnI protein levels in the CLP group, while significant increases were detected in the PMF groups (p < 0.05). An increase in Bax and Acas-3 protein levels, as well as the Bax/Bcl-2 ratio, was observed in the CLP group, with a decrease in the PMF groups (p < 0.05). The results demonstrate that PMF application has anti-apoptotic and therapeutic effects on septic heart tissue damage.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"555-566"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HMGB1 impacts the intestinal epithelial barrier by initiating NETs to regulate macrophage polarization. HMGB1 通过启动 NET 来调节巨噬细胞的极化，从而影响肠上皮屏障。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-11-01 DOI: 10.4149/gpb_2024034

Xiaohong Chen, Junyi Wu, Meng Liu, Zheng Han, Jie Tan, Qingxi Zhu, Xiaodong Huang, Xia Tian

{"title":"HMGB1 impacts the intestinal epithelial barrier by initiating NETs to regulate macrophage polarization.","authors":"Xiaohong Chen, Junyi Wu, Meng Liu, Zheng Han, Jie Tan, Qingxi Zhu, Xiaodong Huang, Xia Tian","doi":"10.4149/gpb_2024034","DOIUrl":"https://doi.org/10.4149/gpb_2024034","url":null,"abstract":"High mobility group box 1 (HMGB1) has the capability of activating the immune response and taking part in macrophage polarization. Despite this, there is significant scope for exploration into how HMGB1 regulates macrophage polarization phenotype and influences intestinal epithelial barrier function. Investigating the role of HMGB1 in the creation of neutrophil extracellular traps (NETs) and the mechanism of its impact on macrophages could provide novel insights into intervening in intestinal inflammation and barrier damage. Therefore, the research examined the relationship between the macrophage polarization phenotype and HMGB1. Additionally, we analyzed how cell proliferation and cytokines changed in CaCo-2 cells following co-culture with HMGB1-influenced macrophages and intestinal epithelial CaCo-2 cells. We discovered that up-regulation of HMGB1 expression enhanced the creation of NETs, whereas inhibition of NETs formation led macrophages to switch from the anti-inflammatory M2 phenotype to the pro-inflammatory M1 phenotype. Additionally, we observed that macrophages induced by NETs containing HMGB1 can prompt CaCo-2 cell apoptosis and exacerbate the inflammatory response. HMGB1-containing NETs hinder tight junction protein expression in CaCo-2 cells by inducing macrophage M1 polarization, thereby impairing intestinal epithelial barrier function. Therefore, our findings indicate that by inhibiting the expression of HMGB1, the formation of NETs can be inhibited. This, in turn, mediates macrophage polarization and offers potential new therapies for intestinal diseases.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 6","pages":"545-554"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Down-regulated miR-10a protects against spinal cord injury by up-regulating SIRT1 COVID-19 与口面裂隙之间的关系。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-09-01 DOI: 10.4149/gpb_2024024

Chao Song, Yan Zhang

{"title":"Down-regulated miR-10a protects against spinal cord injury by up-regulating SIRT1","authors":"Chao Song, Yan Zhang","doi":"10.4149/gpb_2024024","DOIUrl":"10.4149/gpb_2024024","url":null,"abstract":"MicroRNAs (miRNAs) are essential modulators of gene expression and are associated with various pathological processes, including spinal cord injury (SCI). This investigation aimed to elucidate miR-10a activity in SCI and its potential interaction with sirtuin 1 (SIRT1). The SCI rat model was established to assess hind limb movement, measure levels of miR-10a, SIRT1, neuronal survival, and inflammatory factors. An in-vitro SCI cell model was also developed to evaluate cell viability and inflammatory factor levels. The interaction between miR10a and SIRT1 was verified. Upregulated miR-10a and downregulated SIRT1 expression were found in the tissues of SCI rats. miR-10a knockdown in SCI rats enhanced the recovery of motor function, increased neuronal survival, and reduced the levels of inflammatory cytokines. Luciferase reporter assays confirmed that miR-10a targeted SIRT1 directly. In PC12 cells, downregulation of miR-10a increased SIRT1 expression, enhanced cell viability, and reduced inflammatory factor levels after LPS stimulation. Conversely, SIRT1 knockdown inhibited the protective effects of downregulated miR-10a on cell viability and inflammatory responses. The results suggest that miR-10a downregulation protects against SCI by upregulating SIRT1 expression, improving functional recovery, and reducing inflammation. Targeting the miR-10a/SIRT1 axis is a promising strategy for SCI treatment.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 5","pages":"435-443"},"PeriodicalIF":1.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression profiles and functional prediction of circular RNAs in lung cancer patients with chronic obstructive pulmonary disease: a pilot study. 慢性阻塞性肺病肺癌患者循环 RNA 的差异表达谱和功能预测：一项试点研究。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-07-01 DOI: 10.4149/gpb_2024013

Xiaoou Li, Yongchun Shen, Jiahan Cheng, Jun Chen, Zhicheng Yuan, Tao Wang, Lei Chen, Lunxu Liu, Fuqiang Wen

{"title":"Differential expression profiles and functional prediction of circular RNAs in lung cancer patients with chronic obstructive pulmonary disease: a pilot study.","authors":"Xiaoou Li, Yongchun Shen, Jiahan Cheng, Jun Chen, Zhicheng Yuan, Tao Wang, Lei Chen, Lunxu Liu, Fuqiang Wen","doi":"10.4149/gpb_2024013","DOIUrl":"https://doi.org/10.4149/gpb_2024013","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD), characterized by clinical sub-phenotypes such as emphysema (E) and chronic bronchitis (CB), is associated with a greater risk of lung cancer (LC). This study aimed to assess the expression patterns of circRNA and their potential functional involvement in LC patients with COPD. A circRNA microarray was used to characterize differentially expressed circRNAs (DEcircRNAs) profiles. A total of 176, 240, 163, and 243 DEcircRNAs were identified in comparisons between CB vs. LC patients (Con), E vs. Con, E vs. CB, and CBE vs. Con, respectively. DEcircRNAs in all comparison groups were primarily associated with immune-related GO terms and were also enriched in immune and inflammatory pathways. In total, 49 DEcircRNAs were significantly correlated with the infiltration of multiple immune cells. Among them, hsa-MROH9_0001 and hsa-RP11-35J10_0013 were positively and negatively correlated with plasma cells and T-cell CD4 memory resting cells, respectively; these two DEcircRNA-sponged miRNAs have good diagnostic performance. WGCNA identified six key circRNAs associated with CB progression. The expression patterns of hsa-MROH9_0001 and circRNA_21729 in E and CB groups were confirmed by RT-qPCR. In conclusion, we reported circRNA profiles and the findings demonstrated that hsa-MROH9_0001 and circRNA_21729 may be potential therapeutic targets for LC with COPD.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 4","pages":"273-289"},"PeriodicalIF":1.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A programming toolbox for calculating beta-Euler shape exponents from plant growth data. 根据植物生长数据计算 beta-Euler 形状指数的编程工具箱。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-07-01 DOI: 10.4149/gpb_2024016

Jerzy Kosek, Mariusz Pietruszka

引用次数: 0

CHORDC1, the novel interacting partner of tau protein. CHORDC1，tau 蛋白的新型相互作用伙伴。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-07-01 DOI: 10.4149/gpb_2024019

Karoline Pichlerová, Jakub Šinský, Matej Kotásek, Petra Majerová, Jozef Hanes

引用次数: 0

Interference with sphingosine kinase-1 reduces the hydrogen peroxide-induced oxidative stress damage in melanocytes through four and a half LIM domains 2. 通过四个半 LIM 结构域 2 干扰鞘氨醇激酶-1，减少过氧化氢诱导的黑色素细胞氧化应激损伤。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-07-01 DOI: 10.4149/gpb_2024011

Kuo-Hsiang Liao, Kuo-Liang Liao

{"title":"Interference with sphingosine kinase-1 reduces the hydrogen peroxide-induced oxidative stress damage in melanocytes through four and a half LIM domains 2.","authors":"Kuo-Hsiang Liao, Kuo-Liang Liao","doi":"10.4149/gpb_2024011","DOIUrl":"10.4149/gpb_2024011","url":null,"abstract":"Vitiligo is featured by manifestation of white maculae and primarily results from oxidative stress. Sphingosine kinase-1 (SPHK1) participates in oxidative stress. This paper was devised to explore the role of SPHK1 in vitiligo and to disclose the mechanism. PIG1 cell viability was appraised utilizing cell counting kit-8 assay while Western blot detected SPHK1 and four and a half LIM domains 2 (FHL2). The transduction efficacy of small interfering RNA (siRNA)-SPHK1, siRNA-FHL2 and pcDNA3.1 plasmid overexpressing FHL2 (Ov-FHL2) was checked using Western blot. Flow cytometry detected cell apoptotisis. Western blot detected mitochondrial cytochrome c (Mit-Cyt-c) and cytosolic cytochrome c (Cyto-Cyt-c). Dichloro-dihydro-fluorescein diacetate (DCFH-DA) detected reactive oxygen species (ROS) activity while oxidative stress markers were evaluated using corresponding assay kits. SPHK1 expression was discovered to be increased in hydrogen peroxide (H2O2)-challenged PIG1 cells and SPHK1 interference alleviated H2O2-challenged viability damage, apoptosis, oxidative stress and FHL2 expression in PIG1 cells. FHL2 depletion could suppress viability damage, apoptosis and oxidative stress in H2O2-challenged PIG1 cells. Rescue experiments demonstrated that the suppressive impacts of SPHK1 deficiency on PIG1 cell viability, apoptosis and oxidative stress induced by H2O2 were offset by FHL2 overexpression. Collectively, SPHK1 knockdown protected against vitiligo via the regulation of FHL2.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 4","pages":"321-333"},"PeriodicalIF":1.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Berberine protects against sepsis-related acute lung injury in rats via PPAR-γ signaling pathway upregulation and improvement at the cellular level: Functional, biochemical, and immunohistochemistry study. 小檗碱通过 PPAR-γ 信号通路的上调和细胞水平的改善保护大鼠免受脓毒症相关急性肺损伤：功能、生化和免疫组化研究。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-07-01 DOI: 10.4149/gpb_2024008

Mohamed M Khalifa, Nermeen A Bastawy, Laila A Rashed, Hanan A Hassan, Omnia M Abdel-Maksoud, Fatma E Hassan

{"title":"Berberine protects against sepsis-related acute lung injury in rats via PPAR-γ signaling pathway upregulation and improvement at the cellular level: Functional, biochemical, and immunohistochemistry study.","authors":"Mohamed M Khalifa, Nermeen A Bastawy, Laila A Rashed, Hanan A Hassan, Omnia M Abdel-Maksoud, Fatma E Hassan","doi":"10.4149/gpb_2024008","DOIUrl":"10.4149/gpb_2024008","url":null,"abstract":"This study aimed to assess the prophylactic effects of Berberine on experimentally induced lung sepsis and examine its effects on selected cytokines, genes, and protein expression besides the histopathological evaluation. Berberine significantly reduced the wet/dry lung ratio, the broncho-alveolar lavage fluid (BALF) protein, cells, neutrophils percentage, and cytokines levels. In addition, pretreatment with Berberine decreased the myeloperoxidase (MPO) and malondialdehyde (MDA) levels and decreased gene expression of nuclear factor kappa B (NF-κB), monocyte chemoattractant protein-1 (MCP-1), and the intracellular adhesion molecule 1 (ICAM-1) by RT-qPCR analysis, revealing Berberine's antioxidant and anti-inflammatory mode of action. Western blot analysis revealed increased peroxisome proliferator-activated receptor gamma (PPAR-γ) expression in the Berberine pretreated group compared to the cecal ligation and puncture (CLP) group, in which the histopathological examination evidenced this improvement. In conclusion, Berberine improved lung sepsis via its PPAR-γ mediated antioxidant and anti-inflammatory effects.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 4","pages":"353-366"},"PeriodicalIF":1.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A2A adenosine receptor stimulation ameliorated diabetic-induced osteoporosis in rats. A2A 腺苷受体刺激可改善糖尿病诱发的大鼠骨质疏松症。

IF 1.3 4区生物学

General physiology and biophysics Pub Date : 2024-07-01 DOI: 10.4149/gpb_2024018

Manal S Abd-El Hamid, Ebtessam A Abou-Shady, Nourhan A Mohamed, Wessam E Morsy

{"title":"A2A adenosine receptor stimulation ameliorated diabetic-induced osteoporosis in rats.","authors":"Manal S Abd-El Hamid, Ebtessam A Abou-Shady, Nourhan A Mohamed, Wessam E Morsy","doi":"10.4149/gpb_2024018","DOIUrl":"10.4149/gpb_2024018","url":null,"abstract":"Diabetic osteoporosis is a common health problem that is associated with a disruption in bone metabolism. A2A adenosine receptor (A2AAR) signaling seems to play a critical role in bone homeostasis. This study aimed to evaluate the effect of A2AAR stimulation on the treatment of diabetic-induced osteoporosis versus insulin treatment. Forty adult male rats were allocated into control (C), untreated diabetic-induced osteoporosis (DIO), insulin-treated DIO (I-DIO), and A2AAR agonist-treated DIO (A-DIO) groups. Both insulin and A2AAR agonist treatments significantly increased serum insulin level, glutathione peroxidase (GPx) activity, bone expression of osteoprotegerin (Opg) and β-catenin (Ctnnb1), and cortical and trabecular bone thickness, whereas they decreased serum fasting glucose, malondialdehyde (MDA), tumor necrosis factor α (TNF-α), bone expression of receptor activator of nuclear factor kappa-B ligand (Rankl), runt-related transcription factor-2 (Runx2), and sclerostin (Sost) versus the untreated DIO groups. A2AAR agonist treatment was more effective than insulin in ameliorating diabetic osteoporosis. This might be attributed to the upregulation of β-catenin gene expression, enhancing its anabolic effect on bone, in addition to the A2AAR agonist's anti-oxidative, anti-inflammatory, and anti-diabetic effects.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"43 4","pages":"335-346"},"PeriodicalIF":1.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0