Juntae Kim, Sung-Hwan Ahn, Suk-Ki Yoon, Hyang-Rae Noh, Hwa-Jin Jeong, Chang-Wan Sun, Byoung-Kwan Cho
{"title":"Development of a smartphone-based bone maturity classification algorithm with XAI for beef carcass grading.","authors":"Juntae Kim, Sung-Hwan Ahn, Suk-Ki Yoon, Hyang-Rae Noh, Hwa-Jin Jeong, Chang-Wan Sun, Byoung-Kwan Cho","doi":"10.1007/s44463-025-00039-4","DOIUrl":"10.1007/s44463-025-00039-4","url":null,"abstract":"<p><p>In Korea, beef carcass quality grades are primarily determined based on the loin-eye muscle area and the degree of marbling. However, final grading decisions also consider additional factors, including carcass abnormalities such as the extent of cartilage ossification. Ossification is classified into nine levels, with lower levels indicating younger cattle. In the case of multiparous cows, which are generally older and exhibit higher ossification levels, a grade reduction is typically applied when the ossification level reaches 8 or 9. Traditionally, ossification levels have been assessed visually by human graders. However, the inherent subjectivity of this method has led to inconsistencies, highlighting the need for more objective, image-based assessment techniques. In this study, ossification levels ranging from 6 to 9 were documented using a smartphone camera, resulting in a dataset of 1,770 images per grade. To evaluate the ossification levels, object detection models including YOLO v8, v9, v10, and v11 were compared. All models achieved an accuracy exceeding 95%, demonstrating their potential for practical application in slaughterhouse environments. In particular, the YOLO v9m and YOLO v10m models showed the highest accuracy of 99.08% and 99.22%, respectively. Furthermore, explainable artificial intelligence (XAI) techniques such as Grad-CAM and LIME were applied to visually confirm that the deep learning models focused on regions relevant to carcass maturity. This study presents an efficient and reliable approach for assessing cartilage ossification using smartphone-based deep learning. The proposed method offers improved grading accuracy and consistency, supporting its adoption in modern meat processing facilities.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12995012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minyoung Jung, Sukyung Kim, Jeongmin Song, Hyun Mi Kim, Yeonghee Kim, Min Hee Lee, Yechan Kyung, Minji Kim, Sanghee Shin, Sehun Jang, Woong Bom Kim, Hyeonwoo Kim, Bomin Koh, Bongsang Kim, Junghyun Lim, Chanwoo Kim, Jaeho Lee, Suk-Joo Choi, Soo-Young Oh, Jihyun Kim, Kangmo Ahn
{"title":"Effects of feeding type on gut microbiota and atopic dermatitis in cesarean delivered infants: a combined birth cohort study.","authors":"Minyoung Jung, Sukyung Kim, Jeongmin Song, Hyun Mi Kim, Yeonghee Kim, Min Hee Lee, Yechan Kyung, Minji Kim, Sanghee Shin, Sehun Jang, Woong Bom Kim, Hyeonwoo Kim, Bomin Koh, Bongsang Kim, Junghyun Lim, Chanwoo Kim, Jaeho Lee, Suk-Joo Choi, Soo-Young Oh, Jihyun Kim, Kangmo Ahn","doi":"10.1007/s44463-025-00040-x","DOIUrl":"10.1007/s44463-025-00040-x","url":null,"abstract":"<p><p>To investigate the effect of early feeding type on gut microbiota composition and atopic dermatitis (AD) development in cesarean-delivered infants, and to evaluate whether probiotic-supplemented formula can mitigate microbial and immunologic disadvantages associated with cesarean delivery. A total of 129 infants delivered by cesarean section were enrolled and classified into three groups: Group A (exclusively breastfed), Group B (fed standard formula), and Group C (fed probiotic-fortified formula). Stool samples were collected at 6–8 weeks of age and analyzed using 16S rRNA gene sequencing. Microbial diversity indices and taxonomic composition were compared, and the incidence of AD by 6 months was assessed. The incidence of AD by 6 months was 8.3% in Group A, 66.7% in Group B, and 25.0% in Group C (p = 0.793). Group A showed the highest gut microbial diversity (Shannon, Chao1, and observed amplicon sequence variants), with significantly lower diversity in Group C (adjusted p < 0.017). No significant differences were observed in the Simpson index. Group C exhibited a significantly higher relative abundance of Bifidobacterium compared with Groups A and B (adjusted p < 0.001), while Lactobacillus was most abundant in Group A, with significant differences across all groups (adjusted p < 0.001). The incidence of AD did not significantly differ between Group A and Group C . While early probiotic supplementation via infant formula may support beneficial bacterial colonization in cesarean-delivered infants, its potential role in AD prevention remains to be clarified through further longitudinal studies.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12995016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hee Yeon Cho, Ji-Eun Lee, Jae Hoon Lee, Dong Uk Ahn, Kee-Tae Kim, Hyun-Dong Paik
{"title":"Improved antioxidant and anti-melanogenic effects of ovalbumin derived from egg white after enzymatic hydrolysis.","authors":"Hee Yeon Cho, Ji-Eun Lee, Jae Hoon Lee, Dong Uk Ahn, Kee-Tae Kim, Hyun-Dong Paik","doi":"10.1007/s44463-025-00024-x","DOIUrl":"10.1007/s44463-025-00024-x","url":null,"abstract":"<p><p>Consumption of oxidized foods or exposure of skin to ultraviolet (UV) can produce reactive oxygen species (ROS) that increase oxidative stresses that can lead to skin aging and melanogenesis. Hydrolyzing proteins like ovalbumin (OA) can significantly increase their bioactivities. In this study, OA was hydrolyzed using Alcalase 2.4 L FG, Multifect PR 14 L, and Papain T100 MG, and the antioxidant and melanogenesis inhibitory activities of the hydrolysates were determined using B16F10 melanoma cells. All OA hydrolysates (OAH) showed higher antioxidant activities than the natural OA. The OAH prepared with Multifect PR 14 L (OAMF) exhibited the highest antioxidant activities and significantly inhibited melanin production and tyrosinase activity in the stimulated B16F10 cells using α-melanocyte-stimulating hormone. The DOPA staining confirmed the melanogenesis inhibitory (melanin formation and dendrite development) effects of OAMF. The OAMF (100, 200, and 400 µg/mL) treatment downregulated the mRNA expression of microphthalmia-associated transcription factor (MITF) and melanogenic enzymes [tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2)] dose-dependently. Therefore, OAMF can help reduce oxidative stress in humans when consumed and can be used as an anti-aging and skin-lightening agent in the cosmetic industry.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12994976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seul-Ki-Chan Jeong, Seonmin Lee, Hayeon Jeon, Seokhee Han, Soeun Kim, Minkyung Woo, Pil Nam Seong, Samooel Jung, Kyung Jo
{"title":"Prediction of pork belly cooking loss by hyperspectral imaging analysis of semispinalis capitis muscle.","authors":"Seul-Ki-Chan Jeong, Seonmin Lee, Hayeon Jeon, Seokhee Han, Soeun Kim, Minkyung Woo, Pil Nam Seong, Samooel Jung, Kyung Jo","doi":"10.1007/s44463-025-00038-5","DOIUrl":"10.1007/s44463-025-00038-5","url":null,"abstract":"<p><p>This study evaluated the performance of a partial least squares regression (PLSR) model in predicting the cooking loss of pork belly slices using hyperspectral images of the semispinalis capitis (SC) muscle from the pork shoulder butt. Pork shoulder butts and whole belly cuts were obtained from 70 half-right gilt carcasses at 24 h postmortem. Pork belly slices were obtained from the 6th and 11th thoracic vertebrae and the 4th lumbar vertebra. Reflectance hyperspectral images of the SC muscle were captured. Cooking loss was measured in SC muscle and pork belly slices. A significant correlation was observed between the cooking loss of pork belly slices and SC muscle. In the PLSR model for predicting the cooking loss of belly slices, standard normalized variate pre-processing and wavelength selection did not enhance the model accuracy. In contrast, first-derivative pre-processing showed the highest accuracy with R<sup>2</sup>v values ranging from 0.73 to 0.80. These results suggest that the cooking loss of pork belly slices can be nondestructively predicted using the hyperspectral image of SC muscle exposed on the half-carcass surface. Moreover, this study can provide a foundation for future research on nondestructive and real-time prediction of pork quality in production lines.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12995054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lingke Wang, Jiayou Li, Yixin Chen, Yang Zhang, Ruyuan Zhang
{"title":"Insect proteins and peptides: preparation, bioactivities, and applications.","authors":"Lingke Wang, Jiayou Li, Yixin Chen, Yang Zhang, Ruyuan Zhang","doi":"10.1007/s44463-025-00013-0","DOIUrl":"10.1007/s44463-025-00013-0","url":null,"abstract":"<p><p>The surge in global population has driven a dramatic increase in protein demand, and insects have emerged as a promising sustainable high-quality protein resource due to their advantages of high reproductive rates, low production costs, high protein content, and balanced nutritional profiles. This review systematically compares the traditional and innovative extraction methods for insect proteins and peptides, and explores their functional characteristics. Moreover, this review summarizes the multiple applications of insect proteins and peptides in food, feed, and pharmaceutical industry, and provides a forward-looking perspective on the challenges that impede their broader implementation, and concrete recommendations are proposed to facilitate the industrial advancement of insect-derived bioproducts and foster their integration into mainstream consumer markets.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12995055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juhyun Lee, Da-Young Lee, Ermie Jr Mariano, Ji Won Park, Seok Namkung, So Young Choi, Woo Jin Lee, Ye Won Shin, Chae Hyeon Bok, Colin Venter, Younsu Lee, Sun Jin Hur
{"title":"Utilization of animal by-products as sources of bioactive compounds and FBS alternatives for cultured meat: a comprehensive review.","authors":"Juhyun Lee, Da-Young Lee, Ermie Jr Mariano, Ji Won Park, Seok Namkung, So Young Choi, Woo Jin Lee, Ye Won Shin, Chae Hyeon Bok, Colin Venter, Younsu Lee, Sun Jin Hur","doi":"10.1007/s44463-025-00035-8","DOIUrl":"10.1007/s44463-025-00035-8","url":null,"abstract":"<p><p>Slaughterhouse by-products have been widely utilized in food, pharmaceutical, and biomaterial applications across Asia, Europe, and the Americas. This review examines current research on enhancing the value and utilization of these by-products, with a particular focus on their potential as alternatives to fetal bovine serum (FBS), a critical component of cell culture media. Among these, livestock and poultry blood-produced at over 30 million tons annually-is of particular interest; however, only approximately 30% is currently used in food products or additives. Recent studies suggest that serum derived from bovine, porcine, and chicken blood may serve as viable substitutes for FBS. Recent studies have suggested that serum derived from adult bovine, porcine, and chicken blood may partially substitute for FBS, with replacement efficiencies reported to reach up to approximately 80%, depending on the species. Inedible by-products, including feathers, bones, leather, and hooves, are primarily processed into fertilizers and animal feed due to their high protein and mineral content. Despite the considerable volume of slaughter by-products generated, their application in cell culture systems and other high-value industries remains underdeveloped. Promoting the industrial utilization of these materials could not only improve resource efficiency and reduce environmental impact but also contribute to the sustainability and economic value of the livestock sector.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12995063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wei Ren, Lingyun Sun, Xinyi Du, Lu Meng, Muhammad Zahoor Khan, Changfa Wang, Mengmeng Li
{"title":"Impact of dietary soybean and linseed oils on the lipid and volatile compound profiles of donkey milk.","authors":"Wei Ren, Lingyun Sun, Xinyi Du, Lu Meng, Muhammad Zahoor Khan, Changfa Wang, Mengmeng Li","doi":"10.1007/s44463-025-00009-w","DOIUrl":"10.1007/s44463-025-00009-w","url":null,"abstract":"<p><p>Dietary oils significantly influence the modification of milk lipid profiles and volatile compounds (VOCs). In this study, donkeys were fed varying ratios of soybean oil to linseed oil, and the milk were analyzed for lipids and VOCs. A total of 1,167 lipid species were detected in donkey milk, belongs to 20 sub-classes. Sixty-four different lipids were screened and were most relevant to glycerophospholipid metabolism and linoleic acid metabolism. The level of C18:3 increased with higher linseed oil supplementation. In addition, 406 VOCs were identified in donkey milk, categorized into 15 sub-classes, mainly 80 hydrocarbons and 56 heterocyclic compounds. Eighty-nine differential VOCs were identified, mainly heterocyclic compounds, hydrocarbons, and alcohols. These VOCs were characterized by fruity, oily, grassy, and sweet aromas. A 2:3 dietary ratio of soybean oil to linseed oil significantly enhances the lipid quality and modulates the profile of VOCs in donkey milk. This study provides comprehensive insights on the impact of soybean and linseed oils blends on the lipid and VOC profiles, improving donkey milk's nutritional quality.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12995024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oxidative stability and growth-promoting effect for Lactobacillus acidophilus ATCC 43121 of whey protein isolate/inulin Maillard conjugates-based nanoemulsion delivery system containing fish oil.","authors":"Yeon-Woo Kim, Se-Hee Lee, Won-Jae Lee","doi":"10.1007/s44463-025-00032-x","DOIUrl":"10.1007/s44463-025-00032-x","url":null,"abstract":"<p><p>This study investigated the oxidative stability and growth-promoting effect for Lactobacillus acidophilus ATCC 43121 of a nanoemulsion delivery system (NDS) composed of whey protein isolate (WPI)/inulin Maillard conjugates. WPI/inulin Maillard conjugates prepared at pH 8.4 and a temperature of 85 °C had enhanced ABTS radical scavenging activity and were used as coating materials for the NDS. The WPI/inulin Maillard conjugates-based NDS exhibited a smaller droplet size than the WPI-based NDS. A droplet size of approximately 180 nm was observed in the NDS containing the WPI/inulin Maillard conjugates. The WPI/inulin Maillard conjugates-based NDS maintained a consistent droplet size and polydispersity index throughout 16 d of storage, indicating excellent physical stability. Compared to both fish oil encapsulated in NDS and WPI-based NDS, fish oil in WPI/inulin Maillard conjugates-based NDS demonstrated lower PV and p-anisidine value and reduced production of potential markers of oxidation during a 16 d storage period. This indicated that the WPI/inulin Maillard conjugates-based NDS had enhanced oxidative stability. The viability of L. acidophilus ATCC 43121 increased in minimum media supplemented with WPI/inulin Maillard conjugates-based NDS compared to WPI- or inulin-based NDS, suggesting that WPI/inulin Maillard conjugates-based NDS had a growth-promoting effect for L. acidophilus ATCC 43121. In conclusion, the use of WPI/inulin Maillard conjugates-based NDS is an effective way to enhance the oxidative stability of fish oil and its growth-promoting effect for L. acidophilus ATCC 43121.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12995098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Bong Lee, Ji Hyun Lim, Ji Heon Park, Hyeon Jeong Moon, Gun Lee, Chung-Do Lee, Jonghyun Kim, Ji-Young Park, Kwang Jun Lee, Soo-Jin Yang
{"title":"Serotype distribution, genotype, and antimicrobial resistance profiles of Salmonella enterica isolated from retail chicken meat in South Korea.","authors":"Jun Bong Lee, Ji Hyun Lim, Ji Heon Park, Hyeon Jeong Moon, Gun Lee, Chung-Do Lee, Jonghyun Kim, Ji-Young Park, Kwang Jun Lee, Soo-Jin Yang","doi":"10.1007/s44463-025-00042-9","DOIUrl":"10.1007/s44463-025-00042-9","url":null,"abstract":"<p><p>Salmonella is a leading cause of foodborne illnesses worldwide, with poultry serving as the primary reservoir of this pathogen. Contaminated poultry meat products and eggs are major sources of human salmonellosis. In this study, 85 Salmonella isolates were collected from 120 retail chicken meat products in Korea and their serotype distributions, genetic characteristics, and antimicrobial resistance (AMR) profiles were analyzed. We identified 59 isolates (69.4%) as (i) Salmonella Infantis, (ii) belonging to sequence type (ST) 32 or its single-locus variant, and (iii) possessing the plasmid of the emerging S. Infantis (pESI)-like megaplasmid carrying the bla<sub>CTX-M-65</sub> gene. Among the 26 non-Infantis Salmonella isolates, only eight were serotyped as S. Enteritidis, S. Montevideo, and S. Typhimurium, whereas 18 untypeable isolates were clustered into six STs. Notably, high levels of resistance to three or more antibiotic classes, including β-lactams, aminoglycosides, phenicol, sulfonamide, tetracycline, and quinolone, were observed among the S. Infantis isolates. Additionally, mutations in the quinolone resistance-determining region (QRDR) of gyrA were identified in fluoroquinolone-resistant Salmonella isolates. Collectively, these findings suggest that multidrug-resistant ST32 S. Infantis is widely disseminated among retail chickens in Korea. The emergence of pESI-like megaplasmid and the increased levels of multidrug resistance in S. Infantis poses a significant threat to public health. Consequently, continuous surveillance of AMR profiles and the underlying mechanisms in Salmonella is crucial.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"46 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12994942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}